{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:09:40Z","timestamp":1789150180693,"version":"build-2803163510"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"International Science and Technology Cooperation Platform of BAAFS","award":["Grant No. GHPT2026-13"],"award-info":[{"award-number":["Grant No. GHPT2026-13"]}]},{"name":"National Key Research and Development Program of China","award":["Grant No. 2025YFE0104800"],"award-info":[{"award-number":["Grant No. 2025YFE0104800"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Wheat (\n                    <jats:italic>Triticum aestivum L<\/jats:italic>\n                    .) yield in Pakistan has remained stagnant over the past two decades mainly due to the narrow genetic diversity of cultivated varieties. To overcome this limitation and explore new genetic resources for improved productivity, twelve Chinese wheat hybrid lines were evaluated in comparison with two local cultivars at the Agronomy Research Farm, the University of Agriculture Peshawar, Pakistan over two seasons (2021\u20132022, 2022\u20132023). Results showed that early emergence occurred in the hybrid lines 20 BH 37 and 20 BH 53 (12.5 days). Wheat lines 20 BH 53 and 20 BH 56 produced higher number of tillers m\n                    <jats:sup>-2<\/jats:sup>\n                    (441 and 412), spike density (396 and 391 spikes m\n                    <jats:sup>-2<\/jats:sup>\n                    ), thousand grain weight (41.9 g and 43.5 g), grains spike\n                    <jats:sup>-1<\/jats:sup>\n                    (54 and 56), biological yield (9561 and 9306 kg ha\n                    <jats:sup>-1<\/jats:sup>\n                    ), and grain yield (4253 and 4225 kg ha\n                    <jats:sup>-1<\/jats:sup>\n                    ), respectively. Hybrid lines 20 BH 53 and 20 BH 56 also exhibited a 17\u201318% increase in grain yield over Wadan-17 and a 16\u201317% increase over Akbar-19, highlighting their potential as superior genetic resources for wheat productivity enhancement. Positive correlation was observed for grain yield and yield components. Principal component analysis revealed considerable genetic diversity among the tested hybrid lines, supporting their inclusion in future breeding programs. It is concluded that wheat hybrid lines 20 BH 53 and 20 BH 56 outperformed in yield and yield traits over local cultivars under semiarid conditions, indicating their suitability for agro-ecological conditions of northern parts of Pakistan and for future breeding programs.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0356875","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:35:17Z","timestamp":1789148117000},"page":"e0356875","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unlocking wheat yield potential through evaluation of high-performing hybrid lines in semiarid conditions of northern Pakistan"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6247-1320","authenticated-orcid":true,"given":"Hamid Ali","family":"Khan","sequence":"first","affiliation":[{"name":"The University of Agriculture","place":["Peshawar, Pakistan"],"department":["Department of Agronomy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Arif","sequence":"additional","affiliation":[{"name":"The University of Agriculture","place":["Peshawar, Pakistan"],"department":["Department of Agronomy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5029-6415","authenticated-orcid":true,"given":"Shengquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Science","place":["Beijing, China"],"department":["Institute of Hybrid Wheat"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fazal","family":"Munsif","sequence":"additional","affiliation":[{"name":"The University of Agriculture","place":["Peshawar, Pakistan"],"department":["Department of Agronomy, Amir Muhammad Khan Campus Mardan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyao","family":"Lou","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Science","place":["Beijing, China"],"department":["Institute of Hybrid Wheat"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutian","family":"Gao","sequence":"additional","affiliation":[{"name":"Beijing Academy of Agriculture and Forestry Science","place":["Beijing, China"],"department":["Institute of Hybrid Wheat"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4999-053X","authenticated-orcid":true,"given":"Basit","family":"Ullah","sequence":"additional","affiliation":[{"name":"The University of Agriculture","place":["Peshawar, Pakistan"],"department":["Department of Agronomy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Mehran","family":"Anjum","sequence":"additional","affiliation":[{"name":"The University of Agriculture","place":["Peshawar, Pakistan"],"department":["Department of Agronomy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maaz","family":"Khan","sequence":"additional","affiliation":[{"name":"The University of Agriculture","place":["Peshawar, Pakistan"],"department":["Department of Agronomy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beena","family":"Saeed","sequence":"additional","affiliation":[{"name":"University of Swabi","place":["Pakhtunkhwa, Pakistan"],"department":["Department of Agriculture"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"issue":"1","key":"pone.0356875.ref001","doi-asserted-by":"crossref","first-page":"50","DOI":"10.54910\/sabrao2023.55.1.5","article-title":"Stability analysis of wheat through genotype by environment interaction in three regions of Khyber Pakhtunkhwa, Pakistan","volume":"55","author":"I 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of Soil Science","author":"HD Foth","year":"1982","edition":"6"},{"key":"pone.0356875.ref022","author":"ML Jackson","year":"1969","journal-title":"Soil chemical analysis-advanced course"},{"key":"pone.0356875.ref023","volume-title":"Estimation of available phosphorus in soils by extraction with sodium bicarbonate","author":"SR Olsen","year":"1954"},{"issue":"3","key":"pone.0356875.ref024","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/00103627709366714","article-title":"A new soil test for simultaneous extraction of macro\u2010 and micro\u2010nutrients in alkaline soils","volume":"8","author":"PN Soltanpour","year":"1977","journal-title":"Communications in Soil Science and Plant Analysis"},{"issue":"1","key":"pone.0356875.ref025","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1097\/00010694-193401000-00003","article-title":"An examination of the degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method","volume":"37","author":"A Walkley","year":"1934","journal-title":"Soil Science"},{"key":"pone.0356875.ref026","author":"RGD Steel","year":"1960","journal-title":"Principles and procedures of statistics"},{"issue":"1","key":"pone.0356875.ref027","doi-asserted-by":"crossref","first-page":"114","DOI":"10.62997\/rl.2025.41046","article-title":"An Integrated Assessment of Climatic and Non-Climatic Determinants of Wheat Production and Food Security in Pakistan","volume":"4","author":"Dr Pervaiz B","year":"2025","journal-title":"RL"},{"issue":"4","key":"pone.0356875.ref028","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1007\/s42106-024-00306-2","article-title":"Hybrid Winter Wheat Performance: A Study on Planting Density, Nitrogen Use Efficiency, and Yield Optimization in Eastern China","volume":"18","author":"Y Zhang","year":"2024","journal-title":"Int J Plant 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Singh","year":"2024","journal-title":"Plants (Basel)"},{"issue":"1","key":"pone.0356875.ref036","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1590\/S0103-90162009000100004","article-title":"Seeding density in wheat genotypes as a function of tillering potential","volume":"66","author":"IP Val\u00e9rio","year":"2009","journal-title":"Sci agric (Piracicaba, Braz)"},{"key":"pone.0356875.ref037","doi-asserted-by":"crossref","first-page":"961","DOI":"10.2135\/cropsci2008.05.0285","article-title":"Relationships between large\u2010spike phenotype, grain number, and yield potential in spring wheat","volume":"49","author":"O Gaju","year":"2009","journal-title":"Crop Science"},{"issue":"10","key":"pone.0356875.ref038","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0205452","article-title":"Grain number and grain yield distribution along the spike remain stable despite breeding for high yield in winter wheat","volume":"13","author":"N Philipp","year":"2018","journal-title":"PLoS One"},{"key":"pone.0356875.ref039","article-title":"Improving grain yield in wheat (Triticum aestivum L.) by stem reserve remobilization under heat stress","volume":"127","author":"MA Ahmad","year":"2016","journal-title":"Plant Stress Tolerance Physiological Mol Strategies"},{"key":"pone.0356875.ref040","first-page":"334","article-title":"Effect of temperature on stem reserve mobilization for grain development in wheat","volume":"43","author":"S Gare","year":"2018","journal-title":"Journal of Agricultural Research and Technology"},{"issue":"4","key":"pone.0356875.ref041","doi-asserted-by":"crossref","first-page":"300","DOI":"10.15547\/ast.2019.04.050","article-title":"Heterosis manifestations for spike productivity traits in durum wheat","volume":"11","author":"R 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