{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:28:49Z","timestamp":1790886529569,"version":"4.1.0"},"reference-count":81,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T00:00:00Z","timestamp":1501718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (BR)","award":["2013\/08216-2"],"award-info":[{"award-number":["2013\/08216-2"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo (FAPESP)","award":["2014\/04684-4"],"award-info":[{"award-number":["2014\/04684-4"]}]},{"name":"Conselho Nacional do Desenvolvimento Cientifico e Tecnol\u00f3gico (CNPq)"},{"name":"PEW Program In Biomedical Sciences"},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)"}],"content-domain":{"domain":["www.plospathogens.org"],"crossmark-restriction":false},"short-container-title":["PLoS Pathog"],"DOI":"10.1371\/journal.ppat.1006502","type":"journal-article","created":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T13:39:15Z","timestamp":1501767555000},"page":"e1006502","update-policy":"https:\/\/doi.org\/10.1371\/journal.ppat.corrections_policy","source":"Crossref","is-referenced-by-count":135,"title":["Inhibition of caspase-1 or gasdermin-D enable caspase-8 activation in the Naip5\/NLRC4\/ASC inflammasome"],"prefix":"10.1371","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2464-9416","authenticated-orcid":true,"given":"Danielle P. A.","family":"Mascarenhas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8051-3396","authenticated-orcid":true,"given":"Daiane M.","family":"Cerqueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo S. F.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2024-7858","authenticated-orcid":true,"given":"Fernanda V. S.","family":"Castanheira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4005-6039","authenticated-orcid":true,"given":"Talita D.","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziele Z.","family":"Manin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa D.","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7856-7512","authenticated-orcid":true,"given":"Dario S.","family":"Zamboni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2017,8,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1056\/NEJM197712012972201","article-title":"Legionnaires' disease: description of an epidemic of pneumonia","volume":"297","author":"DW Fraser","year":"1977","journal-title":"N Engl J Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.1084\/jem.158.6.2108","article-title":"The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes","volume":"158","author":"MA Horwitz","year":"1983","journal-title":"J Exp Med"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1056\/NEJM197712012972202","article-title":"Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease","volume":"297","author":"JE McDade","year":"1977","journal-title":"N Engl J Med"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1128\/CMR.00052-09","article-title":"Molecular pathogenesis of infections caused by Legionella pneumophila","volume":"23","author":"HJ Newton","year":"2010","journal-title":"Clin Microbiol Rev"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.meegid.2016.04.033","article-title":"Molecular epidemiology, phylogeny and evolution of Legionella","volume":"43","author":"A Khodr","year":"2016","journal-title":"Infect Genet Evol"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1365-2958.1993.tb01092.x","article-title":"Two distinct defects in intracellular growth complemented by a single genetic locus in Legionella pneumophila","volume":"7","author":"KH Berger","year":"1993","journal-title":"Mol Microbiol"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1111\/j.1365-2958.1994.tb01317.x","article-title":"Altered intracellular targeting properties associated with mutations in the Legionella pneumophila dotA gene","volume":"14","author":"KH Berger","year":"1994","journal-title":"Mol Microbiol"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1038\/nrmicro1967","article-title":"The Legionella pneumophila replication vacuole: making a cosy niche inside host cells","volume":"7","author":"RR Isberg","year":"2009","journal-title":"Nat Rev Microbiol"},{"key":"ref9","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1046\/j.1365-2958.1998.00841.x","article-title":"Legionella pneumophila DotA protein is required for early phagosome trafficking decisions that occur within minutes of bacterial uptake","volume":"28","author":"CR Roy","year":"1998","journal-title":"Mol Microbiol"},{"key":"ref10","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1146\/annurev.micro.54.1.567","article-title":"Legionella pneumophila pathogesesis: a fateful journey from amoebae to macrophages","volume":"54","author":"MS Swanson","year":"2000","journal-title":"Annu Rev Microbiol"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1128\/IAI.56.2.370-375.1988","article-title":"Growth of Legionella pneumophila in thioglycolate-elicited peritoneal macrophages from A\/J mice","volume":"56","author":"Y Yamamoto","year":"1988","journal-title":"Infect Immun"},{"key":"ref12","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1007\/BF00356173","article-title":"Natural resistance to infection with Legionella pneumophila: chromosomal localization of the Lgn1 susceptibility gene","volume":"6","author":"MC Beckers","year":"1995","journal-title":"Mamm Genome"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1016\/0888-7543(95)80161-E","article-title":"Lgn1, a gene that determines susceptibility to Legionella pneumophila, maps to mouse chromosome 13","volume":"26","author":"WF Dietrich","year":"1995","journal-title":"Genomics"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.4049\/jimmunol.164.3.1470","article-title":"The neuronal apoptosis inhibitory protein (Naip) is expressed in macrophages and is modulated after phagocytosis and during intracellular infection with Legionella pneumophila","volume":"164","author":"E Diez","year":"2000","journal-title":"J Immunol"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/ng1065","article-title":"Birc1e is the gene within the Lgn1 locus associated with resistance to Legionella pneumophila","volume":"33","author":"E Diez","year":"2003","journal-title":"Nat Genet"},{"key":"ref16","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/S0960-9822(02)01359-3","article-title":"Naip5 affects host susceptibility to the intracellular pathogen Legionella pneumophila","volume":"13","author":"EK Wright","year":"2003","journal-title":"Curr Biol"},{"key":"ref17","doi-asserted-by":"crossref","first-page":"35217","DOI":"10.1074\/jbc.M604933200","article-title":"Regulation of Legionella phagosome maturation and infection through flagellin and host Ipaf","volume":"281","author":"A Amer","year":"2006","journal-title":"J Biol Chem"},{"key":"ref18","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1084\/jem.20051659","article-title":"Cytosolic recognition of flagellin by mouse macrophages restricts Legionella pneumophila infection","volume":"203","author":"AB Molofsky","year":"2006","journal-title":"J Exp Med"},{"key":"ref19","doi-asserted-by":"crossref","first-page":"e18","DOI":"10.1371\/journal.ppat.0020018","article-title":"Flagellin-deficient Legionella mutants evade caspase-1- and Naip5-mediated macrophage immunity","volume":"2","author":"T Ren","year":"2006","journal-title":"PLoS Pathog"},{"key":"ref20","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/ni1305","article-title":"The Birc1e cytosolic pattern-recognition receptor contributes to the detection and control of Legionella pneumophila infection","volume":"7","author":"DS Zamboni","year":"2006","journal-title":"Nat Immunol"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"1981","DOI":"10.1128\/IAI.01382-08","article-title":"Asc and Ipaf Inflammasomes direct distinct pathways for caspase-1 activation in response to Legionella pneumophila","volume":"77","author":"CL Case","year":"2009","journal-title":"Infect Immun"},{"key":"ref22","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1038\/ni.1646","article-title":"Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin","volume":"9","author":"KL Lightfield","year":"2008","journal-title":"Nat Immunol"},{"key":"ref23","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1038\/ni.1960","article-title":"Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria","volume":"11","author":"EA Miao","year":"2010","journal-title":"Nat Immunol"},{"key":"ref24","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1128\/IAI.00905-09","article-title":"Pore formation triggered by Legionella spp. is an Nlrc4 inflammasome-dependent host cell response that precedes pyroptosis","volume":"78","author":"TN Silveira","year":"2010","journal-title":"Infect Immun"},{"key":"ref25","doi-asserted-by":"crossref","first-page":"e1003400","DOI":"10.1371\/journal.ppat.1003400","article-title":"Caspase-11 activation in response to bacterial secretion systems that access the host cytosol","volume":"9","author":"CN Casson","year":"2013","journal-title":"PLoS Pathog"},{"key":"ref26","article-title":"IL-1beta, IL-18, and eicosanoids promote neutrophil recruitment to pore-induced intracellular traps following pyroptosis","author":"I Jorgensen","year":"2016","journal-title":"Eur J Immunol"},{"key":"ref27","doi-asserted-by":"crossref","first-page":"e1003793","DOI":"10.1371\/journal.ppat.1003793","article-title":"Burning down the house: cellular actions during pyroptosis","volume":"9","author":"CN LaRock","year":"2013","journal-title":"PLoS Pathog"},{"key":"ref28","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1093\/infdis\/jiu430","article-title":"Interleukin 1 receptor-driven neutrophil recruitment accounts to MyD88-dependent pulmonary clearance of legionella pneumophila infection in vivo","volume":"211","author":"DP Mascarenhas","year":"2015","journal-title":"J Infect Dis"},{"key":"ref29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.3389\/fmicb.2011.00033","article-title":"The Nlrc4 Inflammasome Contributes to Restriction of Pulmonary Infection by Flagellated Legionella spp. that Trigger Pyroptosis","volume":"2","author":"MS Pereira","year":"2011","journal-title":"Front Microbiol"},{"key":"ref30","article-title":"Asc modulates the function of NLRC4 in response to infection of macrophages by Legionella pneumophila","volume":"2","author":"CL Case","year":"2011","journal-title":"MBio"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1038\/nature07725","article-title":"AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC","volume":"458","author":"V Hornung","year":"2009","journal-title":"Nature"},{"key":"ref32","doi-asserted-by":"crossref","first-page":"5239","DOI":"10.4049\/jimmunol.1301581","article-title":"Salmonella infection induces recruitment of Caspase-8 to the inflammasome to modulate IL-1beta production","volume":"191","author":"SM Man","year":"2013","journal-title":"J Immunol"},{"key":"ref33","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1038\/cdd.2012.51","article-title":"AIM2\/ASC triggers caspase-8-dependent apoptosis in Francisella-infected caspase-1-deficient macrophages","volume":"19","author":"R Pierini","year":"2012","journal-title":"Cell Death Differ"},{"key":"ref34","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1038\/cdd.2013.37","article-title":"AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASC","volume":"20","author":"V Sagulenko","year":"2013","journal-title":"Cell Death Differ"},{"key":"ref35","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.4049\/jimmunol.1501223","article-title":"Caspase-1 but Not Caspase-11 Is Required for NLRC4-Mediated Pyroptosis and Restriction of Infection by Flagellated Legionella Species in Mouse Macrophages and In Vivo","volume":"195","author":"DM Cerqueira","year":"2015","journal-title":"J Immunol"},{"key":"ref36","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1074\/jbc.M110.197681","article-title":"Apoptosis-associated speck-like protein (ASC) controls Legionella pneumophila infection in human monocytes","volume":"286","author":"DH Abdelaziz","year":"2011","journal-title":"J Biol Chem"},{"key":"ref37","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3389\/fmicb.2011.00018","article-title":"Asc-dependent and independent mechanisms contribute to restriction of legionella pneumophila infection in murine macrophages","volume":"2","author":"DH Abdelaziz","year":"2011","journal-title":"Front Microbiol"},{"key":"ref38","doi-asserted-by":"crossref","first-page":"6447","DOI":"10.4049\/jimmunol.1003784","article-title":"Activation of NLRC4 by flagellated bacteria triggers caspase-1-dependent and -independent responses to restrict Legionella pneumophila replication in macrophages and in vivo","volume":"187","author":"MS Pereira","year":"2011","journal-title":"J Immunol"},{"key":"ref39","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.1128\/IAI.65.6.2497-2501.1997","article-title":"Analysis of the Legionella pneumophila fliI gene: intracellular growth of a defined mutant defective for flagellum biosynthesis","volume":"65","author":"JJ Merriam","year":"1997","journal-title":"Infect Immun"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1038\/ni1346","article-title":"Cytosolic flagellin requires Ipaf for activation of caspase-1 and interleukin 1beta in salmonella-infected macrophages","volume":"7","author":"L Franchi","year":"2006","journal-title":"Nat Immunol"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-1-62703-523-1_8","article-title":"ASC speck formation as a readout for inflammasome activation","volume":"1040","author":"A Stutz","year":"2013","journal-title":"Methods Mol Biol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"33835","DOI":"10.1074\/jbc.274.48.33835","article-title":"ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells","volume":"274","author":"J Masumoto","year":"1999","journal-title":"J Biol Chem"},{"key":"ref43","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.1038\/sj.cdd.4402194","article-title":"The pyroptosome: a supramolecular assembly of ASC dimers mediating inflammatory cell death via caspase-1 activation","volume":"14","author":"T Fernandes-Alnemri","year":"2007","journal-title":"Cell Death Differ"},{"key":"ref44","doi-asserted-by":"crossref","first-page":"15122","DOI":"10.1074\/jbc.M412284200","article-title":"ASC-mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP","volume":"280","author":"M Hasegawa","year":"2005","journal-title":"J Biol Chem"},{"key":"ref45","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.chom.2010.11.007","article-title":"Differential requirement for Caspase-1 autoproteolysis in pathogen-induced cell death and cytokine processing","volume":"8","author":"P Broz","year":"2010","journal-title":"Cell Host Microbe"},{"key":"ref46","doi-asserted-by":"crossref","first-page":"29217","DOI":"10.1074\/jbc.M115.687731","article-title":"The Inflammasome Adaptor ASC Induces Procaspase-8 Death Effector Domain Filaments","volume":"290","author":"PR Vajjhala","year":"2015","journal-title":"J Biol Chem"},{"key":"ref47","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0006-291X(03)00309-7","article-title":"ASC is an activating adaptor for NF-kappa B and caspase-8-dependent apoptosis","volume":"303","author":"J Masumoto","year":"2003","journal-title":"Biochem Biophys Res Commun"},{"key":"ref48","doi-asserted-by":"crossref","first-page":"e1000361","DOI":"10.1371\/journal.ppat.1000361","article-title":"Caspase-7 activation by the Nlrc4\/Ipaf inflammasome restricts Legionella pneumophila infection","volume":"5","author":"A Akhter","year":"2009","journal-title":"PLoS Pathog"},{"key":"ref49","doi-asserted-by":"crossref","first-page":"7403","DOI":"10.1073\/pnas.1402911111","article-title":"Inflammasome activation causes dual recruitment of NLRC4 and NLRP3 to the same macromolecular complex","volume":"111","author":"SM Man","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref50","doi-asserted-by":"crossref","first-page":"33963","DOI":"10.1074\/jbc.M111.286823","article-title":"Caspase-1 protein induces apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC)-mediated necrosis independently of its catalytic activity","volume":"286","author":"K Motani","year":"2011","journal-title":"J Biol Chem"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1042\/BJ20091349","article-title":"Interaction with Sug1 enables Ipaf ubiquitination leading to caspase 8 activation and cell death","volume":"427","author":"Y Kumar","year":"2010","journal-title":"Biochem J"},{"key":"ref52","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1038\/nature06664","article-title":"The inflammasome recognizes cytosolic microbial and host DNA and triggers an innate immune response","volume":"452","author":"DA Muruve","year":"2008","journal-title":"Nature"},{"key":"ref53","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1126\/science.1169841","article-title":"HIN-200 proteins regulate caspase activation in response to foreign cytoplasmic DNA","volume":"323","author":"TL Roberts","year":"2009","journal-title":"Science"},{"key":"ref54","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/ni.1702","article-title":"An orthogonal proteomic-genomic screen identifies AIM2 as a cytoplasmic DNA sensor for the inflammasome","volume":"10","author":"T Burckstummer","year":"2009","journal-title":"Nat Immunol"},{"key":"ref55","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/nature07710","article-title":"AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA","volume":"458","author":"T Fernandes-Alnemri","year":"2009","journal-title":"Nature"},{"key":"ref56","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1126\/science.1230751","article-title":"Caspase-11 protects against bacteria that escape the vacuole","volume":"339","author":"Y Aachoui","year":"2013","journal-title":"Science"},{"key":"ref57","doi-asserted-by":"crossref","first-page":"6193","DOI":"10.1073\/pnas.1117490109","article-title":"Preventing bacterial DNA release and absent in melanoma 2 inflammasome activation by a Legionella effector functioning in membrane trafficking","volume":"109","author":"J Ge","year":"2012","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref58","doi-asserted-by":"crossref","first-page":"3847","DOI":"10.4049\/jimmunol.1203326","article-title":"ASC controls IFN-gamma levels in an IL-18-dependent manner in caspase-1-deficient mice infected with Francisella novicida","volume":"191","author":"R Pierini","year":"2013","journal-title":"J Immunol"},{"key":"ref59","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/nature09857","article-title":"RIP3 mediates the embryonic lethality of caspase-8-deficient mice","volume":"471","author":"WJ Kaiser","year":"2011","journal-title":"Nature"},{"key":"ref60","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S1074-7613(00)80609-3","article-title":"Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas\/Apo1, and DR3 and is lethal prenatally","volume":"9","author":"EE Varfolomeev","year":"1998","journal-title":"Immunity"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1073\/pnas.1211521110","article-title":"Caspase-11 stimulates rapid flagellin-independent pyroptosis in response to Legionella pneumophila","volume":"110","author":"CL Case","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"ref62","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1111\/j.1462-5822.2006.00751.x","article-title":"Caspase-1-dependent pore formation during pyroptosis leads to osmotic lysis of infected host macrophages","volume":"8","author":"SL Fink","year":"2006","journal-title":"Cell Microbiol"},{"key":"ref63","doi-asserted-by":"crossref","first-page":"9677","DOI":"10.1074\/jbc.272.15.9677","article-title":"Substrate specificities of caspase family proteases","volume":"272","author":"RV Talanian","year":"1997","journal-title":"J Biol Chem"},{"key":"ref64","doi-asserted-by":"crossref","first-page":"17907","DOI":"10.1074\/jbc.272.29.17907","article-title":"A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis","volume":"272","author":"NA Thornberry","year":"1997","journal-title":"J Biol Chem"},{"key":"ref65","doi-asserted-by":"crossref","first-page":"32608","DOI":"10.1074\/jbc.273.49.32608","article-title":"Inhibition of human caspases by peptide-based and macromolecular inhibitors","volume":"273","author":"M Garcia-Calvo","year":"1998","journal-title":"J Biol Chem"},{"key":"ref66","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1038\/nature10558","article-title":"Non-canonical inflammasome activation targets caspase-11","volume":"479","author":"N Kayagaki","year":"2011","journal-title":"Nature"},{"key":"ref67","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/nature15541","article-title":"Caspase-11 cleaves gasdermin D for non-canonical inflammasome signalling","volume":"526","author":"N Kayagaki","year":"2015","journal-title":"Nature"},{"key":"ref68","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1038\/nature15514","article-title":"Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death","volume":"526","author":"J Shi","year":"2015","journal-title":"Nature"},{"key":"ref69","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1038\/nature10394","article-title":"Innate immune recognition of bacterial ligands by NAIPs determines inflammasome specificity","volume":"477","author":"EM Kofoed","year":"2011","journal-title":"Nature"},{"key":"ref70","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1038\/nature10510","article-title":"The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus","volume":"477","author":"Y Zhao","year":"2011","journal-title":"Nature"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1111\/j.1462-5822.2007.00963.x","article-title":"Restriction of Legionella pneumophila growth in macrophages requires the concerted action of cytokine and Naip5\/Ipaf signalling pathways","volume":"9","author":"J Coers","year":"2007","journal-title":"Cell Microbiol"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"4794","DOI":"10.1128\/IAI.01546-08","article-title":"Restriction of Legionella pneumophila replication in macrophages requires concerted action of the transcriptional regulators Irf1 and Irf8 and nod-like receptors Naip5 and Nlrc4","volume":"77","author":"A Fortier","year":"2009","journal-title":"Infect Immun"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/j.immuni.2017.03.016","article-title":"NAIP-NLRC4 Inflammasomes Coordinate Intestinal Epithelial Cell Expulsion with Eicosanoid and IL-18 Release via Activation of Caspase-1 and -8","volume":"46","author":"I Rauch","year":"2017","journal-title":"Immunity"},{"key":"ref74","doi-asserted-by":"crossref","first-page":"14128","DOI":"10.1038\/ncomms14128","article-title":"Cleavage of DFNA5 by caspase-3 during apoptosis mediates progression to secondary necrotic\/pyroptotic cell death","volume":"8","author":"C Rogers","year":"2017","journal-title":"Nat Commun"},{"key":"ref75","article-title":"Chemotherapy drugs induce pyroptosis through caspase-3 cleavage of a Gasdermin","author":"Y Wang","year":"2017","journal-title":"Nature"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1128\/JCM.10.4.437-441.1979","article-title":"Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila","volume":"10","author":"JC Feeley","year":"1979","journal-title":"J Clin Microbiol"},{"key":"ref77","doi-asserted-by":"crossref","first-page":"e15263","DOI":"10.1371\/journal.pone.0015263","article-title":"A method for generation of bone marrow-derived macrophages from cryopreserved mouse bone marrow cells","volume":"5","author":"FM Marim","year":"2010","journal-title":"PLoS One"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/B978-0-12-420067-8.00006-4","article-title":"Measuring RNAi knockdown using qPCR","volume":"533","author":"S Milstein","year":"2013","journal-title":"Methods Enzymol"},{"key":"ref79","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1084\/jem.20060206","article-title":"Role of the caspase-1 inflammasome in Salmonella typhimurium pathogenesis","volume":"203","author":"M Lara-Tejero","year":"2006","journal-title":"J Exp Med"},{"key":"ref80","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1126\/science.7535475","article-title":"Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme","volume":"267","author":"K Kuida","year":"1995","journal-title":"Science"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.immuni.2006.02.004","article-title":"Critical role for NALP3\/CIAS1\/Cryopyrin in innate and adaptive immunity through its regulation of caspase-1","volume":"24","author":"FS Sutterwala","year":"2006","journal-title":"Immunity"}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1006502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,14]],"date-time":"2020-10-14T00:47:48Z","timestamp":1602636468000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1006502"}},"subtitle":[],"editor":[{"given":"H. Steven","family":"Seifert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,8,3]]},"references-count":81,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2017,8,3]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.ppat.1006502","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.727873808.793536189","asserted-by":"object"}]},"ISSN":["1553-7374"],"issn-type":[{"value":"1553-7374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,3]]}}}