{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:11:09Z","timestamp":1789755069652,"version":"4.0.1"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/18886-1"],"award-info":[{"award-number":["2019\/18886-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.676144\/2022-00"],"award-info":[{"award-number":["88887.676144\/2022-00"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Chronic hemolytic anemia found in patients with sickle cell disease promotes an excess of free extracellular and intravascular hemoglobin, triggering a series of deleterious effects such as endothelial dysfunction, oxidative stress, and alterations in vascular tone, which lead to clinical complications. In this work, we evaluated the induced expression of haptoglobin in hepatocytes in vivo, the organism\u2019s first line of defense to control the presence of free Hb in circulation, which becomes saturated in conditions of chronic hemolysis, such as sickle cell disease. Through gene therapy applied in a murine model of sickle cell anemia, we aimed to investigate whether this approach could interfere with disease progression and promote an improvement in the animals\u2019 pathophysiological condition. The application of this treatment in the sickle cell anemia animal model resulted in significant therapeutic effects, including improvement of hematological parameters, reduction of the compensatory erythropoietic response, and attenuation of clinical signs such as splenomegaly. These results indicate that haptoglobin may act indirectly in the prevention of hemolysis, suggesting a new therapeutic approach.<\/jats:p>","DOI":"10.1371\/journal.pone.0357180","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:50:03Z","timestamp":1789753803000},"page":"e0357180","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic potential of haptoglobin in a murine model of sickle cell anemia"],"prefix":"10.1371","volume":"21","author":[{"given":"Bianca Cristina","family":"dos Santos","sequence":"first","affiliation":[{"name":"Laboratory for the Development of Biological Models, Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP)","place":["Campinas, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnun Nueldo Nunes","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Hemoglobinopathies Laboratory, School of Medical Sciences, University of Campinas (UNICAMP)","place":["Campinas, Brazil"],"department":["Department of Pathology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Audrei","family":"Pedroso","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, Clinical Pathology Laboratory (LPC), University Hospital, School of Medical Sciences, University of Campinas (UNICAMP)","place":["Campinas, Brazil"],"department":["Department of Pathology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Benedetti","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Hematology Laboratory, Clinical Pathology Laboratory (LPC), University Hospital, School of Medical Sciences, University of Campinas (UNICAMP)","place":["Campinas, Brazil"],"department":["Department of Pathology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulcin\u00e9ia Martins","family":"de Albuquerque","sequence":"additional","affiliation":[{"name":"Center for Hematology and Hemotherapy, National Institute of Science and Technology in Blood (INCTS), University of Campinas (UNICAMP)","place":["Campinas, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalia Rocha","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory for the Development of Biological Models, Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP)","place":["Campinas, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Cagnoto","family":"Noronha","sequence":"additional","affiliation":[{"name":"Laboratory for the Development of Biological Models, Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP)","place":["Campinas, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Ferreira","family":"Costa","sequence":"additional","affiliation":[{"name":"Center for Hematology and Hemotherapy, National Institute of Science and Technology in Blood (INCTS), University of Campinas (UNICAMP)","place":["Campinas, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8801-2318","authenticated-orcid":true,"given":"Marcus Alexandre Finzi","family":"Corat","sequence":"additional","affiliation":[{"name":"Laboratory for the Development of Biological Models, Multidisciplinary Center for Biological Research, University of Campinas (UNICAMP)","place":["Campinas, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"pone.0357180.ref001","doi-asserted-by":"crossref","first-page":"624477","DOI":"10.3389\/fcell.2020.624477","article-title":"In utero therapy 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