{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:49:22Z","timestamp":1792788562935,"version":"4.5.0"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Irish Health Research Board","award":["EIA-2017-013"],"award-info":[{"award-number":["EIA-2017-013"]}],"id":[{"id":"https:\/\/ror.org\/003hb2249","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Maynooth University Teaching Fellowship","id":[{"id":"https:\/\/ror.org\/048nfjm95","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Lonsdale Institute for Human Health Research"},{"name":"Celvia and the Lonsdale Institute for Human Health Research"},{"name":"European Union\u2019s Horizon Europe Excellent Science programme under the Marie Sk\u0142odowska-Curie Actions Grant Agreement","award":["101126640"],"award-info":[{"award-number":["101126640"]}]},{"name":"C\u00daRAM, Science Foundation Ireland Research Centre for Medical Devices","award":["13\/RC\/2073_P2"],"award-info":[{"award-number":["13\/RC\/2073_P2"]}],"id":[{"id":"https:\/\/ror.org\/0271asj38","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background and Aims<\/jats:title>\n                    <jats:p>N-glycans modulate glycoprotein structure and function and are altered during chronic inflammation. We sought to define the extent of serum and IgG N-glycan disruption in patients with decompensated liver cirrhosis from alcohol-related liver disease (ALD), primary sclerosing cholangitis (PSC), and ALD-related hepatocellular carcinoma (HCC). Finally, we aimed to examine whether serum and IgG glycosylation is associated with changes in Model for End-stage Liver Disease-Sodium (MELD-Na) scores, a clinical marker used to prioritise liver transplantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serum samples were obtained from patients with ALD (n\u2009=\u200917), PSC (n\u2009=\u20097), ALD-related HCC (n\u2009=\u20094), and healthy controls (n\u2009=\u200910). N-glycans were released, fluorescently labelled, and profiled by hydrophilic interaction ultra performance liquid chromatography (HILIC-UPLC). Chromatograms were integrated into 46 and 23 glycan peaks for serum and IgG respectively. These peaks and their associated glycosylation traits were statistically compared with healthy controls using age- and sex-adjusted linear regression models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In serum, decompensated cirrhosis shows statistically significant shifts toward less complex, agalactosylated and asialylated biantennary glycans, accompanied by significant losses of highly branched, galactosylated and sialylated structures. IgG mirrored this pattern, which is characteristic of a pro-inflammatory signature, with increased agalactosylation and bisected glycan levels, along with reduced levels of digalactosylated and sialylated species. N-glycan profiles showed significant associations with MELD-Na scores, indicating that inflammatory processes in decompensated liver cirrhosis continue to reshape serum glycoproteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Decompensated liver cirrhosis shows profound remodelling of serum and IgG N-glycans. These data establish a reference framework for terminal glycomic disruption in liver disease and highlight the potential value of incorporating glycosylation analysis into broader assessments of liver disease progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0357709","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:27:26Z","timestamp":1789493246000},"page":"e0357709","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dysregulation of the serum and IgG N-glycome in decompensated cirrhosis and its association with Model for End-Stage Liver Disease-Sodium (MELD-Na)"],"prefix":"10.1371","volume":"21","author":[{"given":"Andreea","family":"Cislaru","sequence":"first","affiliation":[{"name":"Maynooth University","place":["Maynooth, Co. Kildare, Ireland"],"department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3964-5425","authenticated-orcid":true,"given":"Emre","family":"Yagmur","sequence":"additional","affiliation":[{"name":"Maynooth University","place":["Maynooth, Co. Kildare, Ireland"],"department":["Department of Chemistry"]},{"name":"Maynooth University","place":["Maynooth, Co. Kildare, Ireland"],"department":["Kathleen Lonsdale Institute for Human Health Research"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Bannon","sequence":"additional","affiliation":[{"name":"Maynooth University","place":["Maynooth, Co. Kildare, Ireland"],"department":["Department of Chemistry"]},{"name":"Maynooth University","place":["Maynooth, Co. Kildare, Ireland"],"department":["Kathleen Lonsdale Institute for Human Health Research"]},{"name":"Maynooth University","place":["Maynooth, Co. Kildare, Ireland"],"department":["Department of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinead","family":"Cremen","sequence":"additional","affiliation":[{"name":"University College Dublin","place":["Belfield, Dublin, Ireland"],"department":["School of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom K.","family":"Gallagher","sequence":"additional","affiliation":[{"name":"St. Vincent\u2019s University Hospital","place":["Elm Park, Dublin, Ireland"],"department":["Department of Hepatopancreaticobiliary and Transplant Surgery"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo","family":"Sarti","sequence":"additional","affiliation":[{"name":"Royal College of Surgeons in Ireland, University of Medicine and Health Sciences","place":["Saint Stephen\u2019s Green, Dublin, Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Maynooth University","place":["Maynooth, Co. 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