{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:02:33Z","timestamp":1790359353102,"version":"4.1.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100022866","name":"Mutah University","doi-asserted-by":"publisher","award":["879\/2024"],"award-info":[{"award-number":["879\/2024"]}],"id":[{"id":"10.13039\/100022866","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The aim of the current research project is to evaluate the potential cytotoxicity of the aqueous extract of\n                    <jats:italic>Artemisia -albaherba<\/jats:italic>\n                    aerial parts against selected cancer cell lines. The widely grown plant material was collected and extracted. The crude aqueous extract was subjected to MTT analysis to assess potential cytotoxicity and cell viability. The cell lines utilized include: Normal Human Umbilical Vein Endothelial cell line EA (HUVEC; cat. no. CRL-1730; ATCC), Triple Negative Breast cancer cell line, MDA MB 231 (HTB-26), Human Pancreatic Ductal Adenocarcinoma cell line PANC1 (CRL-1469), Human Colorectal Carcinoma HCT 116 (CCL-247) cell lines. Cisplatin and doxorubicin serve as reference agents, and the media vehicle serves as a negative control. The extract was analysed by UHPLC\/MS-MS. Three of the detected bioactive phytoconstituents, namely liquiritigenin, isofraxidin, and artemisinin, were docked against three cancer-associated protein targets corresponding to the three investigated cancer cell lines. The selected targets were TP53\/p53 in the HCT-116 colorectal cancer cell line, AKT1 in the MDA cell line, and KRAS in the PANC-1 pancreatic cancer cell line. A marked decrease in cell viability was observed in the human colon and breast cancer cell lines at plant extract concentrations of 0.046\u201350 mg\/mL. The highest cytotoxicity, around 45%, was observed against human colon cancer cells at concentrations of 12.5\u201350 mg\/mL. And the cytotoxicity was around 40% against breast cancer cells at a concentration of 50 mg\/mL. The plant remedy exhibited moderate cytotoxicity against pancreatic cancer cells, with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of approximately 20% at the highest tested concentration. The biocompatibility of the aqueous extract was predicted; hence, normal epithelial cells retained their viability at 1.5 mg\/mL and showed a moderate-to-weak effect at higher concentrations. The herbal extract exhibits the highest IC\n                    <jats:sub>50<\/jats:sub>\n                    values against EA and PANC1 of 220\u2013240 \u00b5g\/mL, whereas against MDA-MB-231 and HCT-116 cells, the IC\n                    <jats:sub>50<\/jats:sub>\n                    values are significantly lower at 80\u2013100 \u00b5g\/mL. This indicates greater sensitivity of breast and colon cancer cell lines to the natural product. The simulation analyses demonstrate that isofraxidin and liquiritigenin have better binding affinity to the selected proteins than artemisinin. The best overall complexes were TP53-isofraxidin and TP53-liquiritigenin, with docking scores around \u22128 kcal\/mol, followed by KRAS-liquiritigenin and AKT1-isofraxidin. To sum up,\n                    <jats:italic>A. herb alba<\/jats:italic>\n                    has a potential selective anticancer effect that may be attributed to the phenolic compounds constituents. These promising findings warrant further study to elucidate its mechanism of action, safety profile, and to predict the optimal dosage formulation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359068","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:43:37Z","timestamp":1790358217000},"page":"e0359068","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the cytotoxic potential of Artemisia herba-alba crude extract and key phytoconstituents using in vitro assays and molecular docking insights"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2635-3619","authenticated-orcid":true,"given":"Lidia Kamal","family":"Al-Halaseh","sequence":"first","affiliation":[{"name":"Mutah University","place":["Al-Karak, Jordan"],"department":["Department of Pharmaceutical Chemistry, Faculty of 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M.","family":"Al-Zregat","sequence":"additional","affiliation":[{"name":"Mutah University","place":["Al-Karak, Jordan"],"department":["Department of Medicine, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaid S.","family":"Amareen","sequence":"additional","affiliation":[{"name":"Mutah University","place":["Al-Karak, Jordan"],"department":["Department of Medicine, Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manal Nahar","family":"Al Soub","sequence":"additional","affiliation":[{"name":"Mutah University","place":["Al-Karak, Jordan"],"department":["Department of Pharmaceutical Chemistry, Faculty of Pharmacy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine N.","family":"Abdelhadi","sequence":"additional","affiliation":[{"name":"Aqaba University of Technology","place":["Aqaba, Jordan"],"department":["Department of clinical pharmacy and pharmacy practice, Faculty of 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