{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:05:01Z","timestamp":1789754701364,"version":"4.0.1"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004351","name":"Sultan Qaboos University","doi-asserted-by":"publisher","award":["CL\/SQU-SHOU\/AGR\/24\/01, IG\/AGR\/FISH\/24\/01"],"award-info":[{"award-number":["CL\/SQU-SHOU\/AGR\/24\/01, IG\/AGR\/FISH\/24\/01"]}],"id":[{"id":"10.13039\/501100004351","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004787","name":"The Research Council","doi-asserted-by":"publisher","award":["RC\/GRG\/AGR\/MS&\/24\/001"],"award-info":[{"award-number":["RC\/GRG\/AGR\/MS&\/24\/001"]}],"id":[{"id":"10.13039\/501100004787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Biofouling remains a major challenge for submerged materials in marine and industrial environments, reducing performance and increasing maintenance costs. In this study, zinc oxide nanorod (ZnO NR) coatings with dual-scale surface roughness were fabricated on three distinct 3D micropatterned surfaces, designated as D1, D2, and D3. The antifouling performance of the engineered surfaces was evaluated against the bacterium\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and the diatom\n                    <jats:italic>Amphora<\/jats:italic>\n                    sp. under laboratory flow conditions, while acute toxicity was assessed using larvae of\n                    <jats:italic>Litopenaeus vannamei<\/jats:italic>\n                    . Fourier transform infrared (FTIR) spectroscopy confirmed the presence of Zn\u2013O stretching vibrations, particularly on the ZnO NR-coated D2 and D3 surfaces. Water contact angle measurements showed that the ZnO NR-coated surfaces were superhydrophobic (\u2248150\u2013165\u00b0) because of their hierarchical micro\/nanostructures, whereas the non-coated surfaces exhibited moderate wettability (\u224880\u201390\u00b0). ZnO coating reduced bacterial attachment by 60.3%, 48.8%, and 5.8% on the D1, D2, and D3 surfaces, respectively. Diatom coverage was reduced by 9.9%, 72.9%, and 71.8% on the corresponding ZnO-coated surfaces. The enhanced antifouling performance was associated with the combined effects of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    ions, ROS generation, surface wettability, and micro\/nanotopography. The non-coated D1 and D2 surfaces, together with the ZnO-coated D1 surface, exhibited no significant toxicity toward shrimp larvae. In contrast, the ZnO-coated D3 surface exhibited relatively low antifouling performance and higher larval toxicity. Among the tested surfaces, the ZnO-coated D1 micropattern demonstrated the best overall performance by combining high antifouling efficacy with low toxicity. Post-characterization SEM analysis confirmed that the ZnO nanorod coatings remained structurally intact after biological testing, indicating good coating stability under the experimental conditions. These findings demonstrate that combining ZnO nanorod coatings with micropatterned surface topography is a promising strategy for antifouling applications. However, further long-term field evaluations and comprehensive ecotoxicological studies are required to assess the environmental performance of these coatings.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0357826","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T17:21:50Z","timestamp":1789752110000},"page":"e0357826","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antifouling properties of ZnO nanorods coating on micropatterned polymers"],"prefix":"10.1371","volume":"21","author":[{"given":"Azhar","family":"Al-Busaidi","sequence":"first","affiliation":[{"name":"Sultan Qaboos University","place":["Muscat, Oman"],"department":["Department of Marine Science and Fisheries, College of Agriculture and Marine 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Nir","year":"2016","journal-title":"Curr Opin Biotechnol"},{"key":"pone.0357826.ref007","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1146\/annurev-matsci-070511-155012","article-title":"Bio-inspired antifouling strategies","volume":"42","author":"CM Kirschner","year":"2012","journal-title":"Annu Rev Mater Res"},{"issue":"4","key":"pone.0357826.ref008","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/S1369-7021(10)70058-4","article-title":"Non-toxic antifouling strategies","volume":"13","author":"CM Magin","year":"2010","journal-title":"Mater Today"},{"issue":"6","key":"pone.0357826.ref009","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.3390\/microorganisms11061568","article-title":"Development of antifouling strategies for marine applications","volume":"11","author":"MJ Romeu","year":"2023","journal-title":"Microorganisms"},{"issue":"1967","key":"pone.0357826.ref010","first-page":"2381","article-title":"Biofouling: lessons from 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Scardino","year":"2011","journal-title":"Biofouling"},{"key":"pone.0357826.ref014","first-page":"1","article-title":"Marine biofouling: biology, impacts, and new technologies","volume":"113","author":"SP Neill","year":"2016","journal-title":"Mar Pollut Bull"},{"key":"pone.0357826.ref015","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1080\/08927010500484854","article-title":"Engineered antifouling microtopographies--correlating wettability with cell attachment","volume":"22","author":"ML Carman","year":"2006","journal-title":"Biofouling"},{"issue":"1","key":"pone.0357826.ref016","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0022-0981(00)00210-0","article-title":"Analysis of behavioural rejection of micro-textured surfaces and implications for recruitment by the barnacle Balanus improvisus","volume":"251","author":"K Berntsson","year":"2000","journal-title":"J Exp Mar Biol 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Chem"},{"issue":"4","key":"pone.0357826.ref020","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1080\/08927014.2016.1146256","article-title":"Self-decontaminating photocatalytic zinc oxide nanorod coatings for prevention of marine microfouling: a mesocosm study","volume":"32","author":"P Sathe","year":"2016","journal-title":"Biofouling"},{"key":"pone.0357826.ref021","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.cattod.2016.09.014","article-title":"Defect engineered visible light active ZnO nanorods for photocatalytic treatment of water","volume":"284","author":"T Bora","year":"2017","journal-title":"Catal Today"},{"issue":"7","key":"pone.0357826.ref022","doi-asserted-by":"crossref","first-page":"4020","DOI":"10.1021\/la104825u","article-title":"Size-dependent bacterial growth inhibition and mechanism of antibacterial activity of zinc oxide nanoparticles","volume":"27","author":"KR Raghupathi","year":"2011","journal-title":"Langmuir"},{"issue":"24","key":"pone.0357826.ref023","doi-asserted-by":"crossref","first-page":"245705","DOI":"10.1088\/0957-4484\/19\/24\/245705","article-title":"Sonochemical coating of silver nanoparticles on textile fabrics (nylon, polyester and cotton) and their antibacterial activity","volume":"19","author":"I Perelshtein","year":"2008","journal-title":"Nanotechnology"},{"issue":"16","key":"pone.0357826.ref024","doi-asserted-by":"crossref","first-page":"13397","DOI":"10.1021\/acsami.8b03681","article-title":"Nano-protrusive gold nanoparticle-hybridized polymer thin film as a sensitive, multipatternable, and antifouling biosensor platform","volume":"10","author":"J-H Lee","year":"2018","journal-title":"ACS Appl Mater Interfaces"},{"issue":"1","key":"pone.0357826.ref025","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s40824-023-00344-3","article-title":"Instant formation of horizontally ordered nanofibrous hydrogel films 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Muthukrishnan","year":"2017","journal-title":"Mar Environ Res"},{"key":"pone.0357826.ref031","doi-asserted-by":"crossref","first-page":"130357","DOI":"10.1016\/j.colsurfa.2022.130357","article-title":"Anti-biofouling properties of TiO2 coating with coupled effect of photocatalysis and microstructure","volume":"656","author":"P Yi","year":"2023","journal-title":"Colloids Surf A: Physicochem Eng Asp"},{"key":"pone.0357826.ref032","doi-asserted-by":"crossref","first-page":"10815","DOI":"10.1002\/9780470027318.a5606","article-title":"Interpretation of infrared spectra, a practical approach.","volume-title":"Encyclopedia of analytical chemistry","author":"J. Coates","year":"2000"},{"key":"pone.0357826.ref033","doi-asserted-by":"crossref","DOI":"10.1201\/b10777","volume-title":"Fundamentals of Fourier transform infrared spectroscopy","author":"BC Smith","year":"2011","edition":"2"},{"key":"pone.0357826.ref034","doi-asserted-by":"crossref","DOI":"10.1002\/0470011149","volume-title":"Infrared spectroscopy: fundamentals and applications","author":"B Stuart","year":"2004"},{"issue":"1","key":"pone.0357826.ref035","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/08927014.2018.1557637","article-title":"Evaluating the effect of antiscalants on membrane biofouling using FTIR and multivariate analysis","volume":"35","author":"MY Ashfaq","year":"2019","journal-title":"Biofouling"},{"key":"pone.0357826.ref036","first-page":"90","article-title":"Polyurethane\/ZnO nanocomposite coatings: structure and properties","volume":"132","author":"E Ghasemi","year":"2019","journal-title":"Prog Org Coat"},{"issue":"5","key":"pone.0357826.ref037","first-page":"1303","article-title":"FTIR and structural characterization of ZnO\u2013PU nanocomposite coatings","volume":"18","author":"MM Rahman","year":"2021","journal-title":"J Coat Technol Res"},{"key":"pone.0357826.ref038","first-page":"144532","article-title":"Antibacterial polyurethane coatings containing ZnO nanoparticles","volume":"505","author":"NA Al-Harbi","year":"2020","journal-title":"Appl Surf Sci"},{"issue":"5771","key":"pone.0357826.ref039","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1126\/science.1124005","article-title":"Piezoelectric nanogenerators based on zinc oxide nanowire arrays","volume":"312","author":"ZL Wang","year":"2006","journal-title":"Science"},{"issue":"2","key":"pone.0357826.ref040","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/10408436.2021.1886041","article-title":"Synthesis and applications of ZnO nanostructures (ZONSs): a review","volume":"47","author":"MT Noman","year":"2022","journal-title":"Crit Rev Solid State Mater Sci"},{"issue":"8","key":"pone.0357826.ref041","first-page":"1853","article-title":"Structure and properties of polyurethane foams modified with bio-based polyols","volume":"13","author":"M Kura\u0144ska","year":"2020","journal-title":"Materials"},{"key":"pone.0357826.ref042","volume-title":"Paint and coating testing manual","author":"JV Koleske","year":"2015"},{"key":"pone.0357826.ref043","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1039\/tf9444000546","article-title":"Wettability of porous surfaces","volume":"40","author":"ABD Cassie","year":"1944","journal-title":"Trans Faraday Soc"},{"issue":"1","key":"pone.0357826.ref044","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3390\/polym16010017","article-title":"Influence of post-processing on the degree of conversion and mechanical properties of 3D-printed polyurethane aligners","volume":"16","author":"L \u0160imunovi\u0107","year":"2023","journal-title":"Polymers (Basel)"},{"issue":"4","key":"pone.0357826.ref045","doi-asserted-by":"crossref","DOI":"10.3390\/polym15040924","article-title":"Impact of the chemical structure of Photoreactive urethane (meth)acrylates with various (meth)acrylate groups and built-In Diels\u2013Alder reaction adducts on the UV-curing process and self-healing properties","volume":"15","author":"P Bednarczyk","year":"2023","journal-title":"Polymers"},{"issue":"18","key":"pone.0357826.ref046","doi-asserted-by":"crossref","DOI":"10.1002\/app.53817","article-title":"Castor oil\u2010based UV\u2010curable polyurethane acrylate resins for digital light processing (DLP) 3D printing technology","volume":"140","author":"H Bhanushali","year":"2023","journal-title":"J Appl Polym Sci"},{"issue":"2","key":"pone.0357826.ref047","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s00289-014-1268-1","article-title":"Synthesis and investigation of thermal and mechanical properties of in situ prepared biocompatible Fe3O4\/polyurethane elastomer nanocomposites","volume":"72","author":"A Mohammadi","year":"2014","journal-title":"Polym Bull"},{"key":"pone.0357826.ref048","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1016\/j.ijbiomac.2020.08.096","article-title":"ZnO nanorod-chitosan composite coatings with enhanced antifouling properties","volume":"162","author":"MA Al-Belushi","year":"2020","journal-title":"Int J Biol Macromol"},{"key":"pone.0357826.ref049","first-page":"125387","article-title":"Effect of micro\/nano hierarchical structures on wettability and antifouling performance of ZnO-based coatings","volume":"385","author":"L Zhang","year":"2020","journal-title":"Surf Coat Technol"},{"key":"pone.0357826.ref050","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/1559-4106-8-30","article-title":"The interaction of marine fouling organisms with topography of varied scale and geometry: a review","volume":"8","author":"FWY Myan","year":"2023","journal-title":"Biointerphases"},{"key":"pone.0357826.ref051","first-page":"111628","article-title":"Effects of micropattern geometry on bacterial adhesion: insights and mechanisms","volume":"201","author":"X Li","year":"2021","journal-title":"Colloids Surf B: Biointerfaces"},{"key":"pone.0357826.ref052","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3389\/fmicb.2019.00191","article-title":"Micro- and nanotopography sensitive bacterial attachment mechanisms: a review","volume":"10","author":"Y Cheng","year":"2019","journal-title":"Front Microbiol"},{"key":"pone.0357826.ref053","volume-title":"Brock biology of microorganisms","author":"MT Madigan","year":"2021","edition":"16"},{"key":"pone.0357826.ref054","first-page":"100253","article-title":"Patterned surface effects on bacterial attachment and biofilm formation: a review of design strategies for antifouling microtopographies","volume":"15","author":"J Kim","year":"2022","journal-title":"Mater Today Adv"},{"issue":"1","key":"pone.0357826.ref055","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3390\/toxics12010048","article-title":"Ecological risks of zinc oxide nanoparticles for early life stages of obscure puffer (Takifugu obscurus)","volume":"12","author":"S Tang","year":"2024","journal-title":"Toxics"},{"issue":"6","key":"pone.0357826.ref056","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1093\/icb\/42.6.1116","article-title":"The influence of surface wettability on the adhesion strength of settled spores of the green alga enteromorpha and the diatom amphora","volume":"42","author":"JA Finlay","year":"2002","journal-title":"Integrat Comparat Biol"},{"key":"pone.0357826.ref057","first-page":"526","article-title":"Influence of surface wettability and flow on diatom attachment: mechanistic insights from controlled assays","volume":"632","author":"S Tang","year":"2024","journal-title":"J Colloid Interface Sci"},{"issue":"6","key":"pone.0357826.ref058","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1080\/08927014.2013.800192","article-title":"A novel approach to determine the efficacy of patterned surfaces for biofouling control in relation to its microfluidic environment","volume":"29","author":"P Halder","year":"2013","journal-title":"Biofouling"},{"issue":"1","key":"pone.0357826.ref059","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/08927014.2010.536837","article-title":"Mini review: biomimetic models and bioinspired surfaces for fouling control","volume":"27","author":"AJ Scardino","year":"2011","journal-title":"Biofouling"},{"issue":"1","key":"pone.0357826.ref060","doi-asserted-by":"crossref","first-page":"104","DOI":"10.62810\/jnsr.v3i1.103","article-title":"Environmental fate and toxicity of zinc oxide nanoparticles in aquatic ecosystems: a comprehensive review","volume":"3","author":"N Amin","year":"2025","journal-title":"JNSR"},{"issue":"1","key":"pone.0357826.ref061","first-page":"159","article-title":"Salinity and toxicity of zinc oxide nanoparticles in aquatic systems: a review study","volume":"15","author":"SM Imran","year":"2025","journal-title":"J Adv Vet Res"},{"key":"pone.0357826.ref062","doi-asserted-by":"crossref","first-page":"119396","DOI":"10.1016\/j.jenvman.2023.119396","article-title":"Ecotoxicological effects of zinc oxide nanoparticles (ZnO-NPs) on aquatic organisms: current research and emerging trends","volume":"349","author":"ER Bordin","year":"2024","journal-title":"J Environ Manag"},{"key":"pone.0357826.ref063","doi-asserted-by":"crossref","first-page":"133717","DOI":"10.1016\/j.scitotenv.2019.133717","article-title":"The effects of ZnO nanostructures of different morphology on bioenergetics and stress response biomarkers of the blue mussels Mytilus edulis","volume":"694","author":"HI Falfushynska","year":"2019","journal-title":"Sci Total 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Myint","year":"2026","journal-title":"Opt Mater"},{"key":"pone.0357826.ref067","first-page":"153031","article-title":"Reactive oxygen species generation and antibacterial mechanisms of ZnO-based photocatalysts under visible light","volume":"589","author":"C Zhou","year":"2022","journal-title":"Appl Surf Sci"},{"issue":"3","key":"pone.0357826.ref068","first-page":"219","article-title":"Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism","volume":"7","author":"A Sirelkhatim","year":"2015","journal-title":"Nanomicro Lett"},{"key":"pone.0357826.ref069","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1016\/j.rser.2017.08.020","article-title":"A review of ZnO nanoparticles as solar photocatalysts: synthesis, mechanisms and applications","volume":"81","author":"CB Ong","year":"2018","journal-title":"Renew Sustain Energy Rev"}],"container-title":["PLOS 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