{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:38:34Z","timestamp":1791275914858,"version":"4.1.0"},"update-to":[{"DOI":"10.1371\/journal.ppat.1006221","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000}}],"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T00:00:00Z","timestamp":1487635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["543107"],"award-info":[{"award-number":["543107"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1121697"],"award-info":[{"award-number":["1121697"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["NIH RO1 GM064347"],"award-info":[{"award-number":["NIH RO1 GM064347"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1006221","type":"journal-article","created":{"date-parts":[[2017,2,21]],"date-time":"2017-02-21T19:27:13Z","timestamp":1487705233000},"page":"e1006221","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":50,"title":["The thermodynamics of Pr55Gag-RNA interaction regulate the assembly of HIV"],"prefix":"10.1371","volume":"13","author":[{"given":"Hanumant S.","family":"Tanwar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith K.","family":"Khoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7988-7334","authenticated-orcid":true,"given":"Megan","family":"Garvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynne","family":"Waddington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4905-9401","authenticated-orcid":true,"given":"Andrew","family":"Leis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcel","family":"Hijnen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Velkov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5080-8791","authenticated-orcid":true,"given":"Geoff J.","family":"Dumsday","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"McKinstry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5229-5707","authenticated-orcid":true,"given":"Johnson","family":"Mak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,2,21]]},"reference":[{"issue":"3","key":"ref1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.tim.2012.11.006","article-title":"HIV Gag polyprotein: processing and early viral particle assembly","volume":"21","author":"NM Bell","year":"2013","journal-title":"Trends in microbiology"},{"issue":"8","key":"ref2","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/nrmicro3490","article-title":"HIV-1 assembly, release and maturation","volume":"13","author":"EO Freed","year":"2015","journal-title":"Nat Rev Microbiol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/978-1-4614-0980-9_20","article-title":"Assembly and architecture of HIV","volume":"726","author":"BK Ganser-Pornillos","year":"2012","journal-title":"Advances in experimental medicine and biology"},{"issue":"2","key":"ref4","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.virusres.2012.10.016","article-title":"Elements in HIV-1 Gag contributing to virus particle assembly","volume":"171","author":"IP O'Carroll","year":"2013","journal-title":"Virus Res"},{"issue":"9","key":"ref5","doi-asserted-by":"crossref","first-page":"5246","DOI":"10.1073\/pnas.091000398","article-title":"RNA is a structural element in retrovirus particles","volume":"98","author":"D Muriaux","year":"2001","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"ref6","first-page":"373","article-title":"Diverse interactions of retroviral Gag proteins with RNAs","volume":"36","author":"A Rein","year":"2011","journal-title":"Trends in biochemical sciences"},{"issue":"10","key":"ref7","doi-asserted-by":"crossref","first-page":"6487","DOI":"10.1128\/JVI.69.10.6487-6497.1995","article-title":"Self-assembly in vitro of purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1","volume":"69","author":"S Campbell","year":"1995","journal-title":"Journal of virology"},{"issue":"3","key":"ref8","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1128\/JVI.73.3.2270-2279.1999","article-title":"In vitro assembly properties of human immunodeficiency virus type 1 Gag protein lacking the p6 domain","volume":"73","author":"S Campbell","year":"1999","journal-title":"Journal of virology"},{"issue":"3","key":"ref9","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1128\/JVI.72.3.1902-1909.1998","article-title":"Sequence-specific binding of human immunodeficiency virus type 1 nucleocapsid protein to short oligonucleotides","volume":"72","author":"RJ Fisher","year":"1998","journal-title":"Journal of virology"},{"issue":"6","key":"ref10","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1016\/j.jmb.2015.01.015","article-title":"Role of the nucleocapsid domain in HIV-1 Gag oligomerization and trafficking to the plasma membrane: a fluorescence lifetime imaging microscopy investigation","volume":"427","author":"SE El Meshri","year":"2015","journal-title":"Journal of molecular biology"},{"issue":"4","key":"ref11","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1083\/jcb.201504006","article-title":"Live-cell observation of cytosolic HIV-1 assembly onset reveals RNA-interacting Gag oligomers","volume":"210","author":"J Hendrix","year":"2015","journal-title":"The Journal of cell biology"},{"issue":"45","key":"ref12","doi-asserted-by":"crossref","first-page":"19114","DOI":"10.1073\/pnas.0907364106","article-title":"Imaging the interaction of HIV-1 genomes and Gag during assembly of individual viral particles","volume":"106","author":"N Jouvenet","year":"2009","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"11","key":"ref13","doi-asserted-by":"crossref","first-page":"e1001200","DOI":"10.1371\/journal.ppat.1001200","article-title":"Analysis of the initiating events in HIV-1 particle assembly and genome packaging","volume":"6","author":"SB Kutluay","year":"2010","journal-title":"PLoS Pathog"},{"issue":"48","key":"ref14","doi-asserted-by":"crossref","first-page":"E5205","DOI":"10.1073\/pnas.1413169111","article-title":"Cytoplasmic HIV-1 RNA is mainly transported by diffusion in the presence or absence of Gag protein","volume":"111","author":"J Chen","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1038\/srep39332","article-title":"Self-assembly of HIV-1 Gag protein on lipid membranes generates PI(4,5)P2\/Cholesterol nanoclusters","volume":"6","author":"N Yandrapalli","year":"2016","journal-title":"Scientific Reports"},{"issue":"2","key":"ref16","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1093\/nar\/gkj458","article-title":"Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins","volume":"34","author":"M Cruceanu","year":"2006","journal-title":"Nucleic Acids Res"},{"issue":"16","key":"ref17","doi-asserted-by":"crossref","first-page":"5276","DOI":"10.1021\/bi016045+","article-title":"Affinities of packaging domain loops in HIV-1 RNA for the nucleocapsid protein","volume":"41","author":"MF Shubsda","year":"2002","journal-title":"Biochemistry"},{"issue":"8","key":"ref18","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1261\/rna.038869.113","article-title":"Distinct binding interactions of HIV-1 Gag to Psi and non-Psi RNAs: implications for viral genomic RNA packaging","volume":"19","author":"JA Webb","year":"2013","journal-title":"Rna"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"4304","DOI":"10.1038\/ncomms5304","article-title":"Specific recognition of the HIV-1 genomic RNA by the Gag precursor","volume":"5","author":"EW Abd El-Wahab","year":"2014","journal-title":"Nat Commun"},{"issue":"1","key":"ref20","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1080\/15476286.2016.1256533","article-title":"HIV-1 Pr55Gag binds genomic and spliced RNAs with different affinity and stoichiometry","volume":"14","author":"S Bernacchi","year":"2017","journal-title":"RNA Biology"},{"issue":"5","key":"ref21","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/j.cell.2014.09.057","article-title":"Global changes in the RNA binding specificity of HIV-1 gag regulate virion genesis","volume":"159","author":"SB Kutluay","year":"2014","journal-title":"Cell"},{"issue":"42","key":"ref22","doi-asserted-by":"crossref","first-page":"16928","DOI":"10.1073\/pnas.1211759109","article-title":"In vitro reconstitution of the ordered assembly of the endosomal sorting complex required for transport at membrane-bound HIV-1 Gag clusters","volume":"109","author":"LA Carlson","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"19","key":"ref23","doi-asserted-by":"crossref","first-page":"10875","DOI":"10.1073\/pnas.191224698","article-title":"Modulation of HIV-like particle assembly in vitro by inositol phosphates","volume":"98","author":"S Campbell","year":"2001","journal-title":"Proceedings Of The National Academy Of Sciences Of The United States Of America"},{"issue":"2","key":"ref24","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.jmb.2010.11.051","article-title":"HIV-1 Gag extension: conformational changes require simultaneous interaction with membrane and nucleic acid","volume":"406","author":"SA Datta","year":"2011","journal-title":"Journal of molecular biology"},{"issue":"3","key":"ref25","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.jmb.2006.10.072","article-title":"Interactions between HIV-1 Gag molecules in solution: an inositol phosphate-mediated switch","volume":"365","author":"SA Datta","year":"2007","journal-title":"J Mol Biol"},{"issue":"6","key":"ref26","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.1128\/JVI.03353-13","article-title":"A conformational transition observed in single HIV-1 Gag molecules during in vitro assembly of virus-like particles","volume":"88","author":"JB Munro","year":"2014","journal-title":"Journal of virology"},{"issue":"5349","key":"ref27","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1126\/science.279.5349.384","article-title":"Structure of the HIV-1 nucleocapsid protein bound to the SL3 psi-RNA recognition element","volume":"279","author":"RN De Guzman","year":"1998","journal-title":"Science"},{"issue":"9","key":"ref28","doi-asserted-by":"crossref","first-page":"5439","DOI":"10.1128\/JVI.77.9.5439-5450.2003","article-title":"Functional surfaces of the human immunodeficiency virus type 1 capsid protein","volume":"77","author":"UK von Schwedler","year":"2003","journal-title":"J Virol"},{"key":"ref29","article-title":"Isothermal titration calorimetry","author":"A Velazquez-Campoy","year":"2004","journal-title":"Current protocols in cell biology \/ editorial board, Juan S Bonifacino [et al]"},{"issue":"2","key":"ref30","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1021\/mp800227w","article-title":"Thermodynamics of lipophilic drug binding to intestinal fatty acid binding protein and permeation across membranes","volume":"6","author":"T Velkov","year":"2009","journal-title":"Mol Pharm"},{"issue":"5","key":"ref31","doi-asserted-by":"crossref","first-page":"2545","DOI":"10.1128\/JVI.78.5.2545-2552.2004","article-title":"Assembly properties of the human immunodeficiency virus type 1 CA protein","volume":"78","author":"BK Ganser-Pornillos","year":"2004","journal-title":"J Virol"},{"issue":"7535","key":"ref32","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/nature13838","article-title":"Structure of the immature HIV-1 capsid in intact virus particles at 8.8 A resolution","volume":"517","author":"FK Schur","year":"2015","journal-title":"Nature"},{"issue":"11","key":"ref33","doi-asserted-by":"crossref","first-page":"7145","DOI":"10.1093\/nar\/gku335","article-title":"Distinct nucleic acid interaction properties of HIV-1 nucleocapsid protein precursor NCp15 explain reduced viral infectivity","volume":"42","author":"W Wang","year":"2014","journal-title":"Nucleic acids research"},{"issue":"2","key":"ref34","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1093\/nar\/gkj442","article-title":"Complex interactions of HIV-1 nucleocapsid protein with oligonucleotides","volume":"34","author":"RJ Fisher","year":"2006","journal-title":"Nucleic acids research"},{"issue":"19","key":"ref35","doi-asserted-by":"crossref","first-page":"3358","DOI":"10.1021\/bi400125z","article-title":"Probing the RNA binding surface of the HIV-1 nucleocapsid protein by site-directed mutagenesis","volume":"52","author":"W Ouyang","year":"2013","journal-title":"Biochemistry"},{"key":"ref36","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.14663","article-title":"Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies","volume":"5","author":"LA Carlson","year":"2016","journal-title":"eLife"},{"issue":"3","key":"ref37","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.1128\/JVI.73.3.2270-2279.1999","article-title":"In vitro Assembly Properties of Human Immunodeficiency Virus Type 1 Gag Protein Lacking the p6 Domain","volume":"73","author":"S Campbell","year":"1999","journal-title":"J Virol"},{"issue":"2","key":"ref38","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1089\/08892220252779674","article-title":"Codon and amino acid usage in retroviral genomes is consistent with virus-specific nucleotide pressure","volume":"18","author":"B Berkhout","year":"2002","journal-title":"AIDS Res Hum Retroviruses"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1186\/1742-4690-9-92","article-title":"The biased nucleotide composition of the HIV genome: a constant factor in a highly variable virus","volume":"9","author":"AC van der Kuyl","year":"2012","journal-title":"Retrovirology"},{"key":"ref40","first-page":"1","article-title":"The APOBEC3 family of retroelement restriction factors","volume":"371","author":"EW Refsland","year":"2013","journal-title":"Current topics in microbiology and immunology"},{"issue":"49","key":"ref41","doi-asserted-by":"crossref","first-page":"40875","DOI":"10.1074\/jbc.R112.416925","article-title":"The restriction factors of human immunodeficiency virus","volume":"287","author":"RS Harris","year":"2012","journal-title":"J Biol Chem"},{"issue":"10","key":"ref42","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.1128\/JVI.74.10.4839-4852.2000","article-title":"A Rev-independent human immunodeficiency virus type 1 (HIV-1)-based vector that exploits a codon-optimized HIV-1 gag-pol gene","volume":"74","author":"E Kotsopoulou","year":"2000","journal-title":"Journal of virology"},{"issue":"22","key":"ref43","doi-asserted-by":"crossref","first-page":"10822","DOI":"10.1128\/JVI.74.22.10822-10826.2000","article-title":"Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression","volume":"74","author":"M Graf","year":"2000","journal-title":"J Virol"},{"issue":"3","key":"ref44","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1093\/nar\/gkn1015","article-title":"The A-rich RNA sequences of HIV-1 pol are important for the synthesis of viral cDNA","volume":"37","author":"CP Keating","year":"2009","journal-title":"Nucleic Acids Res"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.pep.2014.04.013","article-title":"Expression and purification of soluble recombinant full length HIV-1 Pr55(Gag) protein in Escherichia coli","volume":"100","author":"WJ McKinstry","year":"2014","journal-title":"Protein Expr Purif"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.virol.2015.09.006","article-title":"Cryo-electron microscopy and single molecule fluorescent microscopy detect CD4 receptor induced HIV size expansion prior to cell entry","volume":"486","author":"S Pham","year":"2015","journal-title":"Virology"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"MM Bradford","year":"1976","journal-title":"Anal Biochem"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1006221","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1006221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T20:23:54Z","timestamp":1601670234000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1006221"}},"subtitle":[],"editor":[{"given":"Christopher","family":"Aiken","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,2,21]]},"references-count":47,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2,21]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1006221","relation":{"new_version":[{"id-type":"doi","id":"10.1371\/journal.ppat.1006221","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,21]]}}}