{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:02:13Z","timestamp":1791201733781,"version":"4.2.0"},"reference-count":70,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2013,5,9]],"date-time":"2013-05-09T00:00:00Z","timestamp":1368057600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1003354","type":"journal-article","created":{"date-parts":[[2013,5,9]],"date-time":"2013-05-09T20:58:37Z","timestamp":1368133117000},"page":"e1003354","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":79,"title":["Antigenic Drift of the Pandemic 2009 A(H1N1) Influenza Virus in a Ferret Model"],"prefix":"10.1371","volume":"9","author":[{"given":"Teagan","family":"Guarnaccia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise A.","family":"Carolan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Maurer-Stroh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael T. C.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Job","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick C.","family":"Reading","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Petrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James M.","family":"McCaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodie","family":"McVernon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aeron C.","family":"Hurt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Kelso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Mosse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian G.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen L.","family":"Laurie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,5,9]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2605","DOI":"10.1056\/NEJMoa0903810","article-title":"Emergence of a novel swine-origin influenza A (H1N1) virus in humans","volume":"360","author":"FS Dawood","year":"2009","journal-title":"N Engl J Med"},{"key":"ref2","unstructured":"WHO (2012) Recommended composition of influenza virus vaccines for use in the 2012\u20132013 northern hemisphere influenza season. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/influenza\/vaccines\/virus\/recommendations\/201202_recommendation.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/influenza\/vaccines\/virus\/recommendations\/201202_recommendation.pdf<\/ext-link> Accessed 19 September 2012."},{"key":"ref3","unstructured":"WHO (2012) Recommended composition of influenza virus vaccines for use in the 2013 southern hemisphere influenza season. Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/influenza\/vaccines\/virus\/recommendations\/201209_recommendation.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/influenza\/vaccines\/virus\/recommendations\/201209_recommendation.pdf<\/ext-link> Accessed 19 September 2012."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e21828","DOI":"10.1371\/journal.pone.0021828","article-title":"The age-specific cumulative incidence of infection with pandemic influenza H1N1 2009 was similar in various countires prior to vaccination","volume":"6","author":"H Kelly","year":"2011","journal-title":"PLoS One"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1111\/j.1462-5822.2010.01475.x","article-title":"Evasion of innate and adaptive immune responses by influenza A virus","volume":"12","author":"M Schmolke","year":"2010","journal-title":"Cell Microbiol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/0092-8674(82)90135-0","article-title":"The antigenic structure of the influenza virus A\/PR\/8\/34 hemagglutinin (H1 subtype)","volume":"31","author":"AJ Caton","year":"1982","journal-title":"Cell"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/290713a0","article-title":"Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies","volume":"290","author":"W Gerhard","year":"1981","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1146\/annurev.mi.35.100181.001153","article-title":"Antigenic characterization of viruses by monoclonal antibodies","volume":"35","author":"JW Yewdell","year":"1981","journal-title":"Annu Rev Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1128\/JVI.48.1.52-60.1983","article-title":"Antigenic drift in influenza virus H3 hemagglutinin from 1968 to 1980: multiple evolutionary pathways and sequential amino acid changes at key antigenic sites","volume":"48","author":"GW Both","year":"1983","journal-title":"Journal of Virology"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1073\/pnas.81.6.1779","article-title":"A carbohydrate side chain on hemagglutinins of Hong Kong influenza viruses inhibits recognition by a monoclonal antibody","volume":"81","author":"JJ Skehel","year":"1984","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1080\/08830180802593540","article-title":"Neutralizing anti-influenza virus monoclonal antibodies: therapeutics and tools for discovery","volume":"28","author":"O Martinez","year":"2009","journal-title":"International Reviews of Immunology"},{"key":"ref12","first-page":"44","article-title":"Identification of genetic diversity by cultivating influenza A(H3N2) virus in vitro in the presence of post-infection sera from small children","volume":"10","author":"LR Haaheim","year":"2006","journal-title":"Vaccine"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1126\/science.1178258","article-title":"Hemagglutinin receptor binding avidity drives influenza A virus antigenic drift","volume":"326","author":"SE Hensley","year":"2009","journal-title":"Science"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"5329","DOI":"10.1128\/JVI.02469-09","article-title":"Intrahost evolutionary dynamics of canine influenza virus in naive and partially immune dogs","volume":"84","author":"K Hoelzer","year":"2010","journal-title":"Journal of Virology"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"e00120","DOI":"10.1128\/mBio.00120-12","article-title":"Antibody pressure by a human monoclonal antibody targeting the 2009 pandemic H1N1 virus hemagglutinin drives the emergence of a virus with increased virulence in mice","volume":"3","author":"CD O'Donnell","year":"2012","journal-title":"mBio"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.virusres.2012.03.003","article-title":"Escape mutants of pandemic influenza A\/H1N1 2009 virus: variations in antigenic specificity and receptor affinity of the hemagglutinin","volume":"166","author":"I Rudneva","year":"2012","journal-title":"Virus Research"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"e20130","DOI":"10.1371\/journal.pone.0020130","article-title":"Evolution of H3N2 influenza virus in a guinea pig model","volume":"6","author":"J Long","year":"2011","journal-title":"PLoS One"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"6943","DOI":"10.1128\/JVI.00112-10","article-title":"Intra- and interhost evolutionary dynamics of equine influenza virus","volume":"84","author":"PR Murcia","year":"2010","journal-title":"Journal of Virology"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1146\/annurev.iy.08.040190.003513","article-title":"Structural basis of immune recognition of influenza virus hemagglutinin","volume":"8","author":"I Wilson","year":"1990","journal-title":"Annual review of immunology"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/JVI.57.1.124-128.1986","article-title":"Comparative analyses of the specificities of anti-influenza hemagglutinin antibodies in human sera","volume":"57","author":"M Wang","year":"1986","journal-title":"Journal of Virology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e27517","DOI":"10.1371\/journal.pone.0027517","article-title":"Decoding the distribution of glycan receptors for human-adapted influenza A viruses in ferret respiratory tract","volume":"7","author":"A Jayaraman","year":"2012","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1242\/dmm.007823","article-title":"The ferret as a model organism to study influenza A virus infection","volume":"4","author":"JA Belser","year":"2011","journal-title":"Disease Models & Mechanisms"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.micinf.2011.01.014","article-title":"The contribution of animal models to the understanding of the host range and virulence of influenza A viruses","volume":"13","author":"CD O'Donnell","year":"2011","journal-title":"Microbes and Infection"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1086\/656188","article-title":"Multiple infections with seasonal influenza A virus induce cross-protective immunity against A(H1N1) pandemic influenza virus in a ferret model","volume":"202","author":"KL Laurie","year":"2010","journal-title":"Journal of Infectious Diseases"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3618","DOI":"10.1016\/j.vaccine.2012.03.043","article-title":"Control of pandemic (H1N1) 2009 influenza virus infection of ferret lungs by non-adjuvant-containing pandemic and seasonal vaccines","volume":"30","author":"S Rockman","year":"2012","journal-title":"Vaccine"},{"key":"ref26","unstructured":"World Health Organization GIP (2002) WHO Manual on Animal Influenza Diagnosis and Surveillance. WHO\/CDS\/CSR\/NCS\/2002.5 Rev.1."},{"key":"ref27","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1038\/292072a0","article-title":"Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype","volume":"292","author":"G Winter","year":"1981","journal-title":"Nature"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/nbt1375","article-title":"Glycan topology determines human adaptation of avian H5N1 virus hemagglutinin","volume":"26","author":"A Chandrasekaran","year":"2008","journal-title":"Nature Biotechnology"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1128\/JVI.02106-09","article-title":"Generation of live attenuated novel influenza virus A\/California\/7\/09 (H1N1) vaccines with high yield in embryonated chicken eggs","volume":"84","author":"X Chen","year":"2010","journal-title":"Journal of Virology"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"11533","DOI":"10.1128\/JVI.79.17.11533-11536.2005","article-title":"A single amino acid substitution in 1918 influenza virus hemagglutinin changes receptor binding specificity","volume":"79","author":"L Glaser","year":"2005","journal-title":"Journal of Virology"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"e8553","DOI":"10.1371\/journal.pone.0008553","article-title":"Predicting the antigenic structure of the pandemic (H1N1) 2009 influenza virus hemagglutinin","volume":"5","author":"M Igarashi","year":"2010","journal-title":"PLoS One"},{"key":"ref32","unstructured":"Coleman PM (1998) Textbook of Influenza; Nicholson KG, Webster RG, Hay AJ, editors. UK: Blackwell Science."},{"key":"ref33","doi-asserted-by":"crossref","first-page":"9427","DOI":"10.1128\/JVI.00373-10","article-title":"Assessing the viral fitness of oseltamivir-resistant influenza viruses in ferrets, using a competitive-mixtures model","volume":"84","author":"AC Hurt","year":"2010","journal-title":"Journal of Virology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.coviro.2012.03.003","article-title":"The role of receptor binding specificity in interspecies transmission of influenza viruses","volume":"2","author":"M Imai","year":"2012","journal-title":"Current Opinion in Virology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/s10719-010-9303-4","article-title":"Glycans as receptors for influenza pathogenesis","volume":"27","author":"K Viswanathan","year":"2010","journal-title":"Glycoconjugate Journal"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"8418","DOI":"10.1128\/JVI.77.15.8418-8425.2003","article-title":"Overexpression of the a-2,6-Sialyltransferase in MDCK Cells Increases Influenza Virus Sensitivity to Neuraminidase Inhibitors","volume":"77","author":"M Matrosovich","year":"2003","journal-title":"Journal of Virology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1083\/jcb.114.3.401","article-title":"Folding of influenza hemagglutinin in the endoplasmic reticulum","volume":"114","author":"I Braakman","year":"1991","journal-title":"Journal of Cell Biology"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"9808","DOI":"10.1073\/pnas.0903815106","article-title":"Temperature sensitivity of human perforin mutants unmasks subtotal loss of cytotoxicity, delayed FHL, and a predisposition to cancer","volume":"106","author":"J Chia","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"6769","DOI":"10.1128\/JVI.00458-10","article-title":"Neuraminidase receptor binding variants of human influenza A(H3N2) viruses resulting from substitution of aspartic acid 151 in the catalytic siteL a role in virus attachment?","volume":"84","author":"YP Lin","year":"2010","journal-title":"Journal of Virology"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e25848","DOI":"10.1371\/journal.pone.0025848","article-title":"Minor Changes in the Hemagglutinin of Influenza A(H1N1)2009 Virus Alter Its Antigenic Properties","volume":"6","author":"M Strengell","year":"2011","journal-title":"PLoS One"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"4421","DOI":"10.1128\/JVI.02356-10","article-title":"Effects of receptor binding specificity of avian influenza virus on the human innate immune response","volume":"85","author":"I Ramos","year":"2011","journal-title":"Journal of Virology"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.virusres.2005.05.005","article-title":"Receptor-binding properties of swine influenza viruses isolated and propagated in MDCK cells","volume":"114","author":"AS Gambaryan","year":"2005","journal-title":"Virus Research"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1743-422X-4-42","article-title":"Receptor binding specificity of recent human H3N2 influenza viruses","volume":"4","author":"K Kumari","year":"2007","journal-title":"Virology Journal"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1016\/j.jmb.2005.11.002","article-title":"Glycan microarray analysis of the hemagglutinins from modern and pandemic influenza viruses reveals difference receptor specificities","volume":"355","author":"J Stevens","year":"2006","journal-title":"Journal of Molecular Biology"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"e13768","DOI":"10.1371\/journal.pone.0013768","article-title":"Determinants of glycan receptor specificity of H2N2 influenza A virus hemagglutinin","volume":"5","author":"K Viswanathan","year":"2010","journal-title":"PLoS One"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/viro.2001.1320","article-title":"An antibody that prevents the hemagglutinin low pH fusogenic transition","volume":"294","author":"C Barbey-Martin","year":"2002","journal-title":"Virology"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/nsb0298-119","article-title":"Antigen distortion allows influenza virus to escape neutralization","volume":"5","author":"D Fleury","year":"1998","journal-title":"Nature Structural Biology"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1126\/science.1186430","article-title":"Structural basis of preexisting immunity to the 2009 H1N1 pandemic influenza virus","volume":"328","author":"R Xu","year":"2010","journal-title":"Science"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"93ps32","DOI":"10.1126\/scitranslmed.3002694","article-title":"Antibody Repertoire: Embracing Diversity","volume":"3","author":"AJ Pollard","year":"2011","journal-title":"Science Translational Medicine"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"21474","DOI":"10.1073\/pnas.1218841110","article-title":"Evolution of the receptor binding properties of the influenza A(H3N2) hemagglutinin","volume":"109","author":"YP Lin","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"RRN1152","DOI":"10.1371\/currents.RRN1152","article-title":"Structure and Receptor binding properties of a pandemic H1N1 virus hemagglutinin","volume":"2","author":"H Yang","year":"2010","journal-title":"Plos Currents"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1128\/JCM.00398-08","article-title":"MDCK-SIAT1 cells show improved isolation rates for recent human influenza viruses compared to conventional MDCK cells","volume":"46","author":"DY Oh","year":"2008","journal-title":"J Clin Microbiol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"e1002730","DOI":"10.1371\/journal.ppat.1002730","article-title":"Evolution of an Eurasian avian-like influenza virus in na\u00efve and vaccinated pigs","volume":"8","author":"PR Murcia","year":"2012","journal-title":"PLoS Pathogens"},{"key":"ref54","unstructured":"WHO (2009) CDC protocol of realtime RTPCR for swine influenza A(H1N1) Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/CDCrealtimeRTPCRprotocol_20090428.pdf\" xlink:type=\"simple\">http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/CDCrealtimeRTPCRprotocol_20090428.pdf<\/ext-link> Accessed 4 April 2013."},{"key":"ref55","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1007\/s007050170002","article-title":"Universal primer set for the full-length amplification of all influenza A viruses","volume":"146","author":"E Hoffmann","year":"2001","journal-title":"Archives of Virology"},{"key":"ref56","unstructured":"WHO (2009) Sequencing primers and protocol Available: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/sequencing_primers\/en\/index.html\" xlink:type=\"simple\">http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/sequencing_primers\/en\/index.html<\/ext-link> Accessed 4 April 2013."},{"key":"ref57","doi-asserted-by":"crossref","first-page":"6108","DOI":"10.1073\/pnas.100133697","article-title":"A DNA transfection system for generation of influenza A virus from eight plasmids","volume":"97","author":"E Hoffmann","year":"2000","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"e23400","DOI":"10.1371\/journal.pone.0023400","article-title":"Rapid detection and subtyping of human influenza A viruses and reassortants by pyrosequencing","volume":"6","author":"YM Deng","year":"2011","journal-title":"PLoS One"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1007\/s00439-002-0722-6","article-title":"Estimation of single nucleotide polymorphism allele frequency in DNA pools by using Pyrosequencing","volume":"110","author":"JD Gruber","year":"2002","journal-title":"Human Genetics"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"2612","DOI":"10.1128\/JCM.00449-06","article-title":"Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency","volume":"44","author":"EK Halvas","year":"2006","journal-title":"Journal of Clincal Microbiology"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"2573","DOI":"10.1038\/nprot.2006.442","article-title":"Single nucleotide polymorphism (SNP) allele frequency estimation in DNA pools using pyrosequencing","volume":"1","author":"C Lavebratt","year":"2006","journal-title":"Nature Protocols"},{"key":"ref62","unstructured":"CDC (1982) Concepts and Procedures for Laboratory-Based Influenza Surveillance. Kendal A PM, Skehel J, editor. Atlanta, GA: US Department of Health and Human Services, CDC. 8 p."},{"key":"ref63","doi-asserted-by":"crossref","first-page":"19678","DOI":"10.2807\/ese.15.40.19678-en","article-title":"Seroprevalence of 2009 pandemic influenza A(H1N1) virus in Australian blood donors, October\u2013December 2009","volume":"15","author":"J McVernon","year":"2010","journal-title":"Eurosurveillance"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1093\/bib\/bbn013","article-title":"Recent developments in the MAFFT multiple sequence alignment program","volume":"9","author":"K Katoh","year":"2008","journal-title":"Briefings in Bioinformatics"},{"key":"ref65","unstructured":"Monson R R (1990) Occupational Epidemiology. Boca Raton, FL: CRC Press."},{"key":"ref66","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1128\/JVI.06322-11","article-title":"Structural characterization of the hemagglutinin receptor specificity from the 2009 H1N1 influenza pandemic","volume":"86","author":"R Xu","year":"2012","journal-title":"Journal of Virology"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1002\/prot.10104","article-title":"Increasing the precision of comparative models with YASARA NOVA-a self-parameterizing force field","volume":"47","author":"E Krieger","year":"2002","journal-title":"Proteins"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1002\/prot.22570","article-title":"Improving physical realism, stereochemistry, and side-chain accuracy in homology modeling: Four approaches that performed well in CASP8","volume":"77","author":"E Krieger","year":"2009","journal-title":"Proteins"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1002\/jcc.10349","article-title":"A point-charge force field for molecular mechanics simulations of proteins based on condensed-phase quantum mechanical calculations","volume":"24","author":"Y Duan","year":"2003","journal-title":"Journal of Computational Chemistry"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"8715","DOI":"10.1073\/pnas.91.18.8715","article-title":"Atomic-level accuracy in simulations of large protein crystals","volume":"91","author":"DM York","year":"1994","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1003354","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,26]],"date-time":"2020-07-26T14:43:24Z","timestamp":1595774604000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1003354"}},"subtitle":[],"editor":[{"given":"Kanta","family":"Subbarao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,5,9]]},"references-count":70,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,5,9]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1003354","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,9]]}}}