{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:50:40Z","timestamp":1791226240233,"version":"4.1.0"},"reference-count":64,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Centre of Sciences","award":["2018\/31\/B\/NZ9\/01548"],"award-info":[{"award-number":["2018\/31\/B\/NZ9\/01548"]}]},{"name":"Institute of Dendrology, Polish Academy of Sciences","id":[{"id":"https:\/\/ror.org\/01dr6c206","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Spanish Ministry of Science and Innovation (MICINN) and the European Regional Development Fund","award":["PID2021-125913NB-C22"],"award-info":[{"award-number":["PID2021-125913NB-C22"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    Seed viability and longevity underpin forest regeneration under changing environmental conditions. European beech (\n                    <jats:italic>Fagus sylvatica<\/jats:italic>\n                    L.) produces intermediate seeds that gradually lose viability during storage, largely through oxidative stress, which makes redox homeostasis central to their longevity. Here we investigated the role of thioredoxin\n                    <jats:italic>h1<\/jats:italic>\n                    (TRX\n                    <jats:italic>h1<\/jats:italic>\n                    ), a cytosolic thiol-disulfide oxidoreductase, in the embryonic axes of beech seeds. Using a His-tagged monocysteinic (Cys to Ser) trapping mutant of TRX\n                    <jats:italic>h1<\/jats:italic>\n                    combined with data-dependent acquisition (DDA) proteomics, we identified 161 putative TRX\n                    <jats:italic>h1<\/jats:italic>\n                    target proteins, which fall into functional categories related to genetic information processing, metabolism, environmental and cellular signaling, and protein homeostasis. Western blotting confirmed that target capture proceeded through DTT-reducible mixed-disulfide complexes, supporting a redox-based interaction. The targets include heat shock proteins (HSP70 and HSP90) and other chaperones, calcium-binding proteins (calmodulin and calreticulin), a methyl-CpG-binding protein, glycolytic and tricarboxylic acid (TCA) cycle enzymes, and antioxidant enzymes such as superoxide dismutase, glutathione peroxidase and glutaredoxin. To link these targets to seed physiology, we quantified the thiol redox status of fresh and long-term stored seeds: total thiols were approximately 2.2-fold lower in stored than in fresh axes (21.0\u2009\u00b1\u20090.5 vs 45.9\u2009\u00b1\u20091.3 nmol GSH equivalents per axis), indicating that the reduced thiol pool, the substrate of the TRX\n                    <jats:italic>h1<\/jats:italic>\n                    system, becomes depleted and more oxidized during storage. Together, these results suggest that TRX\n                    <jats:italic>h1<\/jats:italic>\n                    -dependent redox maintenance may contribute to redox homeostasis, energy metabolism and protein stability in beech embryonic axes, and provide a basis for improving seed storage and conservation strategies.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0359622","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:53:31Z","timestamp":1791222811000},"page":"e0359622","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Thioredoxin h1-targeted proteins in European beech (Fagus sylvatica L.): Insights into metabolic control, stress response, and seed longevity"],"prefix":"10.1371","volume":"21","author":[{"given":"Ewelina","family":"Ratajczak","sequence":"first","affiliation":[{"name":"Institute of Dendrology, Polish Academy of Sciences","place":["Parkowa, K\u00f3rnik, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra M.","family":"Staszak","sequence":"additional","affiliation":[{"name":"University of Bia\u0142ystok","place":["Cio\u0142kowskiego, Bia\u0142ystok, Poland"],"department":["Laboratory of Plant Physiology, Department of Plant Biology and Ecology, Faculty of Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Vargas","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas (CSIC)","place":["Granada, Spain"],"department":["Department of Stress, Development and Signaling in Plants"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Sahrawy","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas (CSIC)","place":["Granada, Spain"],"department":["Department of Stress, Development and Signaling in Plants"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio J.","family":"Serrato","sequence":"additional","affiliation":[{"name":"Estaci\u00f3n Experimental del Zaid\u00edn, Consejo Superior de Investigaciones Cient\u00edficas (CSIC)","place":["Granada, Spain"],"department":["Department of Stress, Development and Signaling in Plants"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2369-2551","authenticated-orcid":true,"given":"Hanna","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Institute of Dendrology, Polish Academy of Sciences","place":["Parkowa, K\u00f3rnik, Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"issue":"9","key":"pone.0359622.ref001","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1890\/04-1956","article-title":"Performance trade-offs among tropical tree seedlings in contrasting microhabitats","volume":"86","author":"C Baraloto","year":"2005","journal-title":"Ecology"},{"issue":"1","key":"pone.0359622.ref002","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/nph.17682","article-title":"How will global change affect plant reproduction? A framework for mast seeding trends","volume":"234","author":"M Bogdziewicz","year":"2022","journal-title":"New Phytol"},{"issue":"9","key":"pone.0359622.ref003","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0218421","article-title":"Lower dormancy with rapid germination is an important strategy for seeds in an arid zone with unpredictable rainfall","volume":"14","author":"C Duncan","year":"2019","journal-title":"PLoS One"},{"issue":"2","key":"pone.0359622.ref004","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1038\/s41477-020-0592-8","article-title":"Climate warming disrupts mast seeding and its fitness benefits in European beech","volume":"6","author":"M Bogdziewicz","year":"2020","journal-title":"Nat Plants"},{"issue":"3","key":"pone.0359622.ref005","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1111\/1365-2745.13337","article-title":"Decadal changes in masting behaviour of oak trees with rising temperature","volume":"108","author":"M Shibata","year":"2020","journal-title":"J Ecol"},{"issue":"1","key":"pone.0359622.ref006","article-title":"Advances in seed conservation of wild plant species: a review of recent research","volume":"1","author":"FR Hay","year":"2013","journal-title":"Conserv Physiol"},{"issue":"4","key":"pone.0359622.ref007","first-page":"827","article-title":"Late seed maturation: drying without dying","volume":"68","author":"O Leprince","year":"2017","journal-title":"J Exp Bot"},{"issue":"11","key":"pone.0359622.ref008","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1016\/j.bbapap.2009.07.013","article-title":"A comparative proteomic analysis of rice seedlings under various high-temperature stresses","volume":"1794","author":"F Han","year":"2009","journal-title":"Biochim Biophys Acta"},{"issue":"2","key":"pone.0359622.ref009","doi-asserted-by":"crossref","first-page":"158","DOI":"10.14720\/aas.2014.103.2.1","article-title":"Effects of seed size and aging on field performance of lentil (Lens culinaris Medik.) under different irrigation treatments","volume":"103","author":"K Ghassemi-Golezani","year":"2014","journal-title":"Acta Agric Sloven"},{"key":"pone.0359622.ref010","doi-asserted-by":"crossref","first-page":"1","DOI":"10.12657\/denbio.091.001","article-title":"Tree-ring formation dynamics in Fagus sylvatica and Quercus petraea in a dry and a wet year","volume":"91","author":"R Pucha\u0142ka","year":"2024","journal-title":"Dendrobiology"},{"key":"pone.0359622.ref011","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1111\/gcb.13925","article-title":"How much does climate change threaten European forest tree species distributions?","volume":"24","author":"MK Dyderski","year":"2018","journal-title":"Global Change Biol"},{"issue":"1","key":"pone.0359622.ref012","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1079\/SSR200195","article-title":"Seed storage behaviour of Fagus sylvatica and Fagus crenata","volume":"12","author":"P Le\u00f3n-Lobos","year":"2002","journal-title":"Seed Sci Res"},{"issue":"1","key":"pone.0359622.ref013","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/S0304-4165(03)00046-1","article-title":"Water and lipid relations in beech (Fagus sylvatica L.) seeds and its effect on storage behaviour","volume":"1621","author":"S Pukacka","year":"2003","journal-title":"Biochim Biophys Acta"},{"issue":"1","key":"pone.0359622.ref014","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s10725-010-9492-7","article-title":"Ascorbate and glutathione metabolism during development and desiccation of beech (Fagus sylvatica L.) seeds","volume":"62","author":"S Pukacka","year":"2010","journal-title":"Plant Growth Regul"},{"key":"pone.0359622.ref015","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jplph.2014.08.021","article-title":"The production, localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech (Fagus sylvatica L.) seeds","volume":"174","author":"E Ratajczak","year":"2015","journal-title":"J Plant Physiol"},{"key":"pone.0359622.ref016","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.jplph.2019.06.002","article-title":"Regulation of thiol metabolism as a factor that influences the development and storage capacity of beech seeds","volume":"239","author":"E Ratajczak","year":"2019","journal-title":"J Plant Physiol"},{"issue":"3","key":"pone.0359622.ref017","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1023\/A:1010697331184","article-title":"Characterization of two thioredoxins h with predominant localization in the nucleus of aleurone and scutellum cells of germinating wheat seeds","volume":"46","author":"AJ Serrato","year":"2001","journal-title":"Plant Mol Biol"},{"key":"pone.0359622.ref018","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1042\/bj20020103","article-title":"Cloning of thioredoxin h reductase and characterization of the thioredoxin reductase-thioredoxin h system from wheat","volume":"367","author":"AJ Serrato","year":"2002","journal-title":"Biochem J"},{"issue":"1","key":"pone.0359622.ref019","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1111\/j.1365-313X.2008.03675.x","article-title":"An antioxidant redox system in the nucleus of wheat seed cells suffering oxidative stress","volume":"57","author":"P Pulido","year":"2009","journal-title":"Plant J"},{"issue":"3","key":"pone.0359622.ref020","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1007\/s00425-003-1009-4","article-title":"Type-h thioredoxins accumulate in the nucleus of developing wheat seed tissues suffering oxidative stress","volume":"217","author":"AJ Serrato","year":"2003","journal-title":"Planta"},{"key":"pone.0359622.ref021","doi-asserted-by":"crossref","first-page":"1430695","DOI":"10.3389\/fpls.2024.1430695","article-title":"Redox dynamics in seeds of Acer spp: unraveling adaptation strategies of different seed categories","volume":"15","author":"H Fuchs","year":"2024","journal-title":"Front Plant Sci"},{"key":"pone.0359622.ref022","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"},{"issue":"3","key":"pone.0359622.ref023","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1093\/jxb\/erab452","article-title":"Arabidopsis fibrillin 1-2 subfamily members exert their functions via specific protein-protein interactions","volume":"73","author":"D Torres-Romero","year":"2022","journal-title":"J Exp Bot"},{"key":"pone.0359622.ref024","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2025"},{"key":"pone.0359622.ref025","doi-asserted-by":"crossref","first-page":"706907","DOI":"10.3389\/fgene.2021.706907","article-title":"ggVennDiagram: An Intuitive, Easy-to-Use, and Highly Customizable R Package to Generate Venn Diagram","volume":"12","author":"C-H Gao","year":"2021","journal-title":"Frontiers in Genetics"},{"issue":"1","key":"pone.0359622.ref026","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/pcp\/pcy194","article-title":"The mitochondrial thioredoxin system contributes to the metabolic responses under drought episodes in arabidopsis","volume":"60","author":"P da Fonseca-Pereira","year":"2019","journal-title":"Plant Cell Physiol"},{"issue":"8","key":"pone.0359622.ref027","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1073\/pnas.0308583101","article-title":"Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria","volume":"101","author":"Y Balmer","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"pone.0359622.ref028","doi-asserted-by":"crossref","first-page":"3759","DOI":"10.1073\/pnas.0308701101","article-title":"Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli","volume":"101","author":"JK Kumar","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"pone.0359622.ref029","doi-asserted-by":"crossref","first-page":"323","DOI":"10.5423\/PPJ.RW.08.2015.0150","article-title":"Heat shock proteins: a review of the molecular chaperones for plant immunity","volume":"31","author":"C-J Park","year":"2015","journal-title":"Plant Pathol J"},{"issue":"12","key":"pone.0359622.ref030","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/BF02172188","article-title":"A new puffing pattern induced by temperature shock and DNP in drosophila","volume":"18","author":"F Ritossa","year":"1962","journal-title":"Experientia"},{"key":"pone.0359622.ref031","doi-asserted-by":"crossref","first-page":"386351","DOI":"10.1155\/2014\/386351","article-title":"Decreased thioredoxin-1 and increased HSP90 expression in skeletal muscle in subjects with type 2 diabetes or impaired glucose tolerance","volume":"2014","author":"M Venoj\u00e4rvi","year":"2014","journal-title":"Biomed Res Int"},{"key":"pone.0359622.ref032","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.toxicon.2017.10.028","article-title":"Hsp90 and thioredoxin-thioredoxin reductase enable the catalytic activity of clostridial neurotoxins inside nerve terminals","volume":"147","author":"M Pirazzini","year":"2018","journal-title":"Toxicon"},{"issue":"1","key":"pone.0359622.ref033","doi-asserted-by":"crossref","first-page":"115","DOI":"10.4062\/biomolther.2023.104","article-title":"Contribution of HSP90 cleavage to the cytotoxic effect of suberoylanilide hydroxamic acid in vivo and the involvement of TXNIP in HSP90 cleavage","volume":"32","author":"S Park","year":"2024","journal-title":"Biomol Ther (Seoul)"},{"key":"pone.0359622.ref034","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1074\/mcp.M114.040931","article-title":"Identification of novel nuclear targets of human thioredoxin 1*","volume":"13","author":"C Wu","year":"2014","journal-title":"Mol Cell Prot"},{"issue":"6","key":"pone.0359622.ref035","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.1007\/s00299-022-02857-y","article-title":"J-like protein family of Arabidopsis thaliana: the enigmatic cousins of J-domain proteins","volume":"41","author":"C Tamadaddi","year":"2022","journal-title":"Plant Cell Rep"},{"issue":"10","key":"pone.0359622.ref036","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1007\/PL00012490","article-title":"Nascent-polypeptide-associated complex","volume":"59","author":"S Rospert","year":"2002","journal-title":"Cell Mol Life Sci"},{"key":"pone.0359622.ref037","doi-asserted-by":"crossref","first-page":"610052","DOI":"10.3389\/fpls.2020.610052","article-title":"The Arabidopsis protein disulfide isomerase subfamily M isoform, PDI9, localizes to the endoplasmic reticulum and influences pollen viability and proper formation of the pollen exine during heat stress","volume":"11","author":"E Feldeverd","year":"2020","journal-title":"Front Plant Sci"},{"issue":"2","key":"pone.0359622.ref038","doi-asserted-by":"crossref","first-page":"167404","DOI":"10.1016\/j.jmb.2021.167404","article-title":"Family-wide characterization of methylated DNA binding ability of arabidopsis MBDs","volume":"434","author":"Z Wu","year":"2022","journal-title":"J Mol Biol"},{"issue":"1","key":"pone.0359622.ref039","doi-asserted-by":"crossref","first-page":"7474","DOI":"10.1038\/s41598-020-64178-y","article-title":"Identification, characterization and expression analysis of calmodulin and calmodulin-like proteins in Solanum pennellii","volume":"10","author":"J Shi","year":"2020","journal-title":"Sci Rep"},{"key":"pone.0359622.ref040","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/1471-2164-14-216","article-title":"CAMTA 1 regulates drought responses in Arabidopsis thaliana","volume":"14","author":"N Pandey","year":"2013","journal-title":"BMC Genomics"},{"key":"pone.0359622.ref041","doi-asserted-by":"crossref","first-page":"752246","DOI":"10.3389\/fpls.2021.752246","article-title":"TypiCal but DeliCate Ca++re: dissecting the essence of calcium signaling network as a robust response coordinator of versatile abiotic and biotic stimuli in plants","volume":"12","author":"N Patra","year":"2021","journal-title":"Front Plant Sci"},{"issue":"10","key":"pone.0359622.ref042","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0140591","article-title":"Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) improves salinity tolerance in tobacco","volume":"10","author":"Y Xiang","year":"2015","journal-title":"PLoS One"},{"key":"pone.0359622.ref043","doi-asserted-by":"crossref","first-page":"144004","DOI":"10.1016\/j.gene.2019.144004","article-title":"Role of calreticulin in biotic and abiotic stress signalling and tolerance mechanisms in plants","volume":"714","author":"R Joshi","year":"2019","journal-title":"Gene"},{"issue":"1","key":"pone.0359622.ref044","article-title":"Novel anti-oxidative role of calreticulin in protecting A549 human type II alveolar epithelial cells against hypoxic injury","volume":"294","author":"L Jia","year":"2008","journal-title":"Am J Physiol Cell Physiol"},{"issue":"2","key":"pone.0359622.ref045","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.cbi.2013.09.017","article-title":"Calreticulin transacetylase mediated upregulation of thioredoxin by 7,8-diacetoxy-4-methylcoumarin enhances the antioxidant potential and the expression of vascular endothelial growth factor in peripheral blood mononuclear cells","volume":"206","author":"R Joshi","year":"2013","journal-title":"Chem Biol Interact"},{"issue":"3","key":"pone.0359622.ref046","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1104\/pp.107.098103","article-title":"Thioredoxin-linked proteins are reduced during germination of Medicago truncatula seeds","volume":"144","author":"F Alkhalfioui","year":"2007","journal-title":"Plant Physiol"},{"issue":"6","key":"pone.0359622.ref047","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/s10059-012-0080-8","article-title":"Overexpression of Arabidopsis translationally controlled tumor protein gene AtTCTP enhances drought tolerance with rapid ABA-induced stomatal closure","volume":"33","author":"Y-M Kim","year":"2012","journal-title":"Mol Cells"},{"key":"pone.0359622.ref048","doi-asserted-by":"crossref","first-page":"100185","DOI":"10.1016\/j.plgene.2019.100185","article-title":"Homology modeling and molecular dynamics simulations of a cassava translationally controlled tumor protein (MeTCTP)","volume":"19","author":"DN Marques","year":"2019","journal-title":"Plant Gene"},{"key":"pone.0359622.ref049","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/1471-2229-13-135","article-title":"The effect of translationally controlled tumour protein (TCTP) on programmed cell death in plants","volume":"13","author":"MC Hoepflinger","year":"2013","journal-title":"BMC Plant Biol"},{"key":"pone.0359622.ref050","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1186\/s12870-015-0500-y","article-title":"OsTCTP, encoding a translationally controlled tumor protein, plays an important role in mercury tolerance in rice","volume":"15","author":"ZQ Wang","year":"2015","journal-title":"BMC Plant Biol"},{"issue":"4","key":"pone.0359622.ref051","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/s00299-023-02985-z","article-title":"Does translationally controlled tumor protein (TCTP) have the potential to produce crops with increased growth and tolerance to environmental stresses?","volume":"42","author":"DN Marques","year":"2023","journal-title":"Plant Cell Rep"},{"key":"pone.0359622.ref052","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11240-014-0426-3","article-title":"Cotton TCTP1 gene encoding a translationally controlled tumor protein participates in plant response and tolerance to aphids","volume":"117","author":"JM Zhang","year":"2014","journal-title":"Plant Cell Tiss Organ Cult"},{"issue":"13","key":"pone.0359622.ref053","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1002\/pmic.200900835","article-title":"Thioredoxin targets in Arabidopsis roots","volume":"10","author":"CH Marchand","year":"2010","journal-title":"Proteomics"},{"issue":"6","key":"pone.0359622.ref054","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1093\/pcp\/pct037","article-title":"Systematic exploration of thioredoxin target proteins in plant mitochondria","volume":"54","author":"K Yoshida","year":"2013","journal-title":"Plant Cell Physiol"},{"issue":"7","key":"pone.0359622.ref055","doi-asserted-by":"crossref","first-page":"1568","DOI":"10.3390\/ijms20071568","article-title":"Mitochondria are important determinants of the aging of seeds","volume":"20","author":"E Ratajczak","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"6","key":"pone.0359622.ref056","doi-asserted-by":"crossref","first-page":"10868","DOI":"10.3390\/ijms150610868","article-title":"Proteomic analysis of embryogenesis and the acquisition of seed dormancy in Norway maple (Acer platanoides L.)","volume":"15","author":"AM Staszak","year":"2014","journal-title":"Int J Mol Sci"},{"key":"pone.0359622.ref057","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jplph.2016.02.017","article-title":"Analysis of the embryo proteome of sycamore (Acer pseudoplatanus L.) seeds reveals a distinct class of proteins regulating dormancy release","volume":"195","author":"TA Paw\u0142owski","year":"2016","journal-title":"J Plant Physiol"},{"issue":"4","key":"pone.0359622.ref058","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1093\/jxb\/erz508","article-title":"Seed comparative genomics in three coffee species identify desiccation tolerance mechanisms in intermediate seeds","volume":"71","author":"AK Stavrinides","year":"2020","journal-title":"J Exp Bot"},{"issue":"1","key":"pone.0359622.ref059","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s00344-018-9841-8","article-title":"The occurrence of peroxiredoxins and changes in redox state in Acer platanoides and Acer pseudoplatanus during seed development","volume":"38","author":"E Ratajczak","year":"2018","journal-title":"J Plant Growth Regul"},{"issue":"1","key":"pone.0359622.ref060","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/s10725-013-9832-5","article-title":"Factors influencing the storability of Fagus sylvatica L. seeds after release from dormancy","volume":"72","author":"S Pukacka","year":"2013","journal-title":"Plant Growth Regul"},{"issue":"1","key":"pone.0359622.ref061","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s11738-005-0030-6","article-title":"Decrease in beech (Fagus sylvatica) seed viability caused by temperature and humidity conditions as related to membrane damage and lipid composition","volume":"27","author":"E Ratajczak","year":"2005","journal-title":"Acta Physiol Plant"},{"issue":"3","key":"pone.0359622.ref062","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s10725-010-9482-9","article-title":"Desiccation tolerant plants as model systems to study redox regulation of protein thiols","volume":"62","author":"L Colville","year":"2010","journal-title":"Plant Growth Regul"},{"issue":"13","key":"pone.0359622.ref063","doi-asserted-by":"crossref","first-page":"7082","DOI":"10.3390\/ijms23137082","article-title":"Molecular Evolution and Functional Divergence of Stress-Responsive Cu\/Zn Superoxide Dismutases in Plants","volume":"23","author":"G Zhou","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"issue":"4","key":"pone.0359622.ref064","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1104\/pp.109.139204","article-title":"In Vivo Cell Wall Loosening by Hydroxyl Radicals during Cress Seed Germination and Elongation Growth","volume":"150","author":"K Mu\u0308ller","year":"2009","journal-title":"Plant Physiol"}],"container-title":["PLOS One"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0359622","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:53:42Z","timestamp":1791222822000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0359622"}},"subtitle":[],"editor":[{"given":"Ghulam","family":"Jeelani","sequence":"first","affiliation":[{"name":"The University of Tokyo: Tokyo Daigaku","place":["JAPAN"]}],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":64,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,10,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0359622","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]}}}