{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:16:57Z","timestamp":1787941017366,"version":"build-2784847793"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T00:00:00Z","timestamp":1787875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Phycocyanobilin, a bioactive compound derived from\n                    <jats:italic>Arthrospira platensis<\/jats:italic>\n                    C1, was investigated for its potential role in systemic lupus erythematosus (SLE) based on its structural similarity to bilirubin, with a Tanimoto score of 93%. Molecular docking revealed favorable binding affinities between phycocyanobilin and several protein targets, including EGFR, FYN, HLA-B, LCK, LYN, and TP53. Target prediction further identified LYN kinase as a key candidate. Molecular dynamics simulations demonstrated stable binding of the phycocyanobilin\u2013LYN complex, with interaction profiles comparable to those of the native ligand, staurosporine. Binding free energy and residue-level analyses supported strong and stable interactions, highlighting key contributions to complex stability. Overall, these findings provide mechanistic insight into the interaction between phycocyanobilin and LYN, suggesting that this compound may modulate LYN-associated signaling pathways and warrants further investigation in the context of SLE.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0357093","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T17:23:59Z","timestamp":1787937839000},"page":"e0357093","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phycocyanobilin: A potential bioactive compound from Arthrospira platensis targeting LYN protein associated with systemic lupus erythematosus"],"prefix":"10.1371","volume":"21","author":[{"given":"Amnart","family":"Chaiprasert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3194-1391","authenticated-orcid":true,"given":"Teeraphan","family":"Laomettachit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2872-7757","authenticated-orcid":true,"given":"Marasri","family":"Ruengjitchatchawalya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,8,28]]},"reference":[{"issue":"22","key":"pone.0357093.ref001","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1056\/NEJMra1100359","article-title":"Systemic lupus erythematosus","volume":"365","author":"GC Tsokos","year":"2011","journal-title":"N Engl J Med"},{"issue":"12","key":"pone.0357093.ref002","doi-asserted-by":"crossref","first-page":"2009","DOI":"10.1136\/annrheumdis-2017-211663","article-title":"Survival in adults and children with systemic lupus erythematosus: a systematic review and Bayesian meta-analysis of studies from 1950 to 2016","volume":"76","author":"MG Tektonidou","year":"2017","journal-title":"Ann Rheum Dis"},{"issue":"6","key":"pone.0357093.ref003","doi-asserted-by":"crossref","first-page":"480","DOI":"10.3109\/03009741003742748","article-title":"Association of systemic lupus erythematosus with low serum bilirubin levels","volume":"39","author":"L V\u00edtek","year":"2010","journal-title":"Scand J Rheumatol"},{"issue":"8","key":"pone.0357093.ref004","doi-asserted-by":"crossref","first-page":"2423","DOI":"10.1007\/s00296-011-1977-9","article-title":"Bilirubin levels in patients with systemic lupus erythematosus: increased or decreased?","volume":"32","author":"Z Yang","year":"2012","journal-title":"Rheumatol Int"},{"issue":"4","key":"pone.0357093.ref005","article-title":"The relationship between bilirubin levels and patients with lupus nephritis","volume":"65","author":"Q He","year":"2019","journal-title":"Clin Lab"},{"issue":"12","key":"pone.0357093.ref006","article-title":"Relationship between bilirubin and systemic lupus erythematosus: a systematic review and meta-analysis","volume":"11","author":"Y Yu","year":"2023","journal-title":"Immun Inflamm Dis"},{"issue":"4","key":"pone.0357093.ref007","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1002\/med.21660","article-title":"Bilirubin as a signaling molecule","volume":"40","author":"L V\u00edtek","year":"2020","journal-title":"Med Res Rev"},{"key":"pone.0357093.ref008","unstructured":"Spirulina phycobiliproteins as food components and complements. IntechOpen. https:\/\/www.intechopen.com\/chapters\/59210"},{"key":"pone.0357093.ref009","doi-asserted-by":"crossref","first-page":"110314","DOI":"10.1016\/j.foodres.2021.110314","article-title":"Phycocyanin from Spirulina: A review of extraction methods and stability","volume":"143","author":"D Pez Jaeschke","year":"2021","journal-title":"Food Res Int"},{"issue":"11","key":"pone.0357093.ref010","doi-asserted-by":"crossref","first-page":"1752","DOI":"10.3390\/nu16111752","article-title":"Nutraceutical features of the phycobiliprotein c-phycocyanin: evidence from arthrospira platensis (spirulina)","volume":"16","author":"V Citi","year":"2024","journal-title":"Nutrients"},{"issue":"2","key":"pone.0357093.ref011","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/s11418-008-0308-2","article-title":"Appraisal of immunomodulatory potential of Spirulina fusiformis: an in vivo and in vitro study","volume":"63","author":"M Rasool","year":"2009","journal-title":"J Nat Med"},{"issue":"3","key":"pone.0357093.ref012","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1038\/cmi.2010.76","article-title":"The effects of Spirulina on anemia and immune function in senior citizens","volume":"8","author":"C Selmi","year":"2011","journal-title":"Cell Mol Immunol"},{"issue":"3","key":"pone.0357093.ref013","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s10811-011-9766-0","article-title":"Dietary algae and HIV\/AIDS: proof of concept clinical data","volume":"24","author":"J Teas","year":"2012","journal-title":"J Appl Phycol"},{"key":"pone.0357093.ref014","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/1471-230X-12-32","article-title":"Spirulina platensis versus silymarin in the treatment of chronic hepatitis C virus infection. A pilot randomized, comparative clinical trial","volume":"12","author":"M Yakoot","year":"2012","journal-title":"BMC Gastroenterol"},{"issue":"4","key":"pone.0357093.ref015","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1089\/10966200152744463","article-title":"Role of spirulina in the control of glycemia and lipidemia in type 2 diabetes mellitus","volume":"4","author":"P Parikh","year":"2001","journal-title":"J Med Food"},{"issue":"10","key":"pone.0357093.ref016","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1007\/s00405-008-0642-8","article-title":"The effects of spirulina on allergic rhinitis","volume":"265","author":"C Cingi","year":"2008","journal-title":"Eur Arch Otorhinolaryngol"},{"key":"pone.0357093.ref017","doi-asserted-by":"crossref","first-page":"531053","DOI":"10.1093\/ecam\/nen058","article-title":"Spirulina in clinical practice: evidence-based human applications","volume":"2011","author":"PD Karkos","year":"2011","journal-title":"Evid Based Complement Alternat Med"},{"issue":"2","key":"pone.0357093.ref018","first-page":"91","article-title":"Phycocyanin from arthrospira platensis as potential anti-cancer drug: review of in vitro and in vivo studies","volume":"11","author":"S Braune","year":"2021","journal-title":"Life (Basel)"},{"key":"pone.0357093.ref019","doi-asserted-by":"crossref","first-page":"111089","DOI":"10.1016\/j.nut.2020.111089","article-title":"Prospective options of algae-derived nutraceuticals as supplements to combat COVID-19 and human coronavirus diseases","volume":"83","author":"SK Ratha","year":"2021","journal-title":"Nutrition"},{"issue":"8","key":"pone.0357093.ref020","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0309303","article-title":"Network analysis retrieving bioactive compounds from Spirulina (Arthrospira platensis) and their targets related to systemic lupus erythematosus","volume":"19","author":"A Chaiprasert","year":"2024","journal-title":"PLoS One"},{"key":"pone.0357093.ref021","unstructured":"PubChem. https:\/\/pubchem.ncbi.nlm.nih.gov\/. Accessed 2024 September 27."},{"key":"pone.0357093.ref022","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/1752-0509-6-71","article-title":"iAK692: a genome-scale metabolic model of Spirulina platensis C1","volume":"6","author":"A Klanchui","year":"2012","journal-title":"BMC Syst Biol"},{"issue":"1","key":"pone.0357093.ref023","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1186\/s12859-015-0676-z","article-title":"SpirPro: a Spirulina proteome database and web-based tools for the analysis of protein-protein interactions at the metabolic level in Spirulina (Arthrospira) platensis C1","volume":"16","author":"J Senachak","year":"2015","journal-title":"BMC Bioinformatics"},{"issue":"13","key":"pone.0357093.ref024","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1002\/jcc.20084","article-title":"UCSF Chimera--a visualization system for exploratory research and analysis","volume":"25","author":"EF Pettersen","year":"2004","journal-title":"J Comput Chem"},{"issue":"2","key":"pone.0357093.ref025","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3177\/jnsv.50.129","article-title":"Phycocyanin enhances secretary IgA antibody response and suppresses allergic IgE antibody response in mice immunized with antigen-entrapped biodegradable microparticles","volume":"50","author":"C Nemoto-Kawamura","year":"2004","journal-title":"J Nutr Sci Vitaminol (Tokyo)"},{"key":"pone.0357093.ref026","doi-asserted-by":"crossref","first-page":"401","DOI":"10.3389\/fimmu.2018.00401","article-title":"Lyn, Lupus, and (B) Lymphocytes, a Lesson on the Critical Balance of Kinase Signaling in Immunity","volume":"9","author":"EJ Brodie","year":"2018","journal-title":"Front Immunol"},{"issue":"3","key":"pone.0357093.ref027","doi-asserted-by":"crossref","first-page":"919","DOI":"10.4049\/jimmunol.1301979","article-title":"B cell-specific loss of Lyn kinase leads to autoimmunity","volume":"192","author":"C Lamagna","year":"2014","journal-title":"J Immunol"},{"issue":"1","key":"pone.0357093.ref028","doi-asserted-by":"crossref","first-page":"2515532","DOI":"10.1080\/0886022X.2025.2515532","article-title":"Association between Lyn tyrosine kinase and renal injury in patients with systemic lupus erythematosus","volume":"47","author":"J Sun","year":"2025","journal-title":"Ren Fail"},{"key":"pone.0357093.ref029","unstructured":"SwissADME. https:\/\/www.swissadme.ch\/. Accessed 2026 April 25."},{"key":"pone.0357093.ref030","unstructured":"ADMETlab 3.0. https:\/\/admetlab3.scbdd.com\/. Accessed 2026 April 25."},{"key":"pone.0357093.ref031","unstructured":"ProTox-3.0 - Prediction of TOXicity of chemicals. https:\/\/tox.charite.de\/protox3\/index.php?site=compound_search_similarity. 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