{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:01:34Z","timestamp":1790618494118,"version":"4.1.0"},"reference-count":60,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016929","name":"PETRONAS","doi-asserted-by":"publisher","award":["YUTP-FRG 015LC0-557"],"award-info":[{"award-number":["YUTP-FRG 015LC0-557"]}],"id":[{"id":"10.13039\/100016929","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016929","name":"PETRONAS","doi-asserted-by":"publisher","award":["YUTP-PRG 015PBC-042"],"award-info":[{"award-number":["YUTP-PRG 015PBC-042"]}],"id":[{"id":"10.13039\/100016929","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009614","name":"Petroleum Technology Development Fund","doi-asserted-by":"publisher","award":["POSS2023000000095751"],"award-info":[{"award-number":["POSS2023000000095751"]}],"id":[{"id":"10.13039\/501100009614","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Wax precipitation in crude oil pipelines is a challenge to production. Current inhibitors raise concerns about toxicity and environmental impact. Hence, the need for sustainable alternatives arises. This study aims to explore the\n                    <jats:italic>Vitex doniana<\/jats:italic>\n                    -assisted synthesis, characterization, and application of zinc oxide nanoparticles as crude oil wax inhibitors. The nanomaterial was characterized using Fourier Transform Infrared Spectroscopy, Gas Chromatography\u2013Mass Spectrometry, X-Ray Diffraction, Field Emission Scanning Electron Microscopy, Energy-Dispersive X-Ray Spectroscopy, and Thermogravimetric Analysis. The application of 0.04 wt% zinc oxide was evaluated based on wax appearance temperature, rheological properties, and wax-inhibition efficiency. Fourier Transform Infrared Spectroscopy analysis on the ethanolic\n                    <jats:italic>Vitex doniana<\/jats:italic>\n                    leaf extract confirmed the presence of alcohols, phenolics, carbonyls, aromatics, proteins, and polyphenols responsible for nanoparticle reduction and stabilization of the nanoparticle. Gas Chromatography\u2013Mass Spectrometry of the extracts identified 18 distinct peaks in the organic profile, revealing a mixture of esters, aldehydes, acids, and heterocycles. The dominance of n\u2011hexane (34.9%) and glycidyl oleate (21.2%) highlights hydrocarbon and ester fractions as major components. X-Ray Diffraction analysis confirmed zinc oxide nanocrystals in a pure hexagonal wurtzite phase (a\u2009=\u20093.243 \u00c5, c\u2009=\u20095.195 \u00c5) with an average crystallite size of 36.1 nm and microstrain of 1.4\u2009\u00d7\u200910\u2009\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2009\u2074, evidencing high crystallinity and suitability for optoelectronic and photocatalytic applications. Field Emission Scanning Electron Microscopy-Energy-Dispersive X-Ray Spectroscopy confirmed elemental composition of 64.3 wt% Zn, 20.6 wt% O, 15.1 wt% residual C, and trace N, evidencing high purity. Thermogravimetric Analysis indicated high thermal stability with minimal mass loss (1.308% at ~118.7 \u00b0C, 3.340% at ~227.9 \u00b0C, and 7.427% at ~391.8 \u00b0C; total mass loss\u2009&lt;\u200912.1% up to ~800 \u00b0C). Rheological analysis showed a reduction in viscosity of up to 56.2% at 23 \u00b0C when crude oil was blended with zinc oxide nanoparticles. The Cross-Polarized Microscopy test showed that zinc oxide modified the wax structure, resulting in clustered morphology, suggesting a cocrystallization mechanism. The Arrhenius plot of viscosity showed that zinc oxide reduced the flow activation energy from 213.6 kJ mol\u2009\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2009\u00b9 to 207.6 kJ mol\u2009\n                    <jats:sup>\u2212<\/jats:sup>\n                    \u2009\u00b9, confirming the effectiveness of zinc oxide nanoparticles in improving crude oil flow by disrupting wax-crystal networks. These findings confirm the successful production of high-quality, thermally stable, and surface-functionalized zinc oxide nanoparticles, suitable for various industrial applications, especially waxy crude flow assurance.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0358989","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:39:06Z","timestamp":1790617146000},"page":"e0358989","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and characterization of sustainable Zinc Oxide nanoparticles for waxy crude flow assurance application"],"prefix":"10.1371","volume":"21","author":[{"given":"Abubakar","family":"Aji","sequence":"first","affiliation":[{"name":"Universiti Teknologi PETRONAS (UTP)","place":["Seri Iskandar, Perak Darul Ridzuan, Malaysia"],"department":["Department of Petroleum Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisham Khaled","family":"Ben Mahmud","sequence":"additional","affiliation":[{"name":"Sohar University","place":["Oman"],"department":["Faculty of Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Abduljabbar","sequence":"additional","affiliation":[{"name":"Universiti Teknologi PETRONAS (UTP)","place":["Seri Iskandar, Perak Darul Ridzuan, Malaysia"],"department":["Department of Petroleum Engineering"]},{"name":"Institute of Sustainable Energy & Resources, Universiti Teknologi PETRONAS (UTP)","place":["Seri Iskandar, Perak Darul Ridzuan, Malaysia"],"department":["Center of Flow Assurance"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibnelwaleed A.","family":"Hussein","sequence":"additional","affiliation":[{"name":"College of Engineering, Qatar University","place":["Doha, Qatar"],"department":["Gas Processing Center"]},{"name":"College of Engineering, Qatar University","place":["Doha, Qatar"],"department":["Chemical Engineering Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nik Ahmad Nizam Nik","family":"Malek","sequence":"additional","affiliation":[{"name":"Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina Institute for Scientific and Industrial Research (ISI-ISIR), Universiti Teknologi Malaysia (UTM)","place":["Skudai, Johor, Malaysia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor Damilare","family":"Ola","sequence":"additional","affiliation":[{"name":"Universiti Teknologi PETRONAS (UTP)","place":["Seri Iskandar, Perak Darul Ridzuan, Malaysia"],"department":["Department of Petroleum Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5200-8491","authenticated-orcid":true,"given":"Mysara Eissa","family":"Mohyaldinn","sequence":"additional","affiliation":[{"name":"Universiti Teknologi PETRONAS (UTP)","place":["Seri Iskandar, Perak Darul Ridzuan, Malaysia"],"department":["Department of Petroleum Engineering"]},{"name":"Institute of Sustainable Energy & Resources, Universiti Teknologi PETRONAS (UTP)","place":["Seri Iskandar, Perak Darul Ridzuan, Malaysia"],"department":["Center of Flow Assurance"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"issue":"1","key":"pone.0358989.ref001","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0313394","article-title":"Improving paraffin precipitate inhibition using glycine and Palm-based Methyl Ester Sulfonate (MES) eco-friendly inhibitors","volume":"20","author":"NB Surpina","year":"2025","journal-title":"PLoS One"},{"issue":"6","key":"pone.0358989.ref002","doi-asserted-by":"crossref","first-page":"5381","DOI":"10.1021\/acs.energyfuels.3c05154","article-title":"Development of Poly(acrylate-co-benzyl maleate) Copolymers as Wax Deposition 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