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Zhou","year":"2010","journal-title":"PLoS One"},{"key":"ref40","doi-asserted-by":"crossref","first-page":"4846","DOI":"10.1242\/jcs.148015","article-title":"The bi-lobe-associated LRRP1 regulates Ran activity in Trypanosoma brucei","volume":"127","author":"A Brasseur","year":"2014","journal-title":"J Cell Sci"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.molbiopara.2009.05.001","article-title":"The bilobe structure of Trypanosoma brucei contains a MORN-repeat protein","volume":"167","author":"B Morriswood","year":"2009","journal-title":"Mol Biochem Parasitol"},{"key":"ref42","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1128\/EC.00094-15","article-title":"A MORN Repeat Protein Facilitates Protein Entry into the Flagellar Pocket of Trypanosoma brucei","volume":"14","author":"B Morriswood","year":"2015","journal-title":"Eukaryot 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Parasitol"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"140197","DOI":"10.1098\/rsob.140197","article-title":"A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids","volume":"5","author":"S Dean","year":"2015","journal-title":"Open Biol"},{"key":"ref72","doi-asserted-by":"crossref","first-page":"985","DOI":"10.2307\/3282883","article-title":"Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers","volume":"75","author":"H Hirumi","year":"1989","journal-title":"J Parasitol"},{"key":"ref73","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/319511a0","article-title":"A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains","volume":"319","author":"CH Heldin","year":"1986","journal-title":"Nature"},{"key":"ref74","first-page":"39","article-title":"Down-regulating gene expression by RNA interference in Trypanosoma brucei","volume":"309","author":"CE Clayton","year":"2005","journal-title":"Methods Mol Biol"},{"key":"ref75","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.molbiopara.2005.09.002","article-title":"A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genes","volume":"145","author":"M Oberholzer","year":"2006","journal-title":"Mol Biochem Parasitol"},{"key":"ref76","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.molbiopara.2010.03.007","article-title":"Selection of reference genes for mRNA quantification in Trypanosoma brucei","volume":"172","author":"M Brennd\u00f6rfer","year":"2010","journal-title":"Mol Biochem Parasitol"},{"key":"ref77","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1550-7408.1999.tb04592.x","article-title":"Assembly of the paraflagellar rod and the flagellum attachment zone complex during the Trypanosoma brucei cell cycle","volume":"46","author":"L Kohl","year":"1999","journal-title":"J Eukaryot Microbiol"},{"key":"ref78","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0166-6851(96)02598-4","article-title":"A novel epitope tag system to study protein targeting and organelle biogenesis in Trypanosoma brucei","volume":"77","author":"P Bastin","year":"1996","journal-title":"Mol Biochem Parasitol"},{"key":"ref79","article-title":"Human SAXO1 (FAM154A) is a microtubule-stabilizing protein specific to cilia and related structures","author":"D Dacheux","year":"2015","journal-title":"J Cell Sci"},{"key":"ref80","doi-asserted-by":"crossref","first-page":"e31344","DOI":"10.1371\/journal.pone.0031344","article-title":"A MAP6-Related Protein Is Present in Protozoa and Is Involved in Flagellum Motility","volume":"7","author":"D Dacheux","year":"2012","journal-title":"PLoS One"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"F Sievers","year":"2011","journal-title":"Mol Syst Biol"}],"updated-by":[{"DOI":"10.1371\/journal.ppat.1006710","type":"new_version","label":"New version","source":"publisher","updated":{"date-parts":[[2017,11,13]],"date-time":"2017-11-13T00:00:00Z","timestamp":1510531200000}}],"container-title":["PLOS Pathogens"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/dx.plos.org\/10.1371\/journal.ppat.1006710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T23:14:20Z","timestamp":1659741260000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.ppat.1006710"}},"subtitle":[],"editor":[{"given":"Keith 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