{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:52:39Z","timestamp":1789980759852,"version":"4.0.1"},"reference-count":69,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI078962"],"award-info":[{"award-number":["AI078962"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI034432"],"award-info":[{"award-number":["AI034432"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM007062"],"award-info":[{"award-number":["GM007062"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["I-1257"],"award-info":[{"award-number":["I-1257"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["I-1686"],"award-info":[{"award-number":["I-1686"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia (PT)","award":["SFRH\/BD\/51286\/2010"],"award-info":[{"award-number":["SFRH\/BD\/51286\/2010"]}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1006010","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T18:27:37Z","timestamp":1478543257000},"page":"e1006010","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":33,"title":["Pyrimidine Salvage Enzymes Are Essential for De Novo Biosynthesis of Deoxypyrimidine Nucleotides in Trypanosoma brucei"],"prefix":"10.1371","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0041-2502","authenticated-orcid":true,"given":"Christopher","family":"Leija","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipa","family":"Rijo-Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa N.","family":"Kinch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick V.","family":"Grishin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5209-1958","authenticated-orcid":true,"given":"Nicole","family":"Nischan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5373-3329","authenticated-orcid":true,"given":"Jennifer J.","family":"Kohler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret A.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2016,11,7]]},"reference":[{"issue":"4","key":"ref1","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1172\/JCI33945","article-title":"Kinetoplastids: related protozoan pathogens, different diseases","volume":"118","author":"K Stuart","year":"2008","journal-title":"J Clin Invest"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1093\/bmb\/lds031","article-title":"Management of trypanosomiasis and leishmaniasis","volume":"104","author":"MP Barrett","year":"2012","journal-title":"Br Med Bull"},{"issue":"10","key":"ref3","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.2174\/156802611795429167","article-title":"State of the art in African trypanosome drug discovery","volume":"11","author":"RT Jacobs","year":"2011","journal-title":"Curr Top Med Chem"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S1474-4422(12)70296-X","article-title":"Clinical features, diagnosis, and treatment of human African trypanosomiasis (sleeping sickness)","volume":"12","author":"PG Kennedy","year":"2013","journal-title":"Lancet neurology"},{"key":"ref5","article-title":"Trypanosomiasis, human African (sleeping sickness)","volume":"259","year":"2015","journal-title":"World Health Organization Fact sheet"},{"issue":"1","key":"ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0304-4165(82)90002-2","article-title":"UMP synthesis in the kinetoplastida","volume":"718","author":"DJ Hammond","year":"1982","journal-title":"Biochim Biophys Acta"},{"issue":"3","key":"ref7","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/0166-6851(84)90117-8","article-title":"Purine and pyrimidine metabolism in the Trypanosomatidae","volume":"13","author":"DJ Hammond","year":"1984","journal-title":"Mol Biochem Parasitol"},{"issue":"23","key":"ref8","doi-asserted-by":"crossref","first-page":"2456","DOI":"10.2174\/092986710791556023","article-title":"Inhibitors of the purine salvage pathway: a valuable approach for antiprotozoal chemotherapy?","volume":"17","author":"M Berg","year":"2010","journal-title":"Curr Med Chem"},{"issue":"5","key":"ref9","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1111\/mmi.13083","article-title":"GMP synthase is essential for viability and infectivity of Trypanosoma brucei despite a redundant purine salvage pathway","volume":"97","author":"Q Li","year":"2015","journal-title":"Mol Microbiol"},{"issue":"2","key":"ref10","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1124\/mol.112.082321","article-title":"Pyrimidine salvage in Trypanosoma brucei bloodstream forms and the trypanocidal action of halogenated pyrimidines","volume":"83","author":"JA Ali","year":"2013","journal-title":"Mol Pharmacol"},{"issue":"5","key":"ref11","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.femsre.2005.03.004","article-title":"Purine and pyrimidine transport in pathogenic protozoa: from biology to therapy","volume":"29","author":"HP de Koning","year":"2005","journal-title":"FEMS Microbiol Rev"},{"issue":"5733","key":"ref12","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1126\/science.1112181","article-title":"Comparative genomics of trypanosomatid parasitic protozoa","volume":"309","author":"NM El-Sayed","year":"2005","journal-title":"Science"},{"issue":"3","key":"ref13","doi-asserted-by":"crossref","first-page":"e58034","DOI":"10.1371\/journal.pone.0058034","article-title":"Pyrimidine biosynthesis is not an essential function for Trypanosoma brucei bloodstream forms","volume":"8","author":"JA Ali","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"ref14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1365-2958.2008.06131.x","article-title":"Characterization of Trypanosoma brucei dihydroorotate dehydrogenase as a possible drug target; structural, kinetic and RNAi studies","volume":"68","author":"TL Arakaki","year":"2008","journal-title":"Mol Microbiol"},{"issue":"2","key":"ref15","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/mmi.12376","article-title":"Trypanosoma brucei (UMP synthase null mutants) are avirulent in mice, but recover virulence upon prolonged culture in vitro while retaining pyrimidine auxotrophy","volume":"90","author":"HB Ong","year":"2013","journal-title":"Mol Microbiol"},{"issue":"12","key":"ref16","doi-asserted-by":"crossref","first-page":"11781","DOI":"10.1074\/jbc.M413821200","article-title":"Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei","volume":"280","author":"ML Ginger","year":"2005","journal-title":"J Biol Chem"},{"issue":"1","key":"ref17","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/S0021-9258(19)86279-8","article-title":"The modulation of the thymidine triphosphate pool of Chinese hamster cells by dCMP deaminase and UDP reductase. Thymidine auxotrophy induced by CTP in dCMP deaminase-deficient lines","volume":"255","author":"BR de Saint Vincent","year":"1980","journal-title":"J Biol Chem"},{"issue":"4","key":"ref18","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.1073\/pnas.78.4.2447","article-title":"Mutator phenotypes in mammalian cell mutants with distinct biochemical defects and abnormal deoxyribonucleoside triphosphate pools","volume":"78","author":"G Weinberg","year":"1981","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"ref19","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.molbiopara.2012.11.003","article-title":"Pyrimidine requirements in deoxyuridine triphosphate nucleotidohydrolase deficient Trypanosoma brucei mutants","volume":"187","author":"VM Castillo-Acosta","year":"2013","journal-title":"Mol Biochem Parasitol"},{"issue":"5","key":"ref20","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1016\/j.jmb.2010.01.013","article-title":"The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase","volume":"396","author":"ET Larson","year":"2010","journal-title":"J Mol Biol"},{"issue":"8","key":"ref21","doi-asserted-by":"crossref","first-page":"2722","DOI":"10.1016\/S0021-9258(19)44066-0","article-title":"A genetically distinct thymidine kinase in mammalian mitochondria. Exclusive labeling of mitochondrial deoxyribonucleic acid","volume":"248","author":"AJ Berk","year":"1973","journal-title":"J Biol Chem"},{"issue":"17","key":"ref22","doi-asserted-by":"crossref","first-page":"3686","DOI":"10.1021\/bi00662a007","article-title":"Human deoxythymidine kinase II: substrate specificity and kinetic behavior of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia","volume":"15","author":"LS Lee","year":"1976","journal-title":"Biochemistry"},{"issue":"2","key":"ref23","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/S0014-5793(98)01711-6","article-title":"Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates","volume":"443","author":"L Wang","year":"1999","journal-title":"FEBS Lett"},{"issue":"21","key":"ref24","doi-asserted-by":"crossref","first-page":"17628","DOI":"10.1074\/jbc.M112.340059","article-title":"Trypanosoma brucei thymidine kinase is tandem protein consisting of two homologous parts, which together enable efficient substrate binding","volume":"287","author":"F Ranjbarian","year":"2012","journal-title":"J Biol Chem"},{"key":"ref25","article-title":"Cell cycle regulation and novel structural features of thymidine kinase, an essential enzyme in Trypanosoma brucei","author":"M Valente","year":"2016","journal-title":"Mol Microbiol"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1101\/gr.115089.110","article-title":"High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome","volume":"21","author":"S Alsford","year":"2011","journal-title":"Genome Res"},{"issue":"5","key":"ref27","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1128\/EC.00015-14","article-title":"Genetic validation of Trypanosoma brucei glutathione synthetase as an essential enzyme","volume":"13","author":"C Pratt","year":"2014","journal-title":"Eukaryot Cell"},{"issue":"3","key":"ref28","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1046\/j.1365-2958.2000.01721.x","article-title":"Trypanosomes lacking trypanothione reductase are avirulent and show increased sensitivity to oxidative stress","volume":"35","author":"S Krieger","year":"2000","journal-title":"Mol Microbiol"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0166-6851(99)00002-X","article-title":"A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in T. brucei","volume":"99","author":"E Wirtz","year":"1999","journal-title":"Mol Biochem Parasitol"},{"issue":"45","key":"ref30","doi-asserted-by":"crossref","first-page":"31967","DOI":"10.1074\/jbc.274.45.31967","article-title":"Substrate diversity of herpes simplex virus thymidine kinase. Impact Of the kinematics of the enzyme","volume":"274","author":"BD Pilger","year":"1999","journal-title":"J Biol Chem"},{"issue":"3","key":"ref31","doi-asserted-by":"crossref","first-page":"308","DOI":"10.2174\/1574892810666150617112230","article-title":"Purine Analogues as Kinase Inhibitors: A Review","volume":"10","author":"S Sharma","year":"2015","journal-title":"Recent Pat Anticancer Drug Discov"},{"issue":"52","key":"ref32","doi-asserted-by":"crossref","first-page":"17970","DOI":"10.1073\/pnas.0406332102","article-title":"Structures of thymidine kinase 1 of human and mycoplasmic origin","volume":"101","author":"M Welin","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"3","key":"ref33","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1006\/jmbi.2000.3876","article-title":"Substrate binding is a prerequisite for stabilisation of mouse thymidine kinase in proliferating fibroblasts","volume":"300","author":"M Posch","year":"2000","journal-title":"J Mol Biol"},{"issue":"1","key":"ref34","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.neuron.2008.08.024","article-title":"Prostatic acid phosphatase is an ectonucleotidase and suppresses pain by generating adenosine","volume":"60","author":"MJ Zylka","year":"2008","journal-title":"Neuron"},{"issue":"12","key":"ref35","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1016\/j.str.2012.10.001","article-title":"Crystal structure of the human ecto-5&apos;-nucleotidase (CD73): insights into the regulation of purinergic signaling","volume":"20","author":"K Knapp","year":"2012","journal-title":"Structure"},{"issue":"1","key":"ref36","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.jmb.2008.02.036","article-title":"Structural insight into the mechanism of substrate specificity and catalytic activity of an HD-domain phosphohydrolase: the 5&apos;-deoxyribonucleotidase YfbR from Escherichia coli","volume":"378","author":"MD Zimmerman","year":"2008","journal-title":"J Mol Biol"},{"issue":"30","key":"ref37","doi-asserted-by":"crossref","first-page":"18678","DOI":"10.1074\/jbc.M115.657916","article-title":"Functional Diversity of Haloacid Dehalogenase Superfamily Phosphatases from Saccharomyces cerevisiae: BIOCHEMICAL, STRUCTURAL, AND EVOLUTIONARY INSIGHTS","volume":"290","author":"E Kuznetsova","year":"2015","journal-title":"J Biol Chem"},{"issue":"52","key":"ref38","doi-asserted-by":"crossref","first-page":"54687","DOI":"10.1074\/jbc.M411023200","article-title":"General enzymatic screens identify three new nucleotidases in Escherichia coli. Biochemical characterization of SurE, YfbR, and YjjG","volume":"279","author":"M Proudfoot","year":"2004","journal-title":"J Biol Chem"},{"issue":"21","key":"ref39","doi-asserted-by":"crossref","first-page":"4445","DOI":"10.1128\/MCB.01062-12","article-title":"Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant","volume":"32","author":"A Sanchez","year":"2012","journal-title":"Mol Cell Biol"},{"issue":"3","key":"ref40","doi-asserted-by":"crossref","first-page":"e1004689","DOI":"10.1371\/journal.ppat.1004689","article-title":"Probing the metabolic network in bloodstream-form Trypanosoma brucei using untargeted metabolomics with stable isotope labelled glucose","volume":"11","author":"DJ Creek","year":"2015","journal-title":"PLoS Pathog"},{"issue":"47","key":"ref41","doi-asserted-by":"crossref","first-page":"46195","DOI":"10.1074\/jbc.R300032200","article-title":"Mammalian 5&apos;-nucleotidases","volume":"278","author":"V Bianchi","year":"2003","journal-title":"J Biol Chem"},{"issue":"34","key":"ref42","doi-asserted-by":"crossref","first-page":"4249","DOI":"10.2174\/0929867311320340005","article-title":"The role of soluble 5&apos;-nucleotidases in the conversion of nucleotide analogs: metabolic and therapeutic aspects","volume":"20","author":"AC Skladanowski","year":"2013","journal-title":"Curr Med Chem"},{"issue":"1","key":"ref43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pharmthera.2005.01.003","article-title":"The 5&apos;-nucleotidases as regulators of nucleotide and drug metabolism","volume":"107","author":"SA Hunsucker","year":"2005","journal-title":"Pharmacol Ther"},{"issue":"4","key":"ref44","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1111\/mmi.12801","article-title":"The redundant aminotransferases in lysine and arginine synthesis and the extent of aminotransferase redundancy in Escherichia coli","volume":"94","author":"PB Lal","year":"2014","journal-title":"Mol Microbiol"},{"issue":"2","key":"ref45","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.molbiopara.2007.12.005","article-title":"Involvement of a Leishmania thymidine kinase in flagellum formation, promastigote shape and growth as well as virulence","volume":"158","author":"M Thiel","year":"2008","journal-title":"Mol Biochem Parasitol"},{"issue":"7561","key":"ref46","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1038\/nature14651","article-title":"Genetic modification of the diarrhoeal pathogen Cryptosporidium parvum","volume":"523","author":"S Vinayak","year":"2015","journal-title":"Nature"},{"issue":"35","key":"ref47","doi-asserted-by":"crossref","first-page":"27327","DOI":"10.1074\/jbc.M110.137042","article-title":"Regulation and functional contribution of thymidine kinase 1 in repair of DNA damage","volume":"285","author":"YL Chen","year":"2010","journal-title":"J Biol Chem"},{"issue":"51","key":"ref48","doi-asserted-by":"crossref","first-page":"40174","DOI":"10.1074\/jbc.M008405200","article-title":"Inhibition of Trypanosoma brucei gene expression by RNA interference using an integratable vector with opposing T7 promoters","volume":"275","author":"Z Wang","year":"2000","journal-title":"J Biol Chem"},{"issue":"21","key":"ref49","doi-asserted-by":"crossref","first-page":"15256","DOI":"10.1074\/jbc.M113.461137","article-title":"Allosteric activation of trypanosomatid deoxyhypusine synthase by a catalytically dead paralog","volume":"288","author":"S Nguyen","year":"2013","journal-title":"J Biol Chem"},{"issue":"1","key":"ref50","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.molbiopara.2013.05.002","article-title":"Identification of essential and non-essential protein kinases by a fusion PCR method for efficient production of transgenic Trypanosoma brucei","volume":"190","author":"C Merritt","year":"2013","journal-title":"Mol Biochem Parasitol"},{"issue":"1\u20132","key":"ref51","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.molbiopara.2011.03.002","article-title":"A Gateway(R) compatible vector for gene silencing in bloodstream form Trypanosoma brucei","volume":"178","author":"S Kalidas","year":"2011","journal-title":"Mol Biochem Parasitol"},{"issue":"5","key":"ref52","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1016\/j.celrep.2014.04.037","article-title":"Oxidation of alpha-ketoglutarate is required for reductive carboxylation in cancer cells with mitochondrial defects","volume":"7","author":"AR Mullen","year":"2014","journal-title":"Cell Rep"},{"issue":"43","key":"ref53","doi-asserted-by":"crossref","first-page":"16886","DOI":"10.1073\/pnas.0708365104","article-title":"Cyclic changes in metabolic state during the life of a yeast cell","volume":"104","author":"BP Tu","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"ref54","doi-asserted-by":"crossref","first-page":"e85","DOI":"10.1093\/nar\/gkp1141","article-title":"Quantitation of cellular deoxynucleoside triphosphates","volume":"38","author":"P Ferraro","year":"2010","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"ref55","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/0003-2697(89)90420-X","article-title":"Enzymatic assay for deoxyribonucleoside triphosphates using synthetic oligonucleotides as template primers","volume":"180","author":"PA Sherman","year":"1989","journal-title":"Anal Biochem"},{"issue":"1","key":"ref56","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1006\/abio.2001.5091","article-title":"Determination of nucleotides and sugar nucleotides involved in protein glycosylation by high-performance anion-exchange chromatography: sugar nucleotide contents in cultured insect cells and mammalian cells","volume":"293","author":"N Tomiya","year":"2001","journal-title":"Anal Biochem"},{"issue":"1","key":"ref57","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.molbiopara.2010.03.007","article-title":"Selection of reference genes for mRNA quantification in Trypanosoma brucei","volume":"172","author":"M Brenndorfer","year":"2010","journal-title":"Molecular and biochemical parasitology"},{"issue":"9","key":"ref58","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"MW Pfaffl","year":"2001","journal-title":"Nucleic acids research"},{"issue":"6","key":"ref59","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.chom.2016.05.002","article-title":"Trypanosoma brucei Parasites Occupy and Functionally Adapt to the Adipose Tissue in Mice","volume":"19","author":"S Trindade","year":"2016","journal-title":"Cell Host Microbe"},{"issue":"D1","key":"ref60","doi-asserted-by":"crossref","first-page":"D457","DOI":"10.1093\/nar\/gkv1070","article-title":"KEGG as a reference resource for gene and protein annotation","volume":"44","author":"M Kanehisa","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"ref61","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: kyoto encyclopedia of genes and genomes","volume":"28","author":"M Kanehisa","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"D204","DOI":"10.1093\/nar\/gku989","article-title":"UniProt: a hub for protein information","volume":"43","author":"C UniProt","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"D1","key":"ref63","doi-asserted-by":"crossref","first-page":"D279","DOI":"10.1093\/nar\/gkv1344","article-title":"The Pfam protein families database: towards a more sustainable future","volume":"44","author":"RD Finn","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"17","key":"ref64","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"SF Altschul","year":"1997","journal-title":"Nucleic Acids Res"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"D222","DOI":"10.1093\/nar\/gku1221","article-title":"CDD: NCBI&apos;s conserved domain database","volume":"43","author":"A Marchler-Bauer","year":"2015","journal-title":"Nucleic Acids Res"},{"issue":"Suppl 9","key":"ref66","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1002\/prot.22499","article-title":"Fast and accurate automatic structure prediction with HHpred","volume":"77","author":"A Hildebrand","year":"2009","journal-title":"Proteins"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gki408","article-title":"The HHpred interactive server for protein homology detection and structure prediction","volume":"33","author":"J Soding","year":"2005","journal-title":"Nucleic Acids Res"},{"issue":"7","key":"ref68","doi-asserted-by":"crossref","first-page":"5232","DOI":"10.1074\/jbc.M112.442475","article-title":"Trypanosoma brucei S-adenosylmethionine decarboxylase N terminus is essential for allosteric activation by the regulatory subunit prozyme","volume":"288","author":"N Velez","year":"2013","journal-title":"J Biol Chem"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/S0076-6879(05)04043-7","article-title":"Robust colorimetric assays for dynamin&apos;s basal and stimulated GTPase activities","volume":"404","author":"M Leonard","year":"2005","journal-title":"Methods Enzymol"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1006010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T17:30:33Z","timestamp":1657647033000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1006010"}},"subtitle":[],"editor":[{"given":"David","family":"Horn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,11,7]]},"references-count":69,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2016,11,7]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1006010","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,7]]}}}