{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T14:08:26Z","timestamp":1791986906676,"version":"4.4.0"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2015,9,17]],"date-time":"2015-09-17T00:00:00Z","timestamp":1442448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1005142","type":"journal-article","created":{"date-parts":[[2015,9,18]],"date-time":"2015-09-18T01:51:50Z","timestamp":1442541110000},"page":"e1005142","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":511,"title":["The Depsipeptide Romidepsin Reverses HIV-1 Latency In Vivo"],"prefix":"10.1371","volume":"11","author":[{"given":"Ole S.","family":"S\u00f8gaard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette E.","family":"Graversen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Leth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke","family":"Olesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel R.","family":"Brinkmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara K.","family":"Nissen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne Sofie","family":"Kjaer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariane H.","family":"Schleimann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W.","family":"Denton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Hey-Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kersten K.","family":"Koelsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Pantaleo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Krogsgaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maja","family":"Sommerfelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Remi","family":"Fromentin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Chomont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Rasmussen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"\u00d8stergaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Tolstrup","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2015,9,17]]},"reference":[{"issue":"9117","key":"ref1","doi-asserted-by":"crossref","first-page":"1682","DOI":"10.1016\/S0140-6736(97)10291-4","article-title":"Long-lasting recovery in CD4 T-cell function and viral-load reduction after highly active antiretroviral therapy in advanced HIV-1 disease","volume":"351","author":"TS Li","year":"1998","journal-title":"Lancet"},{"issue":"5","key":"ref2","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1002\/j.1460-2075.1990.tb08274.x","article-title":"HIV-1 replication is controlled at the level of T cell activation and proviral integration","volume":"9","author":"M Stevenson","year":"1990","journal-title":"Embo J"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"a006916","DOI":"10.1101\/cshperspect.a006916","article-title":"Transcriptional and Posttranscriptional Regulation of HIV-1 Gene Expression","volume":"2","author":"J Karn","year":"2012","journal-title":"Cold Spring Harb Perspect Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1146\/annurev.immunol.18.1.665","article-title":"Reservoirs for HIV-1: mechanisms for viral persistence in the presence of antiviral immune responses and antiretroviral therapy","volume":"18","author":"T Pierson","year":"2000","journal-title":"Annu Rev Immunol"},{"issue":"5341","key":"ref5","doi-asserted-by":"crossref","first-page":"1295","DOI":"10.1126\/science.278.5341.1295","article-title":"Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy","volume":"278","author":"D Finzi","year":"1997","journal-title":"Science"},{"issue":"6629","key":"ref6","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/387183a0","article-title":"Quantification of latent tissue reservoirs and total body viral load in HIV-1 infection","volume":"387","author":"TW Chun","year":"1997","journal-title":"Nature"},{"issue":"7512","key":"ref7","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/nature13594","article-title":"Rapid seeding of the viral reservoir prior to SIV viraemia in rhesus monkeys","volume":"512","author":"JB Whitney","year":"2014","journal-title":"Nature"},{"issue":"6196","key":"ref8","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1126\/science.1256304","article-title":"HIV latency. Proliferation of cells with HIV integrated into cancer genes contributes to persistent infection","volume":"345","author":"TA Wagner","year":"2014","journal-title":"Science"},{"issue":"6193","key":"ref9","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1126\/science.1254194","article-title":"HIV latency. Specific HIV integration sites are linked to clonal expansion and persistence of infected cells","volume":"345","author":"F Maldarelli","year":"2014","journal-title":"Science"},{"issue":"3","key":"ref10","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.immuni.2012.08.010","article-title":"Redefining the viral reservoirs that prevent HIV-1 eradication","volume":"37","author":"E Eisele","year":"2012","journal-title":"Immunity"},{"issue":"7408","key":"ref11","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/487439a","article-title":"HIV: Shock and kill","volume":"487","author":"SG Deeks","year":"2012","journal-title":"Nature"},{"issue":"5","key":"ref12","doi-asserted-by":"crossref","first-page":"1901","DOI":"10.1172\/JCI80142","article-title":"Ex vivo analysis identifies effective HIV-1 latency-reversing drug combinations","volume":"125","author":"GM Laird","year":"2015","journal-title":"J Clin Invest"},{"issue":"4","key":"ref13","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/nm.3489","article-title":"New ex vivo approaches distinguish effective and ineffective single agents for reversing HIV-1 latency in vivo","volume":"20","author":"CK Bullen","year":"2014","journal-title":"Nat Med"},{"issue":"17","key":"ref14","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1097\/00002030-199912030-00012","article-title":"Immuno-activation with anti-CD3 and recombinant human IL-2 in HIV-1-infected patients on potent antiretroviral therapy","volume":"13","author":"JM Prins","year":"1999","journal-title":"Aids"},{"issue":"5","key":"ref15","doi-asserted-by":"crossref","DOI":"10.4161\/hv.23800","article-title":"Comparison of HDAC inhibitors in clinical development: Effect on HIV production in latently infected cells and T-cell activation","volume":"9","author":"TA Rasmussen","year":"2013","journal-title":"Hum Vaccin Immunother"},{"issue":"1","key":"ref16","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1182\/blood-2008-07-168393","article-title":"Induction of HIV-1 latency and reactivation in primary memory CD4+ T cells","volume":"113","author":"A Bosque","year":"2009","journal-title":"Blood"},{"issue":"5919","key":"ref17","doi-asserted-by":"crossref","first-page":"1304","DOI":"10.1126\/science.1165706","article-title":"The challenge of finding a cure for HIV infection","volume":"323","author":"DD Richman","year":"2009","journal-title":"Science"},{"issue":"5","key":"ref18","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1002\/j.1460-2075.1996.tb00449.x","article-title":"Transcriptional activation and chromatin remodeling of the HIV-1 promoter in response to histone acetylation","volume":"15","author":"C Van Lint","year":"1996","journal-title":"Embo J"},{"issue":"8","key":"ref19","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1097\/00002030-200405210-00003","article-title":"Coaxing HIV-1 from resting CD4 T cells: histone deacetylase inhibition allows latent viral expression","volume":"18","author":"L Ylisastigui","year":"2004","journal-title":"Aids"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/icb.2011.95","article-title":"HDAC inhibitors in HIV","volume":"90","author":"F Wightman","year":"2012","journal-title":"Immunol Cell Biol"},{"issue":"3","key":"ref21","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.immuni.2012.01.014","article-title":"Stimulation of HIV-1-specific cytolytic T lymphocytes facilitates elimination of latent viral reservoir after virus reactivation","volume":"36","author":"L Shan","year":"2012","journal-title":"Immunity"},{"issue":"7408","key":"ref22","doi-asserted-by":"crossref","first-page":"482","DOI":"10.1038\/nature11286","article-title":"Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy","volume":"487","author":"NM Archin","year":"2012","journal-title":"Nature"},{"issue":"5","key":"ref23","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1093\/infdis\/jiu155","article-title":"HIV-1 expression within resting CD4+ T cells after multiple doses of vorinostat","volume":"210","author":"NM Archin","year":"2014","journal-title":"J Infect Dis"},{"issue":"10","key":"ref24","doi-asserted-by":"crossref","first-page":"e1004473","DOI":"10.1371\/journal.ppat.1004473","article-title":"Activation of HIV Transcription with Short-Course Vorinostat in HIV-Infected Patients on Suppressive Antiretroviral Therapy","volume":"10","author":"JH Elliott","year":"2014","journal-title":"PLoS Pathog"},{"issue":"1","key":"ref25","doi-asserted-by":"crossref","first-page":"e13","DOI":"10.1016\/S2352-3018(14)70014-1","article-title":"Panobinostat, a histone deacetylase inhibitor, for latent-virus reactivation in HIV-infected patients on suppressive antiretroviral therapy: a phase 1\/2, single group, clinical trial","volume":"1","author":"TA Rasmussen","year":"2014","journal-title":"The Lancet HIV"},{"issue":"4","key":"ref26","doi-asserted-by":"crossref","first-page":"e1004071","DOI":"10.1371\/journal.ppat.1004071","article-title":"Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosing","volume":"10","author":"DG Wei","year":"2014","journal-title":"PLoS Pathog"},{"issue":"8","key":"ref27","doi-asserted-by":"crossref","first-page":"e1004287","DOI":"10.1371\/journal.ppat.1004287","article-title":"Histone deacetylase inhibitors impair the elimination of HIV-infected cells by cytotoxic T-lymphocytes","volume":"10","author":"RB Jones","year":"2014","journal-title":"PLoS Pathog"},{"issue":"4","key":"ref28","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S1473-3099(13)70343-8","article-title":"Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4x: a phase 2 randomised, double-blind, placebo-controlled trial","volume":"14","author":"RB Pollard","year":"2014","journal-title":"Lancet Infect Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/1742-4690-10-41","article-title":"Cell-associated HIV RNA: a dynamic biomarker of viral persistence","volume":"10","author":"AO Pasternak","year":"2013","journal-title":"Retrovirology"},{"issue":"10 Pt 2","key":"ref30","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1111\/trf.12178","article-title":"Comparative analysis of triplex nucleic acid test assays in United States blood donors","volume":"53","author":"SL Stramer","year":"2013","journal-title":"Transfusion"},{"issue":"10","key":"ref31","doi-asserted-by":"crossref","first-page":"4531","DOI":"10.1128\/JCM.41.10.4531-4536.2003","article-title":"New real-time reverse transcriptase-initiated PCR assay with single-copy sensitivity for human immunodeficiency virus type 1 RNA in plasma","volume":"41","author":"S Palmer","year":"2003","journal-title":"Journal of clinical microbiology"},{"issue":"5","key":"ref32","doi-asserted-by":"crossref","first-page":"e1004156","DOI":"10.1371\/journal.ppat.1004156","article-title":"Dynamics of HIV latency and reactivation in a primary CD4+ T cell model","volume":"10","author":"P Mohammadi","year":"2014","journal-title":"PLoS Pathog"},{"issue":"9","key":"ref33","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1038\/nrd4360","article-title":"Histone deacetylases and their inhibitors in cancer, neurological diseases and immune disorders","volume":"13","author":"KJ Falkenberg","year":"2014","journal-title":"Nature reviews Drug discovery"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.canlet.2010.02.024","article-title":"Histone deacetylase inhibitors prevent activation of tumour-reactive NK cells and T cells but do not interfere with their cytolytic effector functions","volume":"295","author":"M Schmudde","year":"2010","journal-title":"Cancer Lett"},{"issue":"7534","key":"ref35","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1038\/nature14053","article-title":"Broad CTL response is required to clear latent HIV-1 due to dominance of escape mutations","volume":"517","author":"K Deng","year":"2015","journal-title":"Nature"},{"issue":"13","key":"ref36","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.2146\/ajhp120163","article-title":"Romidepsin for the treatment of T-cell lymphomas","volume":"70","author":"AL McGraw","year":"2013","journal-title":"American journal of health-system pharmacy: AJHP: official journal of the American Society of Health-System Pharmacists"},{"issue":"7","key":"ref37","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.it.2011.04.001","article-title":"Histone deacetylases as regulators of inflammation and immunity","volume":"32","author":"MR Shakespear","year":"2011","journal-title":"Trends Immunol"},{"issue":"2 Suppl 5","key":"ref38","first-page":"8","article-title":"Analysis of peripheral T-cell lymphoma (PTCL) subtype by race and geography using the Comprehensive Oncology Measures for Peripheral T-Cell Lymphoma Treatment (COMPLETE) dataset","volume":"12","year":"2014","journal-title":"Clinical advances in hematology & oncology: H&O"},{"key":"ref39","article-title":"Evaluation of CYP3A-mediated drug-drug Interactions with romidepsin in patients with advanced cancer","author":"E Laille","year":"2015","journal-title":"J Clin Pharmacol"},{"key":"ref40","unstructured":"Administration USFaD. Drug Development and Drug Interactions 2015 [cited 2015 21 July]. Available from: <ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.fda.gov\/Drugs\/DevelopmentApprovalProcess\/DevelopmentResources\" xlink:type=\"simple\">http:\/\/www.fda.gov\/Drugs\/DevelopmentApprovalProcess\/DevelopmentResources<\/ext-link>."},{"issue":"8","key":"ref41","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1038\/nm.1972","article-title":"HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation","volume":"15","author":"N Chomont","year":"2009","journal-title":"Nat Med"},{"issue":"17","key":"ref42","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1097\/QAD.0b013e32834b9658","article-title":"Impact of treatment with raltegravir during primary or chronic HIV infection on RNA decay characteristics and the HIV viral reservoir","volume":"25","author":"KK Koelsch","year":"2011","journal-title":"Aids"},{"issue":"7","key":"ref43","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.1128\/JCM.00055-08","article-title":"Highly sensitive methods based on seminested real-time reverse transcription-PCR for quantitation of human immunodeficiency virus type 1 unspliced and multiply spliced RNA and proviral DNA","volume":"46","author":"AO Pasternak","year":"2008","journal-title":"J Clin Microbiol"},{"issue":"1\u20132","key":"ref44","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jim.2009.06.008","article-title":"ELDA: extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays","volume":"347","author":"Y Hu","year":"2009","journal-title":"Journal of immunological methods"},{"issue":"5","key":"ref45","doi-asserted-by":"crossref","first-page":"e1003398","DOI":"10.1371\/journal.ppat.1003398","article-title":"Rapid Quantification of the Latent Reservoir for HIV-1 Using a Viral Outgrowth Assay","volume":"9","author":"GM Laird","year":"2013","journal-title":"PLoS Pathog"},{"key":"ref46","first-page":"3","article-title":"Enhanced culture assay for detection and quantitation of latently infected, resting CD4+ T-cells carrying replication-competent virus in HIV-1-infected individuals","volume":"304","author":"JD Siliciano","year":"2005","journal-title":"Methods Mol Biol"},{"issue":"2","key":"ref47","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/nm.3445","article-title":"HIV-1 persistence in CD4+ T cells with stem cell-like properties","volume":"20","author":"MJ Buzon","year":"2014","journal-title":"Nat Med"},{"issue":"6","key":"ref48","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1128\/AAC.46.6.1896-1905.2002","article-title":"Emergence of resistant human immunodeficiency virus type 1 in patients receiving fusion inhibitor (T-20) monotherapy","volume":"46","author":"X Wei","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"issue":"8","key":"ref49","doi-asserted-by":"crossref","first-page":"875","DOI":"10.4161\/epi.20983","article-title":"Methods for the analysis of histone H3 and H4 acetylation in blood","volume":"7","author":"L Rigby","year":"2012","journal-title":"Epigenetics"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1005142","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T13:55:10Z","timestamp":1567173310000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1005142"}},"subtitle":[],"editor":[{"given":"Robert F.","family":"Siliciano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,9,17]]},"references-count":49,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2015,9,17]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1005142","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725803090.793541817","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,17]]}}}