{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:56:44Z","timestamp":1791395804844,"version":"4.3.3"},"reference-count":41,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    is a flagellated commensal protozoan that can infect both humans and animals. Infection can result in clinical signs such as diarrhea or, more often, the infection can be subclinical. Within the\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    species, there are several major genetic assemblages, A \u2013 H. Certain assemblages are species specific (C and D in dogs, F in cats) while assemblages A and B can infect humans as well as other mammals. This retrospective study used data collected from a commercial reference laboratory (Antech Diagnostics, Mars Petcare Science &amp; Diagnostics). Real-time PCR data using fecal qPCR panel (KeyScreen\u2122 GI Parasite PCR) for\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    and potential zoonotic assemblages A\/B in 157,616 domestic cat stool samples from North America collected between March 8, 2022, to August 31, 2023, was used. Variables including age, sex, altered status, season, and geographic region were assessed to identify associations with cats that were detected for\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    and potential zoonotic assemblages A\/B. The overall prevalence of\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    was 6.3% (9,962\/157,616) and of those detected, 18.5% (1,840\/9,962) were assemblages A\/B. Cats that were\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    detected were more likely to be male, intact, less than one-year old, and in the Midwest. In general, lowest detection occurred during the summer months. Zoonotic potential detection was highest in cats aged less than one-year (19.7%; 1,011\/5,137). These data highlight the importance of routine fecal screening in domestic cats to detect parasites, particularly those, like\n                    <jats:italic>Giardia<\/jats:italic>\n                    , of One Health concern, and provides prevalence, and risk factor information that aids clinical veterinary decision-making.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0358546","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:24:10Z","timestamp":1791393850000},"page":"e0358546","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Giardia duodenalis in domestic cats from the US and Canada: Prevalence and zoonotic potential"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8787-9469","authenticated-orcid":true,"given":"Margaret","family":"Wingerd","sequence":"first","affiliation":[{"name":"VCA SouthPaws Veterinary Specialists & Emergency Center","place":["Fairfax, Virginia, United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9137-0335","authenticated-orcid":true,"given":"Anne","family":"Kimmerlein","sequence":"additional","affiliation":[{"name":"Mars Veterinary Health","place":["Los Angeles, California, United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5596-7380","authenticated-orcid":true,"given":"Pablo D.","family":"Jimenez Castro","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]},{"name":"Grupo de Parasitologia Veterinaria, Universidad Nacional de Colombia","place":["Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle D.","family":"Evason","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Willcox","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia E.","family":"Lozoya","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Tereski","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J.","family":"Bergman","sequence":"additional","affiliation":[{"name":"Mars Veterinary Health","place":["Los Angeles, California, United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian M.","family":"Leutenegger","sequence":"additional","affiliation":[{"name":"Mars Petcare Science & Diagnostics","place":["Loveland, Colorado, United States of America"],"department":["Antech Diagnostics"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie E.","family":"Kerl","sequence":"additional","affiliation":[{"name":"Mars Veterinary Health","place":["Los Angeles, California, United States 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