{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:50:29Z","timestamp":1792788629397,"version":"4.5.0"},"reference-count":34,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Higher Education, Science and Innovations of the Republic of Uzbekistan","award":["FZ-2019121924"],"award-info":[{"award-number":["FZ-2019121924"]}],"id":[{"id":"https:\/\/ror.org\/050ge8w06","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Bioversity International\/UNEP-GEF project","award":["In situ\/On farm conservation and use of agrobiodiversity (fruit crops and wild fruit species) in Central Asia"],"award-info":[{"award-number":["In situ\/On farm conservation and use of agrobiodiversity (fruit crops and wild fruit species) in Central Asia"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Apple (\n                    <jats:italic>Malus domestica Borkh<\/jats:italic>\n                    .) is one of the most economically important fruit crops worldwide; however, its production is severely constrained by major diseases, including fire blight, powdery mildew, and apple scab. Breeding disease-resistant cultivars represents a sustainable alternative to chemical control, particularly through the effective utilization of local germplasm resources from Central Asia. This study aimed to evaluate the presence and distribution of resistance-associated alleles in local apple varieties from Uzbekistan using polymorphic DNA markers. A collection of local apple accessions was screened to identify markers linked to resistance against fire blight, powdery mildew, and apple scab. The analysis revealed substantial genetic variation in resistance gene combinations among the studied varieties. The fire blight-associated marker AE10-375 was detected in 79.8% of the accessions. For powdery mildew resistance, 75.2% of the varieties carried resistance alleles corresponding to both Pl1 and Pl2 genes. Screening for apple scab resistance demonstrated that Vfa2\n                    <jats:italic>, Vfa1<\/jats:italic>\n                    , and Rvi6 were the most prevalent genes, with Vfa2 detected in 95.4% of the accessions. Regional analysis indicated that accessions from Karakalpakstan exhibited the highest proportion of genotypes harboring markers associated with resistance to multiple diseases. Six local varieties\u2014Atlas olma, Turkish, Xuboni, Krasniy jeleznyak, Shoyi olma, and Besh barmoq\u2014were identified as carrying resistance-associated markers for all three diseases. These findings demonstrate that local apple germplasm from Uzbekistan represents a valuable genetic resource for resistance to economically important diseases. The identified genotypes provide promising donor material for breeding programs aimed at developing cultivars with durable, broad-spectrum resistance while reducing reliance on chemical control strategies.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0357988","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T17:33:16Z","timestamp":1789493596000},"page":"e0357988","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Marker-assisted screening of resistance to fire blight, powdery mildew, and apple scab in local apple varieties from Uzbekistan"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1996-629X","authenticated-orcid":true,"given":"Abdushukur I.","family":"Rakhmatullaev","sequence":"first","affiliation":[{"name":"Center for Advanced Technologies","place":["Almazar, Tashkent, Uzbekistan"],"department":["Biotechnology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhabbat K.","family":"Turdieva","sequence":"additional","affiliation":[{"name":"office for Central Asia, Alliance of Bioversity International and CIAT","place":["Tashkent, Uzbekistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marguba M.","family":"Rejapova","sequence":"additional","affiliation":[{"name":"Center for Advanced Technologies","place":["Almazar, Tashkent, Uzbekistan"],"department":["Biotechnology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abrorjon A.","family":"Abdurakhimov","sequence":"additional","affiliation":[{"name":"Center for Advanced Technologies","place":["Almazar, Tashkent, Uzbekistan"],"department":["Biotechnology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisher A.","family":"Abdullaev","sequence":"additional","affiliation":[{"name":"Center for Advanced Technologies","place":["Almazar, Tashkent, Uzbekistan"],"department":["Biotechnology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"pone.0357988.ref001","first-page":"1","article-title":"Apples.","volume-title":"Jain SM, Priyadarshan PM, editors. 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State of the art of integrated crop management and organic systems in Europe, with particular reference to pest management","author":"S Tresnik","year":"2007"},{"issue":"2","key":"pone.0357988.ref007","doi-asserted-by":"crossref","first-page":"527","DOI":"10.3390\/ijms22020527","article-title":"Apple autotetraploids with enhanced resistance to apple scab (Venturia inaequalis) due to genome duplication-phenotypic and genetic evaluation","volume":"22","author":"M Podwyszy\u0144ska","year":"2021","journal-title":"Int J Mol Sci"},{"issue":"1","key":"pone.0357988.ref008","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1111\/j.1365-2338.1979.tb02223.x","article-title":"Recent progress in breeding for fireblight resistance in apples and pears in North America1","volume":"9","author":"HS Aldwinckle","year":"1979","journal-title":"EPPO Bulletin"},{"key":"pone.0357988.ref009","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1079\/9780851995922.0031","article-title":"Genetic 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J"},{"issue":"12","key":"pone.0357988.ref019","first-page":"75","article-title":"Effectiveness of modern fungicides against powdery mildew found in intensive apple orchards","volume":"7","author":"UZ Abdishukurovich","year":"2021","journal-title":"JournalNX"},{"issue":"9","key":"pone.0357988.ref020","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1094\/PDIS-12-15-1416-RE","article-title":"Integrated control of apple scab and powdery mildew in an organic apple orchard by combining potassium carbonates with wettable sulfur, pruning, and cultivar susceptibility","volume":"100","author":"IJ Holb","year":"2016","journal-title":"Plant Dis"},{"issue":"6","key":"pone.0357988.ref021","doi-asserted-by":"crossref","first-page":"474","DOI":"10.21273\/HORTSCI.5.6.474","article-title":"Rating of pear cultivars for resistance to fire blight","volume":"5","author":"WA Oitto","year":"1970","journal-title":"horts"},{"issue":"8","key":"pone.0357988.ref022","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1002\/ps.811","article-title":"Screening extracts of Achyranthes japonica and Rumex crispus for activity against various plant pathogenic fungi and control of powdery mildew","volume":"60","author":"J-C Kim","year":"2004","journal-title":"Pest Manag Sci"},{"key":"pone.0357988.ref023","first-page":"229","article-title":"Determination of most appropriate fungicide application time for controlling of apple scab in the region of Gjilan, Kosovo","author":"E Rexhepi","year":"2017","journal-title":"Albanian J Agricul Sci"},{"issue":"6","key":"pone.0357988.ref024","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1111\/j.1439-0523.1995.tb00850.x","article-title":"Identification of PCR\u2010based markers linked to the powdery\u2010mildew\u2010resistance gene Pl1 from Malus robusta in cultivated apple","volume":"114","author":"T Markussen","year":"1995","journal-title":"Plant Breeding"},{"issue":"1","key":"pone.0357988.ref025","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1111\/j.1471-8286.2005.01155.x","article-title":"genalex 6: genetic analysis in Excel. 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