{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:53:40Z","timestamp":1792173220527,"version":"4.4.0"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A-182478\/1"],"award-info":[{"award-number":["31003A-182478\/1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A-163347"],"award-info":[{"award-number":["31003A-163347"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["320030-231787"],"award-info":[{"award-number":["320030-231787"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["PZ00P3_223607 \/ 1"],"award-info":[{"award-number":["PZ00P3_223607 \/ 1"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS One"],"abstract":"<jats:p>\n                    In humans, the membrane-bound serine protease prostasin encoded by\n                    <jats:italic>Prss8<\/jats:italic>\n                    is associated with preeclampsia, a gestational hypertension disorder affecting blood supply of the placenta. Deficiency of\n                    <jats:italic>Prss8<\/jats:italic>\n                    in mice resulted in embryonic lethality by embryonic day (E) 14.5 and impaired maturation of placental labyrinth. We found in\n                    <jats:italic>Prss8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>-\/-<\/jats:italic>\n                    <\/jats:sup>\n                    embryos at E11.5 and E12.5 a reduced frequency of both circulating primitive and erythro-myeloid progenitor (EMP)-derived definitive erythroblasts in the yolk sac, placenta and fetal liver. Single-cell RNA sequencing analyses revealed a downregulation of fetal hemoglobin genes and an upregulation of ribosomal genes in primitive erythroid cells of\n                    <jats:italic>Prss8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>-\/-<\/jats:italic>\n                    <\/jats:sup>\n                    embryos, indicating a defective erythroid maturation. We suggest that prostasin regulates primitive erythropoiesis in a cell-extrinsic manner, since its expression was absent in circulating erythroid cells. Indeed, the yolk sac vascular remodeling of\n                    <jats:italic>Prss8<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>-\/-<\/jats:italic>\n                    <\/jats:sup>\n                    embryos was impaired as evidenced by reduced vessel branching and junctions, likely affecting placenta vascularization and contributing to embryonic demise. Our findings unveil a novel role for prostasin during developmental erythropoiesis and vascular patterning and provide insight into how\n                    <jats:italic>Prss8<\/jats:italic>\n                    dysregulation may contribute to placental pathologies such as preeclampsia.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0360958","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:29:28Z","timestamp":1792171768000},"page":"e0360958","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impaired erythroid maturation in murine embryos upon loss of the preeclampsia-associated serine protease prostasin Prostasin loss impairs erythropoiesis"],"prefix":"10.1371","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2148-5372","authenticated-orcid":true,"given":"Sara","family":"Di Carlo","sequence":"first","affiliation":[{"name":"University of Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Biomedical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9847-5562","authenticated-orcid":true,"given":"Adrian","family":"Salas-Bastos","sequence":"additional","affiliation":[{"name":"Institute of Anatomy, Stem Cell Biology, University of Zurich","place":["Zurich, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariela","family":"Castelblanco Castelblanco","sequence":"additional","affiliation":[{"name":"University of Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Biomedical Sciences"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muriel","family":"Auberson","sequence":"additional","affiliation":[{"name":"University of Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Biomedical 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Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Biomedical Sciences"]},{"name":"Hematology Service, Departments of Clinical Oncology and Laboratory Medicine and Pathology, Lausanne University Hospital (CHUV) and University of Lausanne","place":["Lausanne, Switzerland"]},{"name":"University of Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Fundamental Oncology"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3434-0022","authenticated-orcid":true,"given":"Olaia","family":"Naveiras","sequence":"additional","affiliation":[{"name":"University of Lausanne","place":["Lausanne, Switzerland"],"department":["Department of Biomedical Sciences"]},{"name":"Hematology Service, Departments of Clinical Oncology and Laboratory Medicine and Pathology, Lausanne University Hospital (CHUV) and University of Lausanne","place":["Lausanne, Switzerland"]},{"name":"University of Lausanne","place":["Lausanne, 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