{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:58:03Z","timestamp":1791482283240,"version":"4.3.4"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The accumulation of plastics and heavy metals (HMs) in the environment has become a significant global concern due to their persistence, ubiquity, and potential ecological impacts. Atmospheric micro- and nanoplastics (MP, NP) are capable of undergoing long-range transport and can be deposited onto plant shoots through precipitation, thereby exposing aerial plant tissues to these particulate contaminants. In this study, the effects of varying concentrations of polystyrene nanoplastics (PSNPs) (0, 10, 50, 100, 200, and 400 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    ), applied via foliar spraying, and cadmium (Cd) (0 and 5 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    ) were evaluated on the growth parameters and chemical composition of spinach (\n                    <jats:italic>Spinacia oleracea L.<\/jats:italic>\n                    ) grown under hydroponic conditions. The experiment was carried out over three exposure durations\u20142, 4, and 6 weeks\u2014designated as t\n                    <jats:sub>1<\/jats:sub>\n                    , t\n                    <jats:sub>2<\/jats:sub>\n                    , and t\n                    <jats:sub>3<\/jats:sub>\n                    , respectively. Fifteen-day-old seedlings were transplanted into hydroponic containers and sampled at the end of each exposure period. The results demonstrated that PSNPs had a significant impact on plant growth and biomass accumulation. At t\n                    <jats:sub>3<\/jats:sub>\n                    , exposure to 200 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    of PSNPs resulted in an 83.33% and 87.34% increase in the dry weight of shoots and roots, respectively, compared to the control. However, this positive trend reversed at higher concentrations; at 400 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    , shoot and root dry weights decreased by 49.87% and 27.87%, respectively, relative to the 200 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    treatment. Cd concentration in the shoot at t\n                    <jats:sub>3<\/jats:sub>\n                    increased by 72.28% at 50 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    PSNPs compared to the control but declined by 31.32% at 400 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    . The Cd translocation factor (TF) from root to shoot rose sharply with increasing PSNPs concentrations, reaching a peak increase of 194% at 400 mg L\n                    <jats:sup>-1<\/jats:sup>\n                    at t\n                    <jats:sub>3<\/jats:sub>\n                    . Additionally, TF values exhibited a positive correlation with plant age, suggesting enhanced TF in older plants. These results highlight the potential risk of PSNPs in Cd-contaminated environments, where they may modify Cd uptake, distribution and root-to-shoot translocation in spinach plants. By facilitating Cd transfer to shoots, particularly under prolonged exposure, PSNPs could increase the likelihood of Cd accumulation in edible tissues. This interaction raises concerns regarding the safety of leafy vegetables grown in agricultural systems co-contaminated with MPs\/NPs and HMs.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0358424","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T17:41:33Z","timestamp":1791481293000},"page":"e0358424","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Foliar polystyrene nanoplastics modulate cadmium uptake and root-to-shoot translocation in hydroponic Spinacia oleracea L.: Time- and concentration-dependent responses"],"prefix":"10.1371","volume":"21","author":[{"given":"Mohamad Kazem","family":"Dehghani","sequence":"first","affiliation":[{"name":"School of Agriculture, Shiraz University","place":["Shiraz, Iran"],"department":["Department of Soil Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4410-4963","authenticated-orcid":true,"given":"Reza","family":"Ghasemi-Fasaei","sequence":"additional","affiliation":[{"name":"School of Agriculture, Shiraz University","place":["Shiraz, Iran"],"department":["Department of Soil Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajjad","family":"Abbasi","sequence":"additional","affiliation":[{"name":"College of Science, Shiraz University","place":["Shiraz, Iran"],"department":["Department of Earth Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Mojiri","sequence":"additional","affiliation":[{"name":"School of Sustainable Engineering and the Built Environment, Arizona State University","place":["Tempe, Arizona, United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"pone.0358424.ref001","author":"E Plastics","year":"2018"},{"key":"pone.0358424.ref002","doi-asserted-by":"crossref","first-page":"114402","DOI":"10.1016\/j.envres.2022.114402","article-title":"Effect of foliar and root exposure to polymethyl methacrylate microplastics on biochemistry, ultrastructure, and arsenic accumulation in Brassica campestris L","volume":"215","author":"R Dong","year":"2022","journal-title":"Environ Res"},{"issue":"7","key":"pone.0358424.ref003","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.1700782","article-title":"Production, use, and fate of all plastics ever made","volume":"3","author":"R Geyer","year":"2017","journal-title":"Sci Adv"},{"key":"pone.0358424.ref004","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.marenvres.2016.05.012","article-title":"Plastics and microplastics in the oceans: From emerging pollutants to emerged threat","volume":"128","author":"CG Avio","year":"2017","journal-title":"Mar Environ Res"},{"key":"pone.0358424.ref005","doi-asserted-by":"crossref","first-page":"114889","DOI":"10.1016\/j.envpol.2020.114889","article-title":"Textile microfibers reaching aquatic environments: A new estimation approach","volume":"265","author":"F Belzagui","year":"2020","journal-title":"Environ Pollut"},{"key":"pone.0358424.ref006","doi-asserted-by":"crossref","first-page":"111478","DOI":"10.1016\/j.marpolbul.2020.111478","article-title":"Sources, spatial distribution and characteristics of marine litter along the west coast of Qatar","volume":"159","author":"S Veerasingam","year":"2020","journal-title":"Mar Pollut Bull"},{"key":"pone.0358424.ref007","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.marpolbul.2018.11.022","article-title":"Microplastics: Finding a consensus on the definition","volume":"138","author":"JP Frias","year":"2019","journal-title":"Mar Pollut Bull"},{"issue":"3","key":"pone.0358424.ref008","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1021\/acs.est.8b05297","article-title":"Are We Speaking the Same Language? 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