{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:42:51Z","timestamp":1791297771391,"version":"4.1.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T00:00:00Z","timestamp":1352937600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1002983","type":"journal-article","created":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T22:39:11Z","timestamp":1353019151000},"page":"e1002983","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":51,"title":["Brucella \u03b2 1,2 Cyclic Glucan Is an Activator of Human and Mouse Dendritic Cells"],"prefix":"10.1371","volume":"8","author":[{"given":"Anna","family":"Martirosyan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camino","family":"P\u00e9rez-Gutierrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romain","family":"Banchereau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Dutartre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Lecine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Dullaers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marielle","family":"Mello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana","family":"Pinto Salcedo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Muller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Leserman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Levy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Zurawski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandy","family":"Zurawski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Moriy\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eynav","family":"Klechevsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Banchereau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SangKon","family":"Oh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Gorvel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2012,11,15]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1128\/MMBR.58.2.145-161.1994","article-title":"Cyclic beta-glucans of members of the family Rhizobiaceae","volume":"58","author":"MW Breedveld","year":"1994","journal-title":"Microbiol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1600-065X.2010.00982.x","article-title":"An evolutionary strategy for a stealthy intracellular Brucella pathogen","volume":"240","author":"A Martirosyan","year":"2011","journal-title":"Immunol Rev"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0378-1135(02)00214-6","article-title":"Brucella intracellular life: from invasion to intracellular replication","volume":"90","author":"JP Gorvel","year":"2002","journal-title":"Vet Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.1128\/JB.180.17.4392-4400.1998","article-title":"Molecular cloning and characterization of cgs, the Brucella abortus cyclic beta(1\u20132) glucan synthetase gene: genetic complementation of Rhizobium meliloti ndvB and Agrobacterium tumefaciens chvB mutants","volume":"180","author":"N Inon de Iannino","year":"1998","journal-title":"J Bacteriol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"4528","DOI":"10.1128\/IAI.69.7.4528-4535.2001","article-title":"Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication in HeLa cells","volume":"69","author":"G Briones","year":"2001","journal-title":"Infect Immun"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/ni1202","article-title":"Cyclic beta-1,2-glucan is a Brucella virulence factor required for intracellular survival","volume":"6","author":"B Arellano-Reynoso","year":"2005","journal-title":"Nat Immunol"},{"key":"ref7","first-page":"9023","article-title":"Beta-glucan functions as an adjuvant for monoclonal antibody immunotherapy by recruiting tumoricidal granulocytes as killer cells","volume":"63","author":"F Hong","year":"2003","journal-title":"Cancer Res"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0162-3109(99)00013-2","article-title":"Therapeutic intervention with complement and beta-glucan in cancer","volume":"42","author":"GD Ross","year":"1999","journal-title":"Immunopharmacology"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"2374","DOI":"10.2527\/jas.2004-541","article-title":"Effects of beta-glucan extracted from Saccharomyces cerevisiae on growth performance, and immunological and somatotropic responses of pigs challenged with Escherichia coli lipopolysaccharide","volume":"84","author":"J Li","year":"2006","journal-title":"J Anim Sci"},{"key":"ref10","first-page":"111","article-title":"Protective effect of beta-glucan against systemic Streptococcus pneumoniae infection in mice","volume":"27","author":"G Hetland","year":"2000","journal-title":"FEMS Immunol Med Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1046\/j.1365-3083.1998.00350.x","article-title":"Protective effect of beta-glucan against mycobacterium bovis, BCG infection in BALB\/c mice","volume":"47","author":"G Hetland","year":"1998","journal-title":"Scand J Immunol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/S0192-0561(98)00007-1","article-title":"Enhanced clearance of a multiple antibiotic resistant Staphylococcus aureus in rats treated with PGG-glucan is associated with increased leukocyte counts and increased neutrophil oxidative burst activity","volume":"20","author":"J Liang","year":"1998","journal-title":"Int J Immunopharmacol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1462-5822.2005.00505.x","article-title":"Immune recognition of fungal beta-glucans","volume":"7","author":"GD Brown","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1600-065X.2009.00793.x","article-title":"Beta-glucan recognition by the innate immune system","volume":"230","author":"HS Goodridge","year":"2009","journal-title":"Immunol Rev"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.coi.2009.01.004","article-title":"Mechanism of action of clinically approved adjuvants","volume":"21","author":"BN Lambrecht","year":"2009","journal-title":"Current opinion in immunology"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.0818-9641.2004.01272.x","article-title":"Vaccine adjuvants: current state and future trends","volume":"82","author":"N Petrovsky","year":"2004","journal-title":"Immunology and cell biology"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"6777","DOI":"10.1016\/j.vaccine.2008.09.045","article-title":"Engagement of TLR signaling as adjuvant: towards smarter vaccine and beyond","volume":"26","author":"A Lahiri","year":"2008","journal-title":"Vaccine"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.coi.2009.05.003","article-title":"Immunology of TLR-independent vaccine adjuvants","volume":"21","author":"E De Gregorio","year":"2009","journal-title":"Curr Opin Immunol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1371\/journal.ppat.0040021","article-title":"Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1","volume":"4","author":"SP Salcedo","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1574-6968.2000.tb09075.x","article-title":"Osmoregulated periplasmic glucans in Proteobacteria","volume":"186","author":"JP Bohin","year":"2000","journal-title":"FEMS Microbiol Lett"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.1128\/JB.00086-06","article-title":"The Brucella abortus cyclic beta-1,2-glucan virulence factor is substituted with O-ester-linked succinyl residues","volume":"188","author":"MS Roset","year":"2006","journal-title":"J Bacteriol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"5771","DOI":"10.1074\/jbc.M511461200","article-title":"Ligands for the beta-glucan receptor, Dectin-1, assigned using \u201cdesigner\u201d microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides","volume":"281","author":"AS Palma","year":"2006","journal-title":"J Biol Chem"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/nature04926","article-title":"Card9 controls a non-TLR signalling pathway for innate anti-fungal immunity","volume":"442","author":"O Gross","year":"2006","journal-title":"Nature"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1002\/eji.200324610","article-title":"Viral infection and Toll-like receptor agonists induce a differential expression of type I and lambda interferons in human plasmacytoid and monocyte-derived dendritic cells","volume":"34","author":"EM Coccia","year":"2004","journal-title":"Eur J Immunol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1535","DOI":"10.4049\/jimmunol.173.3.1535","article-title":"Regulation of IFN regulatory factor-7 and IFN-alpha production by enveloped virus and lipopolysaccharide in human plasmacytoid dendritic cells","volume":"173","author":"J Dai","year":"2004","journal-title":"J Immunol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1189\/jlb.0809543","article-title":"Technical advance: Generation of human pDC equivalents from primary monocytes using Flt3-L and their functional validation under hypoxia","volume":"88","author":"D Sekar","year":"2010","journal-title":"J Leukoc Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1038\/nrmicro1068","article-title":"LPS, TLR4 and infectious disease diversity","volume":"3","author":"SI Miller","year":"2005","journal-title":"Nature Rev Microbiol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.immuni.2008.07.013","article-title":"Functional specializations of human epidermal Langerhans cells and CD14+ dermal dendritic cells","volume":"29","author":"E Klechevsky","year":"2008","journal-title":"Immunity"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1182\/blood-2010-01-264960","article-title":"Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR","volume":"116","author":"E Klechevsky","year":"2010","journal-title":"Blood"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.smim.2011.01.004","article-title":"Targeting human dendritic cell subsets for improved vaccines","volume":"23","author":"H Ueno","year":"2011","journal-title":"Seminars in immunology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/ni.2039","article-title":"Immunological mechanisms of vaccination","volume":"12","author":"B Pulendran","year":"2011","journal-title":"Nature Immunol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0092-8674(01)00449-4","article-title":"Dendritic cells: specialized and regulated antigen processing machines","volume":"106","author":"I Mellman","year":"2001","journal-title":"Cell"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1038\/nri1246","article-title":"Dendritic-cell control of pathogen-driven T-cell polarization","volume":"3","author":"ML Kapsenberg","year":"2003","journal-title":"Nature Rev Immunol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"3576","DOI":"10.1128\/IAI.00022-11","article-title":"The Toll-like receptor 4 agonist monophosphoryl lipid a augments innate host resistance to systemic bacterial infection","volume":"79","author":"CD Romero","year":"2011","journal-title":"Infect Immun"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.imlet.2009.04.002","article-title":"Induction of tolerogenic vs immunogenic dendritic cells (DCs) in the presence of GM-CSF is regulated by the strength of signaling from monophosphoryl lipid A (MPLA) in association with glutathione and fetal hemoglobin gamma-chain","volume":"124","author":"I Khatri","year":"2009","journal-title":"Immunol Lett"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"6205","DOI":"10.4049\/jimmunol.1200255","article-title":"Dynamic Roles of Type I and Type II IFNs in Early Infection with Mycobacterium tuberculosis","volume":"188","author":"L Desvignes","year":"2012","journal-title":"J Immunol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.jneuroim.2010.08.008","article-title":"Intrauterine infection\/inflammation and perinatal brain damage: role of glial cells and Toll-like receptor signaling","volume":"229","author":"TM Yuan","year":"2010","journal-title":"J Neuroimmunol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.4049\/jimmunol.178.3.1243","article-title":"TLR4, ethanol, and lipid rafts: a new mechanism of ethanol action with implications for other receptor-mediated effects","volume":"178","author":"G Szabo","year":"2007","journal-title":"J Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1002\/eji.200939992","article-title":"CD14-independent responses induced by a synthetic lipid A mimetic","volume":"40","author":"A Legat","year":"2010","journal-title":"Eur J Immunol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1002\/eji.200535777","article-title":"Pathogen-specific TLR signaling in mucosa: mutual contribution of microbial TLR agonists and virulence factors","volume":"36","author":"JC Sirard","year":"2006","journal-title":"Eur J Immunol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1038\/417177a","article-title":"Transient aggregation of ubiquitinated proteins during dendritic cell maturation","volume":"417","author":"H Lelouard","year":"2002","journal-title":"Nature"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1128\/jb.178.4.1070-1079.1996","article-title":"Characterization of Brucella abortus and Brucella melitensis native haptens as outer membrane O-type polysaccharides independent from the smooth lipopolysaccharide","volume":"178","author":"V Aragon","year":"1996","journal-title":"J Bacteriol"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1084\/jem.176.6.1693","article-title":"Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte\/macrophage colony-stimulating factor","volume":"176","author":"K Inaba","year":"1992","journal-title":"J Exp Med"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.1073\/pnas.0500014102","article-title":"Selective blockade of inhibitory Fcgamma receptor enables human dendritic cell maturation with IL-12p70 production and immunity to antibody-coated tumor cells","volume":"102","author":"KM Dhodapkar","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"16492","DOI":"10.1073\/pnas.0708025104","article-title":"A glycosyltransferase with a length-controlling activity as a mechanism to regulate the size of polysaccharides","volume":"104","author":"AE Ciocchini","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e1002675","DOI":"10.1371\/journal.ppat.1002675","article-title":"The Lipopolysaccharide Core of Brucella abortus Acts as a Shield Against Innate Immunity Recognition","volume":"8","author":"R Conde-\u00c1lvarez","year":"2012","journal-title":"PLoS Pathog"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1128\/IAI.31.1.214-222.1981","article-title":"Immunochemical characterization of Brucella lipopolysaccharides and polysaccharides","volume":"31","author":"E Moreno","year":"1981","journal-title":"Infect Immun"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1002983","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T04:57:11Z","timestamp":1594875431000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1002983"}},"subtitle":[],"editor":[{"given":"Ren\u00e9e M.","family":"Tsolis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2012,11,15]]},"references-count":47,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2012,11,15]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1002983","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,15]]}}}