{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T18:15:06Z","timestamp":1790014506507,"version":"4.0.1"},"reference-count":106,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2024-03847"],"award-info":[{"award-number":["RGPIN-2024-03847"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Most animals face periods of food scarcity, and pathogens are ever-present. Therefore, selection will favour animals that can maintain immune defense during starvation, despite declining resources. Using the caterpillar\n                    <jats:italic>Manduca sexta<\/jats:italic>\n                    as a model system, we show how two days of starvation reconfigures the immune system. Metabolomic and proteomic analysis of the plasma showed that starvation shifted the immune system towards a more \u2018pro-inflammatory\u2019 state, with increased activity in the phenoloxidase\/eumelanin pathways relative to controls. Melanization occurred more rapidly in starved caterpillars, demonstrating that these molecular changes had functional significance. This chronic inflammation correlated with a decline in the abundance of the important antioxidant glutathione (GSH) in the plasma. Increasing GSH in the plasma either by injection or diet reduced melanization in starved caterpillars, suggesting that the decline in GSH abundance could contribute to the increase in inflammation. Although starvation enhanced phenoloxidase pathway activity, it also reduced nodulation, suggesting a decline in cell-mediated immunity. Nevertheless, we found that starved caterpillars had fewer bacteria in their hemolymph (i.e., colony forming units, CFUs) 3 h after infection with live\n                    <jats:italic>Bacillus cereus<\/jats:italic>\n                    than did fed controls or weight-matched controls. However, increasing GSH in starved caterpillars increased mortality from\n                    <jats:italic>B. cereus<\/jats:italic>\n                    infection. We suggest that the upregulation of the phenoloxidase\/eumelanin pathways during starvation is beneficial because it partially compensates for concurrent declines in other immune components. Without this chronic inflammation, our results suggest that caterpillars would experience an even greater decline in disease resistance during starvation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0358460","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:42:42Z","timestamp":1790012562000},"page":"e0358460","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["An upside to chronic inflammation? Starvation reconfigures immune pathways in Manduca sexta, producing chronic inflammation that may help defend against bacterial infection"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5973-571X","authenticated-orcid":true,"given":"Shelley A.","family":"Adamo","sequence":"first","affiliation":[{"name":"Dalhousie University","place":["Halifax, Nova Scotia, Canada"],"department":["Department of Psychology and Neuroscience"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine R.","family":"Burns","sequence":"additional","affiliation":[{"name":"Dalhousie University","place":["Halifax, Nova Scotia, Canada"],"department":["Department of Psychology and Neuroscience"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0281-4142","authenticated-orcid":true,"given":"Megan H.","family":"Englehardt","sequence":"additional","affiliation":[{"name":"Dalhousie University","place":["Halifax, Nova Scotia, Canada"],"department":["Department of Psychology and Neuroscience"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arisha","family":"Grabtchak","sequence":"additional","affiliation":[{"name":"Dalhousie University","place":["Halifax, Nova Scotia, Canada"],"department":["Department of Psychology and Neuroscience"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7341-7003","authenticated-orcid":true,"given":"Rose","family":"Kearney","sequence":"additional","affiliation":[{"name":"Dalhousie University","place":["Halifax, Nova Scotia, Canada"],"department":["Department of Psychology and Neuroscience"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire A. 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