{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:50:44Z","timestamp":1791226244433,"version":"4.1.0"},"reference-count":98,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003990","name":"Conseil R\u00e9gional, \u00cele-de-France","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003990","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FRM, Fondation pour la recherche m\u00e9dicale","id":[{"id":"https:\/\/ror.org\/04w6kn183","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-10-LABX-62-IBEID studentship"],"award-info":[{"award-number":["ANR-10-LABX-62-IBEID studentship"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-13-ISV3-0009"],"award-info":[{"award-number":["ANR-13-ISV3-0009"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    <jats:italic>Leishmania<\/jats:italic>\n                    CK1.2 (L-CK1.2) is a serine\/threonine protein kinase essential for the survival of the protozoan parasite\n                    <jats:italic>Leishmania<\/jats:italic>\n                    , the causative agent of leishmaniasis. This study investigates the dynamic localization pattern of L-CK1.2 and the broad spectrum of its interacting partners within the parasite. Using proteomic analysis and confocal microscopy, we identified 230 L-CK1.2-associated proteins across the major parasite life stages. This analysis revealed the ubiquitous presence of L-CK1.2 in various cellular structures, including the cytoskeleton, basal body, and flagellum. Using an\n                    <jats:italic>in vitro<\/jats:italic>\n                    system, sixty-four host proteins, involved in critical host biological processes such as immune response, apoptosis, and purine biosynthesis, were identified as potential interacting partners of L-CK1.2. These processes are known to be regulated by\n                    <jats:italic>Leishmania<\/jats:italic>\n                    during infection. The study highlights the potential dual function of L-CK1.2, in the parasite (\n                    <jats:italic>cis<\/jats:italic>\n                    ) and within the host cell (\n                    <jats:italic>trans<\/jats:italic>\n                    ), positioning this kinase as a key player in host-pathogen interactions. This work provides a comprehensive map of L-CK1.2 interactions and suggests its potential importance in regulating intracellular\n                    <jats:italic>Leishmania<\/jats:italic>\n                    survival, providing potential targets for leishmaniasis therapy. Furthermore, given the evolutionary conservation of CK1.2 across other parasitic organisms, our findings may have broader implications for understanding and managing parasitic infections.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0356818","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:52:51Z","timestamp":1791222771000},"page":"e0356818","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the dual functions of Leishmania CK1.2 in both the parasite and the macrophage"],"prefix":"10.1371","volume":"21","author":[{"given":"Daniel","family":"Martel","sequence":"first","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9 and INSERM U1347, Groupe de signalisation et interactions h\u00f4te-parasite","place":["Paris, France"]},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9 and INSERM U1347","place":["Paris, France"],"department":["Unit\u00e9 de Parasitologie mol\u00e9culaire et Signalisation"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Leclercq","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9 and INSERM U1347, Groupe de signalisation et interactions h\u00f4te-parasite","place":["Paris, France"]},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9 and INSERM U1347","place":["Paris, France"],"department":["Unit\u00e9 de Parasitologie mol\u00e9culaire et Signalisation"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7715-2446","authenticated-orcid":true,"given":"Florent","family":"Dingli","sequence":"additional","affiliation":[{"name":"Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique (LSMP), Centre de Recherche, Institut Curie, PSL Research University","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5739-5742","authenticated-orcid":true,"given":"Victor","family":"Laigle","sequence":"additional","affiliation":[{"name":"Laboratoire de Spectrom\u00e9trie de Masse Prot\u00e9omique (LSMP), Centre de Recherche, Institut Curie, PSL Research University","place":["Paris, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0385-543X","authenticated-orcid":true,"given":"Nawal","family":"Hajj Sleiman","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9 and INSERM U1347, Groupe de signalisation et interactions h\u00f4te-parasite","place":["Paris, France"]},{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9 and INSERM U1347","place":["Paris, France"],"department":["Unit\u00e9 de Parasitologie mol\u00e9culaire et 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