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The ELISA kits were used to detect IL-1\u03b2 and IL-18 levels. The immunohistochemical (IHC) staining was used in conjunction with qRT-PCR and Western blot to determine IL-1\u03b2, IL-18, NLRP3, pro Caspase-1, cleaved Caspase-1, GSDMD-FL, and GSDMD-N expression levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In myocardial tissue, the \u03b2-blocker combined with the 5-HT1AR agonist effectively reduced infarction size, mitigated pathological injury, alleviated fibrosis, and prevented apoptosis. Meanwhile, the \u03b2-blocker combined with the 5-HT1AR agonist downregulated IL-1\u03b2 and IL-18 levels in serum and myocardial tissue. Notably, although it barely altered the expression of the pyroptosis precursor proteins pro Caspase\u20111 and GSDMD\u2011FL, the combined therapy strongly suppressed the mRNA and protein levels of NLRP3, cleaved Caspase\u20111, and GSDMD\u2011N, and reduced the ratios of pro Caspase\u20111\/cleaved Caspase\u20111 and GSDMD-FL\/GSDMD-N in myocardial tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\u03b2-blocker combined with the 5-HT1AR agonist relieves MI injuries by suppressing the NLRP3\/Caspase-1\/GSDMD pathway.<\/jats:p>\n                  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