{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:53:28Z","timestamp":1792788808643,"version":"4.5.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control","award":["HPKL2023005"],"award-info":[{"award-number":["HPKL2023005"]}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2026JJ80918"],"award-info":[{"award-number":["2026JJ80918"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Innovation and entrepreneurship fund for college students of Hunan Normal University","award":["2024054"],"award-info":[{"award-number":["2024054"]}],"id":[{"id":"https:\/\/ror.org\/053w1zy07","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>This study aimed to evaluate the protective effects of Dioscin in an age-related macular degeneration (AMD) -like model and to explore its potential therapeutic mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The therapeutic potential of Dioscin for AMD was investigated using D-galactose-induced senescence models in ARPE-19 cells (an in vitro preliminary screening model) and C57BL\/6J mice using RT-qPCR, western blotting, immunofluorescence, and SA-\u03b2-gal staining. Bioinformatic analysis and molecular docking identified TGF-\u03b22 as a computationally predicted potential target of Dioscin in AMD. Key mechanisms involving TGF-\u03b22 and the PI3K\/Akt\/\u03b2-catenin signaling pathway were assessed in vitro using RT-qPCR, western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In D-galactose-induced senescence models, Dioscin significantly alleviated RPE senescence and EMT. Single-cell transcriptome analysis revealed that TGF-\u03b22 is highly expressed in choroidal vascular endothelial cells of AMD patients. Mechanistically, computational predictions (molecular docking and dynamics simulations) identified TGF-\u03b22 as a computationally predicted potential target of Dioscin, and experimental data showed that Dioscin suppressed D-galactose-induced activation of the TGF-\u03b22\/PI3K\/Akt\/\u03b2-catenin pathway in RPE cells, suggesting a potential paracrine regulatory mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Dioscin alleviates RPE senescence and EMT through suppression of the TGF-\u03b22\/PI3K\/Akt\/\u03b2-catenin pathway. Single-cell transcriptomics reveals that TGF-\u03b22 is specifically upregulated in choroidal vascular endothelial cells in AMD, suggesting a paracrine mechanism linking vascular pathology to RPE dysfunction. These findings suggest that Dioscin may have potential value in mitigating RPE senescence and EMT in an AMD\u2011like model, although further validation in more clinically relevant AMD models is required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0359423","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:35:16Z","timestamp":1790616916000},"page":"e0359423","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dioscin attenuates D\u2011galactose\u2011induced retinal pigment epithelium senescence and epithelial\u2013mesenchymal transition via the TGF\u2011\u03b22\/PI3K\/Akt\/\u03b2\u2011catenin pathway"],"prefix":"10.1371","volume":"21","author":[{"given":"Qi","family":"Ouyang","sequence":"first","affiliation":[{"name":"The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan 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