{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:49:03Z","timestamp":1791982143273,"version":"4.4.0"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Inositol tetrakisphosphates kinase (\n                    <jats:italic>ITPK<\/jats:italic>\n                    ) plays a vital role in regulating cellular responses to abiotic stress, plant hormone signaling and various other aspects of plant growth and development. Additionally, it is also involved in phosphorous, inositol phosphate metabolism and synthesize phytic acid which is the main phosphorous storage form in seeds. While essential for plant physiology, high phytic acid levels reduce the bioavailability of minerals such as Fe and Zn, which impact nutritional quality negatively. Despite its importance, the\n                    <jats:italic>ITPK<\/jats:italic>\n                    gene family remains largely unexplored in bread wheat. In this study, we conducted a comprehensive genome-wide analysis, including phylogenetic analysis and expression profiling, of the\n                    <jats:italic>ITPK<\/jats:italic>\n                    gene family in wheat. We systematically examined gene structure, chromosomal distribution, motif and domain conservation, promoter\u00a0analysis, synteny\u00a0analysis, protein modeling and protein-protein interaction of\n                    <jats:italic>ITPKs<\/jats:italic>\n                    . A total of 15\n                    <jats:italic>ITPK<\/jats:italic>\n                    genes have been identified in wheat (\n                    <jats:italic>T. aestivum<\/jats:italic>\n                    ). The\n                    <jats:italic>TaITPK<\/jats:italic>\n                    genes were distributed on chromosome number 1, 4, 5 and 7 in all the three genomes A, B and D. Comparative phylogenetic analysis resolved four major groups, with wheat\n                    <jats:italic>ITPK<\/jats:italic>\n                    sequences represented in Groups I, III, and IV, whereas Group II contained no wheat sequence. The conserved domain and motif analysis showed the presence of Ins134_P3_kin domain at N-terminus and CPase_L_D2 in C-terminus of protein. String based protein interaction analysis suggested\n                    <jats:italic>ITPK<\/jats:italic>\n                    genes interact with Inositol polyphosphate multi kinase (\n                    <jats:italic>IPK1<\/jats:italic>\n                    ) and Inositol-pentakisphosphate 2 kinase (\n                    <jats:italic>IPK2<\/jats:italic>\n                    ) which were also involve in synthesis of phyti acid. Furthermore, in-silico expression analysis of\n                    <jats:italic>ITPK<\/jats:italic>\n                    genes revealed their preferential tissue-specific and growth stage expression, especially after anthesis of wheat. Preliminary qRT-PCR profiling of selected\n                    <jats:italic>TaITPK<\/jats:italic>\n                    genes revealed treatment-dependent relative expression patterns under FeSO\n                    <jats:sub>4<\/jats:sub>\n                    and ZnSO\n                    <jats:sub>4<\/jats:sub>\n                    supplementation. The current study has identified 15\n                    <jats:italic>ITPK<\/jats:italic>\n                    genes present in hexaploid wheat genome. Their evolutionary, structural, and expression analyses provide a foundation for further functional characterization of this gene family in wheat.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359528","type":"journal-article","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:26:41Z","timestamp":1790702801000},"page":"e0359528","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genome wide identification and analysis of inositol tetrakisphosphate kinase (ITPK) gene family in Triticum aestivum L."],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8710-2299","authenticated-orcid":true,"given":"Fatima","family":"Umar","sequence":"first","affiliation":[{"name":"University of Agriculture","place":["Faisalabad, Pakistan"],"department":["Department of Plant Breeding and Genetics, Faculty of Agriculture"]},{"name":"Centre of Advanced Studies in Agriculture and Food Security, University of Agriculture","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rizwana","family":"Maqbool","sequence":"additional","affiliation":[{"name":"University of Agriculture","place":["Faisalabad, Pakistan"],"department":["Department of Plant Breeding and Genetics, Faculty of Agriculture"]},{"name":"Centre of Advanced Studies in Agriculture and Food Security, University of Agriculture","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Ahsan","family":"Khan","sequence":"additional","affiliation":[{"name":"University of Agriculture","place":["Faisalabad, Pakistan"],"department":["Department of Plant Breeding and Genetics, Faculty of Agriculture"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bushra","family":"Sadia","sequence":"additional","affiliation":[{"name":"Centre of Agricultural Biochemistry and Biotechnology, University of Agriculture","place":["Faisalabad, Pakistan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,9,29]]},"reference":[{"issue":"3","key":"pone.0359528.ref001","doi-asserted-by":"crossref","first-page":"652","DOI":"10.3390\/molecules30030652","article-title":"Towards Improved Bioavailability of Cereal Inositol Phosphates, Myo-Inositol and Phenolic Acids","volume":"30","author":"K \u017by\u0142a","year":"2025","journal-title":"Molecules"},{"issue":"6","key":"pone.0359528.ref002","first-page":"1554","article-title":"A review phytic acid: as antinutrient or nutraceutical","volume":"6","author":"J Nissar","year":"2017","journal-title":"J Pharmacogn Phytochem"},{"issue":"1","key":"pone.0359528.ref003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/pbr.12658","article-title":"Zinc biofortification of maize (Zea mays L.): Status and challenges","volume":"138","author":"MA Maqbool","year":"2018","journal-title":"Plant 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