{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:54:47Z","timestamp":1792173287576,"version":"4.4.0"},"reference-count":53,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["88887.711815\/2022-00"],"award-info":[{"award-number":["88887.711815\/2022-00"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["E-26\/200.444\/2023"],"award-info":[{"award-number":["E-26\/200.444\/2023"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["441653\/2024-3"],"award-info":[{"award-number":["441653\/2024-3"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302503 2021-8"],"award-info":[{"award-number":["302503 2021-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Astrovirus, cardiovirus and rotavirus are RNA viruses widely distributed among humans, domestic and wild mammals, including felids, occasionally associated with fatal outbreaks and highly resistant to environmental conditions. Despite this, there are no reports of these viruses in New World felids. The aim of this study was to investigate the presence of astrovirus, rotavirus and cardiovirus in non-domestic felids under human care or in the wild, across multiple Brazilian biomes (i.e., Amazon Forest, Atlantic Forest, Caatinga, and Cerrado). Biological samples (e.g., blood, oral and rectal swabs, and frozen tissues) were submitted to reverse transcriptase polymerase chain reaction (RT-PCR) for astrovirus detection and real-time RT-PCR for rotavirus and cardiovirus, followed by sequencing. Fifty wild felids were analyzed, comprising 40 free-ranging and 10 captive animals: 19 ocelots (\n                    <jats:italic>Leopardus pardalis<\/jats:italic>\n                    ), 12 pumas (\n                    <jats:italic>Puma concolor<\/jats:italic>\n                    ), seven jaguarundis (\n                    <jats:italic>Herpailurus yagouaroundi<\/jats:italic>\n                    ), six southern tiger cats (\n                    <jats:italic>Leopardus guttulus<\/jats:italic>\n                    ), three margays (\n                    <jats:italic>Leopardus wiedii<\/jats:italic>\n                    ) and three jaguars (\n                    <jats:italic>Panthera onca<\/jats:italic>\n                    ). An astrovirus was detected in rectal swab of a healthy captive southern tiger cat, while a genotype G3P[3]\n                    <jats:italic>Rotavirus alphagastroenteritidis<\/jats:italic>\n                    (Rotavirus A) was detected in intestine of a roadkill puma. All felid samples were negative for cardiovirus. Morphological analyses of available formalin-fixed tissues of the rotavirus-positive puma were inconclusive due to autolysis. To the authors\u2019 knowledge, this is the first molecular detection of astrovirus and rotavirus in Neotropical wild felid species.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359169","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:26:01Z","timestamp":1792171561000},"page":"e0359169","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular screening of selected RNA viruses in neotropical wild felids of Brazil"],"prefix":"10.1371","volume":"21","author":[{"given":"Michelle","family":"Colpani","sequence":"first","affiliation":[{"name":"Universidade Estadual Paulista, J\u00falio de Mesquita Filho","place":["Botucatu, S\u00e3o Paulo, Brazil"],"department":["Faculdade de Medicina Veterin\u00e1ria e 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Enrique","family":"Navas-Suarez","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"],"department":["Faculdade de Medicina Veterin\u00e1ria e Zootecnia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Alejandra","family":"Arias-Lugo","sequence":"additional","affiliation":[{"name":"Universidade de S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"],"department":["Faculdade de Medicina Veterin\u00e1ria e Zootecnia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo Gon\u00e7alves","family":"Morato","sequence":"additional","affiliation":[{"name":"Panthera","place":["New York, New York, United States of America"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rose Lilian","family":"Gasparini-Morato","sequence":"additional","affiliation":[{"name":"Centro Nacional de Pesquisa e Conserva\u00e7\u00e3o de Mam\u00edferos Carn\u00edvoros","place":["Atibaia, S\u00e3o 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