{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:14:59Z","timestamp":1791299699846,"version":"4.1.0"},"reference-count":66,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2016,10,14]],"date-time":"2016-10-14T00:00:00Z","timestamp":1476403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","award":["1I01BX001183-01"],"award-info":[{"award-number":["1I01BX001183-01"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["ARZT-570410-A-02-139"],"award-info":[{"award-number":["ARZT-570410-A-02-139"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1005946","type":"journal-article","created":{"date-parts":[[2016,10,14]],"date-time":"2016-10-14T17:42:17Z","timestamp":1476466937000},"page":"e1005946","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":56,"title":["A Clostridium difficile Cell Wall Glycopolymer Locus Influences Bacterial Shape, Polysaccharide Production and Virulence"],"prefix":"10.1371","volume":"12","author":[{"given":"Michele","family":"Chu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J. G.","family":"Mallozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0139-2232","authenticated-orcid":true,"given":"Bryan P.","family":"Roxas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Bertolo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario A.","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al","family":"Agellon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. K.","family":"Viswanathan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayatri","family":"Vedantam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,10,14]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.4161\/gmic.1.4.12706","article-title":"<italic>Clostridium difficile<\/italic> infection: An overview of the disease and its pathogenesis, epidemiology and interventions","volume":"1","author":"VK Viswanathan","year":"2010","journal-title":"Gut Microbes"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1097\/MCC.0b013e328324e6ad","article-title":"<italic>Clostridium difficile<\/italic> infection: current perspectives","volume":"15","author":"J Janka","year":"2009","journal-title":"Curr Opin Crit Care"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/j.1863-2378.2010.01352.x","article-title":"The Ecology and Pathobiology of <italic>Clostridium difficile<\/italic> Infections: An Interdisciplinary Challenge","volume":"58","author":"ER Dubberke","year":"2011","journal-title":"Zoonoses and Public Health"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"234","DOI":"10.4161\/gmic.1.4.12706","article-title":"<italic>Clostridium difficile<\/italic> infection: An overview of the disease and its pathogenesis, epidemiology and interventions","volume":"1","author":"VK Viswanathan","year":"2010","journal-title":"Gut Microbes"},{"key":"ref5","unstructured":"(2013) Antibiotic Resistance Threats in the United States. Centers for Disease Control and Prevention."},{"issue":"Suppl 2","key":"ref6","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1093\/cid\/cis335","article-title":"Burden of <italic>Clostridium difficile<\/italic> on the healthcare system","volume":"55","author":"ER Dubberke","year":"2012","journal-title":"Clin Infect Dis"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1038\/nature07822","article-title":"Toxin B is essential for virulence of <italic>Clostridium difficile<\/italic>","volume":"458","author":"D Lyras","year":"2009","journal-title":"Nature"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1038\/nature09397","article-title":"The role of toxin A and toxin B in <italic>Clostridium difficile<\/italic> infection","volume":"467","author":"SA Kuehne","year":"2010","journal-title":"Nature"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anaerobe.2013.05.005","article-title":"A clinical and epidemiological review of non-toxigenic <italic>Clostridium difficile<\/italic>","volume":"22","author":"M Natarajan","year":"2013","journal-title":"Anaerobe"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"121","DOI":"10.4161\/gmic.19399","article-title":"<italic>Clostridium difficile<\/italic> infection: Toxins and non-toxin virulence factors, and their contributions to disease establishment and host response","volume":"3","author":"G Vedantam","year":"2012","journal-title":"Gut Microbes"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1146\/annurev-micro-092412-155620","article-title":"Wall teichoic acids of Gram-positive bacteria","volume":"67","author":"S Brown","year":"2013","journal-title":"Annu Rev Microbiol"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1146\/annurev.micro.50.1.285","article-title":"The biochemistry and genetics of capsular polysaccharide production in bacteria","volume":"50","author":"IS Roberts","year":"1996","journal-title":"Annu Rev Microbiol"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0882-4010(90)90088-8","article-title":"Detection of capsule in strains of <italic>Clostridium difficile<\/italic> of varying virulence and toxigenicity","volume":"9","author":"HA Davies","year":"1990","journal-title":"Microb Pathog"},{"key":"ref14","first-page":"295","article-title":"Capsule-like structures in <italic>Clostridium difficile<\/italic> strains","volume":"14","author":"L Baldassarri","year":"1991","journal-title":"Microbiologica"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.carres.2008.01.002","article-title":"<italic>Clostridium difficile<\/italic> cell-surface polysaccharides composed of pentaglycosyl and hexaglycosyl phosphate repeating units","volume":"343","author":"J Ganeshapillai","year":"2008","journal-title":"Carbohydr Res"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.carres.2012.02.002","article-title":"Structural characterization of surface glycans from <italic>Clostridium difficile<\/italic>","volume":"354","author":"CW Reid","year":"2012","journal-title":"Carbohydr Res"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"7159","DOI":"10.1039\/c3cc43545h","article-title":"Glycan arrays containing synthetic <italic>Clostridium difficile<\/italic> lipoteichoic acid oligomers as tools toward a carbohydrate vaccine","volume":"49","author":"CE Martin","year":"2013","journal-title":"Chem Commun (Camb)"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.carres.2012.03.032","article-title":"<italic>Clostridium difficile<\/italic> carbohydrates: glucan in spores, PSII common antigen in cells, immunogenicity of PSII in swine and synthesis of a dual <italic>C<\/italic>. <italic>difficile<\/italic>-ETEC conjugate vaccine","volume":"354","author":"L Bertolo","year":"2012","journal-title":"Carbohydr Res"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1021\/cb300221f","article-title":"Phosphorylation of the synthetic hexasaccharide repeating unit is essential for the induction of antibodies to <italic>Clostridium difficile<\/italic> PSII cell wall polysaccharide","volume":"7","author":"R Adamo","year":"2012","journal-title":"ACS Chem Biol"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1007\/s10719-013-9489-3","article-title":"Investigating the candidacy of a lipoteichoic acid-based glycoconjugate as a vaccine to combat <italic>Clostridium difficile<\/italic> infection","volume":"30","author":"AD Cox","year":"2013","journal-title":"Glycoconj J"},{"key":"ref21","article-title":"<italic>Clostridium difficile<\/italic> surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII","author":"SE Willing","year":"2015","journal-title":"Mol Microbiol"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"7856","DOI":"10.1128\/JB.00837-07","article-title":"Predicted functions and linkage specificities of the products of the <italic>Streptococcus pneumoniae<\/italic> capsular biosynthetic loci","volume":"189","author":"DM Aanensen","year":"2007","journal-title":"J Bacteriol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1089\/mdr.2012.0026","article-title":"Biosynthesis of teichoic acids in <italic>Streptococcus pneumoniae<\/italic> and closely related species: lessons from genomes","volume":"18","author":"D Denapaite","year":"2012","journal-title":"Microb Drug Resist"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1111\/j.1365-2958.2006.05315.x","article-title":"Construction and analysis of chromosomal <italic>Clostridium difficile<\/italic> mutants","volume":"61","author":"JR O'Connor","year":"2006","journal-title":"Mol Microbiol"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"3024","DOI":"10.1128\/JCM.01013-11","article-title":"Use of modified PCR ribotyping for direct detection of <italic>Clostridium difficile<\/italic> ribotypes in stool samples","volume":"49","author":"S Janezic","year":"2011","journal-title":"J Clin Microbiol"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.mimet.2009.10.018","article-title":"The ClosTron: Mutagenesis in Clostridium refined and streamlined","volume":"80","author":"JT Heap","year":"2010","journal-title":"J Microbiol Methods"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.jmb.2003.12.009","article-title":"Use of computer-designed group II introns to disrupt <italic>Escherichia coli<\/italic> DExH\/D-box protein and DNA helicase genes","volume":"336","author":"J Perutka","year":"2004","journal-title":"J Mol Biol"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.mimet.2009.05.004","article-title":"A modular system for Clostridium shuttle plasmids","volume":"78","author":"JT Heap","year":"2009","journal-title":"J Microbiol Methods"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"27483","DOI":"10.1074\/jbc.M111.263889","article-title":"<italic>Clostridium difficile<\/italic> has two parallel and essential Sec secretion systems","volume":"286","author":"RP Fagan","year":"2011","journal-title":"J Biol Chem"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/978-1-60327-365-7_11","article-title":"ClosTron-targeted mutagenesis","volume":"646","author":"JT Heap","year":"2010","journal-title":"Methods Mol Biol"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1046\/j.1365-2958.2002.03134.x","article-title":"Conjugative transfer of clostridial shuttle vectors from <italic>Escherichia coli<\/italic> to <italic>Clostridium difficile<\/italic> through circumvention of the restriction barrier","volume":"46","author":"D Purdy","year":"2002","journal-title":"Mol Microbiol"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"4653","DOI":"10.1128\/IAI.73.8.4653-4667.2005","article-title":"Illustration of pneumococcal polysaccharide capsule during adherence and invasion of epithelial cells","volume":"73","author":"S Hammerschmidt","year":"2005","journal-title":"Infect Immun"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/1471-2164-9-75","article-title":"The RAST Server: rapid annotations using subsystems technology","volume":"9","author":"RK Aziz","year":"2008","journal-title":"BMC Genomics"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"D36","DOI":"10.1093\/nar\/gks1195","article-title":"GenBank","volume":"41","author":"DA Benson","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1099\/jmm.0.030452-0","article-title":"Reannotation of the genome sequence of <italic>Clostridium difficile<\/italic> strain 630","volume":"60","author":"M Monot","year":"2011","journal-title":"J Med Microbiol"},{"key":"ref36","first-page":"164","article-title":"PHYLIP\u2014Phylogeny Inference Package (Version 3.2)","volume":"5","author":"J Felsenstein","year":"1989","journal-title":"Cladistics"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"6988","DOI":"10.1128\/JB.00466-09","article-title":"The <italic>rkp<\/italic>-1 cluster is required for secretion of Kdo homopolymeric capsular polysaccharide in <italic>Sinorhizobium meliloti<\/italic> strain Rm1021","volume":"191","author":"MG Muller","year":"2009","journal-title":"J Bacteriol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/978-1-60327-365-7_8","article-title":"Dissecting the cell surface","volume":"646","author":"R Fagan","year":"2010","journal-title":"Methods Mol Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1128\/JCM.16.6.1123-1126.1982","article-title":"Demonstration of age-dependent capsular material on <italic>Pasteurella haemolytica<\/italic> serotype 1","volume":"16","author":"RE Corstvet","year":"1982","journal-title":"J Clin Microbiol"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.anaerobe.2012.09.004","article-title":"<italic>Clostridium difficile<\/italic> clinical isolates exhibit variable susceptibility and proteome alterations upon exposure to mammalian cationic antimicrobial peptides","volume":"18","author":"R McQuade","year":"2012","journal-title":"Anaerobe"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"e0124971","DOI":"10.1371\/journal.pone.0124971","article-title":"The <italic>Clostridium difficile<\/italic> Protease Cwp84 Modulates both Biofilm Formation and Cell-Surface Properties","volume":"10","author":"V Pantaleon","year":"2015","journal-title":"PLoS One"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"e78404","DOI":"10.1371\/journal.pone.0078404","article-title":"Surface-layer protein A (SlpA) is a major contributor to host-cell adherence of <italic>Clostridium difficile<\/italic>","volume":"8","author":"MM Merrigan","year":"2013","journal-title":"PLoS One"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1093\/bioinformatics\/bti448","article-title":"False discovery rate, sensitivity and sample size for microarray studies","volume":"21","author":"Y Pawitan","year":"2005","journal-title":"Bioinformatics"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/cbic.200900557","article-title":"Wall teichoic acid function, biosynthesis, and inhibition","volume":"11","author":"JG Swoboda","year":"2010","journal-title":"Chembiochem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.1128\/JB.01938-06","article-title":"Mutations blocking side chain assembly, polymerization, or transport of a Wzy-dependent <italic>Streptococcus pneumoniae<\/italic> capsule are lethal in the absence of suppressor mutations and can affect polymer transfer to the cell wall","volume":"189","author":"B Xayarath","year":"2007","journal-title":"J Bacteriol"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"6479","DOI":"10.1128\/JB.01135-12","article-title":"Genetic and biochemical characterizations of enzymes involved in <italic>Streptococcus pneumoniae<\/italic> serotype 2 capsule synthesis demonstrate that Cps2T (WchF) catalyzes the committed step by addition of beta1-4 rhamnose, the second sugar residue in the repeat unit","volume":"194","author":"DB James","year":"2012","journal-title":"J Bacteriol"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"4183","DOI":"10.1128\/JB.00197-06","article-title":"Lesions in teichoic acid biosynthesis in <italic>Staphylococcus aureus<\/italic> lead to a lethal gain of function in the otherwise dispensable pathway","volume":"188","author":"MA D'Elia","year":"2006","journal-title":"J Bacteriol"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1146\/annurev.micro.62.081307.162944","article-title":"Capsules of <italic>Streptococcus pneumoniae<\/italic> and other bacteria: paradigms for polysaccharide biosynthesis and regulation","volume":"65","author":"J Yother","year":"2011","journal-title":"Annu Rev Microbiol"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"4931","DOI":"10.1038\/emboj.2011.358","article-title":"A widespread family of bacterial cell wall assembly proteins","volume":"30","author":"Y Kawai","year":"2011","journal-title":"EMBO J"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"4650","DOI":"10.1128\/JB.00544-13","article-title":"<italic>Staphylococcus aureus<\/italic> mutants lacking the LytR-CpsA-Psr family of enzymes release cell wall teichoic acids into the extracellular medium","volume":"195","author":"YG Chan","year":"2013","journal-title":"J Bacteriol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"15680","DOI":"10.1074\/jbc.M114.567669","article-title":"The capsular polysaccharide of <italic>Staphylococcus aureus<\/italic> is attached to peptidoglycan by the LytR-CpsA-Psr (LCP) family of enzymes","volume":"289","author":"YG Chan","year":"2014","journal-title":"J Biol Chem"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1089\/mdr.2011.0232","article-title":"Attachment of capsular polysaccharide to the cell wall in <italic>Streptococcus pneumoniae<\/italic>","volume":"18","author":"A Eberhardt","year":"2012","journal-title":"Microb Drug Resist"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1111\/mmi.12780","article-title":"Lethality of sortase depletion in <italic>Actinomyces oris<\/italic> caused by excessive membrane accumulation of a surface glycoprotein","volume":"94","author":"C Wu","year":"2014","journal-title":"Mol Microbiol"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1128\/JB.02364-14","article-title":"LytR-CpsA-Psr enzymes as determinants of <italic>Bacillus anthracis<\/italic> secondary cell wall polysaccharide assembly","volume":"197","author":"M Liszewski Zilla","year":"2015","journal-title":"J Bacteriol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"7425","DOI":"10.1128\/JB.187.21.7425-7433.2005","article-title":"CpsE from type 2 <italic>Streptococcus pneumoniae<\/italic> catalyzes the reversible addition of glucose-1-phosphate to a polyprenyl phosphate acceptor, initiating type 2 capsule repeat unit formation","volume":"187","author":"RT Cartee","year":"2005","journal-title":"J Bacteriol"},{"key":"ref56","article-title":"CLSI Document M07-A9","year":"2012"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"15553","DOI":"10.1073\/pnas.0808352105","article-title":"Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in <italic>Escherichia coli<\/italic>","volume":"105","author":"N Ruiz","year":"2008","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1128\/IAI.00207-16","article-title":"The <italic>Clostridium difficile<\/italic> Dlt Pathway Is Controlled by the Extracytoplasmic Function Sigma Factor sigmaV in Response to Lysozyme","volume":"84","author":"EC Woods","year":"2016","journal-title":"Infect Immun"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1128\/JB.00038-14","article-title":"Identification and characterization of a gene cluster required for proper rod shape, cell division, and pathogenesis in <italic>Clostridium difficile<\/italic>","volume":"196","author":"EM Ransom","year":"2014","journal-title":"J Bacteriol"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"2345","DOI":"10.1128\/IAI.01483-13","article-title":"<italic>Clostridium difficile<\/italic> extracytoplasmic function sigma factor sigmaV regulates lysozyme resistance and is necessary for pathogenesis in the hamster model of infection","volume":"82","author":"TD Ho","year":"2014","journal-title":"Infect Immun"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"e1005239","DOI":"10.1371\/journal.ppat.1005239","article-title":"Identification of a Novel Lipoprotein Regulator of <italic>Clostridium difficile<\/italic> Spore Germination","volume":"11","author":"KA Fimlaid","year":"2015","journal-title":"PLoS Pathog"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/1574-6968.12428","article-title":"Capsule loss or death: the position of mutations among capsule genes sways the destiny of <italic>Streptococcus suis<\/italic>","volume":"354","author":"N Lakkitjaroen","year":"2014","journal-title":"FEMS Microbiol Lett"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1111\/j.1574-6968.2010.02086.x","article-title":"Cell surface characteristics of nontypeable isolates of <italic>Streptococcus suis<\/italic>","volume":"311","author":"L Bonifait","year":"2010","journal-title":"FEMS Microbiol Lett"},{"key":"ref64","first-page":"65","article-title":"Pleiotropic effects of polysaccharide capsule loss on selected biological properties of <italic>Streptococcus suis<\/italic>","volume":"74","author":"S Tanabe","year":"2010","journal-title":"Can J Vet Res"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.chembiol.2011.03.009","article-title":"A possible oligosaccharide-conjugate vaccine candidate for <italic>Clostridium difficile<\/italic> is antigenic and immunogenic","volume":"18","author":"MA Oberli","year":"2011","journal-title":"Chem Biol"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1586\/erv.13.9","article-title":"Carbohydrate-based <italic>Clostridium difficile<\/italic> vaccines","volume":"12","author":"MA Monteiro","year":"2013","journal-title":"Expert Rev Vaccines"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1005946","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T17:27:03Z","timestamp":1601141223000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1005946"}},"subtitle":[],"editor":[{"given":"Theresa M.","family":"Koehler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,10,14]]},"references-count":66,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2016,10,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1005946","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,14]]}}}