{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:03:19Z","timestamp":1787022199322,"version":"build-2736575974"},"reference-count":46,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T00:00:00Z","timestamp":1431043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1004887","type":"journal-article","created":{"date-parts":[[2015,5,8]],"date-time":"2015-05-08T14:11:18Z","timestamp":1431094278000},"page":"e1004887","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":77,"title":["The NLRP3 Inflammasome Is a Pathogen Sensor for Invasive Entamoeba histolytica via Activation of \u03b15\u03b21 Integrin at the Macrophage-Amebae Intercellular Junction"],"prefix":"10.1371","volume":"11","author":[{"given":"Leanne","family":"Mortimer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"France","family":"Moreau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Cornick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"Chadee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,5,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/S0140-6736(03)12830-9","article-title":"Ameobiasis","volume":"361","author":"S.L. Stanley","year":"2003","journal-title":"Lancet"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.exppara.2010.03.005","article-title":"The Immunopathogenesis of <italic>Entamoeba histolytica<\/italic>","volume":"126","author":"L. Mortimer","year":"2010","journal-title":"Exp. Parasitol"},{"key":"ref3","first-page":"229","article-title":"The histopathology of acute intestinal amebiasis. A rectal biopsy study","volume":"60","author":"K. Prathap","year":"1970","journal-title":"Am. J. Pathol."},{"key":"ref4","doi-asserted-by":"crossref","first-page":"283","DOI":"10.4269\/ajtmh.1985.34.283","article-title":"<italic>Entamoeba histolytica<\/italic>: early progressive pathology in the cecum of the gerbil (<italic>Meriones unguiculatus<\/italic>)","volume":"34","author":"K. Chadee","year":"1985","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref5","doi-asserted-by":"crossref","first-page":"3999","DOI":"10.4049\/jimmunol.148.12.3999","article-title":"Macrophage cytotoxicity against <italic>Entamoeba histolytica<\/italic> trophozoites is mediated by nitric oxide from L-arginine","volume":"148","author":"J.Y Lin","year":"1992","journal-title":"J. Immunol."},{"key":"ref6","doi-asserted-by":"crossref","first-page":"12175","DOI":"10.1073\/pnas.92.26.12175","article-title":"Identification of the galactose-adherence lectin epitopes of <italic>Entamoeba histolytica<\/italic> that stimulate tumor necrosis factor-alpha production in macrophages","volume":"92","author":"R. Seguin","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"ref7","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1038\/nri3244","article-title":"Information processing during phagocytosis","volume":"12","author":"D.M. Underhill","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"ref8","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1038\/mi.2013.100","article-title":"Gal-lectin-dependent contact activates the inflammasome by invasive <italic>Entamoeba histolytica<\/italic>","volume":"4","author":"L. Mortimer","year":"2014","journal-title":"Mucosal Immunol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1038\/nri3452","article-title":"Activation and regulation of the inflammasomes","volume":"13","author":"E. Latz","year":"2013","journal-title":"Nat. Rev. Immunol."},{"key":"ref10","doi-asserted-by":"crossref","first-page":"13273","DOI":"10.1074\/jbc.271.22.13273","article-title":"Activation of the native 45 kDa precursor form of interlekuin-1 converting enzyme","volume":"271","author":"T. Yamin","year":"1996","journal-title":"J. Biol. Chem."},{"key":"ref11","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/ni.1631","article-title":"Silica crystals and aluminum salts mediate NALP-3 inflammasome activation via phagosomal destabilization","volume":"9","author":"V. Hornung","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref12","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/ni.1636","article-title":"The NALP3 inflammasome is involved in the innate immune response to amyloid-b","volume":"9","author":"A. Halle","year":"2008","journal-title":"Nat. Immunol."},{"key":"ref13","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1038\/nature08938","article-title":"NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals","volume":"464","author":"P. Duewell","year":"2010","journal-title":"Nature"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1038\/ni.2550","article-title":"Microtubule-driven spatial arrangement of mitochondria promotes activation of the NLRP3 inflammasome","volume":"14","author":"T. Misawa","year":"2013","journal-title":"Nat. Immunol."},{"key":"ref15","doi-asserted-by":"crossref","first-page":"35497","DOI":"10.1074\/jbc.M109.066035","article-title":"<italic>Entamoeba histolytica<\/italic> cysteine proteinase 5 binds integrin on colonic cells and stimulates NFkappaB-mediated pro-inflammatory responses","volume":"285","author":"Y. Hou","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref16","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1038\/nri1646","article-title":"Intracellular signaling controlling integrin activation in lymphocytes","volume":"5","author":"T. Kinashi","year":"2005","journal-title":"Nat. Rev. Immunol."},{"key":"ref17","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1146\/annurev.cellbio.12.1.697","article-title":"RGD and other recognition sequences for integrins","volume":"12","author":"E. Ruoslahti","year":"1996","journal-title":"Annu. Rev. Cell. Dev. Biol."},{"key":"ref18","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1038\/nrm3599","article-title":"Integrin inactivators: balancing cellular functions <italic>in vitro<\/italic> and <italic>in vivo<\/italic>","volume":"14","author":"D. Bouvard","year":"2013","journal-title":"Nat. Rev. Mol. Cell. Bio."},{"key":"ref19","doi-asserted-by":"crossref","first-page":"6470","DOI":"10.1073\/pnas.83.17.6470","article-title":"Phosphorylation of the fibronectin receptor complex in cells transformed by oncogenes that encode tyrosine kinases","volume":"83","author":"R. Hirst","year":"1986","journal-title":"Proc. Nat. Acad. Sci. USA."},{"key":"ref20","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.virol.2010.07.012","article-title":"Characterization of entry and infection of monocytic THP-1 cells by Kaposi&apos;s sarcoma associated herpesvirus (KSHV): Role of heparan sulfate, DC-SIGN, integrins and signaling","volume":"406","author":"N. Kerur","year":"2010","journal-title":"Virology"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"e64187","DOI":"10.1371\/journal.pone.0064187","article-title":"Elongated membrane tethers, individually anchored by high affinity alpha(4)beta(1)\/VCAM-1 complexes, are the quantal units of monocyte arrests","volume":"8","author":"C. Chu","year":"2013","journal-title":"PLoS One"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"16555","DOI":"10.1074\/jbc.M009068200","article-title":"Distinct signaling pathways for MCP-1-dependent integrin activation and chemotaxis","volume":"276","author":"N. Ashida","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref23","doi-asserted-by":"crossref","first-page":"6347","DOI":"10.1093\/emboj\/20.22.6347","article-title":"Proteomic and functional evidence for a P2X7 receptor signalling complex","volume":"20","author":"M. Kim","year":"2001","journal-title":"EMBO J"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1042\/bj20020439","article-title":"The evidence for two opposite, ATP-generating and ATP-consuming, extracellular pathways on endothelial and lymphoid cells","volume":"367","author":"G.G. Yegutkin","year":"2002","journal-title":"Biochem. J."},{"key":"ref25","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1016\/j.febslet.2014.02.004","article-title":"Differentiating connexin hemichannels and pannexin channels in cellular ATP release","volume":"588","author":"A.W. Lohman","year":"2014","journal-title":"FEBS Lett"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1016\/j.febslet.2014.03.009","article-title":"The role of pannexin1 in the induction and resolution of inflammation","volume":"588","author":"S.E. Adamson","year":"2014","journal-title":"FEBS Lett"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"5071","DOI":"10.1038\/sj.emboj.7601378","article-title":"Pannexin-1 mediates large pore formation and interleukin-1\u03b2 release by the ATP-gated P2X7 receptor","volume":"25","author":"P. Pelegrin","year":"2006","journal-title":"EMBO J"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"6553","DOI":"10.4049\/jimmunol.1100478","article-title":"Pannexin-1 is required for ATP release during apoptosis but not for inflammasome activation","volume":"186","author":"Y. Qu","year":"2011","journal-title":"J. Immunol."},{"key":"ref29","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s13238-013-2114-1","article-title":"Pannexin-1 influences peritoneal cavity cell population but is not involved in NLRP3 inflammasome activation","volume":"4","author":"H. Wang","year":"2013","journal-title":"Protein Cell"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1038\/nature09413","article-title":"Pannexin 1 channelsmediate \u2018find-me\u2019 signal release and membrane permeability during apoptosis","volume":"467","author":"F. Chekeni","year":"2010","journal-title":"Nature"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1128\/IAI.71.2.964-972.2003","article-title":"Apoptotic killing and phagocytosis of host cells by the parasite <italic>Entamoeba histolytica<\/italic>","volume":"71","author":"C.D Huston","year":"2003","journal-title":"Infect Imm"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"12579","DOI":"10.1523\/JNEUROSCI.1267-12.2012","article-title":"Anoxia-induced NMDA receptor activation opens pannexin channels via Src family kinases","volume":"36","author":"N.L Weilinger","year":"2012","journal-title":"J. Neurosci"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"C752","DOI":"10.1152\/ajpcell.00228.2008","article-title":"P2X7 receptor-Pannexin1 complex: pharmacology and signaling","volume":"295","author":"R. Iglesias","year":"2008","journal-title":"Am. J. Physiol. Cell Physiol"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1006\/prep.2001.1548","article-title":"Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite <italic>Entamoeba histolytica<\/italic>","volume":"24","author":"A. Hellberg","year":"2002","journal-title":"Protein Expr. Purif."},{"key":"ref35","doi-asserted-by":"crossref","first-page":"38260","DOI":"10.1074\/jbc.M405308200","article-title":"Resistance of <italic>Entamoeba histolytica<\/italic> to the cysteine proteinase inhibitor E64 is associated with secretion of pro-enzymes and reduced pathogenicity","volume":"279","author":"N. Nowak","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref36","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1016\/j.ajpath.2012.11.035","article-title":"<italic>Entamoeba histolytica<\/italic> exacerbates epithelial tight junction permeability and proinflammatory responses in Muc2(-\/-) mice","volume":"182","author":"V. Kissoon-Singh","year":"2013","journal-title":"Am. J. Pathol."},{"key":"ref37","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.molbiopara.2006.04.009","article-title":"Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of <italic>Entamoeba histolytica<\/italic>","volume":"149","author":"M. Tillack","year":"2006","journal-title":"Mol Biochem Parasitol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1128\/IAI.67.1.421-422.1999","article-title":"Antisense inhibition of expression of cysteine proteinases affects <italic>Entamoeba histolytica<\/italic>-induced formation of liver abscess in hamsters","volume":"67","author":"S. Ankri","year":"1999","journal-title":"Infect. Immun."},{"key":"ref39","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1046\/j.1365-2958.2000.02037.x","article-title":"<italic>Entamoeba histolytica<\/italic> cysteine proteinases with interleukin-1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis","volume":"37","author":"Z. Zhang","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref40","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1146\/annurev-biophys-042910-155238","article-title":"Receptor signaling clusters at immune synapses","volume":"41","author":"M.L. Dustin","year":"2012","journal-title":"Annu. Rev. Biophys."},{"key":"ref41","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1038\/nature10071","article-title":"Activation of the innate immune receptor Dectin-1 upon formation of a \u2018phagocytic synapse.\u2019","volume":"472","author":"H. Goodridge","year":"2011","journal-title":"Nature"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1126\/scisignal.1160583","article-title":"Purinergic control of T cell activation by ATP released through Pannexin-1 Hemichannels","volume":"1","author":"U. Schenk","year":"2008","journal-title":"Sci. Signal."},{"key":"ref43","doi-asserted-by":"crossref","first-page":"3475","DOI":"10.1182\/blood-2010-04-277707","article-title":"Pannexin-1 hemichannel-mediated ATP release together with P2X1 and P2X4 receptors regulate T-cell activation at the immune synapse","volume":"116","author":"T. Woehrle","year":"2010","journal-title":"Blood"},{"key":"ref44","first-page":"1","article-title":"Integrin a5b1 activates the NLRP3 inflammasome by direct interaction with a bacterial surface protein","volume":"36","author":"K. Jun","year":"2012","journal-title":"Immunity"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/0035-9203(78)90144-X","article-title":"A new medium for the axenic cultivation of <italic>Entamoeba histolytica<\/italic>","volume":"72","author":"L.S. Diamond","year":"1978","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref46","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1128\/IAI.57.6.1750-1756.1989","article-title":"Cytokine activation of murine macrophages for <italic>in vitro<\/italic> killing of <italic>Entamoeba histolytica<\/italic> trophozoites","volume":"57","author":"M. Denis","year":"1989","journal-title":"Infect. Immun."}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1004887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,10]],"date-time":"2023-08-10T03:11:10Z","timestamp":1691637070000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1004887"}},"subtitle":[],"editor":[{"given":"William A.","family":"Petri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,5,8]]},"references-count":46,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2015,5,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1004887","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,8]]}}}