{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:08:50Z","timestamp":1792710530547,"version":"4.5.0"},"reference-count":37,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2007,7,13]],"date-time":"2007-07-13T00:00:00Z","timestamp":1184284800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.0030097","type":"journal-article","created":{"date-parts":[[2007,7,9]],"date-time":"2007-07-09T18:00:15Z","timestamp":1184004015000},"page":"e97","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":206,"title":["The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence"],"prefix":"10.1371","volume":"3","author":[{"given":"Paula","family":"Ristow","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Bourhy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via Weykamp da Cruz","family":"McBride","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio Pereira","family":"Figueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Huerre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Ave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle Saint","family":"Girons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert I","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2007,7,13]]},"reference":[{"key":"ppat-0030097-b001","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1097\/01.qco.0000178824.05715.2c","article-title":"Leptospirosis.","volume":"18","year":"2005","journal-title":"Curr Opin Infect Dis"},{"key":"ppat-0030097-b002","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1016\/S1473-3099(03)00830-2","article-title":"Leptospirosis: A zoonotic disease of global importance.","volume":"3","year":"2003","journal-title":"Lancet Infect Dis"},{"key":"ppat-0030097-b003","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1128\/CMR.14.2.296-326.2001","article-title":"Leptospirosis.","volume":"14","year":"2001","journal-title":"Clin Microbiol Rev"},{"key":"ppat-0030097-b004","first-page":"237","article-title":"Leptospirosis worldwide, 1999.","volume":"74","author":"WHO","year":"1999","journal-title":"Weekly Epidemiol Rec"},{"key":"ppat-0030097-b005","doi-asserted-by":"crossref","first-page":"6356","DOI":"10.1128\/IAI.00460-06","article-title":"A newly identified leptospiral adhesin mediates attachment to laminin.","volume":"74","year":"2006","journal-title":"Infect Immun"},{"key":"ppat-0030097-b006","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1574-6968.2000.tb09034.x","article-title":"Identification of a 36-kDa fibronectin-binding protein expressed by a virulent variant of Leptospira interrogans serovar icterohaemorrhagiae.","volume":"185","year":"2000","journal-title":"FEMS Microbiol Lett"},{"key":"ppat-0030097-b007","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1128\/IAI.70.1.315-322.2002","article-title":"Cytotoxic activities of Leptospira interrogans hemolysin SphH as a pore-forming protein on mammalian cells.","volume":"70","year":"2002","journal-title":"Infect Immun"},{"key":"ppat-0030097-b008","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.femsre.2003.10.004","article-title":"Outer membrane proteins of pathogenic spirochetes.","volume":"28","year":"2004","journal-title":"FEMS Microbiol Rev"},{"key":"ppat-0030097-b009","doi-asserted-by":"crossref","first-page":"2164","DOI":"10.1128\/JB.186.7.2164-2172.2004","article-title":"Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis.","volume":"186","year":"2004","journal-title":"J Bacteriol"},{"key":"ppat-0030097-b010","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1038\/nature01597","article-title":"Unique and physiological and pathogenic features of Leptospira interrogans revealed by whole genome sequencing.","volume":"422","year":"2003","journal-title":"Nature"},{"key":"ppat-0030097-b011","doi-asserted-by":"crossref","first-page":"14560","DOI":"10.1073\/pnas.0603979103","article-title":"Genome reduction in Leptospira borgpetersenii reflects limited transmission potential.","volume":"103","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ppat-0030097-b012","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1093\/cid\/10.Supplement_2.S274","article-title":"Molecular Koch's postulates applied to microbial pathogenicity.","volume":"10","year":"1988","journal-title":"Rev Infect Dis"},{"key":"ppat-0030097-b013","doi-asserted-by":"crossref","first-page":"3255","DOI":"10.1128\/JB.187.9.3255-3258.2005","article-title":"Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.","volume":"187","year":"2005","journal-title":"J Bacteriol"},{"key":"ppat-0030097-b014","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/S0378-1097(03)00619-0","article-title":"Molecular cloning and characterization of a novel leptospiral lipoprotein with OmpA domain.","volume":"226","year":"2003","journal-title":"FEMS Microbiol Lett"},{"key":"ppat-0030097-b015","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.femsle.2005.02.004","article-title":"Whole-genome analysis of Leptospira interrogans to identify potential vaccine candidates against leptospirosis.","volume":"244","year":"2005","journal-title":"FEMS Microbiol Lett"},{"key":"ppat-0030097-b016","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1186\/1471-2164-7-293","article-title":"In silico and microarray-based genomic approaches to identifying potential vaccine candidates against Leptospira interrogans.","volume":"7","year":"2006","journal-title":"BMC Genomics"},{"key":"ppat-0030097-b017","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1128\/IAI.00741-06","article-title":"Characterization of the outer membrane proteome of Leptospira interrogans expressed during acute lethal infection.","volume":"75","year":"2007","journal-title":"Infect Immun"},{"key":"ppat-0030097-b018","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1099\/mic.0.28317-0","article-title":"Lipoprotein computational prediction in spirochaetal genomes.","volume":"152","year":"2006","journal-title":"Microbiology"},{"key":"ppat-0030097-b019","doi-asserted-by":"crossref","first-page":"1652","DOI":"10.1110\/ps.0303703","article-title":"Prediction of lipoprotein signal peptides in Gram-negative bacteria.","volume":"12","year":"2003","journal-title":"Protein Sci"},{"key":"ppat-0030097-b020","doi-asserted-by":"crossref","first-page":"4936","DOI":"10.1128\/IAI.70.9.4936-4945.2002","article-title":"Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins.","volume":"70","year":"2002","journal-title":"Infect Immun"},{"key":"ppat-0030097-b021","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1128\/IAI.59.3.1131-1140.1991","article-title":"Changes in the surface of Leptospira interrogans serovar grippotyphosa during in vitro cultivation.","volume":"59","year":"1991","journal-title":"Infect Immun"},{"key":"ppat-0030097-b022","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1111\/j.1365-2958.2003.03903.x","article-title":"Structure of the OmpA-like domain of RmpM from Neisseria meningitidis.","volume":"51","year":"2004","journal-title":"Mol Microbiol"},{"key":"ppat-0030097-b023","first-page":"333","article-title":"Proposal for a peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins.","volume":"12","year":"1995","journal-title":"Mol Microbiol"},{"key":"ppat-0030097-b024","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1128\/IAI.63.1.38-42.1995","article-title":"Epitope mapping of the Pseudomonas aeruginosa major outer membrane porin protein OprF.","volume":"63","year":"1995","journal-title":"Infect Immun"},{"key":"ppat-0030097-b025","doi-asserted-by":"crossref","first-page":"3937","DOI":"10.1128\/IAI.71.7.3937-3946.2003","article-title":"The C-terminal domain of Salmonella enterica serovar typhimurium OmpA is an immunodominant antigen in mice but appears to be only partially exposed on the bacterial cell surface.","volume":"71","year":"2003","journal-title":"Infect Immun"},{"key":"ppat-0030097-b026","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1038\/2983","article-title":"Structure of the outer membrane protein A transmembrane domain.","volume":"5","year":"1998","journal-title":"Nat Struct Biol"},{"key":"ppat-0030097-b027","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0882-4010(03)00098-6","article-title":"Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: Evidence of its role in P. multocida interaction with extracellular matrix molecules.","volume":"35","year":"2003","journal-title":"Microb Pathog"},{"key":"ppat-0030097-b028","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1016\/j.bbrc.2005.03.097","article-title":"Escherichia coli outer membrane protein A adheres to human brain microvascular endothelial cells.","volume":"330","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"ppat-0030097-b029","doi-asserted-by":"crossref","first-page":"8008","DOI":"10.1128\/AEM.71.12.8008-8015.2005","article-title":"Differential binding of Escherichia coli O157:H7 to alfalfa, human epithelial cells, and plastic is mediated by a variety of surface structures.","volume":"71","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"ppat-0030097-b030","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1128\/IAI.74.5.2676-2685.2006","article-title":"Outer membrane protein A of Escherichia coli O157:H7 stimulates dendritic cell activation.","volume":"74","year":"2006","journal-title":"Infect Immun"},{"key":"ppat-0030097-b031","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.immuni.2005.03.008","article-title":"Complexity and complementarity of outer membrane protein A recognition by cellular and humoral innate immunity receptors.","volume":"22","year":"2005","journal-title":"Immunity"},{"key":"ppat-0030097-b032","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1128\/JB.94.1.27-31.1967","article-title":"Differentiation of pathogenic and saprophytic leptospires.","volume":"94","year":"1967","journal-title":"J Bacteriol"},{"key":"ppat-0030097-b033","first-page":"45","article-title":"Nutrition of Leptospira pomona and growth of 13 other serotypes: Fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80.","volume":"26","year":"1965","journal-title":"Am J Vet Res"},{"key":"ppat-0030097-b034","doi-asserted-by":"crossref","unstructured":"LouvelHPicardeauM\n\t\t\t\t\t2007\n\t\t\t\t\tGenetic manipulation of Leptospira biflexa.\n\t\t\t\t\tIn:\n\t\t\t\t\tCoicoRKowalikTFQuarlesJMStevensonBTaylorR\n\t\t\t\t\tCurrent protocols in microbiology\n\t\t\t\t\tHoboken (New Jersey)\n\t\t\t\t\tJ. Wiley and Sons","DOI":"10.1002\/9780471729259.mc12e04s05"},{"key":"ppat-0030097-b035","doi-asserted-by":"crossref","first-page":"4853","DOI":"10.1128\/IAI.73.8.4853-4863.2005","article-title":"Surfaceome of Leptospira spp.","volume":"73","year":"2005","journal-title":"Infect Immun"},{"key":"ppat-0030097-b036","unstructured":"BancroftJDCookHCTurnerDR\n\t\t\t\t\t1994\n\t\t\t\t\tManual of histological techniques and their diagnostic application\n\t\t\t\t\tOxford\n\t\t\t\t\tChurchill Livingstone\n\t\t\t\t\t457"},{"key":"ppat-0030097-b037","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1128\/IAI.68.4.2276-2285.2000","article-title":"The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection.","volume":"68","year":"2000","journal-title":"Infect Immun"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.0030097","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T18:41:04Z","timestamp":1588876864000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.0030097"}},"subtitle":[],"editor":[{"given":"Michael R","family":"Wessells","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2007,7,13]]},"references-count":37,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2007,7,13]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.0030097","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,13]]}}}