{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T18:14:37Z","timestamp":1788459277045,"version":"build-2803163510"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To address the safety risk of mistaking tap water or purified water for acidic electrolyzed oxidizing water (AEOW) during manual cleaning in central sterile supply departments (CSSDs), this study aimed to develop a low-cost, rapid identification device based on electrical conductivity differences to ensure the accuracy and safety of the disinfection process.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A lighthouse-shaped rapid identification device (LRID) was fabricated based on the significantly higher electrical conductivity of AEOW compared with tap water and purified water. Centered on an Arduino Nano microcontroller, the device integrates a conductivity sensor, a wireless charging module, and an LED indication system. It achieves automatic discrimination by detecting liquid electrical conductivity: the green LED remains illuminated for AEOW, while the red LED lights up for tap water or purified water. Identification accuracy, battery life, and waterproof performance were evaluated, and the satisfaction of CSSD technicians with the conventional pH test strip method and the LRID was compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The LRID achieved 100% identification accuracy (90\/90) for AEOW, tap water, and purified water collected from the CSSDs of 10 hospitals. The average battery life was 83.75\u2009\u00b1\u20091.27 h, supporting a weekly charging schedule. All devices functioned normally after continuous immersion in AEOW for 30 days, confirming reliable waterproof performance. Scores from 28 CSSD technicians showed that the LRID was significantly superior to the pH test strip method in identification accuracy, ease of operation, and promotability (P\u2009&lt;\u20090.001).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study successfully developed and validated a low-cost (approximately 20 USD), highly reliable, and easy-to-operate AEOW rapid identification device. The LRID effectively prevents disinfection failures caused by liquid misuse in CSSDs and holds significant potential for its application and promotion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0357672","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:28:56Z","timestamp":1788456536000},"page":"e0357672","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lighthouse-shaped rapid identification device for acidic electrolyzed oxidizing water: A low-cost detection method based on conductivity differences"],"prefix":"10.1371","volume":"21","author":[{"given":"Yuan","family":"Mao","sequence":"first","affiliation":[{"name":"363 Hospital","place":["Chengdu, China"],"department":["Central Sterile Supply Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital &Institute, Sichuan Cancer Center, Affiliated Cancer Hospital of University of Electronic Science and Technology of China","place":["Chengdu, China"],"department":["Central Sterile Supply Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1066-9660","authenticated-orcid":true,"given":"Qian","family":"Chen","sequence":"additional","affiliation":[{"name":"Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital &Institute, Sichuan Cancer Center, Affiliated Cancer Hospital of University of Electronic Science and Technology of China","place":["Chengdu, China"],"department":["Central Sterile Supply Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"issue":"1","key":"pone.0357672.ref001","first-page":"1","article-title":"Trends in acidic electrolyzed water (hypochlorous acid water) in terms of basic science and application","volume":"5","author":"H Kunimoto","year":"2010","journal-title":"Functional Water Foundation"},{"key":"pone.0357672.ref002","unstructured":"State Administration for Market Regulation of China. GB 28234-2020: Hygienic requirements for acidic electrolyzed water generator. Beijing: China Standard Press. 2020."},{"key":"pone.0357672.ref003","unstructured":"Ministry of Health Labour and Welfare of Japan. Report of the subcommittee on the revision of the composition standards of hypochlorite water. https:\/\/www.mhlw.go.jp\/shingi\/2007\/03\/dl\/s0320-7h.pdf"},{"issue":"4","key":"pone.0357672.ref004","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.jds.2019.09.007","article-title":"Influence of the acidity of electrolyzed water on the microhardness of inner layer dentin","volume":"14","author":"K-K Chen","year":"2019","journal-title":"J Dent Sci"},{"issue":"4","key":"pone.0357672.ref005","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.3390\/molecules27041222","article-title":"Electrolyzed Water and Its Pharmacological Activities: A Mini-Review","volume":"27","author":"B-K Chen","year":"2022","journal-title":"Molecules"},{"key":"pone.0357672.ref006","unstructured":"National Health Commission of China. WS 310.2: Central sterile supply department Part 2: Standard for operating procedure of cleaning, disinfection and sterilization. Beijing: China Standards Press. 2016."},{"issue":"9","key":"pone.0357672.ref007","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.4315\/0362-028X-71.9.1934","article-title":"Electrolyzed water and its application in the food industry","volume":"71","author":"D Hricova","year":"2008","journal-title":"J Food Prot"},{"issue":"1","key":"pone.0357672.ref008","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1186\/s13756-021-01037-z","article-title":"A narrative review on current duodenoscope reprocessing techniques and novel developments","volume":"10","author":"M Heuvelmans","year":"2021","journal-title":"Antimicrob Resist Infect Control"},{"issue":"06","key":"pone.0357672.ref009","doi-asserted-by":"crossref","first-page":"760","DOI":"10.4236\/fns.2012.36102","article-title":"Electrolyzed Oxidized Water (EOW): Non-Thermal Approach for Decontamination of Food Borne Microorganisms in Food Industry","volume":"03","author":"S Hati","year":"2012","journal-title":"FNS"},{"issue":"2","key":"pone.0357672.ref010","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s12393-021-09278-9","article-title":"Electrolyzed Oxidizing Water and Its Applications as Sanitation and Cleaning Agent","volume":"13","author":"A Iram","year":"2021","journal-title":"Food Eng Rev"},{"issue":"10","key":"pone.0357672.ref011","first-page":"1595","article-title":"Progress of research on application of acidic electrolyzed water and hypochlorous acid water in medical field","volume":"35","author":"Y Lyu","year":"2025","journal-title":"Chin J Nosocomiol"},{"key":"pone.0357672.ref012","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0168-1605(00)00405-0","article-title":"Efficacy of electrolyzed oxidizing (EO) and chemically modified water on different types of foodborne pathogens","volume":"61","author":"C Kim","year":"2000","journal-title":"Int J Food Microbiol"},{"key":"pone.0357672.ref013","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0168-1605(01)00622-5","article-title":"Antimicrobial effect of electrolyzed water for inactivating Campylobacter jejuni during poultry washing","volume":"72","author":"H Park","year":"2002","journal-title":"Int J Food Microbiol"},{"issue":"8","key":"pone.0357672.ref014","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.4315\/0362-028X-65.8.1276","article-title":"Effectiveness of electrolyzed water as a sanitizer for treating different surfaces","volume":"65","author":"H Park","year":"2002","journal-title":"J Food Prot"},{"issue":"6","key":"pone.0357672.ref015","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/j.1525-1594.2004.07293.x","article-title":"The bactericidal effects of electrolyzed oxidizing water on bacterial strains involved in hospital infections","volume":"28","author":"NV Vorobjeva","year":"2004","journal-title":"Artif Organs"},{"issue":"3","key":"pone.0357672.ref016","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.ijfoodmicro.2005.06.020","article-title":"Effects of electrolyzed oxidizing water on reducing Listeria monocytogenes contamination on seafood processing surfaces","volume":"106","author":"C Liu","year":"2006","journal-title":"Int J Food Microbiol"},{"issue":"8","key":"pone.0357672.ref017","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.4315\/0362-028X-69.8.1829","article-title":"Effects of electrolyzed oxidizing water treatment on reducing Vibrio parahaemolyticus and Vibrio vulnificus in raw oysters","volume":"69","author":"T Ren","year":"2006","journal-title":"J Food Prot"},{"key":"pone.0357672.ref018","doi-asserted-by":"crossref","first-page":"1328055","DOI":"10.3389\/fmicb.2023.1328055","article-title":"Slightly acidic electrolyzed water inhibits inflammation induced by membrane vesicles of Staphylococcus aureus","volume":"14","author":"Y Shimamura","year":"2024","journal-title":"Front Microbiol"},{"key":"pone.0357672.ref019","unstructured":"Strong Electrolyzed Water Council. Overview of electrolyzed hypochlorous acid water. https:\/\/www.env.go.jp\/council\/content\/i_08\/900431540.pdf. Accessed 2026 March 29."},{"issue":"1","key":"pone.0357672.ref020","first-page":"1","article-title":"Pursuing the safety of strongly acidic electrolyzed water","volume":"4","author":"H Komiyama","year":"2009","journal-title":"Functional Water Foundation"},{"issue":"12","key":"pone.0357672.ref021","doi-asserted-by":"crossref","first-page":"5908","DOI":"10.3390\/ijms26125908","article-title":"Combination of Slightly Acidic Electrolyzed Water and Hydrogel to Enhance Stability, Increase Antibacterial Efficacy, and Promote Infectious Wound Healing","volume":"26","author":"N Li","year":"2025","journal-title":"Int J Mol Sci"},{"issue":"12","key":"pone.0357672.ref022","first-page":"104","article-title":"Presurgical preparation of surgeons\u2019 hands with the products of electrolysis of a 3per cent solution of sodium chloride","volume":"41","author":"BA Nikitin","year":"1965","journal-title":"Khirurgiia (Mosk)"},{"issue":"10","key":"pone.0357672.ref023","first-page":"100","article-title":"From experience in the electrolytic decontamination of drinking water","volume":"32","author":"LA Kunina","year":"1967","journal-title":"Gig Sanit"},{"issue":"4","key":"pone.0357672.ref024","first-page":"16","article-title":"An effective method of electrolysis for decontamination of drinking water","volume":"33","author":"LA Sergunina","year":"1968","journal-title":"Gig Sanit"},{"key":"pone.0357672.ref025","unstructured":"Ministry of Health of the People\u2019s Republic of China. GB 28234-2011: Safety and sanitation standard for acidic electrolyzed oxidizing water generator. Beijing: China Standard Press. 2011."},{"issue":"1","key":"pone.0357672.ref026","doi-asserted-by":"crossref","first-page":"39","DOI":"10.3390\/foods12010039","article-title":"Effects of Slightly Acidic Electrolyzed Water on the Quality of Fresh-Cut Apple","volume":"12","author":"Q Gao","year":"2022","journal-title":"Foods"},{"issue":"23","key":"pone.0357672.ref027","doi-asserted-by":"crossref","DOI":"10.3390\/foods11233863","article-title":"Ultrasound-assisted slightly acidic electrolyzed water in aquatic product sterilization: A review","volume":"11","author":"C Zhang","year":"2022","journal-title":"Foods"},{"issue":"4","key":"pone.0357672.ref028","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.jhin.2008.01.024","article-title":"Ultrasonically nebulised electrolysed oxidising water: a promising new infection control programme for impressions, metals and gypsum casts used in dental hospitals","volume":"68","author":"G Wu","year":"2008","journal-title":"J Hosp Infect"},{"issue":"20","key":"pone.0357672.ref029","first-page":"4998","article-title":"Application of electrolyzed oxidizing water in central sterile supply department","volume":"23","author":"X Ma","year":"2013","journal-title":"Chin J Nosocomiol"},{"key":"pone.0357672.ref030","unstructured":"National Health Commission of China. WS 310.1: Central sterile supply department. Part 1: Management standard. Beijing: China Standards Press. 2016."},{"issue":"07","key":"pone.0357672.ref031","first-page":"151","article-title":"Investigation on the status quo of cleaning, disinfection and sterilization of reusable medical devices","volume":"30","author":"L Chen","year":"2024","journal-title":"Chin Med Device Inf"},{"issue":"39","key":"pone.0357672.ref032","doi-asserted-by":"crossref","first-page":"25209","DOI":"10.1021\/acsomega.0c03366","article-title":"Portable Nanohybrid Paper-Based Chemiresistive Sensor for Free Chlorine Detection","volume":"5","author":"Y-K Yen","year":"2020","journal-title":"ACS Omega"},{"key":"pone.0357672.ref033","doi-asserted-by":"crossref","first-page":"156193","DOI":"10.1016\/j.scitotenv.2022.156193","article-title":"Chlorination in the pandemic times: The current state of the art for monitoring chlorine residual in water and chlorine exposure in air","volume":"838","author":"T Li","year":"2022","journal-title":"Sci Total Environ"},{"issue":"2","key":"pone.0357672.ref034","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1039\/D3FB00212H","article-title":"Application of electrolysed water in post-harvest treatment of fruits and vegetables","volume":"2","author":"J Saxena","year":"2024","journal-title":"Sustainable Food Technol"},{"issue":"05","key":"pone.0357672.ref035","first-page":"119","article-title":"Physicochemical characteristics of acidic electrolyzed water with different treatments","author":"Z Zhu","year":"2008","journal-title":"Food Science and Technology"},{"key":"pone.0357672.ref036","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-642-21729-6_84","volume-title":"Measurement of available chlorine in electrolyzed water using electrical conductivity","author":"K Umimoto","year":"2011"},{"issue":"19","key":"pone.0357672.ref037","first-page":"232","article-title":"Effect of preparing condition on physicochemical property of slightly acidic electrolyzed functional water","volume":"28","author":"H Yatao","year":"2012","journal-title":"Transactions of the CSAE"},{"issue":"10","key":"pone.0357672.ref038","first-page":"138","article-title":"Effect of ion characteristics and storage conditions on physicochemical properties of acidic electrolyzed functional water","volume":"33","author":"K Li","year":"2012","journal-title":"Science and Technology of Food Industry"},{"key":"pone.0357672.ref039","unstructured":"China Electronics Standardization Institute. Public Consultation Notice: Draft Industry Standard for Water Quality Specification of Reclaimed Water for Electronic Ultrapure Water. https:\/\/www.qianwen.com\/chat\/b301e542c07d49128968bce6d37de142. Accessed 2026 August 6."},{"key":"pone.0357672.ref040","unstructured":"Pharmaceutical Affairs and Food Sanitation Council of the Ministry of Health LaW. Summary of study results on designation of food additives and revision of usage standards. https:\/\/www.mhlw.go.jp\/houdou\/0111\/h1106-2.html"},{"issue":"1","key":"pone.0357672.ref041","doi-asserted-by":"crossref","first-page":"117","DOI":"10.14744\/eej.2020.52523","article-title":"Influence of Lignocaine Hydrochloride with Adrenaline on Free Active Chlorine Content of Sodium Hypochlorite Solution Admixed in Various Proportions","volume":"6","author":"I Gurucharan","year":"2021","journal-title":"Eur Endod J"},{"issue":"8","key":"pone.0357672.ref042","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0289534","article-title":"Elucidation of composition of chlorine compounds in acidic sodium chlorite solution using ion chromatography","volume":"18","author":"A Kishimoto","year":"2023","journal-title":"PLoS One"},{"issue":"07","key":"pone.0357672.ref043","first-page":"11","article-title":"Study on the physicochemical properties and decolorization ability of acidic electrolyzed water","volume":"44","author":"BY Li","year":"2024","journal-title":"Shanxi Chemical Industry"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0357672","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:29:02Z","timestamp":1788456542000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0357672"}},"subtitle":[],"editor":[{"given":"Karthik","family":"Kannan","sequence":"first","affiliation":[{"name":"National Chung Cheng University College of Engineering","place":["TAIWAN"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":43,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0357672","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]}}}