{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T11:09:49Z","timestamp":1787656189613,"version":"build-2736575974"},"reference-count":76,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T00:00:00Z","timestamp":1472515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft (DE)","award":["Br1744\/11-2"],"award-info":[{"award-number":["Br1744\/11-2"]}]},{"DOI":"10.13039\/100010127","name":"J\u00fcrgen Manchot Stiftung","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010127","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1005853","type":"journal-article","created":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T17:28:51Z","timestamp":1472578131000},"page":"e1005853","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":43,"title":["Overexpression of Differentially Expressed Genes Identified in Non-pathogenic and Pathogenic Entamoeba histolytica Clones Allow Identification of New Pathogenicity Factors Involved in Amoebic Liver Abscess Formation"],"prefix":"10.1371","volume":"12","author":[{"given":"Martin","family":"Meyer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Fehling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Matthiesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Lorenzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Schuldt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Bernin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareen","family":"Zaruba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinna","family":"Lender","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-9241","authenticated-orcid":true,"given":"Thomas","family":"Ernst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald","family":"Ittrich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Roeder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egbert","family":"Tannich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannelore","family":"Lotter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iris","family":"Bruchhaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,8,30]]},"reference":[{"key":"ref1","first-page":"126","article-title":"Atlas of human infectios diseases","author":"HFL Wertheim","year":"2012"},{"issue":"10","key":"ref2","doi-asserted-by":"crossref","first-page":"4745","DOI":"10.1128\/JCM.41.10.4745-4750.2003","article-title":"Longitudinal study of intestinal Entamoeba histolytica infections in asymptomatic adult carriers","volume":"41","author":"J Blessmann","year":"2003","journal-title":"J Clin Microbiol"},{"issue":"2","key":"ref3","doi-asserted-by":"crossref","first-page":"e00072","DOI":"10.1128\/mBio.00072-13","article-title":"Overexpression of specific cysteine peptidases confers pathogenicity to a nonpathogenic Entamoeba histolytica clone","volume":"4","author":"J Matthiesen","year":"2013","journal-title":"MBio"},{"issue":"3","key":"ref4","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1046\/j.1365-2958.1999.01607.x","article-title":"Antisense inhibition of amoebapore expression in Entamoeba histolytica causes a decrease in amoebic virulence","volume":"34","author":"R Bracha","year":"1999","journal-title":"Mol Microbiol"},{"issue":"2","key":"ref5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1128\/EC.2.2.295-305.2003","article-title":"Transcriptional silencing of an amoebapore gene in Entamoeba histolytica: molecular analysis and effect on pathogenicity","volume":"2","author":"R Bracha","year":"2003","journal-title":"Eukaryot Cell"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1128\/IAI.67.1.421-422.1999","article-title":"Antisense inhibition of expression of cysteine proteinases affects <italic>Entamoeba histolytica<\/italic>-induced formation of liver abscess in hamsters","volume":"67","author":"S Ankri","year":"1999","journal-title":"Infect Immun"},{"issue":"1","key":"ref7","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1128\/IAI.74.1.340-351.2006","article-title":"Identification of differentially expressed genes in virulent and nonvirulent Entamoeba species: potential implications for amebic pathogenesis","volume":"74","author":"RC MacFarlane","year":"2006","journal-title":"Infect Immun"},{"issue":"1","key":"ref8","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.molbiopara.2006.10.014","article-title":"Transcriptomic comparison of two Entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin","volume":"151","author":"PH Davis","year":"2007","journal-title":"Mol Biochem Parasitol"},{"issue":"6","key":"ref9","doi-asserted-by":"crossref","first-page":"1426","DOI":"10.1111\/j.1462-5822.2006.00882.x","article-title":"Identification of developmentally regulated genes in Entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite","volume":"9","author":"GM Ehrenkaufer","year":"2007","journal-title":"Cell Microbiol"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1111\/j.1365-2958.2006.05344.x","article-title":"Comparative proteomic analysis of two Entamoeba histolytica strains with different virulence phenotypes identifies peroxiredoxin as an important component of amoebic virulence","volume":"61","author":"PH Davis","year":"2006","journal-title":"Mol Microbiol"},{"issue":"17","key":"ref11","doi-asserted-by":"crossref","first-page":"4107","DOI":"10.1002\/pmic.200900022","article-title":"Comparison of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties","volume":"9","author":"L Biller","year":"2009","journal-title":"Proteomics"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1471-2164-11-63","article-title":"Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties","volume":"11","author":"L Biller","year":"2010","journal-title":"BMC Genomics"},{"issue":"1","key":"ref13","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1074\/mcp.M113.031393","article-title":"The Cell Surface Proteome of Entamoeba histolytica","volume":"13","author":"L Biller","year":"2014","journal-title":"Mol Cell Proteomics"},{"issue":"9","key":"ref14","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1093\/jac\/dkr238","article-title":"Mechanism of trifluoromethionine resistance in Entamoeba histolytica","volume":"66","author":"GM Penuliar","year":"2011","journal-title":"J Antimicrob Chemother"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1093\/jac\/dkr484","article-title":"Transcriptional and functional analysis of trifluoromethionine resistance in Entamoeba histolytica","volume":"67","author":"GM Penuliar","year":"2012","journal-title":"J Antimicrob Chemother"},{"issue":"32","key":"ref16","doi-asserted-by":"crossref","first-page":"24223","DOI":"10.1016\/S0021-9258(20)80514-6","article-title":"Structural analysis of the light subunit of the Entamoeba histolytica galactose-specific adherence lectin","volume":"268","author":"JJ McCoy","year":"1993","journal-title":"J Biol Chem"},{"issue":"5","key":"ref17","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1007\/BF00731279","article-title":"Use of monoclonal anti-light subunit antibodies to study the structure and function of the Entamoeba histolytica Gal\/GalNAc adherence lectin","volume":"11","author":"JJ McCoy","year":"1994","journal-title":"Glycoconj J"},{"issue":"12","key":"ref18","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1139\/W10-088","article-title":"Effect of phosphatidylcholine-cholesterol liposomes on Entamoeba histolytica virulence","volume":"56","author":"J Serrano-Luna","year":"2010","journal-title":"Can J Microbiol"},{"key":"ref19","first-page":"173","article-title":"Proteinase activity &amp; virulence of Entamoeba histolytica on passage through hamster liver","volume":"107","author":"S Gupta","year":"1998","journal-title":"Indian J Med Res"},{"issue":"5592","key":"ref20","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1038\/265341a0","article-title":"Virulence and toxicity of axenic Entamoeba histolytica","volume":"265","author":"HJ Bos","year":"1977","journal-title":"Nature"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/BF00932263","article-title":"Phagocytosis and proteinase activity are not related to pathogenicity of E. histolytica","volume":"79","author":"SL Monfort","year":"1993","journal-title":"Parasitol Res"},{"issue":"2","key":"ref22","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1006\/expr.2000.4554","article-title":"Entamoeba histolytica: diminution of erythrophagocytosis, phospholipase A(2), and hemolytic activities is related to virulence impairment in long-term axenic cultures","volume":"96","author":"MT Gonzalez-Garza","year":"2000","journal-title":"Exp Parasitol"},{"issue":"1","key":"ref23","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1128\/IAI.74.1.118-124.2006","article-title":"Sexual dimorphism in the control of amebic liver abscess in a mouse model of disease","volume":"74","author":"H Lotter","year":"2006","journal-title":"Infect Immun"},{"issue":"1","key":"ref24","doi-asserted-by":"crossref","first-page":"e1003096","DOI":"10.1371\/journal.ppat.1003096","article-title":"TNFalpha-mediated liver destruction by Kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection","volume":"9","author":"E Helk","year":"2013","journal-title":"PLoS pathog"},{"issue":"5","key":"ref25","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.jhep.2016.01.013","article-title":"IL-23 prevents IL-13-dependent tissue repair associated with Ly6C(lo) monocytes in Entamoeba histolytica-induced liver damage","volume":"64","author":"J Noll","year":"2016","journal-title":"J Hepatol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1146\/annurev-micro-091313-103550","article-title":"Regulation of virulence of Entamoeba histolytica","volume":"68","author":"C Marie","year":"2014","journal-title":"Annu Rev Microbiol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.3389\/fimmu.2016.00175","article-title":"Immune Response of Amebiasis and Immune Evasion by Entamoeba histolytica","volume":"7","author":"K Nakada-Tsukui","year":"2016","journal-title":"Front Immunol"},{"issue":"2","key":"ref28","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1128\/IAI.39.2.528-531.1983","article-title":"Correlation of virulence and collagenolytic activity in Entamoeba histolytica","volume":"39","author":"H Gadasi","year":"1983","journal-title":"Infect Immun"},{"issue":"2","key":"ref29","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/0014-4894(90)90022-5","article-title":"Entamoeba histolytica: correlation of the cytopathic effect of virulent trophozoites with secretion of a cysteine proteinase","volume":"71","author":"WE Keene","year":"1990","journal-title":"Exp Parasitol"},{"issue":"6","key":"ref30","doi-asserted-by":"crossref","first-page":"1488","DOI":"10.1016\/S0016-5085(84)80163-8","article-title":"Relationship of cytotoxins of axenically cultivated Entamoeba histolytica to virulence","volume":"86","author":"WB Lushbaugh","year":"1984","journal-title":"Gastroenterology"},{"issue":"3","key":"ref31","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/0014-4894(85)90088-8","article-title":"Entamoeba histolytica: purification of cathepsin B","volume":"59","author":"WB Lushbaugh","year":"1985","journal-title":"Exp Parasitol"},{"issue":"10","key":"ref32","doi-asserted-by":"crossref","first-page":"4150","DOI":"10.1128\/IAI.63.10.4150-4153.1995","article-title":"Interaction of laminin with Entamoeba histolytica cysteine proteinases and its effect on amebic pathogenesis","volume":"63","author":"E Li","year":"1995","journal-title":"Infect Immun"},{"issue":"4","key":"ref33","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1128\/IAI.63.4.1587-1590.1995","article-title":"Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice","volume":"63","author":"SL Stanley Jr.","year":"1995","journal-title":"Infect Immun"},{"issue":"3","key":"ref34","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1046\/j.1365-2958.2000.02037.x","article-title":"Entamoeba histolytica cysteine proteinases with interleukin-1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis","volume":"37","author":"Z Zhang","year":"2000","journal-title":"Mol Microbiol"},{"issue":"1","key":"ref35","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.molbiopara.2006.04.009","article-title":"Increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of EhCP5 for the pathogenicity of Entamoeba histolytica","volume":"149","author":"M Tillack","year":"2006","journal-title":"Mol Biochem Parasitol"},{"issue":"11","key":"ref36","doi-asserted-by":"crossref","first-page":"e551","DOI":"10.1371\/journal.pntd.0000551","article-title":"An ex-vivo human intestinal model to study Entamoeba histolytica pathogenesis","volume":"3","author":"D Bansal","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"issue":"5","key":"ref37","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1462-5822.2012.01752.x","article-title":"Newly visualized fibrillar collagen scaffolds dictate Entamoeba histolytica invasion route in the human colon","volume":"14","author":"R Thibeaux","year":"2012","journal-title":"Cell Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"5142","DOI":"10.1038\/ncomms6142","article-title":"The parasite Entamoeba histolytica exploits the activities of human matrix metalloproteinases to invade colonic tissue","volume":"5","author":"R Thibeaux","year":"2014","journal-title":"Nat Commun"},{"issue":"4","key":"ref39","doi-asserted-by":"crossref","first-page":"e1005579","DOI":"10.1371\/journal.ppat.1005579","article-title":"Entamoeba histolytica Cysteine Proteinase 5 Evokes mucin exocytosis from colonic goblet cells via alphavbeta3 integrin","volume":"12","author":"S Cornick","year":"2016","journal-title":"PLoS Pathog"},{"issue":"5","key":"ref40","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1084\/jem.148.5.1137","article-title":"Surface properties of Entamoeba: increased rates of human erythrocyte phagocytosis in pathogenic strains","volume":"148","author":"D Trissl","year":"1978","journal-title":"J Exp Med"},{"issue":"1","key":"ref41","first-page":"11","article-title":"Study of Entamoeba histolytica isolates from Calcutta with special reference to their zymodeme, animal pathogenicity and erythrophagocytosis","volume":"9","author":"RM Mukherjee","year":"1991","journal-title":"J Diarrhoeal Dis Res"},{"issue":"5","key":"ref42","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1084\/jem.158.5.1511","article-title":"Entamoeba histolytica. Phagocytosis as a virulence factor","volume":"158","author":"E Orozco","year":"1983","journal-title":"J Exp Med"},{"issue":"6","key":"ref43","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/0020-7519(85)90012-8","article-title":"Differences in adhesion, phagocytosis and virulence of clones from Entamoeba histolytica, strain HM1:IMSS","volume":"15","author":"E Orozco","year":"1985","journal-title":"Int J Parasitol"},{"issue":"2","key":"ref44","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/0035-9203(92)90555-Q","article-title":"Entamoeba histolytica: erythrophagocytosis, collagenolysis, and liver abscess production as virulence markers","volume":"86","author":"V Tsutsumi","year":"1992","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.mib.2015.07.009","article-title":"Taking a bite: Amoebic trogocytosis in Entamoeba histolytica and beyond","volume":"28","author":"KS Ralston","year":"2015","journal-title":"Curr Opin Microbiol"},{"issue":"7497","key":"ref46","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1038\/nature13242","article-title":"Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion","volume":"508","author":"KS Ralston","year":"2014","journal-title":"Nature"},{"issue":"4","key":"ref47","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.arcmed.2007.01.003","article-title":"Transcriptional upregulation of genes related to virulence activation in Entamoeba histolytica","volume":"38","author":"I Balderas-Renteria","year":"2007","journal-title":"Archives of medical research"},{"issue":"3","key":"ref48","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/j.1550-7408.1988.tb04107.x","article-title":"Pathogenic and non-pathogenic Entamoeba: pore formation and hemolytic activity","volume":"35","author":"F Keller","year":"1988","journal-title":"J Protozool"},{"issue":"2","key":"ref49","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/CMR.13.2.196-206.2000","article-title":"Cysteine proteinases and the pathogenesis of amebiasis","volume":"13","author":"X Que","year":"2000","journal-title":"Clin Microbiol Rev"},{"issue":"2","key":"ref50","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00239748","article-title":"Cytopathogenicity of Entamoeba histolytica: the role of amebic adherence and contact-dependent cytolysis in pathogenesis","volume":"3","author":"WA Petri Jr.","year":"1987","journal-title":"Eur J Epidemiol"},{"issue":"5","key":"ref51","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/EC.00346-09","article-title":"Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica","volume":"9","author":"H Moreno","year":"2010","journal-title":"Eukaryotic Cell"},{"issue":"15","key":"ref52","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1016\/j.micinf.2012.05.014","article-title":"Leishmania virulence factors: focus on the metalloprotease GP63","volume":"14","author":"M Olivier","year":"2012","journal-title":"Microbes Infect"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"72","DOI":"10.3389\/fcimb.2012.00072","article-title":"Impact of Leishmania metalloprotease GP63 on macrophage signaling","volume":"2","author":"A Isnard","year":"2012","journal-title":"Front Cell Infect Microbiol"},{"issue":"6","key":"ref54","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.1128\/IAI.06389-11","article-title":"Control of Entamoeba histolytica adherence involves metallosurface protease 1, an M8 family surface metalloprotease with homology to leishmanolysin","volume":"80","author":"JE Teixeira","year":"2012","journal-title":"Infect Immun"},{"issue":"6","key":"ref55","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1016\/j.ygeno.2005.08.004","article-title":"The phylogenetic analysis of tetraspanins projects the evolution of cell-cell interactions from unicellular to multicellular organisms","volume":"86","author":"S Huang","year":"2005","journal-title":"Genomics"},{"issue":"10","key":"ref56","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1038\/nrm1736","article-title":"Tetraspanin functions and associated microdomains","volume":"6","author":"ME Hemler","year":"2005","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"8","key":"ref57","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1111\/cmi.12431","article-title":"The C-terminal tail of tetraspanin proteins regulates their intracellular distribution in the parasite Trichomonas vaginalis","volume":"17","author":"VM Coceres","year":"2015","journal-title":"Cell Microbiol"},{"issue":"4","key":"ref58","doi-asserted-by":"crossref","first-page":"e415","DOI":"10.1371\/journal.pntd.0000415","article-title":"Proteomic comparison of Entamoeba histolytica and Entamoeba dispar and the role of E. histolytica alcohol dehydrogenase 3 in virulence","volume":"3","author":"PH Davis","year":"2009","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"ref59","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1038\/35052055","article-title":"Rab proteins as membrane organizers","volume":"2","author":"M Zerial","year":"2001","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"3","key":"ref60","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.exppara.2005.02.021","article-title":"The diversity of Rab GTPases in Entamoeba histolytica","volume":"110","author":"Y Saito-Nakano","year":"2005","journal-title":"Exp Parasitol"},{"issue":"7","key":"ref61","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1111\/j.1462-5822.2007.00915.x","article-title":"Two Rab7 isotypes, EhRab7A and EhRab7B, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite Entamoeba histolytica","volume":"9","author":"Y Saito-Nakano","year":"2007","journal-title":"Cell Microbiol"},{"issue":"47","key":"ref62","doi-asserted-by":"crossref","first-page":"49497","DOI":"10.1074\/jbc.M403987200","article-title":"Rab5-associated vacuoles play a unique role in phagocytosis of the enteric protozoan parasite Entamoeba histolytica","volume":"279","author":"Y Saito-Nakano","year":"2004","journal-title":"J Biol Chem"},{"issue":"11","key":"ref63","doi-asserted-by":"crossref","first-page":"5294","DOI":"10.1091\/mbc.E05-04-0283","article-title":"A retromerlike complex is a novel Rab7 effector that is involved in the transport of the virulence factor cysteine protease in the enteric protozoan parasite Entamoeba histolytica","volume":"16","author":"K Nakada-Tsukui","year":"2005","journal-title":"Mol Biol Cell"},{"issue":"3","key":"ref64","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00436-005-0079-6","article-title":"Membrane trafficking as a virulence mechanism of the enteric protozoan parasite Entamoeba histolytica","volume":"98","author":"T Nozaki","year":"2006","journal-title":"Parasitol Res"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/0035-9203(78)90144-X","article-title":"A new medium for axenic cultivation of <italic>Entamoeba histolytica<\/italic> and other <italic>Entamoeba<\/italic>","volume":"72","author":"LS Diamond","year":"1978","journal-title":"Trans R Soc Trop Med Hyg"},{"issue":"7028","key":"ref66","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/nature03291","article-title":"The genome of the protist parasite Entamoeba histolytica","volume":"433","author":"B Loftus","year":"2005","journal-title":"Nature"},{"issue":"10","key":"ref67","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1084\/jem.185.10.1793","article-title":"Identification of an epitope on the Entamoeba histolytica 170-kD lectin conferring antibody-mediated protection against invasive amebiasis","volume":"185","author":"H Lotter","year":"1997","journal-title":"J Exp Med"},{"issue":"10","key":"ref68","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1097\/RLI.0000000000000175","article-title":"Magnetic Resonance Imaging of Pathogenic Protozoan Parasite Entamoeba histolytica Labeled With Superparamagnetic Iron Oxide Nanoparticles","volume":"50","author":"TM Ernst","year":"2015","journal-title":"Invest Radiol"},{"issue":"4","key":"ref69","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"KJ Livak","year":"2001","journal-title":"Methods"},{"issue":"4","key":"ref70","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"B Langmead","year":"2012","journal-title":"Nat Methods"},{"issue":"1","key":"ref71","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: tool for the unification of biology. The Gene Ontology Consortium","volume":"25","author":"M Ashburner","year":"2000","journal-title":"Nat Genet"},{"issue":"Pt 5","key":"ref72","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1017\/S0031182000077040","article-title":"Spontaneous release of cysteine proteinases but not of pore-forming peptides by viable Entamoeba histolytica","volume":"111","author":"M Leippe","year":"1995","journal-title":"Parasitology"},{"issue":"2","key":"ref73","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0166-6851(99)00194-2","article-title":"Two major &apos;higher molecular mass proteinases&apos; of Entamoeba histolytica are identified as cysteine proteinases 1 and 2","volume":"105","author":"A Hellberg","year":"2000","journal-title":"Mol Biochem Parasitol"},{"issue":"2","key":"ref74","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1084\/jem.160.2.353","article-title":"Virulence of Entamoeba histolytica trophozoites. Effects of bacteria, microaerobic conditions, and metronidazole","volume":"160","author":"R Bracha","year":"1984","journal-title":"J Exp Med"},{"issue":"15","key":"ref75","doi-asserted-by":"crossref","first-page":"7095","DOI":"10.1073\/pnas.91.15.7095","article-title":"Transfection and transient expression of chloramphenicol acetyltransferase gene in the protozoan parasite Entamoeba histolytica","volume":"91","author":"R Nickel","year":"1994","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"19","key":"ref76","doi-asserted-by":"crossref","first-page":"8975","DOI":"10.1073\/pnas.92.19.8975","article-title":"Transfection and continuous expression of heterologous genes in the protozoan parasite Entamoeba histolytica","volume":"92","author":"L Hamann","year":"1995","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1005853","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T11:44:15Z","timestamp":1657194255000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1005853"}},"subtitle":[],"editor":[{"given":"Patricia J.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,8,30]]},"references-count":76,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2016,8,30]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1005853","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,30]]}}}