{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:09:11Z","timestamp":1790273351928,"version":"4.1.0"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K08605"],"award-info":[{"award-number":["24K08605"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    We investigated the behavioral responses of the slime mold\n                    <jats:italic>Physarum polycephalum<\/jats:italic>\n                    to\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    expressing human small GTPases to elucidate how internalized proteins affect its actomyosin-based motility machinery. We found that prey bacteria expressing hKRAS WT or hRhoA triggered a deterministic \u201cconflict\u201d behavior, in which\n                    <jats:italic>P. polycephalum<\/jats:italic>\n                    failed to complete ingestion despite physical contact. Notably, the oncogenic hKRAS G12C mutation induced a transition to stochastic switching among predation, conflict, and sampling-mediated rejection, whereas hRac1 expression showed a tendency toward diversified behavioral outcomes. To explain this, we propose a working model centered on molecular jamming or local perturbation of intracellular oscillatory dynamics, in which non-prenylated G-domains act as decoys that decouple the contractile machinery from leading-edge actin dynamics. This behavioral stochasticity likely reflects physical noise arising from the specific conformational states and functional heterogeneity of the expressed proteins. Our results suggest that\n                    <jats:italic>P. polycephalum<\/jats:italic>\n                    functions as a sensitive biological sensor of the biophysical properties of prey proteins, providing a unique platform for exploring the co-evolutionary arms race and molecular survival strategies between bacteria and amoeboid predators.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359275","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:31:36Z","timestamp":1790271096000},"page":"e0359275","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bacterial expression of human small GTPases induces stochastic predatory responses and motility interference in Physarum polycephalum"],"prefix":"10.1371","volume":"21","author":[{"given":"Rui","family":"Saiki","sequence":"first","affiliation":[{"name":"Nippon Institute of Technology","place":["Saitama, Japan"],"department":["Department of Applied Chemistry, Faculty of Fundamental Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rion","family":"Iimura","sequence":"additional","affiliation":[{"name":"Tochigi Prefectural Oyama High School","place":["Oyama, Tochigi, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Ichimura","sequence":"additional","affiliation":[{"name":"Nippon Institute of Technology","place":["Saitama, Japan"],"department":["Department of Innovative System Engineering, Faculty of Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Osada","sequence":"additional","affiliation":[{"name":"RIKEN","place":["Saitama, Japan"],"department":["Glycometabolic Biochemistry Laboratory"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0715-5040","authenticated-orcid":true,"given":"Ken-Ichi","family":"Sano","sequence":"additional","affiliation":[{"name":"Nippon Institute of Technology","place":["Saitama, Japan"],"department":["Department of Applied Chemistry, Faculty of Fundamental Engineering"]},{"name":"Nippon Institute of Technology","place":["Saitama, Japan"],"department":["Department of Innovative System Engineering, Faculty of Engineering"]},{"name":"Nippon Institute of Technology","place":["Saitama, Japan"],"department":["Department of Sustainable Biological Chemistry, Faculty of Fundamental Engineering"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"pone.0359275.ref001","volume-title":"Biology of the Myxomycetes","author":"WD 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