{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:03:27Z","timestamp":1790877807642,"version":"4.1.0"},"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018726","name":"Torbat Heydariyeh University of Medical Sciences","doi-asserted-by":"publisher","award":["400000078"],"award-info":[{"award-number":["400000078"]}],"id":[{"id":"10.13039\/100018726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Non-alcoholic fatty liver disease (NAFLD) is the most prevalent chronic liver disorder worldwide. This study aims to investigate the effects of crocin on lipid profile, hepatic lipid accumulation, and antioxidant status in a rat model of non-alcoholic fatty liver induced by a high-fat diet.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>Forty-two male Wistar rats were divided into six groups, each containing seven rats. Fatty liver was induced by feeding a high-fat diet for eight weeks. Subsequently, the treatment groups received intraperitoneal crocin for four weeks. The groups were then evaluated for changes in serum lipid profiles, liver function enzymes in serum, oxidative stress biomarkers and histopathological alterations in liver tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>High-fat diet feeding resulted in significant hepatic lipid accumulation, elevated serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase levels, increased hepatic malondialdehyde and nitrite concentrations, and impaired antioxidant enzyme activities. Histological analysis demonstrated hepatocellular steatosis, inflammatory infiltration, and hepatic structural alterations. Crocin treatment significantly attenuated lipid accumulation, normalized serum liver enzyme levels, reduced oxidative stress markers, and enhanced antioxidant enzyme activities. Histopathological evaluation further confirmed marked improvement in liver tissue morphology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The results of the present study demonstrate that Crocin exerts protective effects against NAFLD, highlighting its potential as a therapeutic agent for diet-induced fatty liver disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0357768","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:26:18Z","timestamp":1790875578000},"page":"e0357768","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Protective effects of Crocin on hepatic steatosis, oxidative stress, and lipid profile in an animal model of non-alcoholic fatty liver disease"],"prefix":"10.1371","volume":"21","author":[{"given":"Hossain","family":"Rezayee","sequence":"first","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Student Research Committee"]},{"name":"Khatam Al-Nabieen University","place":["Kabul, Afghanistan"],"department":["Department of Paracilinic, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ali","family":"Fahmidehkar","sequence":"additional","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Department of Clinical Biochemistry, School of Medicine"]},{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Research Center of Advanced Technologies in Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farimah","family":"Beheshti","sequence":"additional","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Departments of Physiology, School of Medicine"]},{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Neuroscience Research Center"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Ghoryani","sequence":"additional","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Department of Laboratory Sciences, School of Paramedical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaser","family":"Yousefpoor","sequence":"additional","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Research Center of Advanced Technologies in Medicine"]},{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Department of Medical Biotechnology, School of Paramedical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawood","family":"Hossaini","sequence":"additional","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Student Research Committee"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4031-4181","authenticated-orcid":true,"given":"Davod","family":"Ilbeigi","sequence":"additional","affiliation":[{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Department of Clinical Biochemistry, School of Medicine"]},{"name":"Torbat Heydariyeh University of Medical Sciences","place":["Torbat Heydariyeh, Iran"],"department":["Neuroscience Research Center"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"issue":"18","key":"pone.0357768.ref001","doi-asserted-by":"crossref","first-page":"3313","DOI":"10.1007\/s00018-018-2860-6","article-title":"Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease","volume":"75","author":"DH Ipsen","year":"2018","journal-title":"Cell Mol Life Sci"},{"issue":"10290","key":"pone.0357768.ref002","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1016\/S0140-6736(20)32511-3","article-title":"Non-alcoholic fatty liver disease","volume":"397","author":"EE Powell","year":"2021","journal-title":"Lancet"},{"key":"pone.0357768.ref003","unstructured":"World Health Organization. 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