{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:59:27Z","timestamp":1790704767207,"version":"4.1.0"},"reference-count":39,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["26-22607K"],"award-info":[{"award-number":["26-22607K"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Heterotrimeric G proteins are key signal transducers in all eukaryotic cells. Anchored to the inner leaflet of the plasma membrane, they transmit and amplify extracellular chemical and physical signals downstream of G protein-coupled receptors. Despite numerous available studies, many biophysical aspects that regulate G protein signaling, including membrane mobility, remain poorly understood. Here, using single-molecule imaging, we show that different subtypes of heterotrimeric G proteins containing G\u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u03b3\n                    <jats:sub>2<\/jats:sub>\n                    subunits exhibit high diversity in their membrane mobility. We demonstrate that the nature of the G\u03b1 subunit plays a major role in defining the mobility of such heterotrimers. Our results indicate that heterotrimers containing G\u03b1\n                    <jats:sub>12<\/jats:sub>\n                    and G\u03b1\n                    <jats:sub>13<\/jats:sub>\n                    subunits exhibit markedly reduced mobility compared to those containing G\u03b1\n                    <jats:sub>i\/o<\/jats:sub>\n                    and G\u03b1\n                    <jats:sub>s<\/jats:sub>\n                    subunits. These findings identify subtype-specific lateral membrane mobility of G proteins as a factor that can affect their signaling dynamics in living cells.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359304","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:29:50Z","timestamp":1790702990000},"page":"e0359304","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Heterotrimeric G proteins containing G\u03b21\u03b32 subunits exhibit subtype-specific mobility differences in live cells"],"prefix":"10.1371","volume":"21","author":[{"given":"Ond\u0159ej","family":"Kuchynka","sequence":"first","affiliation":[{"name":"University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"],"department":["Faculty of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei","family":"Kovalchuk","sequence":"additional","affiliation":[{"name":"University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"],"department":["Faculty of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia","family":"Nu\u00dfbaumer","sequence":"additional","affiliation":[{"name":"University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"],"department":["Faculty of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Sviridova","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Biology Centre of the Czech Academy of Sciences","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1\u0161","family":"Fessl","sequence":"additional","affiliation":[{"name":"University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"],"department":["Faculty of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1980-2930","authenticated-orcid":true,"given":"Alexey","family":"Bondar","sequence":"additional","affiliation":[{"name":"University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"],"department":["Faculty of Science"]},{"name":"Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences","place":["\u010cesk\u00e9 Bud\u011bjovice, Czechia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"5","key":"pone.0359304.ref001","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.3390\/cells10051228","article-title":"The role of G protein-coupled receptors (GPCRs) and calcium signaling in schizophrenia. 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