{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:05:28Z","timestamp":1791291928887,"version":"4.1.0"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2013,12,5]],"date-time":"2013-12-05T00:00:00Z","timestamp":1386201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1003785","type":"journal-article","created":{"date-parts":[[2013,12,5]],"date-time":"2013-12-05T22:02:45Z","timestamp":1386280965000},"page":"e1003785","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":151,"title":["Brucella Induces an Unfolded Protein Response via TcpB That Supports Intracellular Replication in Macrophages"],"prefix":"10.1371","volume":"9","author":[{"given":"Judith A.","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mike","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diogo D.","family":"Magnani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome S.","family":"Harms","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Durward","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girish K.","family":"Radhakrishnan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ping","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary A.","family":"Splitter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2013,12,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1056\/NEJMra050570","article-title":"Brucellosis","volume":"352","author":"G Pappas","year":"2005","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"G Pappas","year":"2006","journal-title":"Lancet Infect Dis"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1146\/annurev-micro-090110-102905","article-title":"Interactions of the human pathogenic Brucella species with their hosts","volume":"65","author":"VL Atluri","year":"2011","journal-title":"Annu Rev of Microbiol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.mib.2004.12.003","article-title":"Brucella lipopolysaccharide acts as a virulence factor","volume":"8","author":"N Lapaque","year":"2005","journal-title":"Curr Opin Microbiol"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1600-065X.2010.00982.x","article-title":"An evolutionary strategy for a stealthy intracellular Brucella pathogen","volume":"240","author":"A Martirosyan","year":"2011","journal-title":"Immunol Rev"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.micpath.2004.04.002","article-title":"Lipid raft microdomains mediate class A scavenger receptor-dependent infection of Brucella abortus","volume":"37","author":"S Kim","year":"2004","journal-title":"Microb Pathog"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1084\/jem.20030088","article-title":"Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum","volume":"198","author":"J Celli","year":"2003","journal-title":"J Exp Med"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"e1000487","DOI":"10.1371\/journal.ppat.1000487","article-title":"The glyceraldehyde-3-phosphate dehydrogenase and the small GTPase Rab 2 are crucial for Brucella replication","volume":"5","author":"E Fugier","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1073\/pnas.0406873102","article-title":"Brucella coopts the small GTPase Sar1 for intracellular replication","volume":"102","author":"J Celli","year":"2005","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"1544","DOI":"10.1073\/pnas.032514299","article-title":"The Brucella suis virB operon is induced intracellularly in macrophages","volume":"99","author":"ML Boschiroli","year":"2002","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1146\/annurev.biochem.73.011303.074134","article-title":"The mammalian unfolded protein response","volume":"74","author":"M Schroder","year":"2005","journal-title":"Annu Rev Biochem"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.molcel.2009.08.021","article-title":"Fine-tuning of the unfolded protein response: Assembling the IRE1alpha interactome","volume":"35","author":"C Hetz","year":"2009","journal-title":"Mol Cell"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"e1000110","DOI":"10.1371\/journal.ppat.1000110","article-title":"RNAi screen of endoplasmic reticulum-associated host factors reveals a role for IRE1alpha in supporting Brucella replication","volume":"4","author":"QM Qin","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"9220","DOI":"10.1128\/MCB.01453-06","article-title":"Autophagy is activated for cell survival after endoplasmic reticulum stress","volume":"26","author":"M Ogata","year":"2006","journal-title":"Mol Cell Biol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"5711","DOI":"10.1128\/IAI.66.12.5711-5724.1998","article-title":"Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes","volume":"66","author":"J Pizarro-Cerda","year":"1998","journal-title":"Infect Immun"},{"key":"ref16","first-page":"249","article-title":"Autophagy favors Brucella melitensis survival in infected macrophages","volume":"17","author":"F Guo","year":"2012","journal-title":"Cell Mol Biol Lett"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.chom.2011.12.002","article-title":"Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle","volume":"11","author":"T Starr","year":"2012","journal-title":"Cell Host Microbe"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1128\/IAI.66.5.2387-2392.1998","article-title":"Virulent Brucella abortus prevents lysosome fusion and is distributed within autophagosome-like compartments","volume":"66","author":"J Pizarro-Cerda","year":"1998","journal-title":"Infect Immun"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e12772","DOI":"10.1371\/journal.pone.0012772","article-title":"Induction of ER stress in macrophages of tuberculosis granulomas","volume":"5","author":"TA Seimon","year":"2010","journal-title":"PLoS One"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1038\/ni.1857","article-title":"TLR activation of the transcription factor XBP1 regulates innate immune responses in macrophages","volume":"11","author":"F Martinon","year":"2010","journal-title":"Nat Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1111\/j.1462-5822.2012.01769.x","article-title":"Activation of the unfolded protein response by Listeria monocytogenes","volume":"14","author":"H Pillich","year":"2012","journal-title":"Cell Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/S1097-2765(00)00108-8","article-title":"Regulated translation initiation controls stress-induced gene expression in mammalian cells","volume":"6","author":"HP Harding","year":"2000","journal-title":"Mol Cell"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"7448","DOI":"10.1128\/MCB.23.21.7448-7459.2003","article-title":"XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response","volume":"23","author":"AH Lee","year":"2003","journal-title":"Mol Cell Biol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.devcel.2007.07.018","article-title":"Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1","volume":"13","author":"K Yamamoto","year":"2007","journal-title":"Dev Cell"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1038\/415092a","article-title":"IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA","volume":"415","author":"M Calfon","year":"2002","journal-title":"Nature"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1038\/ncb1996","article-title":"Adaptive suppression of the ATF4-CHOP branch of the unfolded protein response by toll-like receptor signalling","volume":"11","author":"CW Woo","year":"2009","journal-title":"Nat Cell Biol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.vetimm.2011.05.036","article-title":"Update on the role of innate immune receptors during Brucella abortus infection","volume":"148","author":"SC Oliveira","year":"2011","journal-title":"Vet Immunol Immunopathol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1111\/j.1462-5822.2005.00570.x","article-title":"Temporal analysis of pathogenic events in virulent and avirulent Brucella melitensis infections","volume":"7","author":"G Rajashekara","year":"2005","journal-title":"Cell Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"e21","DOI":"10.1371\/journal.ppat.0040021","article-title":"Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1","volume":"4","author":"SP Salcedo","year":"2008","journal-title":"PLoS Pathog"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"9892","DOI":"10.1074\/jbc.M805458200","article-title":"Brucella TIR Domain-containing Protein Mimics Properties of the Toll-like Receptor Adaptor Protein TIRAP","volume":"284","author":"GK Radhakrishnan","year":"2009","journal-title":"J Biol Chem"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1042\/BJ20110577","article-title":"Modulation of microtubule dynamics by a TIR domain protein from the intracellular pathogen Brucella melitensis","volume":"439","author":"GK Radhakrishnan","year":"2011","journal-title":"The Biochem J"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1979","DOI":"10.1242\/jcs.041962","article-title":"Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells","volume":"122","author":"MJ Wozniak","year":"2009","journal-title":"J Cell Sci"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"3888","DOI":"10.1111\/j.1582-4934.2009.00873.x","article-title":"Blockade of GRP78 sensitizes breast cancer cells to microtubules-interfering agents that induce the unfolded protein response","volume":"13","author":"J Wang","year":"2009","journal-title":"J Cell Mol Med"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1002\/jcb.21730","article-title":"Involvement of endoplasmic reticulum in paclitaxel-induced apoptosis","volume":"104","author":"PC Liao","year":"2008","journal-title":"J Cell Biochem"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1385\/CT:2:2:075","article-title":"Microtubules in cardiac toxicity and disease","volume":"2","author":"DR Webster","year":"2002","journal-title":"Cardiovasc Toxicol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1073\/pnas.0712224105","article-title":"Unfolded protein response and cell death after depletion of brefeldin A-inhibited guanine nucleotide-exchange protein GBF1","volume":"105","author":"C Citterio","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1128\/IAI.00208-12","article-title":"Brucella abortus invasion of osteoblasts inhibits bone formation","volume":"80","author":"R Scian","year":"2012","journal-title":"Infect Immun"},{"key":"ref38","first-page":"20","article-title":"Induction of a th1-type of immune response but not protective immunity by intramuscular DNA immunisation with Brucella abortus GroEL heat-shock gene","volume":"51","author":"S Leclerq","year":"2002","journal-title":"J MedMicrobiol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"4255","DOI":"10.1128\/IAI.68.7.4255-4263.2000","article-title":"Intracellular trafficking of Brucella abortus in J774 macrophages","volume":"68","author":"GN Arenas","year":"2000","journal-title":"Infect Immun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"e00418","DOI":"10.1128\/mBio.00418-12","article-title":"Sensing of Bacterial Type IV Secretion via the Unfolded Protein Response","volume":"4","author":"MF de Jong","year":"2013","journal-title":"MBio"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1016\/S1097-2765(03)00105-9","article-title":"An integrated stress response regulates amino acid metabolism and resistance to oxidative stress","volume":"11","author":"HP Harding","year":"2003","journal-title":"Mol Cell"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1091\/mbc.E03-11-0799","article-title":"Diverse and specific gene expression responses to stresses in cultured human cells","volume":"15","author":"JI Murray","year":"2004","journal-title":"Mol Biol Cell"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1093\/intimm\/dxh148","article-title":"Interaction of endotoxins with Toll-like receptor 4 correlates with their endotoxic potential and may explain the proinflammatory effect of Brucella spp. LPS","volume":"16","author":"AI Duenas","year":"2004","journal-title":"Int Immunol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"6243","DOI":"10.1128\/JB.00520-08","article-title":"Genomic island 2 of Brucella melitensis is a major virulence determinant: functional analyses of genomic islands","volume":"190","author":"G Rajashekara","year":"2008","journal-title":"J Bacteriol"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/path.1798","article-title":"Distinct endoplasmic reticulum stress responses are triggered during human liver transplantation","volume":"207","author":"A Emadali","year":"2005","journal-title":"J Pathol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"17158","DOI":"10.1074\/jbc.M312144200","article-title":"Hepatitis C virus suppresses the IRE1-XBP1 pathway of the unfolded protein response","volume":"279","author":"KD Tardif","year":"2004","journal-title":"J Biol Chem"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"e23512","DOI":"10.1371\/journal.pone.0023512","article-title":"An atypical unfolded protein response in heat shocked cells","volume":"6","author":"L Heldens","year":"2011","journal-title":"PLoS One"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"14226","DOI":"10.1074\/jbc.M111.222703","article-title":"Dengue virus modulates the unfolded protein response in a time-dependent manner","volume":"286","author":"J Pena","year":"2011","journal-title":"J Biol Chem"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1463-1326.2010.01282.x","article-title":"Restoring endoplasmic reticulum function by chemical chaperones: an emerging therapeutic approach for metabolic diseases","volume":"12 Suppl 2","author":"F Engin","year":"2010","journal-title":"Diabetes Obes Metab"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.2337\/db10-0308","article-title":"Tauroursodeoxycholic Acid may improve liver and muscle but not adipose tissue insulin sensitivity in obese men and women","volume":"59","author":"M Kars","year":"2010","journal-title":"Diabetes"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1002\/eji.200737882","article-title":"Endoplasmic reticulum stress and the unfolded protein response are linked to synergistic IFN-beta induction via X-box binding protein 1","volume":"38","author":"JA Smith","year":"2008","journal-title":"Eur J Immunol"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1038\/icb.2011.112","article-title":"The interplay between endoplasmic reticulum stress and inflammation","volume":"90","author":"SZ Hasnain","year":"2012","journal-title":"Immunol Cell Biol"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1083\/jcb.201110131","article-title":"The impact of the unfolded protein response on human disease","volume":"197","author":"S Wang","year":"2012","journal-title":"J Cell Biol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1111\/j.1462-5822.2011.01594.x","article-title":"Immunologic considerations for generating memory CD8 T cells through vaccination","volume":"13","author":"NS Butler","year":"2011","journal-title":"Cell Microbiol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1038\/nri1775","article-title":"Pathogen-endoplasmic-reticulum interactions: in through the out door","volume":"6","author":"CR Roy","year":"2006","journal-title":"Nat Rev Immunol"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1574-6968.2008.01358.x","article-title":"Activity of native vs. synthetic promoters in Brucella","volume":"288","author":"MN Seleem","year":"2008","journal-title":"FEMS Microbiol Lett"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1093\/cvr\/cvs119","article-title":"Ablation of junctin or triadin is associated with increased cardiac injury following ischaemia\/reperfusion","volume":"94","author":"WF Cai","year":"2012","journal-title":"Cardiovasc Res"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1003785","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T14:08:02Z","timestamp":1597154882000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1003785"}},"subtitle":[],"editor":[{"given":"Craig R.","family":"Roy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,12,5]]},"references-count":57,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2013,12,5]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1003785","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,5]]}}}