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Desire","year":"2001","journal-title":"Journal of clinical microbiology"},{"issue":"13","key":"ref76","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1096\/fj.07-8726com","article-title":"piggyBac transposon mediated transgenesis of the human blood fluke, Schistosoma mansoni","volume":"21","author":"ME Morales","year":"2007","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"issue":"2","key":"ref77","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S1471-4922(02)00061-2","article-title":"Mobile genetic elements colonizing the genomes of metazoan parasites","volume":"19","author":"PJ Brindley","year":"2003","journal-title":"Trends in parasitology"},{"issue":"6","key":"ref78","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0301-472X(02)00806-8","article-title":"Gene quantification using real-time quantitative PCR: an emerging technology hits the 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