{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:15:19Z","timestamp":1791296119272,"version":"4.1.0"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1006180","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T18:25:25Z","timestamp":1486578325000},"page":"e1006180","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":50,"title":["The Malaria Parasite's Lactate Transporter PfFNT Is the Target of Antiplasmodial Compounds Identified in Whole Cell Phenotypic Screens"],"prefix":"10.1371","volume":"13","author":[{"given":"Sanduni V.","family":"Hapuarachchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon A.","family":"Cobbold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6503-3565","authenticated-orcid":true,"given":"Sarah H.","family":"Shafik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5473-629X","authenticated-orcid":true,"given":"Adelaide S. M.","family":"Dennis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm J.","family":"McConville","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rowena E.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5613-2622","authenticated-orcid":true,"given":"Kiaran","family":"Kirk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adele M.","family":"Lehane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"ref1","unstructured":"WHO. World Malaria Report. 2015."},{"key":"ref2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.4269\/ajtmh.15-0007","article-title":"Antimalarial drug resistance: Literature review and activities and findings of the ICEMR network","volume":"93","author":"L Cui","year":"2015","journal-title":"Am J Trop Med Hyg"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1038\/nrd4573","article-title":"Malaria medicines: a glass half full?","volume":"14","author":"TN Wells","year":"2015","journal-title":"Nat Rev Drug Discov"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"e1000402","DOI":"10.1371\/journal.pmed.1000402","article-title":"A research agenda for malaria eradication: drugs","volume":"8","year":"2011","journal-title":"PLoS Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial lead identification","volume":"465","author":"FJ Gamo","year":"2010","journal-title":"Nature"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nature09099","article-title":"Chemical genetics of <italic>Plasmodium falciparum<\/italic>","volume":"465","author":"WA Guiguemde","year":"2010","journal-title":"Nature"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"9059","DOI":"10.1073\/pnas.0802982105","article-title":"In silico activity profiling reveals the mechanism of action of antimalarials discovered in a high-throughput screen","volume":"105","author":"D Plouffe","year":"2008","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"e62906","DOI":"10.1371\/journal.pone.0062906","article-title":"The open access malaria box: a drug discovery catalyst for neglected diseases","volume":"8","author":"T Spangenberg","year":"2013","journal-title":"PLoS One"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/1741-7007-11-67","article-title":"Mitochondrial metabolism of sexual and asexual blood stages of the malaria parasite <italic>Plasmodium falciparum<\/italic>","volume":"11","author":"JI MacRae","year":"2013","journal-title":"BMC Biol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"6151","DOI":"10.1016\/j.febslet.2005.09.088","article-title":"Malaria parasite-infected erythrocytes inhibit glucose utilization in uninfected red cells","volume":"579","author":"M Mehta","year":"2005","journal-title":"FEBS Lett"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1042\/bj3550733","article-title":"Transport of lactate and pyruvate in the intraerythrocytic malaria parasite, <italic>Plasmodium falciparum<\/italic>","volume":"355","author":"JL Elliott","year":"2001","journal-title":"Biochem J"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"7476","DOI":"10.1073\/pnas.1330865100","article-title":"Validation of the hexose transporter of <italic>Plasmodium falciparum<\/italic> as a novel drug target","volume":"100","author":"T Joet","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.tmaid.2008.01.005","article-title":"New antimalarial targets: the example of glucose transport","volume":"6","author":"AP Patel","year":"2008","journal-title":"Travel Med Infect Dis"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"e0123598","DOI":"10.1371\/journal.pone.0123598","article-title":"Identification of selective inhibitors of the <italic>Plasmodium falciparum<\/italic> Hexose Transporter PfHT by screening focused libraries of anti-malarial compounds","volume":"10","author":"D Ortiz","year":"2015","journal-title":"PLoS One"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.pep.2016.06.008","article-title":"High-level cell-free production of the malarial lactate transporter PfFNT as a basis for crystallization trials and directional transport studies","volume":"126","author":"J Holm-Bertelsen","year":"2016","journal-title":"Protein Expr Purif"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"6721","DOI":"10.1038\/ncomms7721","article-title":"A lactate and formate transporter in the intraerythrocytic malaria parasite, <italic>Plasmodium falciparum<\/italic>","volume":"6","author":"RV Marchetti","year":"2015","journal-title":"Nat Commun"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"6284","DOI":"10.1038\/ncomms7284","article-title":"Identity of a <italic>Plasmodium<\/italic> lactate\/H<sup>+<\/sup> symporter structurally unrelated to human transporters","volume":"6","author":"B Wu","year":"2015","journal-title":"Nat Commun"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1111\/mmi.12765","article-title":"Diverse chemotypes disrupt ion homeostasis in the malaria parasite","volume":"94","author":"AM Lehane","year":"2014","journal-title":"Mol Microbiol"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.chom.2012.12.006","article-title":"Na<sup>+<\/sup> regulation in the malaria parasite <italic>Plasmodium falciparum<\/italic> involves the cation ATPase PfATP4 and is a target of the spiroindolone antimalarials","volume":"13","author":"NJ Spillman","year":"2013","journal-title":"Cell Host Microbe"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"1291","DOI":"10.1016\/j.bcp.2009.12.025","article-title":"Inhibition of <italic>Plasmodium falciparum<\/italic> pH regulation by small molecule indole derivatives results in rapid parasite death","volume":"79","author":"DA van Schalkwyk","year":"2010","journal-title":"Biochem Pharmacol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1242\/jcs.02795","article-title":"The pH of the digestive vacuole of <italic>Plasmodium falciparum<\/italic> is not associated with chloroquine resistance","volume":"119","author":"R Hayward","year":"2006","journal-title":"J Cell Sci"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1042\/BJ20070934","article-title":"Evaluation of pH during cytostomal endocytosis and vacuolar catabolism of haemoglobin in <italic>Plasmodium falciparum<\/italic>","volume":"407","author":"N Klonis","year":"2007","journal-title":"Biochem J"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1111\/j.1462-5822.2006.00847.x","article-title":"Quantitative pH measurements in <italic>Plasmodium falciparum<\/italic>-infected erythrocytes using pHluorin","volume":"9","author":"Y Kuhn","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"11264","DOI":"10.1074\/jbc.M311110200","article-title":"The membrane potential of the intraerythrocytic malaria parasite <italic>Plasmodium falciparum<\/italic>","volume":"279","author":"RJ Allen","year":"2004","journal-title":"J Biol Chem"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"33213","DOI":"10.1074\/jbc.274.47.33213","article-title":"pH regulation in the intracellular malaria parasite, <italic>Plasmodium falciparum<\/italic>. H<sup>+<\/sup> extrusion via a V-type H<sup>+<\/sup>-ATPase","volume":"274","author":"KJ Saliba","year":"1999","journal-title":"J Biol Chem"},{"key":"ref26","doi-asserted-by":"crossref","first-page":"1680","DOI":"10.1126\/science.1175667","article-title":"Chloroquine transport via the malaria parasite&apos;s chloroquine resistance transporter","volume":"325","author":"RE Martin","year":"2009","journal-title":"Science"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"10683","DOI":"10.1074\/jbc.275.14.10683","article-title":"Isolation and functional characterization of the PfNT1 nucleoside transporter gene from <italic>Plasmodium falciparum<\/italic>","volume":"275","author":"NS Carter","year":"2000","journal-title":"J Biol Chem"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1042\/bj3490067","article-title":"Identification of a nucleoside\/nucleobase transporter from <italic>Plasmodium falciparum<\/italic>, a novel target for anti-malarial chemotherapy","volume":"349","author":"MD Parker","year":"2000","journal-title":"Biochem J"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1093\/infdis\/jiv372","article-title":"Metabolic dysregulation induced in <italic>Plasmodium falciparum<\/italic> by dihydroartemisinin and other front-line antimalarial drugs","volume":"213","author":"SA Cobbold","year":"2016","journal-title":"J Infect Dis"},{"key":"ref30","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/nature14451","article-title":"A novel multiple-stage antimalarial agent that inhibits protein synthesis","volume":"522","author":"B Baragana","year":"2015","journal-title":"Nature"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"E5455","DOI":"10.1073\/pnas.1414221111","article-title":"(+)-SJ733, a clinical candidate for malaria that acts through ATP4 to induce rapid host-mediated clearance of <italic>Plasmodium<\/italic>","volume":"111","author":"MB Jimenez-Diaz","year":"2014","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1126\/science.1193225","article-title":"Spiroindolones, a potent compound class for the treatment of malaria","volume":"329","author":"M Rottmann","year":"2010","journal-title":"Science"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"5521","DOI":"10.1038\/ncomms6521","article-title":"Pyrazoleamide compounds are potent antimalarials that target Na<sup>+<\/sup> homeostasis in intraerythrocytic <italic>Plasmodium falciparum<\/italic>","volume":"5","author":"AB Vaidya","year":"2014","journal-title":"Nat Commun"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"11901","DOI":"10.1038\/ncomms11901","article-title":"A broad analysis of resistance development in the malaria parasite","volume":"7","author":"VC Corey","year":"2016","journal-title":"Nat Commun"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1186\/1475-2875-11-292","article-title":"A framework for assessing the risk of resistance for anti-malarials in development","volume":"11","author":"XC Ding","year":"2012","journal-title":"Malar J"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"e1005763","DOI":"10.1371\/journal.ppat.1005763","article-title":"Open source drug discovery with the Malaria Box compound collection for neglected diseases and beyond","volume":"12","author":"WC Van Voorhis","year":"2016","journal-title":"PLoS Pathog"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.ijpddr.2015.07.001","article-title":"The malaria parasite cation ATPase PfATP4 and its role in the mechanism of action of a new arsenal of antimalarial drugs","volume":"5","author":"NJ Spillman","year":"2015","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/978-1-60761-652-8_6","article-title":"A method for rapid genetic integration into <italic>Plasmodium falciparum<\/italic> utilizing mycobacteriophage Bxb1 integrase","volume":"634","author":"SH Adjalley","year":"2010","journal-title":"Methods Mol Biol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1126\/science.781840","article-title":"Human malaria parasites in continuous culture","volume":"193","author":"W Trager","year":"1976","journal-title":"Science"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.molbiopara.2009.09.005","article-title":"<italic>Plasmodium falciparum<\/italic> culture: the benefits of shaking","volume":"169","author":"RJ Allen","year":"2010","journal-title":"Mol Biochem Parasitol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2307\/3280287","article-title":"Synchronization of <italic>Plasmodium falciparum<\/italic> erythrocytic stages in culture","volume":"65","author":"C Lambros","year":"1979","journal-title":"J Parasitol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"10190","DOI":"10.1074\/jbc.273.17.10190","article-title":"Transport and metabolism of the essential vitamin pantothenic acid in human erythrocytes infected with the malaria parasite <italic>Plasmodium falciparum<\/italic>","volume":"273","author":"KJ Saliba","year":"1998","journal-title":"J Biol Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1111\/j.1365-2958.2010.07272.x","article-title":"Efflux of a range of antimalarial drugs and &apos;chloroquine resistance reversers&apos; from the digestive vacuole in malaria parasites with mutant PfCRT","volume":"77","author":"AM Lehane","year":"2010","journal-title":"Mol Microbiol"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.1242\/jcs.016758","article-title":"A verapamil-sensitive chloroquine-associated H<sup>+<\/sup> leak from the digestive vacuole in chloroquine-resistant malaria parasites","volume":"121","author":"AM Lehane","year":"2008","journal-title":"J Cell Sci"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1128\/AAC.48.5.1803-1806.2004","article-title":"Simple and inexpensive fluorescence-based technique for high-throughput antimalarial drug screening","volume":"48","author":"M Smilkstein","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"e54974","DOI":"10.1371\/journal.pone.0054974","article-title":"Pantothenamides are potent, on-target inhibitors of <italic>Plasmodium falciparum<\/italic> growth when serum pantetheinase is inactivated","volume":"8","author":"C Spry","year":"2013","journal-title":"PLoS One"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/S0140-6736(81)90414-1","article-title":"Separation of malaria-infected erythrocytes from whole blood: use of a selective high-gradient magnetic separation technique","volume":"2","author":"F Paul","year":"1981","journal-title":"Lancet"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1002\/(SICI)1097-0320(19990401)35:4<329::AID-CYTO5>3.0.CO;2-Y","article-title":"Detection of antibodies to variant antigens on <italic>Plasmodium falciparum<\/italic>-infected erythrocytes by flow cytometry","volume":"35","author":"T Staalsoe","year":"1999","journal-title":"Cytometry"},{"key":"ref49","doi-asserted-by":"crossref","DOI":"10.1002\/0471250953.bi1411s37","article-title":"LC-MS data processing with MAVEN: a metabolomic analysis and visualization engine","volume":"14","author":"MF Clasquin","year":"2012","journal-title":"Curr Protoc Bioinformatics"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1038\/nature05149","article-title":"Sodium-dependent uptake of inorganic phosphate by the intracellular malaria parasite","volume":"443","author":"KJ Saliba","year":"2006","journal-title":"Nature"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1093\/infdis\/jiv509","article-title":"Verapamil-sensitive transport of quinacrine and methylene blue via the <italic>Plasmodium falciparum<\/italic> Chloroquine Resistance Transporter reduces the parasite&apos;s susceptibility to these tricyclic drugs","volume":"213","author":"DA van Schalkwyk","year":"2016","journal-title":"J Infect Dis"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"e1005725","DOI":"10.1371\/journal.ppat.1005725","article-title":"Molecular mechanisms for drug hypersensitivity induced by the malaria parasite&apos;s Chloroquine Resistance Transporter","volume":"12","author":"SN Richards","year":"2016","journal-title":"PLoS Pathogens"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"W251","DOI":"10.1093\/nar\/gkv380","article-title":"MetaboAnalyst 3.0\u2014making metabolomics more meaningful","volume":"43","author":"J Xia","year":"2015","journal-title":"Nucleic Acids Res"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1111\/j.1365-2958.2006.05125.x","article-title":"Transport of nucleosides across the <italic>Plasmodium falciparum<\/italic> parasite plasma membrane has characteristics of PfENT1","volume":"60","author":"MJ Downie","year":"2006","journal-title":"Mol Microbiol"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1006180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T10:58:04Z","timestamp":1719053884000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1006180"}},"subtitle":[],"editor":[{"given":"Margaret A.","family":"Phillips","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,2,8]]},"references-count":54,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2,8]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1006180","relation":{},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,8]]}}}