{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:01:09Z","timestamp":1790359269097,"version":"4.1.0"},"reference-count":99,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018226","name":"University of the South Pacific","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    The Ba River in Fiji is a critical livelihood source for bivalve harvesters, yet increasing anthropogenic activities threaten the safety of its aquatic food sources. This study comprehensively examines the microbial communities associated with freshwater bivalves (\n                    <jats:italic>Batissa violacea<\/jats:italic>\n                    ) and river sediments, focusing on antibiotic resistance and heavy metal (HM) tolerance. Samples from soft tissues, gills, and sediments were subjected to Most Probable Number (MPN) tests, antibiotic susceptibility assays, HM tolerance analysis, and 16S rRNA gene sequencing to identify microbial taxa and their functional characteristics. Several pathogenic bacteria were isolated and identified, including\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    ,\n                    <jats:italic>Vibrio parahaemolyticus<\/jats:italic>\n                    ,\n                    <jats:italic>Salmonella<\/jats:italic>\n                    spp., and thermotolerant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    . Most isolates exhibited multidrug resistance, particularly against rifampicin, vancomycin, penicillin, streptomycin, and tetracycline, posing potential risks to public health and food safety. Conversely, HM tolerance analysis revealed limited microbial resistance to zinc, copper, cadmium, chromium, and lead, predominantly at concentrations of 1 mM. The presence of these antibiotic-resistant and HM-tolerant microbes underscores the critical interplay between environmental contamination and microbial evolution, presenting challenges for both ecosystem health and human consumption safety. The findings highlight the urgent need for integrated environmental management and interventions to mitigate microbial contamination in aquatic systems.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359203","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T17:34:17Z","timestamp":1790357657000},"page":"e0359203","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibiotic-resistant pathogens and heavy metal-tolerant bacteria in Batissa violacea: A study from the Ba River, Fiji"],"prefix":"10.1371","volume":"21","author":[{"given":"Divya Darshika","family":"Mala","sequence":"first","affiliation":[{"name":"Discipline of Biological and Chemical Sciences, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, The University of the South Pacific","place":["Suva, Fiji"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3529-1245","authenticated-orcid":true,"given":"Ashneel Ajay","family":"Singh","sequence":"additional","affiliation":[{"name":"Discipline of Biological and Chemical Sciences, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, The University of the South Pacific","place":["Suva, Fiji"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh","family":"Subramani","sequence":"additional","affiliation":[{"name":"Discipline of Biological and Chemical Sciences, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, The University of the South Pacific","place":["Suva, Fiji"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6019-7421","authenticated-orcid":true,"given":"Sathiyaraj","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"College of Natural Science, Seoul Women\u2019s University","place":["Seoul, Korea"],"department":["Department of Biology and Environmental Technology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taitusi","family":"Taufa","sequence":"additional","affiliation":[{"name":"Discipline of Biological and Chemical Sciences, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, The University of the South Pacific","place":["Suva, Fiji"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0255-312X","authenticated-orcid":true,"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[{"name":"Discipline of Biological and Chemical Sciences, School of Agriculture, Geography, Environment, Ocean and Natural Sciences, The University of the South Pacific","place":["Suva, Fiji"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Mani","sequence":"additional","affiliation":[{"name":"Discipline of Biological 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Gyawali","year":"2020","journal-title":"Current Opinion in Environmental Science & Health"},{"issue":"9","key":"pone.0359203.ref010","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02156-17","article-title":"Environmental controls of oyster-pathogenic Vibrio spp. in Oregon estuaries and a shellfish hatchery","volume":"84","author":"MR Gradoville","year":"2018","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"pone.0359203.ref011","doi-asserted-by":"crossref","first-page":"216","DOI":"10.47836\/ifrj.32.1.16","article-title":"Bacterial and parasitic contamination in blood cockles (Anadara antiquata Linnaeus, 1758), and effect of depuration on bacterial load using simple bio-filter in closed circulatory system","volume":"32","author":"AA Singh","year":"2025","journal-title":"IFRJ"},{"issue":"19","key":"pone.0359203.ref012","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.3390\/w13192660","article-title":"Various natural and anthropogenic factors responsible for 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River","volume":"511","author":"C Staley","year":"2015","journal-title":"Sci Total Environ"},{"key":"pone.0359203.ref019","doi-asserted-by":"crossref","first-page":"144951","DOI":"10.1016\/j.gene.2020.144951","article-title":"Metagenomic study focusing on antibiotic resistance genes from the sediments of River Yamuna","volume":"758","author":"BK Das","year":"2020","journal-title":"Gene"},{"issue":"10","key":"pone.0359203.ref020","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1021\/es048468f","article-title":"Elevated microbial tolerance to metals and antibiotics in metal-contaminated industrial environments","volume":"39","author":"R Stepanauskas","year":"2005","journal-title":"Environ Sci Technol"},{"key":"pone.0359203.ref021","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1016\/j.scitotenv.2018.11.372","article-title":"Evidence for co-selection of antibiotic resistance genes and mobile genetic elements in metal polluted urban soils","volume":"656","author":"Y Zhao","year":"2019","journal-title":"Sci Total Environ"},{"issue":"4","key":"pone.0359203.ref022","doi-asserted-by":"crossref","DOI":"10.1093\/femsre\/fuae017","article-title":"Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria","volume":"48","author":"BF Gillieatt","year":"2024","journal-title":"FEMS Microbiol Rev"},{"key":"pone.0359203.ref023","doi-asserted-by":"crossref","unstructured":"Yulianto B, Oetari PS, Februhardi S, Putranto TWC, Soegianto A. Heavy metals (Cd, Pb, Cu, Zn) concentrations in edible bivalves harvested from Northern Coast of Central Java, Indonesia. 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American Society for Microbiology: Gram Stain Protocols. www.asmscience.org. 2020."},{"issue":"3","key":"pone.0359203.ref029","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/0038-0717(88)90014-4","article-title":"A direct extraction method to estimate soil microbial C: calibration in situ using microbial respiration and 14C labelled cells","volume":"20","author":"GP Sparling","year":"1988","journal-title":"Soil Biology and Biochemistry"},{"key":"pone.0359203.ref030","doi-asserted-by":"crossref","first-page":"158725","DOI":"10.1016\/j.scitotenv.2022.158725","article-title":"Microbiological analysis, antimicrobial activity, heavy-metals content and physico-chemical properties of Fijian mud pool samples","volume":"854","author":"A Pipite","year":"2023","journal-title":"Sci Total Environ"},{"key":"pone.0359203.ref031","doi-asserted-by":"crossref","DOI":"10.1128\/9781555817886","volume-title":"Antibiotics: actions, origins, resistance","author":"C Walsh","year":"2003","edition":"1"},{"key":"pone.0359203.ref032","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2023"},{"key":"pone.0359203.ref033","volume-title":"Fish and Fisheries Products Hazards and Controls Guidance","author":"FDA","year":"2001"},{"key":"pone.0359203.ref034","first-page":"1","article-title":"The streak plate protocol","author":"DS Katz","year":"2016","journal-title":"Microbe Library"},{"key":"pone.0359203.ref035","first-page":"3","article-title":"Rhodococcus equi: An emerging zoonotic pathogen","volume":"2","author":"M Pal","year":"2004","journal-title":"AVAS Ann Veterin Animal Sci"},{"issue":"5","key":"pone.0359203.ref036","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1111\/j.1574-6976.2009.00181.x","article-title":"Molecular and infection biology of the horse pathogen Rhodococcus equi","volume":"33","author":"K von Bargen","year":"2009","journal-title":"FEMS Microbiol 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Choi","year":"2013","journal-title":"Int J Syst Evol Microbiol"},{"key":"pone.0359203.ref043","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1099\/ijs.0.63383-0","article-title":"Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta","volume":"55","author":"MM S\u00e1nchez","year":"2005","journal-title":"Int J Syst Evol Microbiol"},{"key":"pone.0359203.ref044","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1099\/ijs.0.63265-0","article-title":"Microbacterium natoriense sp. nov., a novel D-aminoacylase-producing bacterium isolated from soil in Natori, Japan","volume":"55","author":"J Liu","year":"2005","journal-title":"Int J Syst Evol Microbiol"},{"issue":"2","key":"pone.0359203.ref045","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.01746-17","article-title":"Staphylococcus edaphicus sp. nov., isolated in Antarctica, harbors the mecC gene and genomic islands with a suspected role in adaptation to extreme environments","volume":"84","author":"R Pant\u016f\u010dek","year":"2018","journal-title":"Appl Environ Microbiol"},{"key":"pone.0359203.ref046","doi-asserted-by":"crossref","first-page":"967415","DOI":"10.3389\/fmicb.2022.967415","article-title":"Plant growth-promoting properties of Streptomyces spp. isolates and their impact on mung bean plantlets\u2019 rhizosphere microbiome","volume":"13","author":"N Nonthakaew","year":"2022","journal-title":"Front Microbiol"},{"issue":"6","key":"pone.0359203.ref047","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1007\/s12275-014-4071-7","article-title":"Paenibacillus cucumis sp. nov. isolated from greenhouse soil","volume":"52","author":"J-H Ahn","year":"2014","journal-title":"J Microbiol"},{"issue":"5","key":"pone.0359203.ref048","doi-asserted-by":"crossref","DOI":"10.1016\/j.cmi.2014.12.005","article-title":"Rhizobium pusense is the main human pathogen in the genus Agrobacterium\/Rhizobium","volume":"21","author":"F Aujoulat","year":"2015","journal-title":"Clinical Microbiol Infection"},{"issue":"8","key":"pone.0359203.ref049","first-page":"678","article-title":"Antibiotic susceptibility testing of isolated Bacillus thuringiensis from three soil types around Iligan City, Philippines","volume":"7","author":"JR Bautista","year":"2013","journal-title":"African J Microbiol Res"},{"key":"pone.0359203.ref050","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.nmni.2015.04.006","article-title":"Sphingobacterium multivorum: Case report and literature review","volume":"7","author":"F Barahona","year":"2015","journal-title":"New Microbes and New Infections"},{"issue":"3","key":"pone.0359203.ref051","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1111\/jam.12280","article-title":"Identification and virulence of Aeromonas dhakensis, Pseudomonas mosselii and Microbacterium paraoxydans isolated from Nile tilapia, Oreochromis niloticus, cultivated in Mexico","volume":"115","author":"SA Soto-Rodriguez","year":"2013","journal-title":"J Appl Microbiol"},{"issue":"4","key":"pone.0359203.ref052","doi-asserted-by":"crossref","first-page":"337","DOI":"10.4314\/ovj.v7i4.9","article-title":"Prevalence and antimicrobial resistance of Bacillus cereus isolated from beef products in Egypt","volume":"7","author":"R Shawish","year":"2017","journal-title":"Open Vet J"},{"key":"pone.0359203.ref053","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1099\/ijs.0.64639-0","article-title":"Exiguobacterium profundum sp. nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent","volume":"57","author":"S Crapart","year":"2007","journal-title":"Int J Syst Evol Microbiol"},{"key":"pone.0359203.ref054","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1099\/ijs.0.64508-0","article-title":"Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India","volume":"56","author":"P Chaturvedi","year":"2006","journal-title":"Int J Syst Evol Microbiol"},{"issue":"8","key":"pone.0359203.ref055","article-title":"Endophytic bacterium Bacillus aryabhattai induces novel transcriptomic changes to stimulate plant growth","volume":"17","author":"H Xu","year":"2022","journal-title":"PLoS One"},{"key":"pone.0359203.ref056","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1099\/00207713-48-3-1031","article-title":"Bacillus pseudomycoides sp. nov","author":"LK Nakamura","year":"1998","journal-title":"Int J Syst Bacteriol"},{"issue":"2","key":"pone.0359203.ref057","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.krcp.2013.04.004","article-title":"Successful antibiotic treatment of Pseudomonas stutzeri-induced peritonitis without peritoneal dialysis catheter removal in continuous ambulatory peritoneal dialysis","volume":"32","author":"SW Park","year":"2013","journal-title":"Kidney Res Clin Pract"},{"issue":"3","key":"pone.0359203.ref058","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1007\/s10482-013-0097-9","article-title":"Fictibacillus enclensis sp. nov., isolated from marine sediment","volume":"105","author":"SG Dastager","year":"2014","journal-title":"Antonie Van Leeuwenhoek"},{"issue":"1","key":"pone.0359203.ref059","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.marpol.2008.04.002","article-title":"Planning the use of fish for food security in the Pacific","volume":"33","author":"JD Bell","year":"2009","journal-title":"Marine Policy"},{"key":"pone.0359203.ref060","volume-title":"Tuna: a key economic resource in the Pacific Islands","author":"RD Gillett","year":"2001"},{"key":"pone.0359203.ref061","unstructured":"Nawai J, Gusyev MA, Hasegawa A, Takeuchi K, Nawai J, Gusyev MA. Flood and drought assessment with dam infrastructure: A case study of the Ba River basin, Fiji. In: MODSIM2015, 21st International Congress on Modelling and Simulation, 2015."},{"key":"pone.0359203.ref062","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1108\/S2040-7262(2013)0000014010","article-title":"The role of social capital in post-flood response and recovery among downstream communities of the Ba River, Western Viti Levu, Fiji Islands","volume-title":"Risks and conflicts: Local responses to natural disasters","author":"O Yila","year":"2013"},{"key":"pone.0359203.ref063","volume-title":"The microbial profiling of the Qawa River using molecular methods","author":"A Bainivalu","year":"2016"},{"issue":"1","key":"pone.0359203.ref064","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1071\/SP05009","article-title":"Bacteriology of the fresh water bivalve clam Batissa violacea (Kai) sold in the Suva market","volume":"23","author":"AAM Hatha","year":"2005","journal-title":"The South Pacific Journal of Natural and Applied Sciences"},{"key":"pone.0359203.ref065","article-title":"Depuration of a Freshwater Clam (Batissa Violacea) from Rewa River in Fiji Using a Bio-Filter Set-Up in Closed and Open Water Circulatory System","author":"AA Singh","year":"2021","journal-title":"Nova Biotechnol Chim"},{"issue":"2","key":"pone.0359203.ref066","doi-asserted-by":"crossref","first-page":"409","DOI":"10.2983\/035.033.0209","article-title":"Depuration of bacterial populations in the Indian backwater oyster Crassostrea madrasensis (Preston, 1916): effects on surface and bottom held oysters","volume":"33","author":"S Chinnadurai","year":"2014","journal-title":"Journal of Shellfish Research"},{"issue":"1","key":"pone.0359203.ref067","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s13197-011-0550-6","article-title":"Combined effect of lime (Citrus aurantitolia) and drying on reducing bacteria of public health significance in Edible Oyster (Crassostrea madrasensis)","volume":"50","author":"F Hassan","year":"2013","journal-title":"J Food Sci Technol"},{"issue":"16","key":"pone.0359203.ref068","doi-asserted-by":"crossref","first-page":"3978","DOI":"10.1016\/S0043-1354(03)00301-4","article-title":"Fecal indicator bacteria are abundant in wet sand at freshwater beaches","volume":"37","author":"E Wheeler Alm","year":"2003","journal-title":"Water Res"},{"issue":"9","key":"pone.0359203.ref069","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1016\/0043-1354(96)00079-6","article-title":"Relationships between indicators, pathogens and water quality in an estuarine system","volume":"30","author":"CM Ferguson","year":"1996","journal-title":"Water Research"},{"issue":"3","key":"pone.0359203.ref070","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1016\/j.jenvman.2008.08.001","article-title":"Size distributions of manure particles released under simulated rainfall","volume":"90","author":"YA Pachepsky","year":"2009","journal-title":"J Environ 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Tenaillon","year":"2010","journal-title":"Nat Rev Microbiol"},{"issue":"1","key":"pone.0359203.ref074","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41572-018-0005-8","article-title":"Vibrio spp. infections","volume":"4","author":"C Baker-Austin","year":"2018","journal-title":"Nature Reviews Disease Primers"},{"issue":"3","key":"pone.0359203.ref075","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1017\/S0950268899003714","article-title":"Vibrio gastroenteritis in the US Gulf of Mexico region: the role of raw oysters","volume":"124","author":"SF Altekruse","year":"2000","journal-title":"Epidemiol Infect"},{"key":"pone.0359203.ref076","doi-asserted-by":"crossref","first-page":"106697","DOI":"10.1016\/j.foodcont.2019.06.023","article-title":"Distribution of Vibrio species isolated from bivalves and bivalve culture environments along the Gyeongnam coast in Korea: Virulence and antimicrobial resistance of Vibrio parahaemolyticus isolates","volume":"106","author":"JS Mok","year":"2019","journal-title":"Food Control"},{"key":"pone.0359203.ref077","first-page":"5646480","article-title":"Riverbed Sediments as Reservoirs of Multiple Vibrio cholerae Virulence-Associated Genes: A Potential Trigger for Cholera Outbreaks in Developing Countries","volume":"2017","author":"ALK Abia","year":"2017","journal-title":"J Environ Public Health"},{"key":"pone.0359203.ref078","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/B978-0-12-803678-5.00434-3","volume-title":"Streptococcal Diseases. 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Most Probable Number (MPN) European Union reference method for enumeration of Escherichia coli in marine bivalves","volume":"131","author":"DH Grevskott","year":"2016","journal-title":"J Microbiol Methods"},{"key":"pone.0359203.ref088","doi-asserted-by":"crossref","first-page":"134377","DOI":"10.1016\/j.jhazmat.2024.134377","article-title":"Metagenomic landscape of sediments of river Ganga reveals microbial diversity, potential plastic and xenobiotic degradation enzymes","volume":"471","author":"AK Rout","year":"2024","journal-title":"J Hazard Mater"},{"key":"pone.0359203.ref089","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1016\/j.scitotenv.2018.09.314","article-title":"Assessing microbial contamination and antibiotic resistant bacteria using zebra mussels (Dreissena polymorpha)","volume":"650","author":"MA Bighiu","year":"2019","journal-title":"Sci Total Environ"},{"key":"pone.0359203.ref090","article-title":"Marine bivalve mollusks as possible indicators of 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