{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:34:41Z","timestamp":1790793281437,"version":"4.1.0"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T00:00:00Z","timestamp":1395878400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1004046","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T17:11:10Z","timestamp":1395940270000},"page":"e1004046","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":76,"title":["Heterogeneity and Breadth of Host Antibody Response to KSHV Infection Demonstrated by Systematic Analysis of the KSHV Proteome"],"prefix":"10.1371","volume":"10","author":[{"given":"Nazzarena","family":"Labo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendell","family":"Miley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vickie","family":"Marshall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Gillette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Esposito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Bess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Turano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Uldrick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark N.","family":"Polizzotto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen M.","family":"Wyvill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Bagni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Yarchoan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Whitby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2014,3,27]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1126\/science.7997879","article-title":"Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi&apos;s sarcoma","volume":"266","author":"Y Chang","year":"1994","journal-title":"Science"},{"key":"ref2","first-page":"53","article-title":"Kaposi&apos;s sarcoma-associated herpesvirus in non-AIDS related lymphomas occurring in body cavities","volume":"149","author":"E Cesarman","year":"1996","journal-title":"Am J Pathol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1182\/blood.V86.4.1276.bloodjournal8641276","article-title":"Kaposi&apos;s sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman&apos;s disease","volume":"86","author":"J Soulier","year":"1995","journal-title":"Blood"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1086\/598523","article-title":"Geographic Variation in the Prevalence of Kaposi Sarcoma-Associated Herpesvirus and Risk Factors for Transmission","volume":"199","author":"S de Sanjose","year":"2009","journal-title":"J Infect Dis"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1097\/QAD.0b013e3282f46582","article-title":"No evidence of sexual transmission of Kaposi&apos;s sarcoma herpes virus in a heterosexual South African population","volume":"22","author":"BI Malope","year":"2008","journal-title":"Aids"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1750-9378-5-14","article-title":"Kaposi&apos;s Sarcoma Associated-Herpes Virus (KSHV) Seroprevalence in Pregnant Women in South Africa","volume":"5","author":"BI Malope-Kgokong","year":"2010","journal-title":"Infect Agent Cancer"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1186\/1750-9378-6-15","article-title":"Parasite infection is associated with Kaposi&apos;s sarcoma associated herpesvirus (KSHV) in Ugandan women","volume":"6","author":"K Wakeham","year":"2011","journal-title":"Infect Agent Cancer"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1023\/A:1015678312153","article-title":"A seroprevalence study of human herpesvirus type 8 (HHV8) in eastern and Central Africa and in the Mediterranean area","volume":"17","author":"D Serraino","year":"2001","journal-title":"Eur J Epidemiol"},{"key":"ref9","first-page":"588","article-title":"Kaposi&apos;s sarcoma herpes virus and Kaposi&apos;s sarcoma in the elderly populations of 3 Mediterranean islands","volume":"91","author":"F Vitale","year":"2001","journal-title":"Int J Cancer"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1099\/vir.0.19465-0","article-title":"Genotypic characterization of Kaposi&apos;s sarcoma-associated herpesvirus in asymptomatic infected subjects from isolated populations","volume":"85","author":"D Whitby","year":"2004","journal-title":"J Gen Virol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1086\/520549","article-title":"Human herpesvirus-8 infection and oral shedding in Amerindian and non-Amerindian populations in the Brazilian Amazon region","volume":"196","author":"VA de Souza","year":"2007","journal-title":"J Infect Dis"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1002\/ijc.22281","article-title":"Risk factors for human Herpesvirus 8 infection and AIDS-associated Kaposi&apos;s sarcoma among men who have sex with men in a European multicentre study","volume":"120","author":"E Martro","year":"2007","journal-title":"Int J Cancer"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1086\/339605","article-title":"Correlates of prevalent and incident Kaposi&apos;s sarcoma-associated herpesvirus infection in men who have sex with men","volume":"185","author":"C Casper","year":"2002","journal-title":"J Infect Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1086\/314926","article-title":"Serologic evidence of human herpesvirus 8 transmission by homosexual but not heterosexual sex","volume":"180","author":"NA Smith","year":"1999","journal-title":"J Infect Dis"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1128\/JCM.01264-06","article-title":"Comparative study of Kaposi&apos;s sarcoma-associated herpesvirus serological assays using clinically and serologically defined reference standards and latent class analysis","volume":"45","author":"MC Nascimento","year":"2007","journal-title":"J Clin Microbiol"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1128\/CDLI.7.3.427-435.2000","article-title":"New immunofluorescence assays for detection of Human herpesvirus 8-specific antibodies","volume":"7","author":"N Inoue","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1128\/JCM.40.02.325-329.2002","article-title":"Highly sensitive assay for human herpesvirus 8 antibodies that uses a multiple antigenic peptide derived from open reading frame K8.1","volume":"40","author":"LL Lam","year":"2002","journal-title":"J Clin Microbiol"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.jim.2010.02.015","article-title":"Detection of antibodies to Kaposi&apos;s sarcoma-associated herpesvirus: a new approach using K8.1 ELISA and a newly developed recombinant LANA ELISA","volume":"356","author":"GL Mbisa","year":"2010","journal-title":"J Immunol Methods"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1128\/CVI.00474-08","article-title":"Four-antigen mixture containing v-cyclin for serological screening of human herpesvirus 8 infection","volume":"16","author":"PD Burbelo","year":"2009","journal-title":"Clin Vaccine Immunol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1919","DOI":"10.1086\/652869","article-title":"Distinct profiles of antibodies to Kaposi sarcoma-associated herpesvirus antigens in patients with Kaposi sarcoma, multicentric Castleman disease, and primary effusion lymphoma","volume":"201","author":"PD Burbelo","year":"2010","journal-title":"The Journal of infectious diseases"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1086\/427194","article-title":"Persistence of Kaposi sarcoma-associated herpesvirus (KSHV)-infected cells in KSHV\/HIV-1-coinfected subjects without KSHV-associated diseases","volume":"191","author":"TB Campbell","year":"2005","journal-title":"J Infect Dis"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1099\/vir.0.027847-0","article-title":"Intrafamiliar transmission of Kaposi&apos;s sarcoma-associated herpesvirus and seronegative infection in family members of classic Kaposi&apos;s sarcoma patients","volume":"92","author":"R Mancuso","year":"2011","journal-title":"The Journal of general virology"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1128\/JCM.38.4.1404-1408.2000","article-title":"Quantitative analysis of human herpesvirus 8 viral load using a real- time PCR assay","volume":"38","author":"F Lallemand","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"2174","DOI":"10.1128\/JCM.38.6.2174-2180.2000","article-title":"Comparison of serologic assays and PCR for diagnosis of human herpesvirus 8 infection","volume":"38","author":"TJ Spira","year":"2000","journal-title":"J Clin Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1086\/345866","article-title":"Antibody reactivity to latent and lytic antigens to human herpesvirus-8 in longitudinally followed homosexual men","volume":"187","author":"RJ Biggar","year":"2003","journal-title":"J Infect Dis"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1111\/j.0041-1132.2005.04334.x","article-title":"Possible transmission of human herpesvirus-8 by blood transfusion in a historical United States cohort","volume":"45","author":"SC Dollard","year":"2005","journal-title":"Transfusion"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/jmv.1031","article-title":"Longitudinal sero-reactivity to human herpesvirus 8 (KSHV) in the Swiss HIV Cohort 4.7 years before KS","volume":"64","author":"EB Quinlivan","year":"2001","journal-title":"J Med Virol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1093\/aje\/kwn125","article-title":"Early childhood infection by human herpesvirus 8 in Zambia and the role of human immunodeficiency virus type 1 coinfection in a highly endemic area","volume":"168","author":"V Minhas","year":"2008","journal-title":"Am J Epidemiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1093\/infdis\/jir645","article-title":"Comparison of humoral immune responses to Epstein-Barr virus and Kaposi&apos;s sarcoma-associated herpesvirus using a viral proteome microarray","volume":"204","author":"D Zheng","year":"2011","journal-title":"The Journal of infectious diseases"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1046\/j.1537-2995.2003.00490.x","article-title":"Multicenter comparison of serologic assays and estimation of human herpesvirus 8 seroprevalence among US blood donors","volume":"43","author":"PE Pellett","year":"2003","journal-title":"Transfusion"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"14862","DOI":"10.1073\/pnas.93.25.14862","article-title":"Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8)","volume":"93","author":"JJ Russo","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"785","DOI":"10.2144\/000113238","article-title":"A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line","volume":"47","author":"R Hopkins","year":"2009","journal-title":"BioTechniques"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1086\/654798","article-title":"An interleukin-6-related systemic inflammatory syndrome in patients co-infected with Kaposi sarcoma-associated herpesvirus and HIV but without Multicentric Castleman disease","volume":"51","author":"TS Uldrick","year":"2010","journal-title":"Clin Infect Dis"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1006\/viro.1998.9316","article-title":"Human herpesvirus-8 ORF K8.1 gene encodes immunogenic glycoproteins generated by spliced transcripts","volume":"249","author":"B Chandran","year":"1998","journal-title":"Virology"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1002\/jmv.21485","article-title":"Serological evidence for long-term Epstein-Barr virus reactivation in children living in a holoendemic malaria region of Kenya","volume":"81","author":"E Piriou","year":"2009","journal-title":"J Med Virol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/JCM.44.2.504-512.2006","article-title":"Bead-based multiplex genotyping of human papillomaviruses","volume":"44","author":"M Schmitt","year":"2006","journal-title":"Journal of clinical microbiology"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1016\/j.acra.2012.09.006","article-title":"Applications of ROC analysis in medical research: recent developments and future directions","volume":"19","author":"D Alemayehu","year":"2012","journal-title":"Acad Radiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1084\/jem.20050882","article-title":"Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects","volume":"202","author":"AW Sylwester","year":"2005","journal-title":"J Exp Med"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1172\/JCI60556","article-title":"Cross-presentation and genome-wide screening reveal candidate T cells antigens for a herpes simplex virus type 1 vaccine","volume":"122","author":"L Jing","year":"2012","journal-title":"The Journal of clinical investigation"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"10920","DOI":"10.1128\/JVI.74.23.10920-10929.2000","article-title":"Characterization of human herpesvirus 8 ORF59 protein (PF-8) and mapping of the processivity and viral DNA polymerase-interacting domains","volume":"74","author":"SR Chan","year":"2000","journal-title":"J Virol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1002\/(SICI)1096-9071(199911)59:3<346::AID-JMV15>3.0.CO;2-4","article-title":"Expression and antigenicity of human herpesvirus 8 encoded ORF59 protein in AIDS-associated Kaposi&apos;s sarcoma","volume":"59","author":"H Katano","year":"1999","journal-title":"J Med Virol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"e1000843","DOI":"10.1371\/journal.ppat.1000843","article-title":"The RING-CH Ligase K5 Antagonizes Restriction of KSHV and HIV-1 Particle Release by Mediating Ubiquitin-Dependent Endosomal Degradation of Tetherin","volume":"6","author":"C Pardieu","year":"2010","journal-title":"PLoS pathogens"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"5300","DOI":"10.1128\/JVI.74.11.5300-5309.2000","article-title":"Downregulation of major histocompatibility complex class I molecules by Kaposi&apos;s sarcoma-associated herpesvirus K3 and K5 proteins","volume":"74","author":"S Ishido","year":"2000","journal-title":"J Virol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/S1074-7613(00)00036-4","article-title":"Inhibition of natural killer cell-mediated cytotoxicity by Kaposi&apos;s sarcoma-associated herpesvirus K5 protein","volume":"13","author":"S Ishido","year":"2000","journal-title":"Immunity"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1172\/JCI12432","article-title":"A viral protein that selectively downregulates ICAM-1 and B7-2 and modulates T cell costimulation","volume":"107","author":"L Coscoy","year":"2001","journal-title":"J Clin Invest"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e58056","DOI":"10.1371\/journal.pone.0058056","article-title":"Kaposi&apos;s Sarcoma-Associated Herpesvirus K3 and K5 Proteins Down Regulate Both DC-SIGN and DC-SIGNR","volume":"8","author":"SM Lang","year":"2013","journal-title":"PloS one"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"3250","DOI":"10.4049\/jimmunol.0900722","article-title":"Detailed characterization of T cell responses to herpes simplex virus-2 in immune seronegative persons","volume":"184","author":"CM Posavad","year":"2010","journal-title":"Journal of immunology"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.ymeth.2005.11.008","article-title":"Solid-phase and bead-based cytokine immunoassay: a comparison","volume":"38","author":"W de Jager","year":"2006","journal-title":"Methods"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"1819","DOI":"10.1097\/00002030-200409030-00011","article-title":"Repeated measures study of human herpesvirus 8 (HHV-8) DNA and antibodies in men seropositive for both HHV-8 and HIV","volume":"18","author":"AS Laney","year":"2004","journal-title":"Aids"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"1863","DOI":"10.1056\/NEJM199906173402403","article-title":"Antibodies against human herpesvirus 8 in black South African patients with cancer","volume":"340","author":"F Sitas","year":"1999","journal-title":"N Engl J Med"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1097\/00002030-200301240-00012","article-title":"Risk factors for Kaposi&apos;s sarcoma in men seropositive for both human herpesvirus 8 and human immunodeficiency virus","volume":"17","author":"MJ Cannon","year":"2003","journal-title":"Aids"}],"container-title":["PLoS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1004046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T18:47:49Z","timestamp":1597603669000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1004046"}},"subtitle":[],"editor":[{"given":"Dirk P.","family":"Dittmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,3,27]]},"references-count":51,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2014,3,27]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1004046","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,27]]}}}