{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T18:04:42Z","timestamp":1789668282677,"version":"4.0.1"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Osteoarthritis (OA), a prevalent degenerative joint disorder, is driven by chondrocyte anabolic-catabolic imbalance, aberrant inflammation, and mitochondrial dysfunction. Disease-modifying therapies targeting these pathological processes remain scarce, limiting effective clinical intervention. Herein, we demonstrate an isoquercitrin (IQ)-based chondroprotective strategy that rectifies osteoarthritis-related chondrocyte impairment by targeting\n                    <jats:italic>AKT1<\/jats:italic>\n                    . IQ first reverses IL-1\u03b2-induced chondrocyte proliferation arrest, shifts metabolic homeostasis toward anabolism, and mitigates pro-inflammatory responses. It simultaneously restores mitochondrial homeostasis by normalizing dynamic balance. Mechanistically, IQ binds to AKT1 with high affinity, and\n                    <jats:italic>AKT1<\/jats:italic>\n                    overexpression abolishes its protective effects\u2014confirming\n                    <jats:italic>AKT1<\/jats:italic>\n                    as a key mediator. This approach re-establishes chondrocyte functional homeostasis by coupling anti-inflammatory, metabolic-regulatory, and mitochondrial-stabilizing activities. This work highlights IQ as a promising candidate for osteoarthritis intervention and provides a mechanistic framework linking\n                    <jats:italic>AKT1<\/jats:italic>\n                    targeting to chondroprotection.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0357989","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T17:25:06Z","timestamp":1789665906000},"page":"e0357989","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isoquercitrin ameliorates chondrocyte dysfunction in osteoarthritis by targeting AKT1-mediated mitochondrial dynamics regulation"],"prefix":"10.1371","volume":"21","author":[{"given":"Yuan","family":"Ding","sequence":"first","affiliation":[{"name":"Yantaishan Hospital","place":["Yantai, Shandong Province, China"],"department":["Department of Rheumatology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Yantaishan Hospital","place":["Yantai, Shandong Province, China"],"department":["Department of Nephrology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihua","family":"Zong","sequence":"additional","affiliation":[{"name":"Yantaishan Hospital","place":["Yantai, Shandong Province, China"],"department":["Department of Rheumatology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wen","sequence":"additional","affiliation":[{"name":"Yantaishan Hospital","place":["Yantai, Shandong Province, China"],"department":["Department of Rheumatology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3472-5271","authenticated-orcid":true,"given":"Luyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Yantaishan Hospital","place":["Yantai, Shandong Province, China"],"department":["Department of Rheumatology and Immunology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"issue":"4","key":"pone.0357989.ref001","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1080\/00325481.2020.1730669","article-title":"Osteoarthritis and inflammation: a serious disease with overlapping phenotypic patterns","volume":"132","author":"F Berenbaum","year":"2020","journal-title":"Postgrad Med"},{"issue":"1","key":"pone.0357989.ref002","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s13075-021-02420-2","article-title":"Circular RNAs in osteoarthritis: indispensable regulators 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