{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:08:55Z","timestamp":1789150135522,"version":"build-2803163510"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160235"],"award-info":[{"award-number":["32160235"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Guangxi Natural Science Foundation","award":["2024GXNSFAA010328"],"award-info":[{"award-number":["2024GXNSFAA010328"]}]},{"name":"the Project of Improving the Basic Scientific Research Ability of Young and Middle\u2010Aged Teachers in Guangxi Universities","award":["2023KY0856"],"award-info":[{"award-number":["2023KY0856"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Diagnosis of Alzheimer\u2019s disease (AD) relies on invasive cerebrospinal fluid analysis or costly neuroimaging, underscoring the need for minimally invasive blood-based biomarkers for early detection. Exosomes, promising biomarker carriers, are unexplored for ultra-short RNA species like abortive transcripts (ATs). We investigated whether 8-nucleotide ATs are selectively packaged into exosomes to reflect brain transcriptional dysregulation in AD. Using a transgenic AD mouse model and A\u03b2-stimulated BV2 microglia coupled with Base-Stacking Hybridization Assisted Ligation (BSHAL), we detected significant dysregulation of ATs from AD-relevant genes (\n                    <jats:italic>Nefl<\/jats:italic>\n                    ,\n                    <jats:italic>Bace1<\/jats:italic>\n                    ,\n                    <jats:italic>Tyrobp<\/jats:italic>\n                    ,\n                    <jats:italic>Ccl2<\/jats:italic>\n                    ,\n                    <jats:italic>Pf4<\/jats:italic>\n                    ) in brain tissue; specifically,\n                    <jats:italic>Bace1<\/jats:italic>\n                    ,\n                    <jats:italic>Tyrobp<\/jats:italic>\n                    , and\n                    <jats:italic>Pf4<\/jats:italic>\n                    ATs showed robust increases (2.21-fold to 17.50-fold). These dysregulated AT signatures were mirrored in peripheral blood exosomes:\n                    <jats:italic>Bace1<\/jats:italic>\n                    ,\n                    <jats:italic>Tyrobp<\/jats:italic>\n                    , and\n                    <jats:italic>Pf4<\/jats:italic>\n                    ATs increased 10.58-, 38.72-, and 11.63-fold, respectively. A\u03b2-activated microglia demonstrated exosome-specific enrichment of ATs, particularly\n                    <jats:italic>Pf4<\/jats:italic>\n                    ATs (~3510-fold increase), confirming active exosomal packaging. Exosomal ATs confer cell-type specificity for central nervous system pathology, lipid bilayer-enhanced stability, and sensitivity to transcriptional changes preceding pathological aggregation. Our findings, obtained in a transgenic mouse model and an in vitro microglial system, establish exosomal ATs as a novel class of blood-based biomarkers with potential for early AD diagnosis, pending validation in human cohorts.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0357752","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:31:48Z","timestamp":1789147908000},"page":"e0357752","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Abortive transcripts in exosomes - A potential biomarker for Alzheimer\u2019s disease"],"prefix":"10.1371","volume":"21","author":[{"given":"Haizhu","family":"Wu","sequence":"first","affiliation":[{"name":"Guilin Tourism University, School of Food and Health","place":["Guilin, China"]},{"name":"Guilin Tourism University, Guangxi Engineering Research Center for Large-Scale Preparation & Nutrients and Hygiene of Guangxi 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