{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:19:25Z","timestamp":1791292765533,"version":"4.1.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T00:00:00Z","timestamp":1377734400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1003589","type":"journal-article","created":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T22:03:04Z","timestamp":1377813784000},"page":"e1003589","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":57,"title":["Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2"],"prefix":"10.1371","volume":"9","author":[{"given":"Ryosuke","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mami","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Watashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Aizaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiharu","family":"Matsuura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaji","family":"Wakita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuro","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"S21","DOI":"10.1002\/hep.1840360704","article-title":"Course and outcome of hepatitis C","volume":"36","author":"JH Hoofnagle","year":"2002","journal-title":"Hepatology"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s00535-007-2030-3","article-title":"Molecular biology of hepatitis C virus","volume":"42","author":"T Suzuki","year":"2007","journal-title":"J Gastroenterol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"e1000035","DOI":"10.1371\/journal.ppat.1000035","article-title":"Essential role of domain III of nonstructural protein 5A for hepatitis C virus infectious particle assembly","volume":"4","author":"N Appel","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"12702","DOI":"10.1128\/JVI.01184-09","article-title":"Determinants of the hepatitis C virus nonstructural protein 2 protease domain required for production of infectious virus","volume":"83","author":"TG Dentzer","year":"2009","journal-title":"J Virol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"28546","DOI":"10.1074\/jbc.M803981200","article-title":"Structural and functional characterization of nonstructural protein 2 for its role in hepatitis C virus assembly","volume":"283","author":"V Jirasko","year":"2008","journal-title":"J Biol Chem"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"8374","DOI":"10.1128\/JVI.00690-07","article-title":"Hepatitis C virus p7 and NS2 proteins are essential for production of infectious virus","volume":"81","author":"CT Jones","year":"2007","journal-title":"J Virol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"7624","DOI":"10.1128\/JVI.00724-08","article-title":"NS3 helicase domains involved in infectious intracellular hepatitis C virus particle assembly","volume":"82","author":"Y Ma","year":"2008","journal-title":"J Virol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"7964","DOI":"10.1128\/JVI.00826-08","article-title":"Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles","volume":"82","author":"T Masaki","year":"2008","journal-title":"J Virol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"e1000032","DOI":"10.1371\/journal.ppat.1000032","article-title":"Regulation of hepatitis C virion production via phosphorylation of the NS5A protein","volume":"4","author":"TL Tellinghuisen","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"8379","DOI":"10.1128\/JVI.00891-09","article-title":"Hepatitis C virus NS2 protein contributes to virus particle assembly via opposing epistatic interactions with the E1\u2013E2 glycoprotein and NS3-NS4A enzyme complexes","volume":"83","author":"T Phan","year":"2009","journal-title":"J Virol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1038\/nature04975","article-title":"Structure of the catalytic domain of the hepatitis C virus NS2-3 protease","volume":"442","author":"IC Lorenz","year":"2006","journal-title":"Nature"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1126\/science.285.5424.110","article-title":"Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line","volume":"285","author":"V Lohmann","year":"1999","journal-title":"Science"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1002\/hep.22422","article-title":"Hepatitis C virus JFH-1 strain infection in chimpanzees is associated with low pathogenicity and emergence of an adaptive mutation","volume":"48","author":"T Kato","year":"2008","journal-title":"Hepatology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1073\/pnas.0711044105","article-title":"Development of JFH1-based cell culture systems for hepatitis C virus genotype 4a and evidence for cross-genotype neutralization","volume":"105","author":"TK Scheel","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"1756","DOI":"10.1086\/593021","article-title":"Highly efficient JFH1-based cell-culture system for hepatitis C virus genotype 5a: failure of homologous neutralizing-antibody treatment to control infection","volume":"198","author":"TB Jensen","year":"2008","journal-title":"J Infect Dis"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/JVI.01890-06","article-title":"Compensatory mutations in E1, p7, NS2, and NS3 enhance yields of cell culture-infectious intergenotypic chimeric hepatitis C virus","volume":"81","author":"M Yi","year":"2007","journal-title":"J Virol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"4370","DOI":"10.1073\/pnas.0800422105","article-title":"Advantages of a single-cycle production assay to study cell culture-adaptive mutations of hepatitis C virus","volume":"105","author":"RS Russell","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"e1001278","DOI":"10.1371\/journal.ppat.1001278","article-title":"NS2 protein of hepatitis C virus interacts with structural and non-structural proteins towards virus assembly","volume":"7","author":"CI Popescu","year":"2011","journal-title":"PLoS Pathog"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1128\/JVI.01070-10","article-title":"Hepatitis C virus NS2 protein serves as a scaffold for virus assembly by interacting with both structural and nonstructural proteins","volume":"85","author":"Y Ma","year":"2011","journal-title":"J Virol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1706","DOI":"10.1128\/JVI.02268-10","article-title":"Hepatitis C virus NS2 coordinates virus particle assembly through physical interactions with the E1\u2013E2 glycoprotein and NS3-NS4A enzyme complexes","volume":"85","author":"KA Stapleford","year":"2011","journal-title":"J Virol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e1001233","DOI":"10.1371\/journal.ppat.1001233","article-title":"Structural and functional studies of nonstructural protein 2 of the hepatitis C virus reveal its key role as organizer of virion assembly","volume":"6","author":"V Jirasko","year":"2010","journal-title":"PLoS Pathog"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"e1000403","DOI":"10.1371\/journal.ppat.1000403","article-title":"Trans-complementation of an NS2 defect in a late step in hepatitis C virus (HCV) particle assembly and maturation","volume":"5","author":"M Yi","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"e1002302","DOI":"10.1371\/journal.ppat.1002302","article-title":"Trafficking of hepatitis C virus core protein during virus particle assembly","volume":"7","author":"NA Counihan","year":"2011","journal-title":"PLoS Pathog"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.virusres.2005.10.021","article-title":"Complex formation between hepatitis C virus NS2 and NS3 proteins","volume":"117","author":"K Kiiver","year":"2006","journal-title":"Virus Res"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1006\/viro.1994.1515","article-title":"Complex processing and protein:protein interactions in the E2:NS2 region of HCV","volume":"204","author":"MJ Selby","year":"1994","journal-title":"Virology"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"10340","DOI":"10.1073\/pnas.91.22.10340","article-title":"Split ubiquitin as a sensor of protein interactions in vivo","volume":"91","author":"N Johnsson","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1073\/pnas.83.3.581","article-title":"Purification of microsomal signal peptidase as a complex","volume":"83","author":"EA Evans","year":"1986","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"16460","DOI":"10.1074\/jbc.271.28.16460","article-title":"The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae","volume":"271","author":"H Fang","year":"1996","journal-title":"J Biol Chem"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/nm1268","article-title":"Production of infectious hepatitis C virus in tissue culture from a cloned viral genome","volume":"11","author":"T Wakita","year":"2005","journal-title":"Nat Med"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/nmeth947","article-title":"Direct observation of individual endogenous protein complexes in situ by proximity ligation","volume":"3","author":"O S\u00f6derberg","year":"2006","journal-title":"Nat Methods"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1038\/nmeth1206-969","article-title":"Complementary methods for studies of protein interactions in living cells","volume":"3","author":"TK Kerppola","year":"2006","journal-title":"Nat Methods"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"e51942","DOI":"10.1371\/journal.pone.0051942","article-title":"C911: A bench-level control for sequence specific siRNA off-target effects","volume":"7","author":"E Buehler","year":"2012","journal-title":"PLoS One"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.virol.2012.05.033","article-title":"Trans-complemented hepatitis C virus particles as a versatile tool for study of virus assembly and infection","volume":"432","author":"R Suzuki","year":"2012","journal-title":"Virology"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"13783","DOI":"10.1128\/JVI.01091-07","article-title":"Human apolipoprotein E is required for infectivity and production of hepatitis C virus in cell culture","volume":"81","author":"KS Chang","year":"2007","journal-title":"J Virol"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.virol.2009.08.037","article-title":"Apolipoprotein E on hepatitis C virion facilitates infection through interaction with low-density lipoprotein receptor","volume":"394","author":"DM Owen","year":"2009","journal-title":"Virology"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"5036","DOI":"10.1128\/JVI.01573-06","article-title":"CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection","volume":"81","author":"D Akazawa","year":"2007","journal-title":"J Virol"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"18256","DOI":"10.1074\/jbc.M209732200","article-title":"The hepatitis C virus NS2 protein is an inhibitor of CIDE-B-induced apoptosis","volume":"278","author":"L Erdtmann","year":"2003","journal-title":"J Biol Chem"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1038\/msb.2008.66","article-title":"Hepatitis C virus infection protein network","volume":"4","author":"B de Chassey","year":"2008","journal-title":"Mol Syst Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"5187","DOI":"10.1073\/pnas.95.9.5187","article-title":"A genetic system based on split-ubiquitin for the analysis of interactions between membrane proteins in vivo","volume":"95","author":"I Stagljar","year":"1998","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"3925","DOI":"10.1074\/jbc.271.7.3925","article-title":"Membrane topology of the 12- and the 25-kDa subunits of the mammalian signal peptidase complex","volume":"271","author":"KU Kalies","year":"1996","journal-title":"J Biol Chem"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"29094","DOI":"10.1074\/jbc.271.46.29094","article-title":"Structurally related Spc1p and Spc2p of yeast signal peptidase complex are functionally distinct","volume":"271","author":"C Mullins","year":"1996","journal-title":"J Biol Chem"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"16410","DOI":"10.1073\/pnas.0907439106","article-title":"A genome-wide genetic screen for host factors required for hepatitis C virus propagation","volume":"106","author":"Q Li","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"9294","DOI":"10.1073\/pnas.0503596102","article-title":"Robust hepatitis C virus infection in vitro","volume":"102","author":"J Zhong","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"5824","DOI":"10.1128\/JVI.02397-09","article-title":"Production of infectious hepatitis C virus by using RNA polymerase I-mediated transcription","volume":"84","author":"T Masaki","year":"2010","journal-title":"J Virol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1128\/JVI.79.2.1271-1281.2005","article-title":"Molecular determinants for subcellular localization of hepatitis C virus core protein","volume":"79","author":"R Suzuki","year":"2005","journal-title":"J Virol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1099\/vir.0.80638-0","article-title":"Characterization of the E-138 (Glu\/Lys) mutation in Japanese encephalitis virus by using a stable, full-length, infectious cDNA clone","volume":"86","author":"Z Zhao","year":"2005","journal-title":"J Gen Virol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"37264","DOI":"10.1074\/jbc.M111.259085","article-title":"Role of the endoplasmic reticulum-associated degradation (ERAD) pathway in degradation of hepatitis C virus envelope proteins and production of virus particles","volume":"286","author":"M Saeed","year":"2011","journal-title":"J Biol Chem"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1003589","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T20:51:41Z","timestamp":1588971101000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1003589"}},"subtitle":[],"editor":[{"given":"Aleem","family":"Siddiqui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,8,29]]},"references-count":47,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2013,8,29]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1003589","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,29]]}}}