{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:50:59Z","timestamp":1792086659473,"version":"4.4.0"},"reference-count":13,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"\n Objective<\/jats:title>\n To evaluate the clinical impact of the revised antral follicle count (AFC) threshold on polycystic ovary syndrome (PCOS) diagnosis.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A retrospective cross-sectional observational study including women diagnosed using the Rotterdam criteria, stratified by AFC: 12\u201319 (low AFC) and \u226520 (high AFC). Clinical, metabolic, and hormonal parameters were compared against controls and amongst low and high AFC group.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Of 336 PCOS women, 208 (62%) had an AFC between 12 and 19 and would not meet the revised \u226520 follicle threshold for the diagnosis of polycystic morphology. These women had worse metabolic and androgenic profiles than controls, and were similar to the high AFC group.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Excluding low AFC women may misclassify patients who require clinical care.<\/jats:p>\n <\/jats:sec>","DOI":"10.1371\/journal.pone.0358554","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:33:51Z","timestamp":1792085631000},"page":"e0358554","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of revised antral follicle count threshold on PCOS diagnosis: A comparative clinical study"],"prefix":"10.1371","volume":"21","author":[{"given":"Saloni","family":"Kamboj","sequence":"first","affiliation":[{"name":"All India Institute of Medical Sciences","place":["New Delhi, Delhi, India"],"department":["Department of Obstetrics and Gynaecology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7837-5361","authenticated-orcid":true,"given":"Neena","family":"Malhotra","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences","place":["New Delhi, Delhi, India"],"department":["Department of Obstetrics and Gynaecology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reeta","family":"Mahey","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences","place":["New Delhi, Delhi, India"],"department":["Department of Obstetrics and Gynaecology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taruna","family":"Arora","sequence":"additional","affiliation":[{"name":"Indian Council of Medical Research","place":["New Delhi, Delhi, India"],"department":["Division of Reproductive Biology, Maternal and Child Health"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Goyal","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences","place":["New Delhi, Delhi, India"],"department":["Department of Obstetrics and Gynaecology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Snigdha","family":"Soni","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences","place":["New Delhi, Delhi, India"],"department":["Department of Obstetrics and Gynaecology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshu","family":"Jyoti","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences","place":["New Delhi, Delhi, India"],"department":["Department of Obstetrics and Gynaecology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spondita","family":"Banerjee","sequence":"additional","affiliation":[{"name":"All India Institute of Medical 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syndrome","volume":"81","author":"Rotterdam ESHRE\/ASRM-Sponsored PCOS Consensus Workshop Group","year":"2004","journal-title":"Fertil Steril"},{"issue":"3","key":"pone.0358554.ref003","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.fertnstert.2018.05.004","article-title":"Recommendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome","volume":"110","author":"HJ Teede","year":"2018","journal-title":"Fertil Steril"},{"issue":"10","key":"pone.0358554.ref004","doi-asserted-by":"crossref","DOI":"10.1001\/jamanetworkopen.2024.40583","article-title":"Prevalence, phenotypes, and comorbidities of polycystic ovary syndrome among Indian women","volume":"7","author":"MA Ganie","year":"2024","journal-title":"JAMA Netw Open"},{"key":"pone.0358554.ref005","first-page":"114","article-title":"Prevalence and risk factors for metabolic syndrome in individuals with polycystic ovary syndrome: An institutional study","volume":"18","author":"R Anishetty","year":"2024","journal-title":"Res J Med Sci"},{"issue":"5","key":"pone.0358554.ref006","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1016\/j.fertnstert.2014.01.049","article-title":"Complete phenotypic and metabolic profiles of a large consecutive cohort of untreated Korean women with polycystic ovary syndrome","volume":"101","author":"JJ Kim","year":"2014","journal-title":"Fertil Steril"},{"issue":"3","key":"pone.0358554.ref007","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1093\/humrep\/deg115","article-title":"Ultrasound examination of polycystic ovaries: Is it worth counting the follicles?","volume":"18","author":"S Jonard","year":"2003","journal-title":"Hum Reprod"},{"issue":"5","key":"pone.0358554.ref008","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1002\/uog.5393","article-title":"Ovarian volume and follicle number in the diagnosis of polycystic ovary syndrome in Chinese women","volume":"32","author":"Y Chen","year":"2008","journal-title":"Ultrasound Obstet Gynecol"},{"issue":"3","key":"pone.0358554.ref009","first-page":"146","article-title":"2D and 3D trans-vaginal sonography to determine cut-offs for ovarian volume and follicle number per ovary for diagnosis of polycystic ovary syndrome in Indian women","volume":"19","author":"S Kar","year":"2018","journal-title":"J Reprod Infertil"},{"key":"pone.0358554.ref010","doi-asserted-by":"crossref","first-page":"26334941241270372","DOI":"10.1177\/26334941241270372","article-title":"Validation of the follicular and ovarian thresholds by an 18-MHz ultrasound imaging in polycystic ovary syndrome: A pilot cutoff for North African patients","volume":"18","author":"T Ach","year":"2024","journal-title":"Ther Adv Reprod Health"},{"issue":"6","key":"pone.0358554.ref011","doi-asserted-by":"crossref","first-page":"545","DOI":"10.3109\/09513590.2013.777415","article-title":"The role of serum anti-M\u00fcllerian hormone (AMH) in the hormonal diagnosis of polycystic ovary syndrome","volume":"29","author":"L Casadei","year":"2013","journal-title":"Gynecol Endocrinol"},{"issue":"11","key":"pone.0358554.ref012","doi-asserted-by":"crossref","first-page":"3123","DOI":"10.1093\/humrep\/der297","article-title":"Diagnosis of polycystic ovary syndrome (PCOS): Revisiting the threshold values of follicle count on ultrasound and of the serum AMH level for the definition of polycystic ovaries","volume":"26","author":"D Dewailly","year":"2011","journal-title":"Hum Reprod"},{"key":"pone.0358554.ref013","article-title":"Cardiometabolic markers and serum amh levels in PCOS: Can AMH serve as a","author":"MA Ganie","year":"2023","journal-title":"Journal of Clinical Endocrinology & Metabolism"}],"container-title":["PLOS 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