{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:53:50Z","timestamp":1792788830780,"version":"4.5.0"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Guangxi Zhuang Autonomous Region Health Commission Self-Funded Research Project","award":["Z20210988"],"award-info":[{"award-number":["Z20210988"]}],"id":[{"id":"https:\/\/ror.org\/01x9sys47","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003454","name":"Guangxi Zhuang Autonomous Region Health Department","doi-asserted-by":"publisher","award":["S2021034"],"award-info":[{"award-number":["S2021034"]}],"id":[{"id":"10.13039\/501100003454","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>Cervical intraepithelial neoplasia (CIN) is a precursor to cervical cancer, and early detection of residual or recurrent disease is therefore critical for effective management. This study evaluated whether HPV E6\/E7 mRNA status predicts recurrence in patients with CIN who underwent loop electrosurgical excision procedure (LEEP). People\u2019s Hospital of Nanning between January 2019 and July 2022 were included. Patients who underwent LEEP for high-grade cervical lesions at the First Follow-up commenced 4\u20136 months after LEEP and continued for 24 months; abnormal findings during this period prompted referral for colposcopy or cervical biopsy. Of the 91 patients, 3 (3.30%) experienced recurrence within 24 months. Recurrence rates were similar between patients with preoperative E6\/E7 mRNA positivity (3.23%) and negativity (3.45%) (p\u2009=\u20090.956), and between postoperative E6\/E7 mRNA status groups (5.26% in the negative group vs. 0% in the positive group; p\u2009=\u20090.814). Although 66.7% of patients shifted from positive to negative E6\/E7 mRNA status after LEEP (p\u2009&lt;\u20090.001), this change was not associated with recurrence risk. Preoperative E6\/E7 mRNA yielded a sensitivity of 66.67% and specificity of 31.82%, whereas postoperative E6\/E7 mRNA showed a sensitivity of 0% and specificity of 97.30%. In conclusion, E6\/E7 mRNA status before and after LEEP did not predict recurrence in patients with CIN. Despite a significant reduction in E6\/E7 mRNA positivity after surgery, its low sensitivity underscores the need for more effective biomarkers in follow-up care.<\/jats:p>","DOI":"10.1371\/journal.pone.0360914","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:28:48Z","timestamp":1792171728000},"page":"e0360914","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of HPV E6\/E7 mRNA in predicting recurrence after Leep in cervical intraepithelial neoplasia: A study of negative findings"],"prefix":"10.1371","volume":"21","author":[{"given":"Yingmei","family":"Huang","sequence":"first","affiliation":[{"name":"The First People\u2019s Hospital of Nanning","place":["Nanning, Guangxi Zhuang Autonomous Region, China"],"department":["Gynecology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyong","family":"Huang","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Nanning","place":["Nanning, Guangxi Zhuang Autonomous Region, China"],"department":["Gynecology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongmian","family":"Jiang","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Nanning","place":["Nanning, Guangxi Zhuang Autonomous Region, China"],"department":["Pathology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Wei","sequence":"additional","affiliation":[{"name":"The First People\u2019s Hospital of Nanning","place":["Nanning, Guangxi Zhuang Autonomous Region, China"],"department":["Gynecology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Lin","sequence":"additional","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region","place":["Nanning, Guangxi Zhuang Autonomous Region, China"],"department":["Gynecology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6606-366X","authenticated-orcid":true,"given":"Baoli","family":"Xie","sequence":"additional","affiliation":[{"name":"The Reproductive Hospital of Guangxi Zhuang Autonomous Region","place":["Nanning, Guangxi Zhuang Autonomous Region, China"],"department":["Gynecology Department"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"issue":"1","key":"pone.0360914.ref001","doi-asserted-by":"crossref","first-page":"222","DOI":"10.3390\/ijms21010222","article-title":"The role of the cervicovaginal microbiome on the genesis and as a biomarker of premalignant cervical intraepithelial neoplasia and invasive cervical cancer","volume":"21","author":"G Curty","year":"2019","journal-title":"Int J Mol Sci"},{"issue":"3","key":"pone.0360914.ref002","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/1471-0528.16395","article-title":"The use of host cell DNA methylation analysis in the detection and management of women with advanced cervical intraepithelial neoplasia: A review","volume":"128","author":"WW Kremer","year":"2021","journal-title":"BJOG"},{"key":"pone.0360914.ref003","unstructured":"Mitra A, Tzafetas M, Lyons D, Fotopoulou C, Paraskevaidis E, Paraskevaidis E, et al. Cervical intraepithelial neoplasia: Screening and management. Gynecol Oncol. 0(0):0."},{"issue":"6","key":"pone.0360914.ref004","first-page":"663","article-title":"Loop electrosurgical excision procedure vs. cold knife cone in treatment of cervical intraepithelial neoplasia: review of 447 cases","volume":"47","author":"XI Wang","year":"2017","journal-title":"Ann Clin Lab Sci"},{"issue":"1","key":"pone.0360914.ref005","first-page":"1","article-title":"Cervical colposcopy: Indications and risk assessment","volume":"1","author":"JV Burness","year":"2023","journal-title":"Cervical Colposcopy"},{"key":"pone.0360914.ref006","article-title":"Impact of loop electrosurgical excision procedure for cervical intraepithelial neoplasia on HIV-1 genital shedding: a prospective cohort study","author":"MJ Huchko","year":"2023","journal-title":"BMC Infect Dis"},{"issue":"3","key":"pone.0360914.ref007","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.ijgo.2015.07.026","article-title":"Systematic reviews and meta-analyses of benefits and harms of cryotherapy, LEEP, and cold knife conization to treat cervical intraepithelial neoplasia","volume":"132","author":"N Santesso","year":"2016","journal-title":"Int J Gynaecol Obstet"},{"key":"pone.0360914.ref008","unstructured":"Dou Y, Zhang X, Li Y, Wang F, Xie X, Wang X. Triage for management of cervical high-grade squamous intraepithelial lesion patients with positive margin by conization: a retrospective analysis. N\/A. 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