{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:41:48Z","timestamp":1790214108045,"version":"4.1.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2008,10,31]],"date-time":"2008-10-31T00:00:00Z","timestamp":1225411200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1000191","type":"journal-article","created":{"date-parts":[[2008,10,30]],"date-time":"2008-10-30T22:36:00Z","timestamp":1225406160000},"page":"e1000191","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":49,"title":["Repetitive N-WASP\u2013Binding Elements of the Enterohemorrhagic Escherichia coli Effector EspFU Synergistically Activate Actin Assembly"],"prefix":"10.1371","volume":"4","author":[{"given":"Kenneth G.","family":"Campellone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Chun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Robbins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anosha D.","family":"Siripala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma J.","family":"McGhie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Hayward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Welch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Rosen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vassilis","family":"Koronakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Leong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2008,10,31]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1172\/JCI12404","article-title":"Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic Escherichia coli.","volume":"107","author":"MS Donnenberg","year":"2001","journal-title":"J Clin Invest"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli.","volume":"2","author":"JB Kaper","year":"2004","journal-title":"Nat Rev Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1574-6968.2006.00119.x","article-title":"A comparison of enteropathogenic and enterohaemorrhagic Escherichia coli pathogenesis.","volume":"255","author":"KJ Spears","year":"2006","journal-title":"FEMS Microbiol Lett"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"3597","DOI":"10.1073\/pnas.0400326101","article-title":"Dissecting virulence: systematic and functional analyses of a pathogenicity island.","volume":"101","author":"W Deng","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"2171","DOI":"10.1128\/IAI.68.4.2171-2182.2000","article-title":"Role of tir and intimin in the virulence of rabbit enteropathogenic Escherichia coli serotype O103:H2.","volume":"68","author":"O Marches","year":"2000","journal-title":"Infect Immun"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"7129","DOI":"10.1128\/IAI.71.12.7129-7139.2003","article-title":"Critical roles for stx2, eae, and tir in enterohemorrhagic Escherichia coli-induced diarrhea and intestinal inflammation in infant rabbits.","volume":"71","author":"JM Ritchie","year":"2003","journal-title":"Infect Immun"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"3689","DOI":"10.1128\/IAI.68.6.3689-3695.2000","article-title":"Role of EspB in experimental human enteropathogenic Escherichia coli infection.","volume":"68","author":"CO Tacket","year":"2000","journal-title":"Infect Immun"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1111\/j.1462-5822.2007.01087.x","article-title":"EspF(U), a type III-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by <italic>E. coli<\/italic> O157:H7.","volume":"10","author":"JM Ritchie","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"14941","DOI":"10.1073\/pnas.0604891103","article-title":"An extensive repertoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination.","volume":"103","author":"T Tobe","year":"2006","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.mib.2004.12.010","article-title":"EPEC&apos;s weapons of mass subversion.","volume":"8","author":"P Dean","year":"2005","journal-title":"Curr Opin Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1128\/IAI.73.5.2573-2585.2005","article-title":"Enteropathogenic and enterohemorrhagic Escherichia coli infections: translocation, translocation, translocation.","volume":"73","author":"J Garmendia","year":"2005","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1046\/j.1365-2958.2003.03830.x","article-title":"Self-association of EPEC intimin mediated by the beta-barrel-containing anchor domain: a role in clustering of the Tir receptor.","volume":"51","author":"T Touze","year":"2004","journal-title":"Mol Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1038\/14087","article-title":"Enteropathogenic E. coli acts through WASP and Arp2\/3 complex to form actin pedestals.","volume":"1","author":"D Kalman","year":"1999","journal-title":"Nat Cell Biol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1462-5822.2004.00364.x","article-title":"Enterohaemorrhagic and enteropathogenic Escherichia coli use different mechanisms for actin pedestal formation that converge on N-WASP.","volume":"6","author":"S Lommel","year":"2004","journal-title":"Cell Microbiol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1093\/embo-reports\/kve197","article-title":"Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells.","volume":"2","author":"S Lommel","year":"2001","journal-title":"EMBO Rep"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/nrm2026","article-title":"The ARP2\/3 complex: an actin nucleator comes of age.","volume":"7","author":"ED Goley","year":"2006","journal-title":"Nat Rev Mol Cell Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.ceb.2005.12.003","article-title":"Protein complexes regulating Arp2\/3-mediated actin assembly.","volume":"18","author":"TE Stradal","year":"2006","journal-title":"Curr Opin Cell Biol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0092-8674(00)80732-1","article-title":"The interaction between N-WASP and the Arp2\/3 complex links Cdc42-dependent signals to actin assembly.","volume":"97","author":"R Rohatgi","year":"1999","journal-title":"Cell"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"26448","DOI":"10.1074\/jbc.M103856200","article-title":"Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-Arp2\/3 pathway.","volume":"276","author":"R Rohatgi","year":"2001","journal-title":"J Biol Chem"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/35074551","article-title":"WIP regulates N-WASP-mediated actin polymerization and filopodium formation.","volume":"3","author":"N Martinez-Quiles","year":"2001","journal-title":"Nat Cell Biol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.cell.2004.06.027","article-title":"Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex.","volume":"118","author":"HY Ho","year":"2004","journal-title":"Cell"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1111\/j.1365-2958.2007.05626.x","article-title":"Enteropathogenic Escherichia coli Tir is an SH2\/3 ligand that recruits and activates tyrosine kinases required for pedestal formation.","volume":"63","author":"B Bommarius","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/ncb1148","article-title":"Phosphorylation of the enteropathogenic <italic>E. coli<\/italic> receptor by the Src-family kinase c-Fyn triggers actin pedestal formation.","volume":"6","author":"N Phillips","year":"2004","journal-title":"Nat Cell Biol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"3520","DOI":"10.1091\/mbc.E04-02-0093","article-title":"Enteropathogenic Escherichia coli use redundant tyrosine kinases to form actin pedestals.","volume":"15","author":"A Swimm","year":"2004","journal-title":"Mol Biol Cell"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1083\/jcb.200306032","article-title":"Clustering of Nck by a 12-residue Tir phosphopeptide is sufficient to trigger localized actin assembly.","volume":"164","author":"KG Campellone","year":"2004","journal-title":"J Cell Biol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1111\/j.1462-5822.2007.01103.x","article-title":"Attaching effacing Escherichia coli and paradigms of Tir-triggered actin polymerization: getting off the pedestal.","volume":"10","author":"G Frankel","year":"2008","journal-title":"Cell Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/nrmicro1391","article-title":"Exploiting pathogenic Escherichia coli to model transmembrane receptor signalling.","volume":"4","author":"RD Hayward","year":"2006","journal-title":"Nat Rev Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.devcel.2004.07.004","article-title":"EspF(U) is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly.","volume":"7","author":"KG Campellone","year":"2004","journal-title":"Dev Cell"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1111\/j.1462-5822.2004.00459.x","article-title":"TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton.","volume":"6","author":"J Garmendia","year":"2004","journal-title":"Cell Microbiol"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"2242","DOI":"10.1111\/j.1462-5822.2007.00954.x","article-title":"Enterohaemorrhagic and enteropathogenic Escherichia coli Tir proteins trigger a common Nck-independent actin assembly pathway.","volume":"9","author":"MJ Brady","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1111\/j.1462-5822.2006.00728.x","article-title":"Enterohaemorrhagic Escherichia coli Tir requires a C-terminal 12-residue peptide to initiate EspF(U)-mediated actin assembly and harbours N-terminal sequences that influence pedestal length.","volume":"8","author":"KG Campellone","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1111\/j.1462-5822.2006.00723.x","article-title":"Characterization of TccP-mediated N-WASP activation during enterohaemorrhagic Escherichia coli infection.","volume":"8","author":"J Garmendia","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1083\/jcb.200705021","article-title":"The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways.","volume":"178","author":"NM Alto","year":"2007","journal-title":"J Cell Biol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cell.2005.10.031","article-title":"Identification of a bacterial type III effector family with G protein mimicry functions.","volume":"124","author":"NM Alto","year":"2006","journal-title":"Cell"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"5715","DOI":"10.1128\/JCM.43.11.5715-5720.2005","article-title":"Distribution of tccP in clinical enterohemorrhagic and enteropathogenic Escherichia coli isolates.","volume":"43","author":"J Garmendia","year":"2005","journal-title":"J Clin Microbiol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1111\/j.1462-5822.2007.00913.x","article-title":"Tyrosine phosphorylation controls cortactin binding to two enterohaemorrhagic Escherichia coli effectors: Tir and EspFu\/TccP.","volume":"9","author":"VV Cantarelli","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.biophys.35.040405.101936","article-title":"Regulation of actin filament assembly by Arp2\/3 complex and formins.","volume":"36","author":"TD Pollard","year":"2007","journal-title":"Annu Rev Biophys Biomol Struct"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"11306","DOI":"10.1073\/pnas.211420498","article-title":"CR16 forms a complex with N-WASP in brain and is a novel member of a conserved proline-rich actin-binding protein family.","volume":"98","author":"HY Ho","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.molcel.2004.09.018","article-title":"Critical conformational changes in the Arp2\/3 complex are induced by nucleotide and nucleation promoting factor.","volume":"16","author":"ED Goley","year":"2004","journal-title":"Mol Cell"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1038\/35004513","article-title":"Autoinhibition and activation mechanisms of the Wiskott-Aldrich syndrome protein.","volume":"404","author":"AS Kim","year":"2000","journal-title":"Nature"},{"key":"ref41","article-title":"Structural mechanism of WASP activation by the enterohaemorrhagic <italic>E. coli<\/italic> effector EspF(U).","author":"HC Cheng","year":"2008","journal-title":"Nature"},{"key":"ref42","article-title":"The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency.","author":"NA Sallee","year":"2008","journal-title":"Nature"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.1093\/emboj\/17.10.2767","article-title":"Neural Wiskott-Aldrich syndrome protein is implicated in the actin-based motility of Shigella flexneri.","volume":"17","author":"T Suzuki","year":"1998","journal-title":"EMBO J"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1468","DOI":"10.1111\/j.1365-2958.2007.05602.x","article-title":"Increased adherence and actin pedestal formation by dam-deficient enterohaemorrhagic Escherichia coli O157:H7.","volume":"63","author":"KG Campellone","year":"2007","journal-title":"Mol Microbiol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1046\/j.1365-2958.2002.02817.x","article-title":"A tyrosine-phosphorylated 12-amino-acid sequence of enteropathogenic Escherichia coli Tir binds the host adaptor protein Nck and is required for Nck localization to actin pedestals.","volume":"43","author":"KG Campellone","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1111\/j.1365-2958.2005.04558.x","article-title":"Nck-independent actin assembly is mediated by two phosphorylated tyrosines within enteropathogenic Escherichia coli Tir.","volume":"56","author":"KG Campellone","year":"2005","journal-title":"Mol Microbiol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1038\/ncb1001-897","article-title":"N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility.","volume":"3","author":"SB Snapper","year":"2001","journal-title":"Nat Cell Biol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.cell.2008.05.032","article-title":"WHAMM is an Arp2\/3 complex activator that binds microtubules and functions in ER to Golgi transport.","volume":"134","author":"KG Campellone","year":"2008","journal-title":"Cell"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1093\/emboj\/20.9.2131","article-title":"Cooperation between actin-binding proteins of invasive Salmonella: SipA potentiates SipC nucleation and bundling of actin.","volume":"20","author":"EJ McGhie","year":"2001","journal-title":"EMBO J"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1000191","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,10,20]],"date-time":"2018-10-20T15:03:15Z","timestamp":1540047795000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1000191"}},"subtitle":[],"editor":[{"given":"Jorge E.","family":"Gal\u00e1n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2008,10,31]]},"references-count":49,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2008,10,31]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1000191","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,31]]}}}