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China","award":["(2023)94"],"award-info":[{"award-number":["(2023)94"]}]},{"name":"Key Discipline Project at the School of Public Health, Chengdu Medical College","award":["21"],"award-info":[{"award-number":["21"]}],"id":[{"id":"https:\/\/ror.org\/01c4jmp52","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The giant panda (\n                    <jats:italic>Ailuropoda melanoleuca<\/jats:italic>\n                    ) is a relict species endemic to China. Captive breeding has boosted its population. However, some captive individuals exhibit stereotypic behavior. Though extensive research showing that host behavior can be influenced by gut microbiota via the gut-brain axis, the gut microbiota related to stereotypic behavior of giant pandas has not been reported. To explore gut microbial composition and functional characteristics of stereotypically behaving (SB) giant pandas, the behavior of 15 captive giant pandas was observed, and their feces were analyzed using 16S ribosomal RNA (rRNA) sequencing (n\u2009=\u2009145) and metagenomic sequencing (n\u2009=\u200945). Our study revealed distinct gut microbial diversity, composition, and functions between SB giant pandas and normal controls, indicating gut microbial dysbiosis in SB giant pandas.\n                    <jats:italic>Serratia<\/jats:italic>\n                    sp.\n                    <jats:italic>Se-RSBMAAmG<\/jats:italic>\n                    was significantly enriched in SB giant pandas, five\n                    <jats:italic>Clostridium<\/jats:italic>\n                    species and\n                    <jats:italic>Lactococcus garvieae<\/jats:italic>\n                    exhibited upward trends in SB individuals, whereas the probiotic\n                    <jats:italic>Enterococcus hirae<\/jats:italic>\n                    showed the opposite trend compared with those in normal controls. The\n                    <jats:italic>Clostridium<\/jats:italic>\n                    bacteria may affect propionate metabolism and the gamma-aminobutyric acidergic (GABAergic) synapse pathway, potentially affecting the emergence of stereotypic behavior. These findings suggest a link between gut microbiota and stereotypic behavior of giant pandas, providing novel insights for their conservation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0357625","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:23:31Z","timestamp":1790184211000},"page":"e0357625","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut microbiome is associated with stereotypic behavior of the giant panda (Ailuropoda melanoleuca)"],"prefix":"10.1371","volume":"21","author":[{"given":"Anqi","family":"Hu","sequence":"first","affiliation":[{"name":"The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding","place":["Chengdu, Sichuan, China"]},{"name":"College of Veterinary Medicine, Jilin Agricultural University","place":["Changchun, Jilin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Chengdu University","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuixing","family":"Yang","sequence":"additional","affiliation":[{"name":"The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Public Health, Chengdu Medical College","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gai","family":"Luo","sequence":"additional","affiliation":[{"name":"The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin Agricultural University","place":["Changchun, Jilin, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1230-5405","authenticated-orcid":true,"given":"Mengnan","family":"He","sequence":"additional","affiliation":[{"name":"The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding","place":["Chengdu, Sichuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"issue":"38","key":"pone.0357625.ref001","article-title":"Generation and characterization of giant panda induced pluripotent stem cells","volume":"10","author":"Y Liu","year":"2024","journal-title":"Sci Adv"},{"key":"pone.0357625.ref002","doi-asserted-by":"crossref","first-page":"142975","DOI":"10.1016\/j.scitotenv.2020.142975","article-title":"A review of the impacts of four identified major human disturbances on the habitat and habitat use of wild giant pandas from 2015 to 2020","volume":"763","author":"D Kang","year":"2021","journal-title":"Sci Total Environ"},{"key":"pone.0357625.ref003","article-title":"Panda downlisted but not out of the woods","volume":"11","author":"RR Swaisgood","year":"2017","journal-title":"Conserv Lett"},{"issue":"1","key":"pone.0357625.ref004","article-title":"Effects of Ambient Environmental Factors on the Stereotypic Behaviors of Giant Pandas (Ailuropoda melanoleuca)","volume":"12","author":"H Liu","year":"2017","journal-title":"PLoS One"},{"key":"pone.0357625.ref005","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0376-6357(91)90013-P","article-title":"Stereotypies and suffering","volume":"25","author":"GJ Mason","year":"1991","journal-title":"Behav Processes"},{"issue":"1","key":"pone.0357625.ref006","doi-asserted-by":"crossref","first-page":"7263","DOI":"10.1038\/s41598-020-63763-5","article-title":"Stereotypic behaviour predicts reproductive performance and litter sex ratio in giant pandas","volume":"10","author":"MS Martin","year":"2020","journal-title":"Sci Rep"},{"issue":"6","key":"pone.0357625.ref007","first-page":"1776","article-title":"Behavioral, physiological and functional aspects of stereotyped behavior: a review and a re-interpretation","volume":"62","author":"R Dantzer","year":"1986","journal-title":"J Anim 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Collins","year":"2009","journal-title":"Gastroenterology"},{"issue":"1","key":"pone.0357625.ref011","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.bbi.2009.05.058","article-title":"Mood and gut feelings","volume":"24","author":"P Forsythe","year":"2010","journal-title":"Brain Behav Immun"},{"issue":"7","key":"pone.0357625.ref012","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1073\/pnas.1010529108","article-title":"Normal gut microbiota modulates brain development and behavior","volume":"108","author":"R Diaz Heijtz","year":"2011","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"pone.0357625.ref013","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1139\/gen-2020-0136","article-title":"Gut microbiome-mediated epigenetic regulation of brain disorder and application of machine learning for multi-omics data analysis","volume":"64","author":"H Kaur","year":"2021","journal-title":"Genome"},{"key":"pone.0357625.ref014","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1007\/978-1-4939-0897-4_17","article-title":"The impact of microbiota on brain and behavior: mechanisms & therapeutic potential","volume":"817","author":"YE Borre","year":"2014","journal-title":"Adv Exp Med Biol"},{"issue":"6","key":"pone.0357625.ref015","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2019.05.004","article-title":"Human Gut Microbiota from Autism Spectrum Disorder Promote Behavioral Symptoms in Mice","volume":"177","author":"G Sharon","year":"2019","journal-title":"Cell"},{"key":"pone.0357625.ref016","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.jocn.2022.03.003","article-title":"An anxious relationship between Autism Spectrum Disorder and Gut Microbiota: A tangled chemistry?","volume":"99","author":"B Vellingiri","year":"2022","journal-title":"J Clin 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Utilization and Gut Microbiota Composition in Giant Pandas of Different Age Groups","volume":"14","author":"C Wang","year":"2024","journal-title":"Animals (Basel)"},{"key":"pone.0357625.ref024","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1515\/biol-2019-0032","article-title":"Comparative Analysis of Gut Microbiota Among the Male, Female and Pregnant Giant Pandas (Ailuropoda Melanoleuca)","volume":"14","author":"S Zhao","year":"2019","journal-title":"Open Life Sci"},{"issue":"21","key":"pone.0357625.ref025","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"T Mago\u010d","year":"2011","journal-title":"Bioinformatics"},{"issue":"1","key":"pone.0357625.ref026","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/nmeth.2276","article-title":"Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing","volume":"10","author":"NA Bokulich","year":"2013","journal-title":"Nat Methods"},{"issue":"16","key":"pone.0357625.ref027","doi-asserted-by":"crossref","first-page":"2194","DOI":"10.1093\/bioinformatics\/btr381","article-title":"UCHIME improves sensitivity and speed of chimera detection","volume":"27","author":"RC Edgar","year":"2011","journal-title":"Bioinformatics"},{"key":"pone.0357625.ref028","doi-asserted-by":"crossref","first-page":"754788","DOI":"10.3389\/fonc.2021.754788","article-title":"The Intratumor Microbiota Signatures Associate With Subtype, Tumor Stage, and Survival Status of Esophageal Carcinoma","volume":"11","author":"Y Wang","year":"2021","journal-title":"Front Oncol"},{"issue":"6","key":"pone.0357625.ref029","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2011-12-6-r60","article-title":"Metagenomic biomarker discovery and explanation","volume":"12","author":"N Segata","year":"2011","journal-title":"Genome Biol"},{"key":"pone.0357625.ref030","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1038\/ncomms2266","article-title":"Symptomatic atherosclerosis is associated with an altered gut metagenome","volume":"3","author":"FH Karlsson","year":"2012","journal-title":"Nat Commun"},{"issue":"7452","key":"pone.0357625.ref031","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1038\/nature12198","article-title":"Gut metagenome in European women with normal, impaired and diabetic glucose control","volume":"498","author":"FH Karlsson","year":"2013","journal-title":"Nature"},{"key":"pone.0357625.ref032","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.01202","article-title":"Expansion of intestinal Prevotella copri correlates with enhanced susceptibility to arthritis","volume":"2","author":"JU Scher","year":"2013","journal-title":"Elife"},{"issue":"8","key":"pone.0357625.ref033","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/nbt.2939","article-title":"Identification and assembly of genomes and genetic elements in complex metagenomic samples without using reference genomes","volume":"32","author":"HB Nielsen","year":"2014","journal-title":"Nat Biotechnol"},{"issue":"7285","key":"pone.0357625.ref034","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature08821","article-title":"A human gut microbial gene catalogue established by metagenomic sequencing","volume":"464","author":"J Qin","year":"2010","journal-title":"Nature"},{"issue":"10","key":"pone.0357625.ref035","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph","volume":"31","author":"D Li","year":"2015","journal-title":"Bioinformatics"},{"issue":"2","key":"pone.0357625.ref036","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0031386","article-title":"Assessment of metagenomic assembly using simulated next generation sequencing data","volume":"7","author":"DR Mende","year":"2012","journal-title":"PLoS One"},{"issue":"8","key":"pone.0357625.ref037","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1038\/nbt.2942","article-title":"An integrated catalog of reference genes in the human gut microbiome","volume":"32","author":"J Li","year":"2014","journal-title":"Nat Biotechnol"},{"issue":"7520","key":"pone.0357625.ref038","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature13786","article-title":"Biogeography and individuality shape function in the human skin metagenome","volume":"514","author":"NISC Comparative Sequencing Program","year":"2014","journal-title":"Nature"},{"issue":"7516","key":"pone.0357625.ref039","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature13568","article-title":"Alterations of the human gut microbiome in liver cirrhosis","volume":"513","author":"N Qin","year":"2014","journal-title":"Nature"},{"issue":"11","key":"pone.0357625.ref040","doi-asserted-by":"crossref","first-page":"766","DOI":"10.15252\/msb.20145645","article-title":"Potential of fecal microbiota for early-stage detection of colorectal cancer","volume":"10","author":"G Zeller","year":"2014","journal-title":"Mol Syst Biol"},{"issue":"13","key":"pone.0357625.ref041","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"W Li","year":"2006","journal-title":"Bioinformatics"},{"issue":"23","key":"pone.0357625.ref042","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1093\/bioinformatics\/bts565","article-title":"CD-HIT: accelerated for clustering the next-generation sequencing data","volume":"28","author":"L Fu","year":"2012","journal-title":"Bioinformatics"},{"issue":"1","key":"pone.0357625.ref043","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nmeth.3176","article-title":"Fast and sensitive protein alignment using DIAMOND","volume":"12","author":"B Buchfink","year":"2015","journal-title":"Nat Methods"},{"issue":"9","key":"pone.0357625.ref044","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1101\/gr.120618.111","article-title":"Integrative analysis of environmental sequences using MEGAN4","volume":"21","author":"DH Huson","year":"2011","journal-title":"Genome Res"},{"key":"pone.0357625.ref045","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkj102","article-title":"From genomics to chemical genomics: new developments in KEGG","volume":"34","author":"M Kanehisa","year":"2006","journal-title":"Nucleic Acids Res"},{"key":"pone.0357625.ref046","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkw1092","article-title":"KEGG: new perspectives on genomes, pathways, diseases and drugs","volume":"45","author":"M Kanehisa","year":"2017","journal-title":"Nucleic Acids Res"},{"issue":"7418","key":"pone.0357625.ref047","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nature11450","article-title":"A metagenome-wide association study of gut microbiota in type 2 diabetes","volume":"490","author":"J Qin","year":"2012","journal-title":"Nature"},{"issue":"6","key":"pone.0357625.ref048","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1016\/j.chom.2015.05.012","article-title":"Dynamics and Stabilization of the Human Gut Microbiome during the First Year of Life","volume":"17","author":"F B\u00e4ckhed","year":"2015","journal-title":"Cell Host Microbe"},{"key":"pone.0357625.ref049","doi-asserted-by":"crossref","first-page":"115753","DOI":"10.1016\/j.biopha.2023.115753","article-title":"Insights into DNMT1 and programmed cell death in diseases","volume":"168","author":"L Yan","year":"2023","journal-title":"Biomed Pharmacother"},{"issue":"1","key":"pone.0357625.ref050","doi-asserted-by":"crossref","first-page":"4798","DOI":"10.1038\/s41467-021-25081-w","article-title":"Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health","volume":"12","author":"TPN Bui","year":"2021","journal-title":"Nat Commun"},{"issue":"10","key":"pone.0357625.ref051","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1016\/j.dld.2019.06.006","article-title":"Functional analysis of gut microbiota and immunoinflammation in children with autism spectrum disorders","volume":"51","author":"C Carissimi","year":"2019","journal-title":"Dig Liver Dis"},{"issue":"4","key":"pone.0357625.ref052","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1099\/jmm.0.001178","article-title":"The bacterial neurometabolic signature of the gut microbiota of young children with autism spectrum disorders","volume":"69","author":"OV Averina","year":"2020","journal-title":"J Med Microbiol"},{"issue":"3","key":"pone.0357625.ref053","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3390\/ani15030435","article-title":"Linking Gut Microbiota and Stereotypic Behavior of Endangered Species Under Ex Situ Conservation: First Evidence from Sun Bears","volume":"15","author":"X Chen","year":"2025","journal-title":"Animals (Basel)"},{"key":"pone.0357625.ref054","doi-asserted-by":"crossref","first-page":"644540","DOI":"10.3389\/fmicb.2021.644540","article-title":"Motor Stereotypic Behavior Was Associated With Immune Response in Macaques: Insight From Transcriptome and Gut Microbiota Analysis","volume":"12","author":"X Pan","year":"2021","journal-title":"Front Microbiol"},{"issue":"1","key":"pone.0357625.ref055","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.gpb.2019.01.002","article-title":"Correlation of Gut Microbiome Between ASD Children and Mothers and Potential Biomarkers for Risk Assessment","volume":"17","author":"N Li","year":"2019","journal-title":"Genomics Proteomics Bioinformatics"},{"issue":"13","key":"pone.0357625.ref056","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.3390\/ani15131954","article-title":"Impacts of Captive Domestication and Geographical Divergence on the Gut Microbiome of Endangered Forest Musk Deer","volume":"15","author":"H Liu","year":"2025","journal-title":"Animals (Basel)"},{"issue":"4","key":"pone.0357625.ref057","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1007\/s11274-024-03947-7","article-title":"Lactococcus garvieae exerts a critical role in inducing inflammation in dairy mastitis by triggering NLRP3 inflammasome-mediated pyroptosis in MAC-T cells","volume":"40","author":"F Wu","year":"2024","journal-title":"World J Microbiol Biotechnol"},{"issue":"3","key":"pone.0357625.ref058","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.3390\/ijms24031819","article-title":"Autism Spectrum Disorder: Neurodevelopmental Risk Factors, Biological Mechanism, and Precision Therapy","volume":"24","author":"L Wang","year":"2023","journal-title":"Int J Mol Sci"},{"issue":"7","key":"pone.0357625.ref059","doi-asserted-by":"crossref","first-page":"2111","DOI":"10.1007\/s11274-014-1625-0","article-title":"Antibacterial, anti-inflammatory and probiotic potential of Enterococcus hirae isolated from the rumen of Bos primigenius","volume":"30","author":"S Arokiyaraj","year":"2014","journal-title":"World J Microbiol Biotechnol"},{"issue":"1","key":"pone.0357625.ref060","first-page":"37","article-title":"Selection of bacteriocin producer strains of 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