{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:55:13Z","timestamp":1792000513042,"version":"4.4.0"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016804","name":"Natural Science Foundation of Shenzhen Municipality","doi-asserted-by":"publisher","award":["JCYJ20230807154000002"],"award-info":[{"award-number":["JCYJ20230807154000002"]}],"id":[{"id":"10.13039\/100016804","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen Key Medical Discipline Construction Fund","award":["SZXK070"],"award-info":[{"award-number":["SZXK070"]}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzen Municipality","doi-asserted-by":"publisher","award":["SZSM202311032"],"award-info":[{"award-number":["SZSM202311032"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Medical Foundation","award":["B2025582"],"award-info":[{"award-number":["B2025582"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    RNA structural changes caused by mis-splicing or transcription errors may affect antigenic features of cell surface proteins. They are especially noteworthy in the field of blood transfusion where the discrepancy between DNA-based molecular testing and serological blood typing are often encountered in clinical practice and lead to mismatched blood transfusion. In the Kidd (JK) blood group system weak Jk\n                    <jats:sup>a<\/jats:sup>\n                    (Jk(a\u2009+\u2009\n                    <jats:sup>w<\/jats:sup>\n                    )) phenotype is usually missed in routine serological tests and causes delayed hemolytic transfusion reactions. However molecular bases underlying Jk(a\u2009+\u2009\n                    <jats:sup>w<\/jats:sup>\n                    ) phenotype are still not clear although plentiful genomic DNA-based studies have been conducted. In this study, we used PacBio long-read sequencing to characterize open reading frame (ORF) region of\n                    <jats:italic>SLC14A1<\/jats:italic>\n                    mRNA obtained from reticulocytes of 18 blood donors possessing\n                    <jats:italic>JK*A<\/jats:italic>\n                    alleles. Among them, 8 donors were determined as normal Jk\n                    <jats:sup>a<\/jats:sup>\n                    antigen presentation and 10 donors showed Jk(a\u2009+\u2009\n                    <jats:sup>w<\/jats:sup>\n                    ) phenotype in routine serological tests. We found that Jk(a\u2009+\u2009\n                    <jats:sup>w<\/jats:sup>\n                    ) subjects demonstrated different exon combination patterns compared to those individuals presenting normal Jk\n                    <jats:sup>a<\/jats:sup>\n                    antigens, implying the resulted SLC14A1 protein isoforms could make different contributions to the formation of the JK antigenicity. Moreover, exon skipping and exon inclusion that were frequently found in Jk(a\u2009+\u2009\n                    <jats:sup>w<\/jats:sup>\n                    ) individuals could induce frameshift mutations. In addition, high levels of nucleotide insertions or deletions (indels) at\n                    <jats:italic>130A<\/jats:italic>\n                    position were detected in the transcripts of Jk(a\u2009+\u2009\n                    <jats:sup>w<\/jats:sup>\n                    ) individuals. Technical artifacts were examined using synthesized RNA templates and the results suggested that artifacts generated in reverse transcription and PCR amplification (RT-PCR) processes were unlikely the causes of these observations. All the results indicate that\n                    <jats:italic>SLC14A1<\/jats:italic>\n                    RNA splicing and transcription fidelity play crucial roles in determining Jk\n                    <jats:sup>a<\/jats:sup>\n                    antigen presentation.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0360437","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:30:42Z","timestamp":1791999042000},"page":"e0360437","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alterations of SLC14A1 mRNA structure are frequently detected in weak Jka presenting donors"],"prefix":"10.1371","volume":"21","author":[{"given":"Shuang","family":"Liang","sequence":"first","affiliation":[{"name":"Institute of Transfusion Medicine, Shenzhen Blood Center","place":["Shenzhen, Guangdong, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanwen","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Guangdong Medical University","place":["Zhanjiang, Guangdong, People\u2019s Republic of China"],"department":["Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, Shenzhen Blood Center","place":["Shenzhen, Guangdong, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, Shenzhen Blood Center","place":["Shenzhen, Guangdong, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Transfusion Medicine, Shenzhen Blood Center","place":["Shenzhen, Guangdong, People\u2019s Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Luo","sequence":"additional","affiliation":[{"name":"The Chinese University of Hong Kong","place":["Hong Kong, People\u2019s Republic of China"],"department":["School of Biomedical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Hangzhou Normal University","place":["Hangzhou, Zhejiang, People\u2019s Republic of China"],"department":["School of Pharmacy"]},{"name":"Zhuhai College of Science and Technology","place":["Zhuhai, Guangdong, People\u2019s Republic of China"],"department":["School of Computer Science"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Guangdong Medical University","place":["Zhanjiang, Guangdong, People\u2019s Republic of China"],"department":["Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Feng","sequence":"additional","affiliation":[{"name":"The Chinese University of Hong Kong","place":["Hong Kong, People\u2019s Republic of China"],"department":["School of Biomedical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0142-2603","authenticated-orcid":true,"given":"Jianwei","family":"Ren","sequence":"additional","affiliation":[{"name":"Hong Kong Institute of Science & Innovation, Chinese Academy of Sciences","place":["Hong Kong, People\u2019s Republic of China"],"department":["Centre for 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