{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:04:03Z","timestamp":1790877843161,"version":"4.1.0"},"reference-count":62,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Research Office, UAEU","award":["G00004332"],"award-info":[{"award-number":["G00004332"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Excess dietary sugar is a major contributor to metabolic disorders, including type 2 diabetes mellitus (T2DM). This study investigates the physiological, biochemical, behavioral, and molecular effects of high-sucrose diets in\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                      and evaluates the modulatory effects of metformin under these conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Flies were reared on normal and high-sucrose diets (5% and 15%), with or without metformin treatment (5 mM and 15 mM). Pupation, survival, locomotor activity (negative geotaxis), trehalose levels, antioxidant enzyme activities (SOD, GST), and expression of stress-related genes (Mn-SOD, Cu\/Zn-SOD, MTH, CAT, Hsp40, Hsp70) were assessed after 10 days of treatment. Gene expression levels were quantified relative to the normal control group using RpS20 as the housekeeping reference.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>High-sucrose diets significantly reduced pupation and survival rates, impaired locomotor performance, increased trehalose levels, and decreased antioxidant enzyme activities. These diets were also associated with downregulation of stress- and antioxidant-related genes. Metformin treatment partially attenuated several of these alterations relative to the corresponding sucrose-treated groups, including improvements in pupation, survival, locomotor performance, metabolic parameters, antioxidant enzyme activity, and gene expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      High-sucrose diets induced metabolic disturbances in\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                      , including impaired developmental progression and survival, functional locomotor impairment, carbohydrate imbalance, oxidative stress, and suppression of stress-related gene expression. Metformin partially attenuated several of these changes within the corresponding sucrose-treatment groups. These findings support the utility of\n                      <jats:italic>Drosophila melanogaster<\/jats:italic>\n                      as a tractable model for studying diet-induced metabolic dysfunction and evaluating pharmacological interventions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0359638","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:30:52Z","timestamp":1790875852000},"page":"e0359638","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metformin modulates survival, oxidative stress, and gene expression in a high-sucrose Drosophila diabetes model"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4111-3434","authenticated-orcid":true,"given":"Mohamed","family":"Lotfy","sequence":"first","affiliation":[{"name":"United Arab Emirates University","place":["Al Ain, United Arab Emirates"],"department":["Biology Department, College of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatema","family":"Al Sawai","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["Al Ain, United Arab Emirates"],"department":["Biology Department, College of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhoha","family":"Alyammahi","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["Al Ain, United Arab Emirates"],"department":["Biology Department, College of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biduth","family":"Kundu","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["Al Ain, United Arab Emirates"],"department":["Biology Department, College of Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"pone.0359638.ref001","unstructured":"World Health Organization. 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