{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:08:40Z","timestamp":1790273320624,"version":"4.1.0"},"reference-count":35,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      Dupuytren\u2019s disease is a fibroproliferative disorder that is characterized by collagen-rich cords that cause progressive flexion contractures. Collagenase from\n                      <jats:italic>Clostridium histolyticum<\/jats:italic>\n                      is an established nonsurgical therapy. Herein, we evaluated whether a recombinant form of collagenase G (ColG), expressed in\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      , weakens Dupuytren\u2019s cords ex vivo.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Cords from Dupuytren\u2019s disease patients were excised during surgery. Using a 30G needle, 0.5 mL of 4 mg\/ml ColG or phosphate buffer Saline (PBS) was injected into the cord center. Samples were incubated in saline at 37 \u00b0C and mounted via size-1 Prolene Krackow sutures on an Instron tensile testing system. Force vs. displacement curves were recorded.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Six cords from five patients were tested (ColG n\u2009=\u20094; PBS n\u2009=\u20092). Representative force\u2013displacement curves showed a reduced slope and lower peak force prior to rupture in ColG-treated samples compared with vehicle. The mean maximum rupture force was lower with ColG than with PBS (0.047 kN vs 0.069 kN), corresponding to ~32% reduction in the force required to tear the cord in this series.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Recombinant ColG weakens the tensile strength of Dupuytren\u2019s cords ex vivo, consistent with potent collagenolytic activity. These proof-of-concept pilot data support further studies to define dose\u2013response, optimize formulation and exposure time, and evaluate safety and clinical performance relative to current collagenase-based treatments.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0359330","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:38:13Z","timestamp":1790271493000},"page":"e0359330","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dupuytren\u2019s disease cords are weakened ex vivo by injection of recombinant collagenase G \u2013 A proof-of-concept study"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1032-7280","authenticated-orcid":true,"given":"Guy","family":"Rubin","sequence":"first","affiliation":[{"name":"Emek Medical Center","place":["Afula, Israel"],"department":["Orthopedic Department"]},{"name":"Technion","place":["Haifa, Israel"],"department":["Faculty of Medicine"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Rothem","sequence":"additional","affiliation":[{"name":"Emek Medical Center","place":["Afula, Israel"],"department":["Orthopedic Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Tohar","sequence":"additional","affiliation":[{"name":"Tel Aviv University","place":["Tel Aviv, Israel"],"department":["Department of Oral Biology, Goldschleger School of Dental Medicine, Gray Faculty of Medical and Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maayan","family":"Gal","sequence":"additional","affiliation":[{"name":"Tel Aviv University","place":["Tel Aviv, Israel"],"department":["Department of Oral Biology, Goldschleger School of Dental Medicine, Gray Faculty of Medical and Health Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"issue":"5","key":"pone.0359330.ref001","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1016\/j.jhsa.2005.03.005","article-title":"A retrospective review of the management of Dupuytren\u2019s nodules","volume":"30","author":"RM Reilly","year":"2005","journal-title":"J Hand Surg Am"},{"issue":"3","key":"pone.0359330.ref002","first-page":"291","article-title":"Epidemiology of Dupuytren\u2019s disease: clinical, serological, and social assessment. 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