{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:29:53Z","timestamp":1792740593155,"version":"4.5.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2008,11,28]],"date-time":"2008-11-28T00:00:00Z","timestamp":1227830400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1000226","type":"journal-article","created":{"date-parts":[[2008,11,27]],"date-time":"2008-11-27T22:46:29Z","timestamp":1227825989000},"page":"e1000226","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":156,"title":["A Structural Model of the Staphylococcus aureus ClfA\u2013Fibrinogen Interaction Opens New Avenues for the Design of Anti-Staphylococcal Therapeutics"],"prefix":"10.1371","volume":"4","author":[{"given":"Vannakambadi K.","family":"Ganesh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose J.","family":"Rivera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuel","family":"Smeds","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ping","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Gabriela","family":"Bowden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth R.","family":"Wann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivasankarappa","family":"Gurusiddappa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Ross","family":"Fitzgerald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"H\u00f6\u00f6k","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2008,11,28]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1056\/NEJM199808203390806","article-title":"Staphylococcus aureus infections.","volume":"339","author":"FD Lowy","year":"1998","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1099\/00222615-28-4-249","article-title":"Adherence of staphylococci to intravascular catheters.","volume":"28","author":"KG Kristinsson","year":"1989","journal-title":"J Med Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ijantimicag.2005.11.004","article-title":"Community-acquired methicillin-resistant Staphylococcus aureus infections.","volume":"27","author":"HC Maltezou","year":"2006","journal-title":"Int J Antimicrob Agents"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"S269","DOI":"10.1086\/430788","article-title":"Community-associated methicillin-resistant Staphylococcus aureus.","volume":"41","author":"JT Weber","year":"2005","journal-title":"Clin Infect Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1038\/nrmicro1289","article-title":"Immune evasion by staphylococci.","volume":"3","author":"TJ Foster","year":"2005","journal-title":"Nat Rev Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1128\/CMR.13.1.16-34.2000","article-title":"Exotoxins of Staphylococcus aureus.","volume":"13","author":"MM Dinges","year":"2000","journal-title":"Clin Microbiol Rev"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1128\/CMR.17.1.218-234.2004","article-title":"Staphylococcus aureus capsular polysaccharides.","volume":"17","author":"K O'Riordan","year":"2004","journal-title":"Clin Microbiol Rev"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/S0966-842X(98)01400-0","article-title":"Surface protein adhesins of Staphylococcus aureus.","volume":"6","author":"TJ Foster","year":"1998","journal-title":"Trends Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1046\/j.1365-2958.2002.02935.x","article-title":"Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus aureus: roles for the clumping factors ClfA and ClfB, the serine-aspartate repeat protein SdrE and protein A.","volume":"44","author":"L O'Brien","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1111\/j.1432-1033.1997.00416.x","article-title":"Characterization of the interaction between the Staphylococcus aureus clumping factor (ClfA) and fibrinogen.","volume":"247","author":"D McDevitt","year":"1997","journal-title":"Eur J Biochem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"4987","DOI":"10.1128\/IAI.70.9.4987-4996.2002","article-title":"Virulent combinations of adhesin and toxin genes in natural populations of Staphylococcus aureus.","volume":"70","author":"SJ Peacock","year":"2002","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1086\/324430","article-title":"Protection against experimental Staphylococcus aureus arthritis by vaccination with clumping factor A, a novel virulence determinant.","volume":"184","author":"E Josefsson","year":"2001","journal-title":"J Infect Dis"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"3120","DOI":"10.1128\/IAI.69.5.3120-3127.2001","article-title":"Clumping factor A mediates binding of Staphylococcus aureus to human platelets.","volume":"69","author":"IR Siboo","year":"2001","journal-title":"Infect Immun"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"4915","DOI":"10.1128\/IAI.64.12.4915-4921.1996","article-title":"Diminished platelet binding in vitro by Staphylococcus aureus is associated with reduced virulence in a rabbit model of infective endocarditis.","volume":"64","author":"PM Sullam","year":"1996","journal-title":"Infect Immun"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"6296","DOI":"10.1128\/IAI.69.10.6296-6302.2001","article-title":"Reassessing the role of Staphylococcus aureus clumping factor and fibronectin-binding protein by expression in Lactococcus lactis.","volume":"69","author":"YA Que","year":"2001","journal-title":"Infect Immun"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"6864","DOI":"10.1128\/IAI.71.12.6864-6870.2003","article-title":"Characterization of a protective monoclonal antibody recognizing Staphylococcus aureus MSCRAMM protein clumping factor A.","volume":"71","author":"AE Hall","year":"2003","journal-title":"Infect Immun"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"5229","DOI":"10.1128\/IAI.73.8.5229-5232.2005","article-title":"Characterization of a humanized monoclonal antibody recognizing clumping factor A expressed by Staphylococcus aureus.","volume":"73","author":"PJ Domanski","year":"2005","journal-title":"Infect Immun"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"S39","DOI":"10.1016\/j.vaccine.2004.08.015","article-title":"A humanized monoclonal antibody targeting Staphylococcus aureus.","volume":"22","author":"JM Patti","year":"2004","journal-title":"Vaccine"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1128\/MMBR.70.1.192-221.2006","article-title":"Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria.","volume":"70","author":"LA Marraffini","year":"2006","journal-title":"Microbiol Mol Biol Rev"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1046\/j.1365-2958.2001.02411.x","article-title":"Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus.","volume":"40","author":"SK Mazmanian","year":"2001","journal-title":"Mol Microbiol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/j.1365-2958.1994.tb00304.x","article-title":"Molecular characterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus.","volume":"11","author":"D McDevitt","year":"1994","journal-title":"Mol Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1046\/j.1365-2958.1998.01050.x","article-title":"Clumping factor B (ClfB), a new surface-located fibrinogen-binding adhesin of Staphylococcus aureus.","volume":"30","author":"D Ni Eidhin","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"13863","DOI":"10.1074\/jbc.275.18.13863","article-title":"The fibronectin-binding MSCRAMM FnbpA of Staphylococcus aureus is a bifunctional protein that also binds to fibrinogen.","volume":"275","author":"ER Wann","year":"2000","journal-title":"J Biol Chem"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"6660","DOI":"10.1093\/emboj\/cdf619","article-title":"A novel variant of the immunoglobulin fold in surface adhesins of Staphylococcus aureus: crystal structure of the fibrinogen-binding MSCRAMM, clumping factor A.","volume":"21","author":"CC Deivanayagam","year":"2002","journal-title":"Embo J"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0092-8674(03)00809-2","article-title":"A \u201cdock, lock, and latch\u201d structural model for a staphylococcal adhesin binding to fibrinogen.","volume":"115","author":"K Ponnuraj","year":"2003","journal-title":"Cell"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"2915","DOI":"10.1021\/bi00433a025","article-title":"Platelet receptor recognition domain on the gamma chain of human fibrinogen and its synthetic peptide analogues.","volume":"28","author":"M Kloczewiak","year":"1989","journal-title":"Biochemistry"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"10729","DOI":"10.1073\/pnas.89.22.10729","article-title":"Role of fibrinogen alpha and gamma chain sites in platelet aggregation.","volume":"89","author":"DH Farrell","year":"1992","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1055\/s-0038-1646347","article-title":"The residues AGDV of recombinant gamma chains of human fibrinogen must be carboxy-terminal to support human platelet aggregation.","volume":"68","author":"JM Hettasch","year":"1992","journal-title":"Thromb Haemost"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1160\/TH05-03-0205","article-title":"ClfA(221\u2013550), a fibrinogen-binding segment of Staphylococcus aureus clumping factor A, disrupts fibrinogen function.","volume":"94","author":"CZ Liu","year":"2005","journal-title":"Thromb Haemost"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.thromres.2007.03.019","article-title":"A segment of Staphylococcus aureus clumping factor A with fibrinogen-binding activity (ClfA221\u2013550) inhibits platelet-plug formation in mice.","volume":"121","author":"CZ Liu","year":"2007","journal-title":"Thromb Res"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1074\/jbc.M706252200","article-title":"Evidence for the \u201cdock, lock, and latch\u201d ligand binding mechanism of the staphylococcal microbial surface component recognizing adhesive matrix molecules (MSCRAMM) SdrG.","volume":"283","author":"MG Bowden","year":"2008","journal-title":"J Biol Chem"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"6821","DOI":"10.1074\/jbc.273.12.6821","article-title":"The fibrinogen-binding MSCRAMM (clumping factor) of Staphylococcus aureus has a Ca2+-dependent inhibitory site.","volume":"273","author":"DP O'Connell","year":"1998","journal-title":"J Biol Chem"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1111\/j.1365-2958.1995.tb02316.x","article-title":"Identification of the ligand-binding domain of the surface-located fibrinogen receptor (clumping factor) of Staphylococcus aureus.","volume":"16","author":"D McDevitt","year":"1995","journal-title":"Mol Microbiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nature02976","article-title":"Structural basis for allostery in integrins and binding to fibrinogen-mimetic therapeutics.","volume":"432","author":"T Xiao","year":"2004","journal-title":"Nature"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1126\/science.1069040","article-title":"Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand.","volume":"296","author":"JP Xiong","year":"2002","journal-title":"Science"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"4224","DOI":"10.1038\/sj.emboj.7600888","article-title":"A \u2018Collagen Hug\u2019 model for Staphylococcus aureus CNA binding to collagen.","volume":"24","author":"Y Zong","year":"2005","journal-title":"Embo J"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1038\/342224a0","article-title":"Protein structure. Chaperones, paperones.","volume":"342","author":"J Sambrook","year":"1989","journal-title":"Nature"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.1074\/jbc.M007979200","article-title":"Identification of residues in the Staphylococcus aureus fibrinogen-binding MSCRAMM clumping factor A (ClfA) that are important for ligand binding.","volume":"276","author":"OM Hartford","year":"2001","journal-title":"J Biol Chem"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/0378-1119(89)90358-2","article-title":"Site-directed mutagenesis by overlap extension using the polymerase chain reaction.","volume":"77","author":"SN Ho","year":"1989","journal-title":"Gene"},{"key":"ref40","first-page":"528","article-title":"Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.","volume":"8","author":"RM Horton","year":"1990","journal-title":"Biotechniques"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"1718","DOI":"10.1107\/S090744499900935X","article-title":"The finer things in X-ray diffraction data collection.","volume":"55","author":"JW Pflugrath","year":"1999","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1107\/S0907444905001617","article-title":"Likelihood-enhanced fast translation functions.","volume":"61","author":"AJ McCoy","year":"2005","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics.","volume":"60","author":"P Emsley","year":"2004","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1107\/S0907444901014007","article-title":"ARP\/wARP and molecular replacement.","volume":"57","author":"A Perrakis","year":"2001","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1107\/S0907444996012255","article-title":"Refinement of macromolecular structures by the maximum-likelihood method.","volume":"53","author":"GN Murshudov","year":"1997","journal-title":"Acta Crystallogr D Biol Crystallogr"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1006\/jmbi.1993.1351","article-title":"Main-chain bond lengths and bond angles in protein structures.","volume":"231","author":"RA Laskowski","year":"1993","journal-title":"J Mol Biol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0263-7855(87)80010-3","article-title":"Ribbon models for macromolecules.","volume":"5","author":"MJ Carson","year":"1997","journal-title":"J Mol Graph"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1000226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,21]],"date-time":"2023-05-21T17:07:31Z","timestamp":1684688851000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1000226"}},"subtitle":[],"editor":[{"given":"Theresa","family":"Koehler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2008,11,28]]},"references-count":47,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2008,11,28]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1000226","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11,28]]}}}