{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:48:59Z","timestamp":1791571739925,"version":"4.3.4"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["IIRPSG-2025-01-00953"],"award-info":[{"award-number":["IIRPSG-2025-01-00953"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Diabetic retinopathy (DR) remains one of the leading causes of preventable vision loss. However, high-contrast visual acuity, which is routinely used in the clinic, is often preserved during the early stages of the disease, limiting its usefulness in detecting early functional deficits. Colour vision is a sensitive marker for detecting these early retinal changes. This study aims to investigate whether the Colour Assessment and Diagnosis (CAD) test, a quantitative measure of chromatic sensitivity, can serve as a functional biomarker for the early detection of DR and can reflect the severity of the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods and analysis<\/jats:title>\n                    <jats:p>This prospective observational study will be conducted at the L V Prasad Eye Institute, Hyderabad, India. Adults aged 18 years and above will be recruited into three groups: healthy controls, individuals with diabetes without diabetic retinopathy, and individuals with diabetic retinopathy. All participants will undergo a comprehensive ophthalmic examination, colour vision testing, fundus photography, optical coherence tomography (OCT), optical coherence tomography angiography (OCTA), and systemic metabolic investigations, including glycaemic, renal, and lipid profiles. The primary outcome measures are the Red\u2013Green (RG) and Yellow\u2013Blue (YB) Colour thresholds. Secondary outcome measures include retinal structural parameters and vascular parameters, such as retinal thickness, vessel density, and perfusion density. Systemic metabolic parameters, including HbA1c, random blood glucose, renal function, and lipid profile, will be evaluated as independent variables. Statistical analyses will include correlation, multiple regression, and receiver operating characteristic (ROC) curve analyses to evaluate the diagnostic accuracy of the CAD test in the detection of early DR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The current study is expected to provide evidence whether quantitative colour vision assessment can act as a functional marker of early retinal changes in diabetes and diabetic retinopathy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0359653","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:50:56Z","timestamp":1791568256000},"page":"e0359653","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["COlour vision assessment for the Detection of Early diabetic Retinopathy (CODER): A study protocol"],"prefix":"10.1371","volume":"21","author":[{"given":"Vaishaali","family":"Gunalan","sequence":"first","affiliation":[{"name":"Brien Holden Institute of Optometry and Vision Sciences, L V Prasad Eye Institute","place":["Hyderabad, Telangana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vinitha","family":"Mingi","sequence":"additional","affiliation":[{"name":"Brien Holden Institute of Optometry and Vision Sciences, L V Prasad Eye Institute","place":["Hyderabad, Telangana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5779-7645","authenticated-orcid":true,"given":"Brijesh","family":"Takkar","sequence":"additional","affiliation":[{"name":"Anant Bajaj Retina Institute, L V Prasad Eye Institute","place":["Hyderabad, Telangana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Padmaja Kumari","family":"Rani","sequence":"additional","affiliation":[{"name":"Anant Bajaj Retina Institute, L V Prasad Eye Institute","place":["Hyderabad, Telangana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6087-2690","authenticated-orcid":true,"given":"Amithavikram R.","family":"Hathibelagal","sequence":"additional","affiliation":[{"name":"Brien Holden Institute of Optometry and Vision Sciences, L V Prasad Eye Institute","place":["Hyderabad, Telangana, India"]},{"name":"Prof. Brien Holden Eye Research Centre, L V Prasad Eye Institute","place":["Hyderabad, Telangana, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"pone.0359653.ref001","year":"2025"},{"issue":"6","key":"pone.0359653.ref002","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.4103\/ijo.IJO_2206_21","article-title":"Prevalence of diabetic retinopathy in urban and 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